LOCUS       NM_001305628            4414 bp    mRNA    linear   ROD 26-APR-2024
DEFINITION  Rattus norvegicus epsin 2 (Epn2), transcript variant 3, mRNA.
ACCESSION   NM_001305628 XM_006246522
VERSION     NM_001305628.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 4414)
  AUTHORS   Catsburg,L.A., Westra,M., van Schaik,A.M. and MacGillavry,H.D.
  TITLE     Dynamics and nanoscale organization of the postsynaptic endocytic
            zone at excitatory synapses
  JOURNAL   Elife 11, e74387 (2022)
   PUBMED   35072626
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4414)
  AUTHORS   Sakai,E., Miura,Y., Suzuki-Kouyama,E., Oka,K., Tachibana,M.,
            Kawabata,K., Sakurai,F. and Mizuguchi,H.
  TITLE     A mammalian mirtron miR-1224 promotes tube-formation of human
            primary endothelial cells by targeting anti-angiogenic factor
            epsin2
  JOURNAL   Sci Rep 7 (1), 5541 (2017)
   PUBMED   28717225
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 4414)
  AUTHORS   Guo,Z., Neilson,L.J., Zhong,H., Murray,P.S., Zanivan,S. and
            Zaidel-Bar,R.
  TITLE     E-cadherin interactome complexity and robustness resolved by
            quantitative proteomics
  JOURNAL   Sci Signal 7 (354), rs7 (2014)
   PUBMED   25468996
  REMARK    Publication Status: Online-Only
REFERENCE   4  (bases 1 to 4414)
  AUTHORS   Sen,A., Madhivanan,K., Mukherjee,D. and Aguilar,R.C.
  TITLE     The epsin protein family: coordinators of endocytosis and signaling
  JOURNAL   Biomol Concepts 3 (2), 117-126 (2012)
   PUBMED   22942912
REFERENCE   5  (bases 1 to 4414)
  AUTHORS   Chen,H., Ko,G., Zatti,A., Di Giacomo,G., Liu,L., Raiteri,E.,
            Perucco,E., Collesi,C., Min,W., Zeiss,C., De Camilli,P. and
            Cremona,O.
  TITLE     Embryonic arrest at midgestation and disruption of Notch signaling
            produced by the absence of both epsin 1 and epsin 2 in mice
  JOURNAL   Proc Natl Acad Sci U S A 106 (33), 13838-13843 (2009)
   PUBMED   19666558
REFERENCE   6  (bases 1 to 4414)
  AUTHORS   Hussain,N.K., Yamabhai,M., Bhakar,A.L., Metzler,M., Ferguson,S.S.,
            Hayden,M.R., McPherson,P.S. and Kay,B.K.
  TITLE     A role for epsin N-terminal homology/AP180 N-terminal homology
            (ENTH/ANTH) domains in tubulin binding
  JOURNAL   J Biol Chem 278 (31), 28823-28830 (2003)
   PUBMED   12750376
REFERENCE   7  (bases 1 to 4414)
  AUTHORS   Polo,S., Sigismund,S., Faretta,M., Guidi,M., Capua,M.R., Bossi,G.,
            Chen,H., De Camilli,P. and Di Fiore,P.P.
  TITLE     A single motif responsible for ubiquitin recognition and
            monoubiquitination in endocytic proteins
  JOURNAL   Nature 416 (6879), 451-455 (2002)
   PUBMED   11919637
REFERENCE   8  (bases 1 to 4414)
  AUTHORS   Rosenthal,J.A., Chen,H., Slepnev,V.I., Pellegrini,L., Salcini,A.E.,
            Di Fiore,P.P. and De Camilli,P.
  TITLE     The epsins define a family of proteins that interact with
            components of the clathrin coat and contain a new protein module
  JOURNAL   J Biol Chem 274 (48), 33959-33965 (1999)
   PUBMED   10567358
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from JAXUCZ010000010.1, CB583573.1
            and BC094524.1.
            
            On Mar 24, 2015 this sequence version replaced XM_006246522.2.
            
            Summary: The protein encoded by this gene is a member of the epsin
            protein family. Epsin proteins are endocytic adaptors that function
            in the formation of clatherin-coated vesicles. Epsins contain a
            highly conserved N-terminal homology domain that binds
            phosphatidylinositol 4,5-bisphosphate in the plasma membrane, two
            or three ubiquitin interacting motifs, two clathrin-binding motifs,
            a cluster of aspartate-proline-tryptophan/phenylalanine repeats,
            and two or three asparagine-proline-phenylalanine tripeptide
            repeats at the C-terminus. In mouse, simultaneous knockout of this
            gene and its paralog results in embryonic arrest due to disruption
            of Notch signaling, suggesting a role as a specialized endocytic
            adaptor. Alternative splicing results in multiple transcript
            variants. [provided by RefSeq, Mar 2015].
            
            Transcript Variant: This variant (3) represents the longest
            transcript and encodes the longer isoform (b). Variants 2 and 3
            encode the same isoform (b).
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data because no single transcript from the same
            strain was available for the full length of the gene. The extent of
            this transcript is supported by transcript alignments.
            
            ##Evidence-Data-START##
            Transcript exon combination :: FQ211574.1, SRR26360190.60415.1
                                           [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA5760383,
                                           SAMEA5760386 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-11                JAXUCZ010000010.1  46759082-46759092   c
            12-45               CB583573.1         1-34
            46-120              BC094524.1         1-75
            121-190             JAXUCZ010000010.1  46733561-46733630   c
            191-3130            BC094524.1         76-3015
            3131-4414           JAXUCZ010000010.1  46697238-46698521   c
FEATURES             Location/Qualifiers
     source          1..4414
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="BN"
                     /db_xref="taxon:10116"
                     /chromosome="10"
                     /map="10q22"
     gene            1..4414
                     /gene="Epn2"
                     /note="epsin 2"
                     /db_xref="GeneID:60443"
                     /db_xref="RGD:619773"
     exon            1..120
                     /gene="Epn2"
                     /inference="alignment:Splign:2.1.0"
     exon            121..190
                     /gene="Epn2"
                     /inference="alignment:Splign:2.1.0"
     exon            191..948
                     /gene="Epn2"
                     /inference="alignment:Splign:2.1.0"
     CDS             354..2276
                     /gene="Epn2"
                     /note="isoform b is encoded by transcript variant 3;
                     EPS-15-interacting protein 2; EH domain binding protein
                     epsin 2"
                     /codon_start=1
                     /product="epsin-2 isoform b"
                     /protein_id="NP_001292557.1"
                     /db_xref="GeneID:60443"
                     /db_xref="RGD:619773"
                     /translation="MTTSSIRRQMKNIVNNYSEAEIKVREATSNDPWGPSSSLMTEIA
                     DLTYNVVAFSEIMSMVWKRLNDHGKNWRHVYKALTLLDYLIKTGSERVAQQCRENIFA
                     IQTLKDFQYIDRDGKDQGINVREKSKQLVALLKDEERLKVERVQALKTKERMAQVATG
                     VGSNQITFGRGSSQPNLSTSYSEQEYGKAGGSPASYHGSPEASLCPQHRTGAMLGQSE
                     ELQPLSQRHPCLPHLGLASHPNGDWAQPCLTCDRAARATSPRVSSELEQARPQTSGEE
                     ELQLQLALAMSREVAEQEERLRRGDDLRLQMALEESRRDTVKVPKKKEVKACCKPGSH
                     SQQTTLLDLMDALPSSGPVAQKTEPWSTGTPANQTNPWGGTVAPANISDPWPSFGTKP
                     AASVDPWGVPTTASIQSVPKNSDPWAASQQPASDAGKTADAWGAAKPSPASGSFELFS
                     NFNGTVKDDFSEFDNLRTSKKPAESGASVPPQDSRTTSPDLFESQSLTSASSKPSSAR
                     KTPESFLGPNAALVNLDSLVTKPNPPAQSLNPFLAPGAAAPAPVNPFQVNQPQPLTLN
                     QLRGSPVLGSSASFGSGPGVETVAPMPSVAPHSALGATGSSLTPLGPTAMNMVGSVGI
                     PPSAAQPAGTTNPFLL"
     exon            949..1119
                     /gene="Epn2"
                     /inference="alignment:Splign:2.1.0"
     exon            1120..1232
                     /gene="Epn2"
                     /inference="alignment:Splign:2.1.0"
     exon            1233..1343
                     /gene="Epn2"
                     /inference="alignment:Splign:2.1.0"
     exon            1344..1518
                     /gene="Epn2"
                     /inference="alignment:Splign:2.1.0"
     exon            1519..1683
                     /gene="Epn2"
                     /inference="alignment:Splign:2.1.0"
     exon            1684..1770
                     /gene="Epn2"
                     /inference="alignment:Splign:2.1.0"
     exon            1771..1986
                     /gene="Epn2"
                     /inference="alignment:Splign:2.1.0"
     exon            1987..4414
                     /gene="Epn2"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 ctctcggccg ccatgttggc tggcgtgtgg gtgtcaaacg ctgcacgggc tcgggtcgct
       61 cccgcctctc cagcgtcgcc ggcggctgca gccttgcact cgcgctggcc gggaacagag
      121 tgctgctgaa gtggggcaca tagccagctg caggctcagg ggatgccagc caccaatgcc
      181 agcagtctgt ggcatggctg ccagcttctg ggacctgagg ctttggagga agaaatgact
      241 gtggatggcc tttttgttcc atctgcgttt ctgacacgga aacttcctag ggtgcaggct
      301 taggggacag gaaagtttct cttgaagggc ttcacatttg taacaagata aaaatgacaa
      361 cttcatctat tagacggcag atgaaaaaca ttgtgaacaa ttactcagag gctgaaatca
      421 aagtccggga agccacctcc aatgacccat ggggcccatc cagctctctg atgactgaga
      481 ttgctgacct gacctataat gtggtagcct tctcggagat catgagcatg gtttggaagc
      541 ggcttaatga ccatggcaag aactggcgac atgtatacaa ggcgctgaca ctgctggact
      601 accttatcaa gacaggttct gagcgggtgg cccagcagtg tcgggagaac atctttgcta
      661 tacagactct gaaggacttc cagtacattg accgtgatgg caaggaccag ggtattaatg
      721 ttcgagagaa gtcaaagcaa ctggttgctc tcctcaagga tgaggagcgg ctgaaggttg
      781 agagggttca ggctctcaaa accaaagagc gcatggctca ggtggccact ggtgtgggca
      841 gcaaccagat caccttcggt cgaggctcca gccagcccaa cctttctacc agctactcag
      901 agcaagagta tggcaaggct gggggctcgc cggcgtccta ccacggctcg ccagaggcct
      961 ctctgtgccc ccagcaccgc acaggggcca tgctgggtca gagtgaggag ctgcagccac
     1021 tgagccagcg ccacccctgc ctgccgcacc tgggcctagc ctcccaccca aatggcgact
     1081 gggcccagcc ctgcctcact tgtgaccgcg cagcccgagc tacttcccca cgagtgtcct
     1141 ctgagttgga gcaggcccgg ccacagacca gcggagaaga ggagctgcag ctgcaactgg
     1201 cacttgccat gagcagagag gttgcagaac aggaagaacg cctcaggcgg ggtgatgacc
     1261 tcaggttgca gatggctctg gaagaaagcc ggagagacac agtaaaagtt ccaaaaaaga
     1321 aagaggtgaa agcttgctgc aagccaggct cccactcgca gcagactacc ttgttggatt
     1381 taatggatgc cctccccagc tcaggccctg ttgcacagaa aactgagccg tggagtacgg
     1441 gaacccctgc caaccagacc aacccctggg gtggaaccgt ggcacctgcg aacatttctg
     1501 acccctggcc ttcatttggt accaagccag ctgcctctgt ggacccctgg ggagtaccta
     1561 ccacagccag catacagtct gtccccaaga actcagaccc ttgggcagcc tcacagcagc
     1621 ctgcctccga tgctggaaaa acagctgatg cctggggggc tgccaagcct agtcctgcct
     1681 cagggtcctt tgagctcttc agtaatttca acggtacagt taaagacgat ttttctgaat
     1741 tcgacaacct tcgaacttca aaaaaaccag ctgagtcagg ggcctcagta ccaccccagg
     1801 acagcagaac cacgagccct gacctctttg agtctcaatc cttgacttct gcctcgagca
     1861 agcctagcag tgctcggaaa acacctgagt ccttcctggg ccccaatgca gcactggtga
     1921 acctggactc actggtgact aagcctaatc caccagctca gtccctcaat cccttcctgg
     1981 caccaggtgc tgctgctcca gctcctgtca atccctttca ggtcaaccag ccccagccac
     2041 tgacactgaa ccagcttcgg ggaagccctg tcctgggaag cagtgcgtcc tttgggtctg
     2101 gtccaggggt ggagacggtg gctcccatgc cctctgtagc tccacactca gcactggggg
     2161 ccactggctc ctcattgaca ccactaggcc ctacagcaat gaacatggta ggcagtgtgg
     2221 gtattccccc atcagcagct cagccagcgg gcacaaccaa ccctttcctt ctctagtgcc
     2281 ccgtggggac ttgcctgagt gaacacccag agtgctgggc caaggacgtc aagtggggac
     2341 tcactctgat ttgtgtagtg gctgagaata tggtggtagg ggtattagag cttcaggtcc
     2401 agcttcctgg tgaaagggtg gttgttacaa tccagacttt agggtggcct cactgtccta
     2461 gtcagacccc tgggtgtctt cctgagtgct cagttcctct aaggtctgga aaggaggtat
     2521 ttatcagtgt tgctcaagat tctgggatgc tgtctagccc agaatttggg acagtcacat
     2581 tgtgtgtgtc ttgcccaccc cagttatctt cagggtgcgg acaaggtgaa gctctgactc
     2641 catttgacac tgaccgttgg gaagtggttg tgcatatttt attttccttt gactcaatgt
     2701 gagttcaagg taaacactac taccagtgtg gagctttaag ctgactggag cgcgaccctg
     2761 ccctatggct ctgtgcttgt gcacgtttgc acattttgtt gagtacagtt tcatatttga
     2821 gtttgcaaaa ttatctaata gtcttttttt ggctaatatt tttataacgt ggttcttatt
     2881 taactgtcta gttttgatag aattgaccaa gtctggctga attaaggtct tggcatgtat
     2941 tattttagtg gtctaatttc tcttatttat ggttttaact gtaagaagac tttaaaagaa
     3001 gagatatttg gatgacaaaa tatgccttat ggaaaactaa gcaaaattct ttatagcaaa
     3061 aaaaccggga atttgattat acaaaaagaa ttttattaaa acaagacaaa aacatccaac
     3121 aataacgaaa cccgtagcct ctagttgcct ttttcctttc ttcagctctt ttgatgaatt
     3181 accaaatcaa ttgacttcag cattttacct agatctgaga ggcttttttg ctactagtat
     3241 attgacatct taaggttaaa aggaaaaaca acgcatgatt gtgacttgcc agtttttatc
     3301 aactaatccc ttttttgtac ctgggatgca ggggttgcca gggacttctg tttactgcag
     3361 accagaagtg tggctggctg ccctgccatc atgctcaggt taatgagatt gtgtgaaaat
     3421 ggaggtgact ctacacttgt gggctcagag gtcaccacgg atggtccccc tagctgagtc
     3481 atgacaggga ctggccctga tttgtcttcc tgccagctcc aactcaggct actgaccagg
     3541 aagtaaccgt atcctatttt ggagaagaaa aagtcctcag gtggcataga tttaacatac
     3601 tgcactgata ctaccctttc tctcccaacc tcctgctgtc ctcactcctc accctcaagg
     3661 aaagtattaa atcttgaatt gcatttcttc aaaggaagga ctgcccctta ttggtcctgg
     3721 cagtttgtgt gtctttcctg caatatggaa ttttaacctt aagctccaag gacacccagc
     3781 cacctgccag ttcctctctg catccagaga aagttttgac cctcagcacc cacatagttg
     3841 actgagtgat tactgtttga gagaggactg cacttaggcc cctcctgagt ctgagtctac
     3901 atgctgttcc taggccccac aagccaaggg cccttcctaa ctaccctttc cacccaggac
     3961 ccaaggccta gggagaggct tgggctctgc tgttcaggaa ggctgagctg atggctgcct
     4021 gggagagatg gaggtatcac agggcaggga ggccaggcac cagtccagac tgcttccttc
     4081 agatggtata ggctagggcc agaactgcct gctgtgggcc cgtgattctg ctccagaaga
     4141 caagagccca tctgactgga tccccatccc agtagccatt gggctcttcc tcccacaggc
     4201 cacccagcgc atgtccctgt tacccgctgc actgatcatg aagccgctgg ccactgccat
     4261 gtgtgttgca cacgtgccac catgcccttc ttgatgaata ccctgaagga agtcaccccg
     4321 ctgcttctca gtcccagggc tggtgggtgg ttttggattt gtgttgactg ttgatacttt
     4381 actgtataaa tataatttat catttgtatc atga
//
