LOCUS       NM_001109457             731 bp    mRNA    linear   ROD 30-JUL-2024
DEFINITION  Rattus norvegicus epididymal peptidase inhibitor (Eppin), mRNA.
ACCESSION   NM_001109457 XM_001062609
VERSION     NM_001109457.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 731)
  AUTHORS   Stewart,M.J., Stewart,P. and Rivera-Posada,J.
  TITLE     De novo assembly of the transcriptome of Acanthaster planci testes
  JOURNAL   Mol Ecol Resour 15 (4), 953-966 (2015)
   PUBMED   25494938
REFERENCE   2  (bases 1 to 731)
  AUTHORS   Silva,E.J., Patrao,M.T., Tsuruta,J.K., O'Rand,M.G. and Avellar,M.C.
  TITLE     Epididymal protease inhibitor (EPPIN) is differentially expressed
            in the male rat reproductive tract and immunolocalized in maturing
            spermatozoa
  JOURNAL   Mol Reprod Dev 79 (12), 832-842 (2012)
   PUBMED   23070980
  REMARK    GeneRIF: data suggested that EPPIN expression in rats is more
            widespread than in humans and mice, and is androgen-dependent in
            the epididymis; findings provide new insights into its potential
            physiological roles in reproduction
REFERENCE   3  (bases 1 to 731)
  AUTHORS   O'Rand,M.G. and Widgren,E.E.
  TITLE     Loss of calcium in human spermatozoa via EPPIN, the semenogelin
            receptor
  JOURNAL   Biol Reprod 86 (2), 55 (2012)
   PUBMED   22075473
  REMARK    Publication Status: Online-Only
REFERENCE   4  (bases 1 to 731)
  AUTHORS   Rajesh,A., Madhubabu,G. and Yenugu,S.
  TITLE     Identification and characterization of Wfdc gene expression in the
            male reproductive tract of the rat
  JOURNAL   Mol Reprod Dev 78 (9), 633-641 (2011)
   PUBMED   21796715
REFERENCE   5  (bases 1 to 731)
  AUTHORS   McCrudden,M.T., Dafforn,T.R., Houston,D.F., Turkington,P.T. and
            Timson,D.J.
  TITLE     Functional domains of the human epididymal protease inhibitor,
            eppin
  JOURNAL   FEBS J 275 (8), 1742-1750 (2008)
   PUBMED   18331357
REFERENCE   6  (bases 1 to 731)
  AUTHORS   Wang,Z., Widgren,E.E., Richardson,R.T. and O'Rand,M.G.
  TITLE     Characterization of an eppin protein complex from human semen and
            spermatozoa
  JOURNAL   Biol Reprod 77 (3), 476-484 (2007)
   PUBMED   17567961
REFERENCE   7  (bases 1 to 731)
  AUTHORS   Sivashanmugam,P., Hall,S.H., Hamil,K.G., French,F.S., O'Rand,M.G.
            and Richardson,R.T.
  TITLE     Characterization of mouse Eppin and a gene cluster of similar
            protease inhibitors on mouse chromosome 2
  JOURNAL   Gene 312, 125-134 (2003)
   PUBMED   12909348
COMMENT     PROVISIONAL REFSEQ: This record has not yet been subject to final
            NCBI review. The reference sequence was derived from CH474005.2.
            
            On Oct 4, 2007 this sequence version replaced XM_001062609.1.
            
            ##Evidence-Data-START##
            RNAseq introns :: single sample supports all introns SAMEA5760383
                              [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
FEATURES             Location/Qualifiers
     source          1..731
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /db_xref="taxon:10116"
                     /chromosome="3"
                     /map="3q42"
     gene            1..731
                     /gene="Eppin"
                     /gene_synonym="Spinlw1; Wfdc8"
                     /note="epididymal peptidase inhibitor"
                     /db_xref="GeneID:685161"
                     /db_xref="RGD:1597722"
     exon            1..150
                     /gene="Eppin"
                     /gene_synonym="Spinlw1; Wfdc8"
                     /inference="alignment:Splign:2.1.0"
     CDS             60..464
                     /gene="Eppin"
                     /gene_synonym="Spinlw1; Wfdc8"
                     /note="serine protease inhibitor-like, with Kunitz and WAP
                     domains 1 (eppin); WAP four-disulfide core domain protein
                     8; serine peptidase inhibitor-like, with Kunitz and WAP
                     domains 1 (eppin); epididymal protease inhibitor; serine
                     protease inhibitor-like with Kunitz and WAP domains 1"
                     /codon_start=1
                     /product="eppin precursor"
                     /protein_id="NP_001102927.1"
                     /db_xref="GeneID:685161"
                     /db_xref="RGD:1597722"
                     /translation="MKFSRFVSILVLFGLLTKVQGPSLTDFLFPRRCPRFREECEHRE
                     RDLCTRDRDCQKREKCCIFSCGKKCLNPQQDICSLPKDSGYCMAYFPRWWYNKKNGTC
                     QLFIYGGCQGNNNNFQSQSICQNACEKKSNST"
     exon            151..282
                     /gene="Eppin"
                     /gene_synonym="Spinlw1; Wfdc8"
                     /inference="alignment:Splign:2.1.0"
     exon            283..450
                     /gene="Eppin"
                     /gene_synonym="Spinlw1; Wfdc8"
                     /inference="alignment:Splign:2.1.0"
     exon            451..731
                     /gene="Eppin"
                     /gene_synonym="Spinlw1; Wfdc8"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 tgaaggcagc agacgtttcc acattctaga acgaccatcc aggccagccc ctcgccagga
       61 tgaagttttc cagatttgtg agcatcctcg tgctatttgg cctgcttaca aaggtccagg
      121 gacctagtct aactgacttt ctgtttccca ggagatgccc cagattcaga gaggagtgtg
      181 aacacagaga gagggacctt tgtaccaggg acagggactg ccagaagaga gagaagtgct
      241 gtatcttcag ctgcggaaag aaatgtctaa acccccaaca agatatttgc agcctaccaa
      301 aagactctgg ctactgcatg gcttacttcc ctcgttggtg gtataataag aaaaacggca
      361 cgtgccaact cttcatctat ggtggttgcc agggaaacaa taacaacttc cagtcccaaa
      421 gtatatgcca gaatgcctgt gaaaaaaaaa gcaattccac ctgaacgggg gacccgctgg
      481 gaaaatcatc aggatttggc ttctgctcca agctctatgg acctgctgga tgctccaggc
      541 tggcgtctga gcactccaag gccttgggtc ttcccagtta ctggattcta ggatgcctcc
      601 ccgcccactc ggccagttcc tattcctaac tcctaccctt agctaccctg ctcagctcct
      661 ccgtcaaaag ctcccagttt gtttccatgt agctctcagc ttggttcagt aaagtatttg
      721 acttcgccct g
//
