LOCUS       NM_057136               2540 bp    mRNA    linear   ROD 03-APR-2024
DEFINITION  Rattus norvegicus Epsin 1 (Epn1), mRNA.
ACCESSION   NM_057136 XM_039079399
VERSION     NM_057136.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 2540)
  AUTHORS   Brod,J., Hellwig,A. and Wieland,F.T.
  TITLE     Epsin but not AP-2 supports reconstitution of endocytic
            clathrin-coated vesicles
  JOURNAL   FEBS Lett 594 (14), 2227-2239 (2020)
   PUBMED   32337703
  REMARK    GeneRIF: Epsin but not AP-2 supports reconstitution of endocytic
            clathrin-coated vesicles.
REFERENCE   2  (bases 1 to 2540)
  AUTHORS   Sakai,E., Miura,Y., Suzuki-Kouyama,E., Oka,K., Tachibana,M.,
            Kawabata,K., Sakurai,F. and Mizuguchi,H.
  TITLE     A mammalian mirtron miR-1224 promotes tube-formation of human
            primary endothelial cells by targeting anti-angiogenic factor
            epsin2
  JOURNAL   Sci Rep 7 (1), 5541 (2017)
   PUBMED   28717225
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 2540)
  AUTHORS   Holkar,S.S., Kamerkar,S.C. and Pucadyil,T.J.
  TITLE     Spatial Control of Epsin-induced Clathrin Assembly by Membrane
            Curvature
  JOURNAL   J Biol Chem 290 (23), 14267-14276 (2015)
   PUBMED   25837255
  REMARK    GeneRIF: This study finds that epsin preferentially partitions to
            and assembles clathrin on highly curved membrane surfaces.
REFERENCE   4  (bases 1 to 2540)
  AUTHORS   Brooks,A., Shoup,D., Kustigian,L., Puchalla,J., Carr,C.M. and
            Rye,H.S.
  TITLE     Single particle fluorescence burst analysis of epsin induced
            membrane fission
  JOURNAL   PLoS One 10 (3), e0119563 (2015)
   PUBMED   25799353
  REMARK    Publication Status: Online-Only
REFERENCE   5  (bases 1 to 2540)
  AUTHORS   Wilhelm,B.G., Mandad,S., Truckenbrodt,S., Krohnert,K., Schafer,C.,
            Rammner,B., Koo,S.J., Classen,G.A., Krauss,M., Haucke,V., Urlaub,H.
            and Rizzoli,S.O.
  TITLE     Composition of isolated synaptic boutons reveals the amounts of
            vesicle trafficking proteins
  JOURNAL   Science 344 (6187), 1023-1028 (2014)
   PUBMED   24876496
REFERENCE   6  (bases 1 to 2540)
  AUTHORS   Rosenthal,J.A., Chen,H., Slepnev,V.I., Pellegrini,L., Salcini,A.E.,
            Di Fiore,P.P. and De Camilli,P.
  TITLE     The epsins define a family of proteins that interact with
            components of the clathrin coat and contain a new protein module
  JOURNAL   J Biol Chem 274 (48), 33959-33965 (1999)
   PUBMED   10567358
REFERENCE   7  (bases 1 to 2540)
  AUTHORS   Piros,E.T., Shen,L. and Huang,X.Y.
  TITLE     Purification of an EH domain-binding protein from rat brain that
            modulates the gating of the rat ether-a-go-go channel
  JOURNAL   J Biol Chem 274 (47), 33677-33683 (1999)
   PUBMED   10559257
REFERENCE   8  (bases 1 to 2540)
  AUTHORS   Traub,L.M., Downs,M.A., Westrich,J.L. and Fremont,D.H.
  TITLE     Crystal structure of the alpha appendage of AP-2 reveals a
            recruitment platform for clathrin-coat assembly
  JOURNAL   Proc Natl Acad Sci U S A 96 (16), 8907-8912 (1999)
   PUBMED   10430869
REFERENCE   9  (bases 1 to 2540)
  AUTHORS   Chen,H., Slepnev,V.I., Di Fiore,P.P. and De Camilli,P.
  TITLE     The interaction of epsin and Eps15 with the clathrin adaptor AP-2
            is inhibited by mitotic phosphorylation and enhanced by
            stimulation-dependent dephosphorylation in nerve terminals
  JOURNAL   J Biol Chem 274 (6), 3257-3260 (1999)
   PUBMED   9920862
REFERENCE   10 (bases 1 to 2540)
  AUTHORS   Chen,H., Fre,S., Slepnev,V.I., Capua,M.R., Takei,K., Butler,M.H.,
            Di Fiore,P.P. and De Camilli,P.
  TITLE     Epsin is an EH-domain-binding protein implicated in
            clathrin-mediated endocytosis
  JOURNAL   Nature 394 (6695), 793-797 (1998)
   PUBMED   9723620
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            JAXUCZ010000001.1.
            
            On or before Mar 3, 2021 this sequence version replaced
            XM_039079399.1, NM_057136.1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR22582416.86587.1,
                                           SRR23984936.75619.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA5760383,
                                           SAMEA5760386 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-203               JAXUCZ010000001.1  77789209-77789411   c
            204-307             JAXUCZ010000001.1  77787609-77787712   c
            308-637             JAXUCZ010000001.1  77785903-77786232   c
            638-887             JAXUCZ010000001.1  77780064-77780313   c
            888-1012            JAXUCZ010000001.1  77777129-77777253   c
            1013-1087           JAXUCZ010000001.1  77776734-77776808   c
            1088-1171           JAXUCZ010000001.1  77776089-77776172   c
            1172-1475           JAXUCZ010000001.1  77774271-77774574   c
            1476-1586           JAXUCZ010000001.1  77773795-77773905   c
            1587-1670           JAXUCZ010000001.1  77773578-77773661   c
            1671-1928           JAXUCZ010000001.1  77772114-77772371   c
            1929-2540           JAXUCZ010000001.1  77771335-77771946   c
FEATURES             Location/Qualifiers
     source          1..2540
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="BN"
                     /db_xref="taxon:10116"
                     /chromosome="1"
                     /map="1q12"
     gene            1..2540
                     /gene="Epn1"
                     /note="Epsin 1"
                     /db_xref="GeneID:117277"
                     /db_xref="RGD:619772"
     exon            1..203
                     /gene="Epn1"
                     /inference="alignment:Splign:2.1.0"
     exon            204..307
                     /gene="Epn1"
                     /inference="alignment:Splign:2.1.0"
     exon            308..637
                     /gene="Epn1"
                     /inference="alignment:Splign:2.1.0"
     CDS             410..2137
                     /gene="Epn1"
                     /note="EPS-15-interacting protein 1"
                     /codon_start=1
                     /product="epsin-1"
                     /protein_id="NP_476477.1"
                     /db_xref="GeneID:117277"
                     /db_xref="RGD:619772"
                     /translation="MSTSSLRRQMKNIVHNYSEAEIKVREATSNDPWGPSSSLMSEIA
                     DLTYNVVAFSEIMSMIWKRLNDHGKNWRHVYKAMTLMEYLIKTGSERVSQQCKENMYA
                     VQTLKDFQYVDRDGKDQGVNVREKAKQLVALLRDEDRLREERAHALKTKEKLAQTATA
                     SSAAVGSGPPPEAEQAWPQSSGEEELQLQLALAMSKEEADQPPSCGPEDDVQLQLALS
                     LSREEHDKEERIRRGDDLRLQMAIEESKRETGGKEESSLMDLADVFTTPAPPQASDPW
                     GGPASVPTAVPVAAAASDPWGAPAVPPAADPWGGAAPTPASGDPWRPAAPTGPSVDPW
                     GGTPAPAAGEGPTSDPWGSADGGAPVSGPPSSDPWAPAPAFSDPWGGSPAKPSSNGTA
                     VGGFDTEPDEFSDFDRLRTALPTSGSSTGELELLAGEVPARSPGAFDMSGVGGSLAES
                     VGSPPPAATPTPTPPTRKTPESFLGPNAALVDLDSLVSRPGPTPPGAKASNPFLPSGA
                     PATGPSVTNPFQPAPPATLTLNQLRLSPVPPVPGAPPTYISPLGGGPGLPPMMPPGPP
                     APNTNPFLL"
     exon            638..887
                     /gene="Epn1"
                     /inference="alignment:Splign:2.1.0"
     exon            888..1012
                     /gene="Epn1"
                     /inference="alignment:Splign:2.1.0"
     exon            1013..1087
                     /gene="Epn1"
                     /inference="alignment:Splign:2.1.0"
     exon            1088..1171
                     /gene="Epn1"
                     /inference="alignment:Splign:2.1.0"
     exon            1172..1475
                     /gene="Epn1"
                     /inference="alignment:Splign:2.1.0"
     exon            1476..1586
                     /gene="Epn1"
                     /inference="alignment:Splign:2.1.0"
     exon            1587..1670
                     /gene="Epn1"
                     /inference="alignment:Splign:2.1.0"
     exon            1671..1928
                     /gene="Epn1"
                     /inference="alignment:Splign:2.1.0"
     exon            1929..2540
                     /gene="Epn1"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 cccctccccc gcccggctct ccgcgcccct cccgggcgcc ggggcggcgg ggccgtcgcc
       61 gtgcggccct ccgtgcgtct gtgcgtgcgc acgtgcgcac ttctgcatcc cgcagcccgg
      121 cgcgcgcccg gcgacaccga ggccgagcgg gggcaggggt ctgaccgtca tgacccccgg
      181 gagcccggtg taagcgggcc gaggttggat catggtaggg ctcagtaaat ctccctccat
      241 ggcttgttgg agaggcatag actgttgatc ggagtctact ctggacccag gctgctccag
      301 ttgtgagatg tggtgatcta ccagtgtcca ttgcggtctc cctttgtgag tcgcgtcatc
      361 tctccatgcc ccggggggct ggtgcccctt gacaccacta ctgggcacaa tgtcgacatc
      421 atcgctgcgg cgtcagatga agaatatcgt ccacaactac tcagaggcag agatcaaggt
      481 ccgggaggcc acaagcaatg atccctgggg cccatccagc tccctcatgt cagagattgc
      541 tgacctcacc tacaatgttg tggccttctc cgagattatg agcatgatct ggaagcggct
      601 caacgaccat ggcaagaatt ggaggcacgt ctacaaggcc atgacgttga tggagtacct
      661 catcaagact ggctctgagc gtgtatcaca acagtgcaaa gagaacatgt atgctgtgca
      721 gacgctgaag gacttccaat atgtggaccg tgatggcaag gaccagggtg tgaatgtgcg
      781 ggagaaggcc aagcagctgg tggctttgct gcgtgatgag gatcggcttc gggaggagcg
      841 cgcccacgcg ctcaagacca aggagaaact ggcacagact gccacggctt cctcagcagc
      901 tgtgggctct ggaccacctc ccgaggcgga gcaggcatgg ccacagagca gcggggagga
      961 ggagttgcag ctccagctgg ccctggctat gagcaaggag gaggctgacc agcccccgtc
     1021 ctgcggcccc gaggatgacg tccagctcca gctggccctt agtttgagcc gagaggagca
     1081 cgataaggaa gagcggatcc gtcgtgggga tgacctgagg ctgcagatgg caatagagga
     1141 gagcaagagg gagactgggg gcaaggagga gtcatctctt atggatcttg ctgacgtctt
     1201 cacaacccca gcccctccac aggcctcaga cccttggggg ggcccggcat ctgtgcctac
     1261 tgctgtccct gtggctgctg ctgcctcaga cccttggggg gcacctgctg tccctccagc
     1321 tgctgatcct tggggtggtg cagcccccac gccagcctcc ggggatcctt ggaggcctgc
     1381 agcccctaca gggccctctg ttgacccttg gggtgggacc ccagctcctg cagctggaga
     1441 ggggcccaca tctgacccgt ggggaagcgc tgatggtggg gcccctgtca gtggaccccc
     1501 atcttctgac ccctgggcac ctgccccagc tttctcagac ccttggggag gttcacctgc
     1561 aaagcccagc agcaatggca ctgcagttgg gggctttgac actgagcccg atgagttctc
     1621 agacttcgac cgactccgca ctgccctgcc aacctctggg agcagcacag gggaattgga
     1681 gttgctcgca ggggaggttc ctgctcgcag ccccggggca ttcgacatga gtggggttgg
     1741 gggatctctg gctgagtctg tggggagtcc ccctcctgcg gcaaccccaa ctcctacccc
     1801 gccaacacgg aagactccgg agtcattcct aggccctaat gcagcccttg tggacttgga
     1861 ctcactggtg agccgaccag gccctacacc gccaggagcc aaggcttcca acccattcct
     1921 tccaagtgga gctccggcca ccggcccctc cgtcaccaat cctttccagc cagcaccccc
     1981 tgcaacgctc accctgaacc agctccgtct cagtcctgtg cccccagttc ctggagcgcc
     2041 acccacctac atctctcctc ttggtggagg ccctggcctg ccccccatga tgcccccggg
     2101 tcccccagcc cccaacacta accccttcct cctataatcc agggtaggag ggaggcctgg
     2161 ttcagctggc ttccccaatt tctgctccct gggagatcag tgttgtgagt gcatgtgaaa
     2221 tggggggggg ggcctccccc acccccagtg cccttccctc tcctggggcc cactcacact
     2281 acaacctctt cccaagtccc caacccacct ccccttgaga aaaactggac atggggatgg
     2341 ggaggggagc cggccagagg aggacccttt tctgtggcat tagatggggg agggacagct
     2401 ggggttcccc cacccatccc atctccctct aaactgacaa cccccaatca gtgtttgagc
     2461 ctcctcgttc ccttggcacc cctggtgaat ccttggtgat gattttggca acttcgggaa
     2521 taaatggcaa ttctcatgga
//
