LOCUS       NM_001398964           14381 bp    mRNA    linear   ROD 09-APR-2024
DEFINITION  Rattus norvegicus epiplakin 1 (Eppk1), mRNA.
ACCESSION   NM_001398964 XM_017595389
VERSION     NM_001398964.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 14381)
  AUTHORS   Kokado,M., Okada,Y., Miyamoto,T., Yamanaka,O. and Saika,S.
  TITLE     Effects of epiplakin-knockdown in cultured corneal epithelial cells
  JOURNAL   BMC Res Notes 9, 278 (2016)
   PUBMED   27206504
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14381)
  AUTHORS   Szabo,S., Wogenstein,K.L., Osterreicher,C.H., Guldiken,N., Chen,Y.,
            Doler,C., Wiche,G., Boor,P., Haybaeck,J., Strnad,P. and Fuchs,P.
  TITLE     Epiplakin attenuates experimental mouse liver injury by chaperoning
            keratin reorganization
  JOURNAL   J Hepatol 62 (6), 1357-1366 (2015)
   PUBMED   25617501
REFERENCE   3  (bases 1 to 14381)
  AUTHORS   Wogenstein,K.L., Szabo,S., Lunova,M., Wiche,G., Haybaeck,J.,
            Strnad,P., Boor,P., Wagner,M. and Fuchs,P.
  TITLE     Epiplakin deficiency aggravates murine caerulein-induced acute
            pancreatitis and favors the formation of acinar keratin granules
  JOURNAL   PLoS One 9 (9), e108323 (2014)
   PUBMED   25232867
  REMARK    Publication Status: Online-Only
REFERENCE   4  (bases 1 to 14381)
  AUTHORS   Kokado,M., Okada,Y., Goto,M., Ishikawa,K., Miyamoto,T.,
            Yamanaka,O., Fujiwara,S. and Saika,S.
  TITLE     Increased fragility, impaired differentiation, and acceleration of
            migration of corneal epithelium of epiplakin-null mice
  JOURNAL   Invest Ophthalmol Vis Sci 54 (5), 3780-3789 (2013)
   PUBMED   23599337
  REMARK    Publication Status: Online-Only
REFERENCE   5  (bases 1 to 14381)
  AUTHORS   Shimada,H., Nambu-Niibori,A., Wilson-Morifuji,M., Mizuguchi,S.,
            Araki,N., Sumiyoshi,H., Sato,M., Mezaki,Y., Senoo,H., Ishikawa,K.,
            Hatano,Y., Okamoto,O. and Fujiwara,S.
  TITLE     Epiplakin modifies the motility of the HeLa cells and accumulates
            at the outer surfaces of 3-D cell clusters
  JOURNAL   J Dermatol 40 (4), 249-258 (2013)
   PUBMED   23398049
REFERENCE   6  (bases 1 to 14381)
  AUTHORS   Spazierer,D., Raberger,J., Gross,K., Fuchs,P. and Wiche,G.
  TITLE     Stress-induced recruitment of epiplakin to keratin networks
            increases their resistance to hyperphosphorylation-induced
            disruption
  JOURNAL   J Cell Sci 121 (Pt 6), 825-833 (2008)
   PUBMED   18285451
REFERENCE   7  (bases 1 to 14381)
  AUTHORS   Wang,W., Sumiyoshi,H., Yoshioka,H. and Fujiwara,S.
  TITLE     Interactions between epiplakin and intermediate filaments
  JOURNAL   J Dermatol 33 (8), 518-527 (2006)
   PUBMED   16923132
  REMARK    Erratum:[J Dermatol. 2006 Sep;33(9):654]
REFERENCE   8  (bases 1 to 14381)
  AUTHORS   Goto,M., Sumiyoshi,H., Sakai,T., Fassler,R., Ohashi,S., Adachi,E.,
            Yoshioka,H. and Fujiwara,S.
  TITLE     Elimination of epiplakin by gene targeting results in acceleration
            of keratinocyte migration in mice
  JOURNAL   Mol Cell Biol 26 (2), 548-558 (2006)
   PUBMED   16382146
REFERENCE   9  (bases 1 to 14381)
  AUTHORS   Jang,S.I., Kalinin,A., Takahashi,K., Marekov,L.N. and Steinert,P.M.
  TITLE     Characterization of human epiplakin: RNAi-mediated epiplakin
            depletion leads to the disruption of keratin and vimentin IF
            networks
  JOURNAL   J Cell Sci 118 (Pt 4), 781-793 (2005)
   PUBMED   15671067
REFERENCE   10 (bases 1 to 14381)
  AUTHORS   Spazierer,D., Fuchs,P., Proll,V., Janda,L., Oehler,S., Fischer,I.,
            Hauptmann,R. and Wiche,G.
  TITLE     Epiplakin gene analysis in mouse reveals a single exon encoding a
            725-kDa protein with expression restricted to epithelial tissues
  JOURNAL   J Biol Chem 278 (34), 31657-31666 (2003)
   PUBMED   12791695
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            JAXUCZ010000007.1.
            
            On Dec 30, 2021 this sequence version replaced XM_017595389.2.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR26360190.2189011.1,
                                           SRR26360172.2963346.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA5760400, SAMEA5760476
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-94                JAXUCZ010000007.1  109718375-109718468 c
            95-14381            JAXUCZ010000007.1  109697607-109711893 c
FEATURES             Location/Qualifiers
     source          1..14381
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="BN"
                     /db_xref="taxon:10116"
                     /chromosome="7"
                     /map="7q34"
     gene            1..14381
                     /gene="Eppk1"
                     /gene_synonym="Eppk1-ps1"
                     /note="epiplakin 1"
                     /db_xref="GeneID:680860"
                     /db_xref="RGD:1587023"
     exon            1..94
                     /gene="Eppk1"
                     /gene_synonym="Eppk1-ps1"
                     /inference="alignment:Splign:2.1.0"
     exon            95..14381
                     /gene="Eppk1"
                     /gene_synonym="Eppk1-ps1"
                     /inference="alignment:Splign:2.1.0"
     CDS             141..13607
                     /gene="Eppk1"
                     /gene_synonym="Eppk1-ps1"
                     /note="epiplakin 1, pseudogene 1"
                     /codon_start=1
                     /product="epiplakin"
                     /protein_id="NP_001385893.1"
                     /db_xref="GeneID:680860"
                     /db_xref="RGD:1587023"
                     /translation="MNGQAPPHDVVVANGTEKLIVPKIMKNQSEASTPSRPQAKAALP
                     TTARSIAGVYVEASGQTLSIYDAMKQGLLPTGLGVTLLEAQAATGGLMDLAQGQLLPV
                     SEALRRGLVGLELKEKLLAAERAVTGYPDPYGGEKLSLFQAIRKEVVDKTLGWRLLEA
                     QLATGGLVDPTQGVQVAPELACQQGLLDKETWLSLMESEPSMGTLGFSDPNTLEQLPY
                     SVLLGRCVLDPSSGLPLLPLKTTFHTLAGAASAAMLLEAGVLNEETVRGLQEGTLVVS
                     DVGTRPEVQRYLEGTGGLAGVVLLPGGHKKSFFQATVEHSLSKGIALQLLEAQAATRT
                     LVHPSTGQRLWVEEAVKTGLVGPDLHEQLLVAEQAVTGYYDPFSSSRIPLFQAMKKGL
                     VDQPLALRLLDAQVATGGLICPARRFRLPLEAALRFGCLDEETRQRLSQTMGFSDPTT
                     HDRLHYEQLLALSVTDPETGLAFLPLLGMSHANEPQGLPFIDHCTRHVLSKATTSVSV
                     GRYQGRPVSLWELLFSEAVPVKQRAMLAQKHQNGALSVEALAAELKSIVQQAAATAKV
                     TFAGLRDTVTPGELLKAEIINQDLFEQLERGQTSARDVGSLDSVQRYLQGTGCIAGLL
                     LPDSQERLRIYEARSKGLLRPGTALILLEAQAATGFIIDPKENKRYSVEEALRAGVIG
                     LDVYAKLLSAERAVTGYTDPYSGEQISLFQAMQRELIVRDHGIRLLEAQIATGGVIDP
                     VHSHRVPVDVAYQRGYFDQMLNSILLDPSDDTKGFFDPNTHENLTYLQLLERCIHDSE
                     TGLHLLPLSGTRPQLVDSSTRQAFQNLLISVKYGRFRGQRVSAWELINSEYFKEDWRR
                     QLLRRCQQRKITLEQVTQLLEKEMRRWADVTLPALQGQVTAYQLLEAHIINQELLDQV
                     LTGTVSPDALLHMSDVRRYLQGSGTVGGVLLQPSNQRISLYQAMKQKLLAPSVALALL
                     EAQAATGAITDPCSMQTLSVDEAVCRGVVGAEVYHKLKQAEHSITGYRDPFSGKTVSL
                     FGAMKKGLVPVEQATRLLEAQVSTGGVVDPTTHLHLPMSVAVQRGCIDQEMEVALSRS
                     PETFPTPDGRGHASYAQLLEQCLQDEPSGLHLLPLTEDAPSVPTDTQIQESLQALAGT
                     EDGLSLWDSLTSCHFTEEQRNGFLEDVRVGKTSVSQLQDIVRSWVQTAKLLARARITV
                     PGPQGEVPAVWLRDAGIITQETLEALAQGVQSPDEVAEQPAVKACLWGTGCVAGVLLQ
                     PSGTKLSIAQAVRDGLLPAGLGQQLLEAQVASGFLVNPLTNQRLSVHDAVKAGLVGVE
                     LSEQLRQVERAVTGYSDPFSGGLLSLWQAKEKGLVSQSEAFPLLQVQLATGGVVDPVH
                     RVHLPQAVAYKLGLLDEQTSQALTATGEENKLFFDPNSREKVTYQQLKELCVLDADTG
                     LWLLPLPQDTVLEVDDHTAVALRAMKVPINMGRFQGHSVSLWDLLHSEYVGAEKRREL
                     VALCCSGRAAALRQVISMVTTLVEAAEKQPPQATFKGLRKQVSAGDLFRSQLITKQTL
                     DELNQGKRTVEEVTEMDSVRRSLEGGNFIAGVLIRDTKEKMSIPEALRRHILRPGTAL
                     VLLEAQAATGFIIDPVENRKLTVEQAFQAGMFGKETYTKLLSAERAVTGYTDPYTGEQ
                     ISLFQAMQRDLIVRDHGIRLLEAQIATGGIIDPVHSHRVPVDVAYQRGYFNEEMNRVL
                     ADPSDDTKGFFDPNTHENLTYLQLLERCVEDPETGLYMLEIVKKGEIYVYIDEATRQV
                     LRSRTMKMHIGKFAGQTVSVWDLLSSQYFTEGRKRKLLREYRAQNIGLEKLLEVITST
                     VEDTEKQSQIFKVPGIHGDVTAVELFNSGILDKRTLDALHSGERGRQDLCQLEHIKIY
                     LEGTNCIAGVTAPLTQKVMSFYKASKEELIPAGFAAQLLEAQAATGYLMDPCSRRRLC
                     VDEAIAAGLVGEDLRERLVSAEKAAKGYEDPATGETLPLFQAMEKKLVGREEALRLLE
                     VQVATGGVIDPLHRHRIPLDVACQRGCLCEDIIVLIADQKHMRRRFVDPNTQEKVTYQ
                     ELQDRCRKEEKSGWALFPVVKDKKDMDYVDEATKRALEAEQVEVTVGRYRGQKPSVWE
                     LLNSEYFSEEKKIELVRIYKEDTARALQKVVKLILKIIADKEGRSRQLWFRGLRTQVT
                     AEELLRAAVITRKTLEDLEEGRATVDQIEETEDVKRYLKGTSCIAGILVPVQGEPGRQ
                     EKMSIYQAMWKGLLRPGTALVLLEAQAATGFIIDPVHNRRLSVEEAVAAGVVGGEIQE
                     KLLAAERAVTGYTDPYTGEQISLFQAMQRDLIVKDHGIRLLEAQIATGGVIDPVHSHR
                     VPVDVAYKRGYFDEEMNSILADPGDDTKGFFDPNTHENLTYLQLLRRCVRDPETGFYM
                     LQLAGKGSSVYHVSEDLRRALREARVSPGTVDFQGQSISVWELLFYREVPESLRQDLL
                     RRYQAGGLSVQDVSTTLTSLLARTKEGSPRADPQGTLGKATMEVKRGHLRGHEVPVWD
                     VLTSNYVSRDTRKELLAQFSSGALTLPMLKRRLTTIIEETEETQESQAGHSVTSLKQQ
                     ESAAGSSGAYADKGEAGDSTDSARQQQEQTLRAATMQVRRGQFRDQPVSVWKVLFSSY
                     LSETRREELLSQHLAGRLGVNELISILVQVIEETEERLSRVSLPGLRRQVTVSELGNT
                     GILDPDTMRELAQGTRTIDEVTEMDSVKRYLKGTSCIAGILVPVQGEPGRQEKMSIYQ
                     AMWKGLLRPGTALVLLEAQAATGFIIDPVHNRRLSVEEAVAAGVVGGEIQEKLLAAER
                     AVTGYTDPYTGEQISLFQAMQRDLIVKDHGIRLLEAQIATGGVIDPVHSHRVPVDVAY
                     KRGYFDEEMNSILADPGDDTKGFFDPNTHENLTYLQLLRRCVRDPETGFYMLQLAGKG
                     SSVYHVSEDLRRALREARVSPGTVDFQGQSISVWELLFYREVPESLRQDLLRRYQAGG
                     LSVQDVSTTLTSLLARTKEGSPRADPQGTLGKATMEVKRGHLRGHEVPVWDVLTSNYV
                     SRDTRKELLAQFSSGALTLPMLKRRLTTIIEETEETQESQAGHSVTSLKQQESAAGSS
                     GAYADKGEAGDSTDSARQQQEQTLRAATMQVRRGQFRDQPVSVWKVLFSSYLSETRRE
                     ELLSQHLAGRLGVNELISILVQVIEETEERLSRVSLPGLRRQVTVSELGNTGILDPDT
                     MRELAQGTRTIDEVTEMDSVKRYLKGTSCIAGILVPVQGEPGRQEKMSIYQAMWKGLL
                     RPGTALVLLEAQAATGFIIDPVHNRRLSVEEAVAAGVVGGEIQEKLLAAERAVTGYTD
                     PYTGEQISLFQAMQRDLIVKDHGIRLLEAQIATGGVIDPVHSHRVPVDVAYKRGYFDE
                     EMNSILADPGDDTKGFFDPNTHENLTYLQLLRRCVRDPETGFYMLQLAGKGSSVYHVS
                     EDLRRALREARVSPGTVDFQGQSISVWELLFYREVPESLRQDLLRRYQAGGLSVQDVS
                     TTLTSLLARTKEGSPRADPQGTLGKATMEVKRGHLRGHEVPVWDVLTSNYVSRDTRKE
                     LLAQFSSGALTLPMLKRRLTTIIEETEETQESQAGHSVTSLKQQESAAGSSGAYADKG
                     EAGDSTDSARQQQEQTLRAATMQVRRGQFRDQPVSVWKVLFSSYLSETRREELLSQHL
                     AGRLGVNELISILVQVIEETEERLSRVSLPGLRRQVTVSELGNTGILDPDTMRELAQG
                     TRTIDEVTEMDSVKRYLKGTSCIAGILVPVQGEPGRQEKMSIYQAMWKGLLRPGTALV
                     LLEAQAATGFIIDPVHNRRLSVEEAVAAGVVGGEIQEKLLAAERAVTGYTDPYTGEQI
                     SLFQAMQRDLIVKDHGIRLLEAQIATGGVIDPVHSHRVPVDVAYKRGYFDEEMNSILA
                     DPGDDTKGFFDPNTHENLTYLQLLRRCVRDPETGFYMLQLAGKGSSVYHVSEDLRRAL
                     REARVSPGTVDFQGQSISVWELLFYREVPESLRQDLLRRYQAGGLSVQDVSTTLTSLL
                     ARTKEGSPRADPQGTLGKATMEVKRGHLRGHEVPVWDVLTSNYVSRDTRKELLAQFSS
                     GALTLPMLKRRLTTIIEETEETQESQAGHSVTSLKQQESAAGSSGAYADKGEAGDSTD
                     SARQQQEQTLRAATMQVRRGQFRDQPVSVWKVLFSSYLSETRREELLSQHLAGRLGVN
                     ELISILVQVIEETEERLSRVSFPGLRRQVTASELSITGILDPDTMRELAQGTRTIDEV
                     TEMDSVKRYLKGTSCIAGILVPVQGEPGRQEKMSIYQAMWKGLLRPGTALVLLEAQAA
                     TGFIIDPVHNRRLSVEEAVAEGVVGGEIREKLLSAERAVTGYTDPYTGEQISLFQAMQ
                     RDLIVKNHGIRLLEAQIATGGVIDPVHSHRVPVDVAYKRGYFDEEMNSILADPGDDTK
                     GFFDPNTHENLTYLQLLQRATVEPETGLLFLSLLKG"
ORIGIN      
        1 ggggccaatg cagatttaaa tgcctgtggc gcggggctgc cggtgtggag ggagcagaga
       61 gactgaggac actgcgccag ccttgttcca acaggacaca agtggactgt aggcaagttc
      121 tgcgctacta gttgtccacg atgaatggcc aggcccctcc tcatgatgtc gtagttgcca
      181 atgggactga gaaattaatt gtccctaaaa tcatgaagaa ccaatcggaa gccagcactc
      241 cttcgaggcc ccaagcaaag gctgccctgc ctactacagc cagaagtatt gctggggtat
      301 atgtggaggc ctcagggcag accctgagca tctatgatgc aatgaagcag ggcctcctgc
      361 ccactgggct tggggtgact ttgcttgagg cccaggcagc cactggaggc ctcatggacc
      421 ttgcccaggg ccagcttctt cctgtgtctg aggctctaag gcggggtctg gtgggactgg
      481 aactaaagga gaaattgttg gctgctgaac gtgcggttac tggctatccc gacccctatg
      541 ggggtgaaaa gctgtccctc ttccaggcca tcaggaaaga agttgtagac aagaccctgg
      601 ggtggagatt gctagaggcc cagctagcta ctgggggcct ggtggaccca acccagggtg
      661 ttcaagtagc cccagagcta gcctgtcagc agggcctttt ggataaggag acatggctca
      721 gcctgatgga gtcagagccc agtatgggta ccctgggctt ttctgacccc aacacactag
      781 agcagctgcc atactctgtg ctgttgggta ggtgtgtgct agaccccagc tcagggctgc
      841 ccctattgcc cttaaagacc accttccaca ccctggctgg agcagccagt gcggcaatgc
      901 tgctggaggc aggggtacta aatgaggaga cagtccgagg cctgcaggag ggcacactgg
      961 tggtgtcaga tgtgggcaca cgtcccgagg tgcaacgcta tctggaaggc actggagggt
     1021 tagcgggggt tgtcctgttg cctggaggtc acaagaaaag ctttttccag gctacggttg
     1081 aacactcgct ctcaaagggc attgctctac aactcttaga ggctcaggct gccacccgca
     1141 ccctcgtgca cccaagcaca ggccagcgtc tttgggtgga agaggcagtc aagacaggcc
     1201 tggttggccc agaccttcat gagcagctcc tggttgcaga acaggcagtg actggctatt
     1261 acgacccctt cagcagctcc cgcatccccc ttttccaggc tatgaagaag gggcttgtgg
     1321 accagccact ggctctgcgc ctcctggatg cccaggtagc cacaggtggg cttatatgcc
     1381 cagcccgcag attccggctg cccctggagg ctgccctgcg ctttggctgt ctagatgaag
     1441 agactcggca acgtctctct cagacaatgg gcttttcaga tcctaccaca catgaccgtc
     1501 ttcactatga acagctgttg gccctctctg tcactgaccc agagacagga cttgctttcc
     1561 tgcctctcct tggaatgtcc catgcaaatg agcctcaggg actcccattt attgatcact
     1621 gtacccgaca tgtcttgagc aaggccacga cctccgtctc tgtagggagg taccagggcc
     1681 ggcctgtctc cctctgggaa ctgctctttt ctgaggcggt gcccgttaag cagagagcaa
     1741 tgcttgccca gaagcaccag aacggggctc tgtcagtgga ggcgctggca gctgagctga
     1801 agagcattgt tcagcaggct gcagcaactg ccaaagtcac ctttgctggg ttgagggaca
     1861 ctgtgacacc gggcgagctg ttgaaggctg agataatcaa ccaggacctg tttgagcagc
     1921 tggagcgtgg acagacctca gcccgagatg tgggcagcct ggactcagtg cagaggtacc
     1981 tgcagggcac aggttgcatt gcaggccttc tgcttcctga ctcccaggaa cgtctcagga
     2041 tctatgaggc tcgtagcaag gggctcctca ggcctggcac tgccctgatc ttgcttgagg
     2101 cccaggctgc cacaggcttt atcattgacc caaaagagaa caagagatac tctgtagagg
     2161 aggcgctgag ggctggtgtc attgggcttg atgtctatgc aaagctccta tctgcagaac
     2221 gtgccgtgac tggctacacg gacccctact ctggggagca gatctccctc ttccaggcca
     2281 tgcagagaga acttattgtc agagaccacg gcatccgcct gctggaggcc cagatcgcca
     2341 ccggtggtgt cattgaccct gtgcacagtc accgtgtgcc cgtggacgtg gcctaccaac
     2401 gtggctactt tgatcagatg ctgaactcta ttctgctgga cccgtctgat gacaccaagg
     2461 gcttctttga ccccaacacg catgaaaatc tcacctacct gcagcttctg gagcgctgta
     2521 tacatgactc tgagacgggc ctccacctcc tgccccttag tggtacacga ccccagctgg
     2581 tagatagctc cactcggcag gccttccaga atctactgat ctcagtgaaa tacgggcggt
     2641 tccgggggca gagggtttct gcgtgggaac tgataaactc tgaatatttc aaagaggact
     2701 ggaggagaca gctactgcgg cgctgtcagc agcgcaagat cacattggag caggtcactc
     2761 agctgctgga gaaagagatg aggaggtggg cagatgtcac actgcctgcc ctacagggcc
     2821 aggtcactgc ctaccagctc ctagaggccc acatcatcaa ccaggagctc ctggaccagg
     2881 tactgacagg gacggtcagc ccagatgccc tccttcacat gagcgatgtt cgcaggtacc
     2941 ttcagggctc aggcaccgtg ggtggtgtgc tattacaacc ctccaaccag cgaatcagtc
     3001 tctaccaggc catgaagcag aagctgctgg cgcctagtgt agctttggcc ttgctggagg
     3061 cccaggcagc cactggggcc atcaccgacc cctgcagtat gcagaccctg tcagtagatg
     3121 aagctgtgtg ccggggagtg gtgggagcag aagtgtacca caagctgaag caagcagaac
     3181 attctatcac tggttacaga gatccctttt ctgggaaaac ggtatccctg ttcggggcca
     3241 tgaagaaggg ccttgttcct gtggagcagg caacccgcct actagaggcc caggtgtcca
     3301 caggaggggt cgttgacccc acaacccacc tccacctccc catgtctgtg gccgttcagc
     3361 gcggttgcat cgatcaagag atggaggtgg ccttgtccag atctcctgag acctttccca
     3421 cacccgatgg ccgggggcat gccagctatg cccagcttct ggaacagtgt ctccaggatg
     3481 aaccctctgg gcttcacctc ctgcccctga cagaagatgc ccccagcgtc cccactgaca
     3541 cacagatcca ggagagcctg caagcattgg ctggcactga agatggtttg tccctctggg
     3601 actcgcttac ctcttgtcac tttaccgagg agcagcgcaa tggcttcctt gaagatgtga
     3661 gggttgggaa gacatctgtg tcacagctac aggacattgt acgcagctgg gtgcaaacag
     3721 caaagctcct ggcccgggcc cgcatcaccg taccgggccc acagggtgag gtccctgccg
     3781 tttggctacg agatgctggt attatcaccc aagagacact ggaagctctg gcacagggcg
     3841 ttcagtcacc ggatgaggta gctgaacagc ctgctgtgaa ggcctgcctc tggggtacag
     3901 gctgtgtggc cggtgtgctg ctccagccct cagggaccaa gttgagcatt gctcaggctg
     3961 taagagatgg cctcctgccc gcaggcctgg gccagcagct gctggaggcc caggtggcat
     4021 ccggcttcct ggtcaatcca ctaaccaacc agagactgtc agtgcacgat gcagtgaagg
     4081 ctggcttggt gggcgtggag ctgagtgaac agctcaggca agtagagagg gctgtgacag
     4141 gatactcaga ccccttttca ggaggcctcc tttctctgtg gcaggccaag gaaaaggggc
     4201 ttgtatccca aagtgaggcc tttcctctcc tgcaagtcca gctggccaca gggggtgttg
     4261 tggaccctgt ccatagagtg cacctgcccc aggcagtggc ctacaagctt ggcctcctgg
     4321 atgagcagac aagccaggct ctgactgcca ctggggagga aaacaagctc ttttttgacc
     4381 ccaactcccg tgagaaggtg acataccagc agctcaagga actctgtgtg ctggatgctg
     4441 acactggcct gtggctgctg ccactgcccc aggacactgt gcttgaggtg gatgaccata
     4501 ctgcagtggc actgagagct atgaaggtgc ccatcaacat ggggaggttc caaggacata
     4561 gtgtgtcact ctgggacctg ctacactctg agtatgttgg agctgaaaag cggagggagc
     4621 tggtggcact ttgttgttcc gggagggctg ctgccctgcg acaggtcatc agtatggtca
     4681 ccaccctggt ggaggcggca gagaagcagc ccccacaggc caccttcaaa gggctccgga
     4741 agcaggtttc agcaggggac ttgtttaggt cccaactgat caccaagcag acactggatg
     4801 agctcaacca gggaaagagg acagtcgagg aagtgacaga gatggatagt gtaaggaggt
     4861 ccctggaagg aggcaacttc attgctgggg tccttattcg ggatacaaaa gagaagatga
     4921 gtatcccaga ggccttgaga aggcacattc tgaggccagg cacagccctg gtgctgctgg
     4981 aggcacaggc ggccaccggt ttcatcattg accccgtgga gaaccggaag ctaactgtag
     5041 agcaggcatt ccaggcaggg atgtttggta aggaaaccta cacgaagctg ttatcagctg
     5101 agcgtgccgt caccggctat acagacccct acacaggaga acagatctct ctctttcagg
     5161 ccatgcagag ggacctcatt gtcagggacc atggcatccg cctgctggaa gcccagattg
     5221 ctacaggtgg catcattgac cctgtacaca gccaccgtgt gccggtggat gtggcctacc
     5281 aacgtggcta cttcaatgag gagatgaacc gtgtgctggc tgacccaagt gatgatacca
     5341 agggcttctt cgaccccaac acccacgaga acctcacgta cctccagctg ctggagcgct
     5401 gcgtggagga ccccgagaca ggcctgtaca tgttggaaat tgtaaagaag ggagaaatct
     5461 acgtgtacat tgatgaggcc acaaggcaag tgctgagatc cagaactatg aaaatgcaca
     5521 tagggaagtt tgcaggccag acggtatccg tgtgggacct tttatcctcc cagtatttta
     5581 cagaaggaag gaaaagaaag cttttgcggg aatacagggc ccagaacata ggcctagaaa
     5641 aactgctgga ggttattacc tctactgtgg aagacacaga gaagcaaagc caaatattca
     5701 aagtaccggg cattcatggc gatgtgacgg ctgtggaact attcaactcc ggaatccttg
     5761 ataagaggac cctggacgcg ctccacagtg gggagagagg gcgccaggac ctctgccagc
     5821 tggagcatat aaaaatctac ctggaaggca ccaattgcat tgctggagtg acagcaccac
     5881 taacccagaa ggtgatgagc ttctacaagg ccagcaagga agagctcatc cccgcagggt
     5941 ttgcagccca gttactggaa gcacaggctg ccactgggta cttaatggac ccttgctccc
     6001 gccggaggtt gtgtgtggat gaggccatag cagctggcct ggtgggtgaa gacctgagag
     6061 agaggcttgt gagcgcagag aaggcagcga agggctatga agacccagcc acaggggaga
     6121 ctcttccact gttccaggcc atggagaaga agttagttgg gagggaggag gccctgaggc
     6181 tgctggaggt gcaggtggcc acgggtgggg tcattgaccc actacaccgc catcgtatcc
     6241 ctctggatgt agcctgtcag aggggatgtc tgtgtgaaga catcattgtg ctcattgctg
     6301 accaaaagca tatgaggaga aggttcgtgg atcccaacac acaagaaaag gtcacatacc
     6361 aggagctgca ggaccggtgc cggaaggagg agaagtcagg ctgggcactg ttcccagtgg
     6421 tcaaggacaa aaaagacatg gactatgttg atgaggccac caaaagggcc ctggaggcag
     6481 agcaggtgga agtgactgtg gggagataca gaggccagaa gccatccgtg tgggagctgc
     6541 tgaactcaga atatttttct gaggagaaga agattgaatt agttagaata tacaaagaag
     6601 acacagcccg ggcactgcag aaagttgtaa aacttattct aaaaatcatt gcagacaagg
     6661 aagggagaag caggcaactc tggttccgag gattacgaac tcaggtcaca gctgaggaac
     6721 tgctgagggc agcggtcatc accagaaaga ctctggagga cctggaggag ggcagagcca
     6781 cagtggatca gattgaagag acggaagatg tgaagagata cctgaagggc accagctgca
     6841 tagcgggtat cctggtgccc gtgcaggggg agcctggccg tcaggagaag atgagcatct
     6901 atcaggccat gtggaagggc ctgctgaggc ccggcacagc cctggtgctg ctggaggctc
     6961 aggcggccac cggcttcatc attgacccgg tgcacaaccg cagactgtct gtggaggagg
     7021 ccgtggctgc aggcgtggtg gggggagaga tccaggagaa gctgctggct gctgagcgtg
     7081 ctgtcactgg ctacacggac ccctacactg gggagcagat ctccctcttc caggccatgc
     7141 agagggacct cattgtgaaa gaccatggca ttcgcctcct ggaagcccag atcgccacag
     7201 gtggtgtcat tgaccccgtg catagccacc gtgttcccgt ggacgtggcc tacaaacgtg
     7261 gctactttga tgaggagatg aacagcattt tggcagaccc tggtgatgac accaagggct
     7321 tctttgaccc caacacgcac gagaacctca cgtatctgca gctgctcagg cgctgtgtgc
     7381 gggacccaga gaccgggttc tacatgctgc agctggcagg aaagggctcc agtgtgtacc
     7441 acgtgagcga ggacctgaga cgtgccctgc gagaggcccg tgtgagccca ggcacagtcg
     7501 acttccaggg ccagagcatc tctgtgtggg agcttctctt ctacagagag gtgcccgaga
     7561 gtctgcgcca ggacctgctg cgccggtacc aggcaggtgg actgagcgtt caggacgtga
     7621 gcaccacact cacctcactg ctggcccgaa ccaaggaggg gagcccacgt gctgatccgc
     7681 aaggaaccct ggggaaggcc accatggaag taaaacgggg tcatctccgg ggccatgagg
     7741 tcccagtgtg ggacgttctg acatccaact atgtgagcag ggacacccgg aaggagctgc
     7801 tggcccagtt cagctctggg gcactgactc tgcccatgct gaagaggaga ctgaccacca
     7861 ttattgaaga gaccgaggag acacaggagt cccaggcagg gcacagtgtc acctccctta
     7921 agcaacagga gtctgcggcc ggaagctcag gggcctacgc tgacaagggc gaagctgggg
     7981 acagcacaga cagtgccagg cagcaacagg agcagaccct gcgtgctgcc accatgcagg
     8041 tacgccgtgg gcagttccgg gaccagccag tttcagtgtg gaaagtcctg ttctcctcct
     8101 acctaagtga gacccgccgg gaggagctcc tgtcccagca cttggccggc aggctggggg
     8161 tcaatgaact aatttccatc ctcgtccaag tcatcgagga gacagaagag cggctcagca
     8221 gggtgtcttt accgggcctg aggcgccagg tgaccgtatc cgagttgggt aacactggca
     8281 tcctggaccc tgataccatg cgagaactgg cccagggcac caggaccata gatgaggtga
     8341 cagagatgga ctctgtcaag cgctacctga agggcaccag ctgcatagcg ggtatcctgg
     8401 tgcccgtgca gggggagcct ggccgtcagg agaagatgag catctatcag gccatgtgga
     8461 agggcctgct gaggcccggc acagccctgg tgctgctgga ggctcaggcg gccaccggct
     8521 tcatcattga cccggtgcac aaccgcagac tgtctgtgga ggaggccgtg gctgcaggcg
     8581 tggtgggggg agagatccag gagaagctgc tggctgctga gcgtgctgtc actggctaca
     8641 cggaccccta cactggggag cagatctccc tcttccaggc catgcagagg gacctcattg
     8701 tgaaagacca tggcattcgc ctcctggaag cccagatcgc cacaggtggt gtcattgacc
     8761 ccgtgcatag ccaccgtgtt cccgtggacg tggcctacaa acgtggctac tttgatgagg
     8821 agatgaacag cattttggca gaccctggtg atgacaccaa gggcttcttt gaccccaaca
     8881 cgcacgagaa cctcacgtat ctgcagctgc tcaggcgctg tgtgcgggac ccagagaccg
     8941 ggttctacat gctgcagctg gcaggaaagg gctccagtgt gtaccacgtg agcgaggacc
     9001 tgagacgtgc cctgcgagag gcccgtgtga gcccaggcac agtcgacttc cagggccaga
     9061 gcatctctgt gtgggagctt ctcttctaca gagaggtgcc cgagagtctg cgccaggacc
     9121 tgctgcgccg gtaccaggca ggtggactga gcgttcagga cgtgagcacc acactcacct
     9181 cactgctggc ccgaaccaag gaggggagcc cacgtgctga tccgcaagga accctgggga
     9241 aggccaccat ggaagtaaaa cggggtcatc tccggggcca tgaggtccca gtgtgggacg
     9301 ttctgacatc caactatgtg agcagggaca cccggaagga gctgctggcc cagttcagct
     9361 ctggggcact gactctgccc atgctgaaga ggagactgac caccattatt gaagagaccg
     9421 aggagacaca ggagtcccag gcagggcaca gtgtcacctc ccttaagcaa caggagtctg
     9481 cggccggaag ctcaggggcc tacgctgaca agggcgaagc tggggacagc acagacagtg
     9541 ccaggcagca acaggagcag accctgcgtg ctgccaccat gcaggtacgc cgtgggcagt
     9601 tccgggacca gccagtttca gtgtggaaag tcctgttctc ctcctaccta agtgagaccc
     9661 gccgggagga gctcctgtcc cagcacttgg ccggcaggct gggggtcaat gaactaattt
     9721 ccatcctcgt ccaagtcatc gaggagacag aagagcggct cagcagggtg tctttaccgg
     9781 gcctgaggcg ccaggtgacc gtatccgagt tgggtaacac tggcatcctg gaccctgata
     9841 ccatgcgaga actggcccag ggcaccagga ccatagatga ggtgacagag atggactctg
     9901 tcaagcgcta cctgaagggc accagctgca tagcgggtat cctggtgccc gtgcaggggg
     9961 agcctggccg tcaggagaag atgagcatct atcaggccat gtggaagggc ctgctgaggc
    10021 ccggcacagc cctggtgctg ctggaggctc aggcggccac cggcttcatc attgacccgg
    10081 tgcacaaccg cagactgtct gtggaggagg ccgtggctgc aggcgtggtg gggggagaga
    10141 tccaggagaa gctgctggct gctgagcgtg ctgtcactgg ctacacggac ccctacactg
    10201 gggagcagat ctccctcttc caggccatgc agagggacct cattgtgaaa gaccatggca
    10261 ttcgcctcct ggaagcccag atcgccacag gtggtgtcat tgaccccgtg catagccacc
    10321 gtgttcccgt ggacgtggcc tacaaacgtg gctactttga tgaggagatg aacagcattt
    10381 tggcagaccc tggtgatgac accaagggct tctttgaccc caacacgcac gagaacctca
    10441 cgtatctgca gctgctcagg cgctgtgtgc gggacccaga gaccgggttc tacatgctgc
    10501 agctggcagg aaagggctcc agtgtgtacc acgtgagcga ggacctgaga cgtgccctgc
    10561 gagaggcccg tgtgagccca ggcacagtcg acttccaggg ccagagcatc tctgtgtggg
    10621 agcttctctt ctacagagag gtgcccgaga gtctgcgcca ggacctgctg cgccggtacc
    10681 aggcaggtgg actgagcgtt caggacgtga gcaccacact cacctcactg ctggcccgaa
    10741 ccaaggaggg gagcccacgt gctgatccgc aaggaaccct ggggaaggcc accatggaag
    10801 taaaacgggg tcatctccgg ggccatgagg tcccagtgtg ggacgttctg acatccaact
    10861 atgtgagcag ggacacccgg aaggagctgc tggcccagtt cagctctggg gcactgactc
    10921 tgcccatgct gaagaggaga ctgaccacca ttattgaaga gaccgaggag acacaggagt
    10981 cccaggcagg gcacagtgtc acctccctta agcaacagga gtctgcggcc ggaagctcag
    11041 gggcctacgc tgacaagggc gaagctgggg acagcacaga cagtgccagg cagcaacagg
    11101 agcagaccct gcgtgctgcc accatgcagg tacgccgtgg gcagttccgg gaccagccag
    11161 tttcagtgtg gaaagtcctg ttctcctcct acctaagtga gacccgccgg gaggagctcc
    11221 tgtcccagca cttggccggc aggctggggg tcaatgaact aatttccatc ctcgtccaag
    11281 tcatcgagga gacagaagag cggctcagca gggtgtcttt accgggcctg aggcgccagg
    11341 tgaccgtatc cgagttgggt aacactggca tcctggaccc tgataccatg cgagaactgg
    11401 cccagggcac caggaccata gatgaggtga cagagatgga ctctgtcaag cgctacctga
    11461 agggcaccag ctgcatagcg ggtatcctgg tgcccgtgca gggggagcct ggccgtcagg
    11521 agaagatgag catctatcag gccatgtgga agggcctgct gaggcccggc acagccctgg
    11581 tgctgctgga ggctcaggcg gccaccggct tcatcattga cccggtgcac aaccgcagac
    11641 tgtctgtgga ggaggccgtg gctgcaggcg tggtgggggg agagatccag gagaagctgc
    11701 tggctgctga gcgtgctgtc actggctaca cggaccccta cactggggag cagatctccc
    11761 tcttccaggc catgcagagg gacctcattg tgaaagacca tggcattcgc ctcctggaag
    11821 cccagatcgc cacaggtggt gtcattgacc ccgtgcatag ccaccgtgtt cccgtggacg
    11881 tggcctacaa acgtggctac tttgatgagg agatgaacag cattttggca gaccctggtg
    11941 atgacaccaa gggcttcttt gaccccaaca cgcacgagaa cctcacgtat ctgcagctgc
    12001 tcaggcgctg tgtgcgggac ccagagaccg ggttctacat gctgcagctg gcaggaaagg
    12061 gctccagtgt gtaccacgtg agcgaggacc tgagacgtgc cctgcgagag gcccgtgtga
    12121 gcccaggcac agtcgacttc cagggccaga gcatctctgt gtgggagctt ctcttctaca
    12181 gagaggtgcc cgagagtctg cgccaggacc tgctgcgccg gtaccaggca ggtggactga
    12241 gcgttcagga cgtgagcacc acactcacct cactgctggc ccgaaccaag gaggggagcc
    12301 cacgtgctga tccgcaagga accctgggga aggccaccat ggaagtaaaa cggggtcatc
    12361 tccggggcca tgaggtccca gtgtgggacg ttctgacatc caactatgtg agcagggaca
    12421 cccggaagga gctgctggcc cagttcagct ctggggcact gactctgccc atgctgaaga
    12481 ggagactgac caccattatt gaagagaccg aggagacaca ggagtcccag gcagggcaca
    12541 gtgtcacctc ccttaagcaa caggagtctg cggccggaag ctcaggggcc tacgctgaca
    12601 agggcgaagc tggggacagc acagacagtg ccaggcagca acaggagcag accctgcgtg
    12661 ctgccaccat gcaggtacgc cgtgggcagt tccgggacca gccagtttca gtgtggaaag
    12721 tcctgttctc ctcctaccta agtgagaccc gccgggagga gctcctgtcc cagcacttgg
    12781 ccggcaggct gggggtcaat gaactaattt ccatcctcgt ccaagtcatc gaggagacag
    12841 aagagcggct cagcagggtg tctttccctg gcctgaggcg tcaggtgacc gcatccgagt
    12901 tgagtatcac tggcatcctg gaccctgata ccatgcgaga actggcccag ggcaccagga
    12961 ccatagatga ggtgacagag atggactctg tcaagcgcta cctgaagggc accagctgca
    13021 tagcgggtat cctggtgccc gtgcaggggg agcctggccg tcaggagaag atgagcatct
    13081 atcaggccat gtggaagggc ctgctgaggc ccggcacagc cctggtgctg ctggaggctc
    13141 aggcggccac cggcttcatc attgacccgg tgcacaaccg cagactgtct gtggaggagg
    13201 ccgtggctga aggcgtggta ggcggggaga tcagagagaa gctgctctct gctgagcgtg
    13261 ctgtcactgg gtacacggac ccctacactg gggagcagat ctccctcttc caggccatgc
    13321 agagggacct cattgtcaag aaccatggca ttcgcctcct ggaagcccag atcgccacag
    13381 gtggtgtcat tgaccccgtg catagccacc gtgttcccgt ggacgtggcc tacaaacgtg
    13441 gctactttga tgaggagatg aacagcattt tggcagaccc tggtgatgac accaagggct
    13501 tctttgaccc caacacacac gagaatctca cgtacctaca gcttttgcag agagctacag
    13561 ttgaaccaga gacaggattg ttgtttcttt ccctccttaa gggctgaaaa aaaaattttt
    13621 ttttgttatt cctgggactt aatggaaggt acctcactat tctcatagag cttgacctgt
    13681 atcattggtt tcttctaata ctctcatttc ttttttatta aggtgtaatt ttttaggtaa
    13741 tgctcttttc ttgctaccct tgtgcttgtg attggtactt ttgtgtccca tatattttct
    13801 ccctcctttt gtctatattg agtttttttt ggcaaccctc tataatttcc tcctgttttt
    13861 ccttatgact gttttctgtt gctgttttag ggagcatgac cttggagggt tttgggtttg
    13921 gtattttggt tccaagacaa agtttccctt ggctctcttc atactcactc tgttgaacag
    13981 tctggtgtca aactcagaga ttcacctgcc tctgcgtccc aaagactgac attaaagtct
    14041 tgtgccacca ctgcccagtc aaagaggtgg tcttttaatt gaggctttct ctgacagcat
    14101 ggcaatcgtt cgatatgctg tgcccactct tctcaaattg gcaatctttt ctattggttt
    14161 cccagtctct gggattgcac gtgtaaatca cccggcttgg acaaaacctt caacgttttt
    14221 aatatggcca cttttgtaca cggtatgtct tgatttgctg cctgtattta gtatcatgct
    14281 gttcatcttc tgtccatgta cctctgagac atctcggttc cgtgtatcac agtttttagt
    14341 gcacacatat cttttgataa taaatgaatg ttctgccctc a
//
