LOCUS       NM_022936               1992 bp    mRNA    linear   ROD 04-DEC-2024
DEFINITION  Rattus norvegicus epoxide hydrolase 2 (Ephx2), mRNA.
ACCESSION   NM_022936
VERSION     NM_022936.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 1992)
  AUTHORS   Pu,Y., Cheng,R., Zhang,Q., Huang,T., Lu,C., Tang,Z., Zhong,Y.,
            Wu,L., Hammock,B.D., Hashimoto,K., Luo,Y. and Liu,Y.
  TITLE     Role of soluble epoxide hydrolase in the abnormal activation of
            fibroblast-like synoviocytes from patients with rheumatoid
            arthritis
  JOURNAL   Clin Immunol 257, 109850 (2023)
   PUBMED   38013165
  REMARK    GeneRIF: Role of soluble epoxide hydrolase in the abnormal
            activation of fibroblast-like synoviocytes from patients with
            rheumatoid arthritis.
REFERENCE   2  (bases 1 to 1992)
  AUTHORS   Zhou,Y., Wang,X.C., Wei,J.H., Xue,H.M., Sun,W.T., He,G.W. and
            Yang,Q.
  TITLE     Soluble epoxide hydrolase and TRPC3 channels jointly contribute to
            homocysteine-induced cardiac hypertrophy: Interrelation and
            regulation by C/EBPbeta
  JOURNAL   Biochim Biophys Acta Mol Basis Dis 1869 (4), 166643 (2023)
   PUBMED   36669577
  REMARK    GeneRIF: Soluble epoxide hydrolase and TRPC3 channels jointly
            contribute to homocysteine-induced cardiac hypertrophy:
            Interrelation and regulation by C/EBPbeta.
REFERENCE   3  (bases 1 to 1992)
  AUTHORS   Leuillier,M., Duflot,T., Menoret,S., Messaoudi,H., Djerada,Z.,
            Groussard,D., Denis,R.G.P., Chevalier,L., Karoui,A., Panthu,B.,
            Thiebaut,P.A., Schmitz-Afonso,I., Nobis,S., Campart,C., Henry,T.,
            Sautreuil,C., Luquet,S.H., Beseme,O., Feliu,C., Peyret,H.,
            Nicol,L., Henry,J.P., Renet,S., Mulder,P., Wan,D., Tesson,L.,
            Heslan,J.M., Duche,A., Jacques,S., Ziegler,F., Brunel,V.,
            Rautureau,G.J.P., Monteil,C., do Rego,J.L., do Rego,J.C.,
            Afonso,C., Hammock,B., Madec,A.M., Pinet,F., Richard,V., Anegon,I.,
            Guignabert,C., Morisseau,C. and Bellien,J.
  TITLE     CRISPR/Cas9-mediated inactivation of the phosphatase activity of
            soluble epoxide hydrolase prevents obesity and cardiac ischemic
            injury
  JOURNAL   J Adv Res 43, 163-174 (2023)
   PUBMED   36585106
  REMARK    GeneRIF: CRISPR/Cas9-mediated inactivation of the phosphatase
            activity of soluble epoxide hydrolase prevents obesity and cardiac
            ischemic injury.
REFERENCE   4  (bases 1 to 1992)
  AUTHORS   Matin,N., Fisher,C., Lansdell,T.A., Hammock,B.D., Yang,J.,
            Jackson,W.F. and Dorrance,A.M.
  TITLE     Soluble epoxide hydrolase inhibition improves cognitive function
            and parenchymal artery dilation in a hypertensive model of chronic
            cerebral hypoperfusion
  JOURNAL   Microcirculation 28 (1), e12653 (2021)
   PUBMED   32767848
  REMARK    GeneRIF: Soluble epoxide hydrolase inhibition improves cognitive
            function and parenchymal artery dilation in a hypertensive model of
            chronic cerebral hypoperfusion.
REFERENCE   5  (bases 1 to 1992)
  AUTHORS   Yeh,C.F., Chuang,T.Y., Hung,Y.W., Lan,M.Y., Tsai,C.H., Huang,H.X.
            and Lin,Y.Y.
  TITLE     Inhibition of soluble epoxide hydrolase regulates
            monocyte/macrophage polarization and improves neurological outcome
            in a rat model of ischemic stroke
  JOURNAL   Neuroreport 30 (8), 567-572 (2019)
   PUBMED   30950936
  REMARK    GeneRIF: Study shows that inhibition of soluble epoxide hydrolase
            regulates monocyte/macrophage polarization and improves
            neurological outcome in a rat model of ischemic stroke
REFERENCE   6  (bases 1 to 1992)
  AUTHORS   Traiser,M., Diener,B., Utesch,D. and Oesch,F.
  TITLE     The gap junctional intercellular communication is no prerequisite
            for the stabilization of xenobiotic metabolizing enzyme activities
            in primary rat liver parenchymal cells in vitro
  JOURNAL   In Vitro Cell Dev Biol Anim 31 (4), 266-273 (1995)
   PUBMED   7795845
REFERENCE   7  (bases 1 to 1992)
  AUTHORS   Knehr,M., Thomas,H., Arand,M., Gebel,T., Zeller,H.D. and Oesch,F.
  TITLE     Isolation and characterization of a cDNA encoding rat liver
            cytosolic epoxide hydrolase and its functional expression in
            Escherichia coli
  JOURNAL   J Biol Chem 268 (23), 17623-17627 (1993)
   PUBMED   8349641
REFERENCE   8  (bases 1 to 1992)
  AUTHORS   Beetham,J.K., Tian,T. and Hammock,B.D.
  TITLE     cDNA cloning and expression of a soluble epoxide hydrolase from
            human liver
  JOURNAL   Arch Biochem Biophys 305 (1), 197-201 (1993)
   PUBMED   8342951
REFERENCE   9  (bases 1 to 1992)
  AUTHORS   Arand,M., Knehr,M., Thomas,H., Zeller,H.D. and Oesch,F.
  TITLE     An impaired peroxisomal targeting sequence leading to an unusual
            bicompartmental distribution of cytosolic epoxide hydrolase
  JOURNAL   FEBS Lett 294 (1-2), 19-22 (1991)
   PUBMED   1743286
REFERENCE   10 (bases 1 to 1992)
  AUTHORS   Hollinshead,M. and Meijer,J.
  TITLE     Immunocytochemical analysis of soluble epoxide hydrolase and
            catalase in mouse and rat hepatocytes demonstrates a peroxisomal
            localization before and after clofibrate treatment
  JOURNAL   Eur J Cell Biol 46 (3), 394-402 (1988)
   PUBMED   3181162
COMMENT     PROVISIONAL REFSEQ: This record has not yet been subject to final
            NCBI review. The reference sequence was derived from X65083.1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: X65083.1, BC085732.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMD00132261,
                                           SAMD00132264 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
FEATURES             Location/Qualifiers
     source          1..1992
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="Sprague-Dawley"
                     /db_xref="taxon:10116"
                     /chromosome="15"
                     /map="15p12"
     gene            1..1992
                     /gene="Ephx2"
                     /note="epoxide hydrolase 2"
                     /db_xref="GeneID:65030"
                     /db_xref="RGD:620732"
     exon            1..142
                     /gene="Ephx2"
                     /inference="alignment:Splign:2.1.0"
     CDS             42..1706
                     /gene="Ephx2"
                     /EC_number="3.3.2.10"
                     /EC_number="3.1.3.76"
                     /note="CEH; SEH; epoxide hydratase; soluble epoxide
                     hydrolase; cytosolic epoxide hydrolase; epoxide hydrolase
                     2, cytoplasmic"
                     /codon_start=1
                     /product="bifunctional epoxide hydrolase 2"
                     /protein_id="NP_075225.1"
                     /db_xref="GeneID:65030"
                     /db_xref="RGD:620732"
                     /translation="MALRVAAFDLDGVLALPSIAGVLRHTEEALALPRDFLLGAFQMK
                     FPEGPTEQLMKGKITFSQWVPLMDESCRKSSKACGASLPENFSISEIFSQAMAARSIN
                     RPMLQAAAALKKKGFTTCIVTNNWLDDSDKRDILAQMMCELSQHFDFLIESCQVGMIK
                     PEPQIYKFVLDTLKAKPNEVVFLDDFGSNLKPARDMGMVTILVRDTASALRELEKVTG
                     TQFPEAPLPVPCSPNDVSHGYVTVKPGIRLHFVEMGSGPAICLCHGFPESWFSWRYQI
                     PALAQAGFRVLAIDMKGYGDSSSPPEIEEYAMELLCEEMVTFLNKLGIPQAVFIGHDW
                     AGVLVWNMALFHPERVRAVASLNTPLMPPNPEVSPMEVIRSIPVFNYQLYFQEPGVAE
                     AELEKNMSRTFKSFFRTSDDMGLLTVNKATEMGGILVGTPEDPKVSKITTEEEIEYYI
                     QQFKKSGFRGPLNWYRNTERNWKWSCKALGRKILVPALMVTAEKDIVLRPEMSKNMEN
                     WIPFLKRGHIEDCGHWTQIEKPAEVNQILIKWLKTEIQNPSVTSKI"
     exon            143..227
                     /gene="Ephx2"
                     /inference="alignment:Splign:2.1.0"
     exon            228..387
                     /gene="Ephx2"
                     /inference="alignment:Splign:2.1.0"
     exon            388..578
                     /gene="Ephx2"
                     /inference="alignment:Splign:2.1.0"
     exon            579..701
                     /gene="Ephx2"
                     /inference="alignment:Splign:2.1.0"
     exon            702..770
                     /gene="Ephx2"
                     /inference="alignment:Splign:2.1.0"
     exon            771..866
                     /gene="Ephx2"
                     /inference="alignment:Splign:2.1.0"
     exon            867..945
                     /gene="Ephx2"
                     /inference="alignment:Splign:2.1.0"
     exon            946..980
                     /gene="Ephx2"
                     /inference="alignment:Splign:2.1.0"
     exon            981..1007
                     /gene="Ephx2"
                     /inference="alignment:Splign:2.1.0"
     exon            1008..1093
                     /gene="Ephx2"
                     /inference="alignment:Splign:2.1.0"
     exon            1094..1205
                     /gene="Ephx2"
                     /inference="alignment:Splign:2.1.0"
     exon            1206..1280
                     /gene="Ephx2"
                     /inference="alignment:Splign:2.1.0"
     exon            1281..1314
                     /gene="Ephx2"
                     /inference="alignment:Splign:2.1.0"
     exon            1315..1417
                     /gene="Ephx2"
                     /inference="alignment:Splign:2.1.0"
     exon            1418..1487
                     /gene="Ephx2"
                     /inference="alignment:Splign:2.1.0"
     exon            1488..1568
                     /gene="Ephx2"
                     /inference="alignment:Splign:2.1.0"
     exon            1569..1627
                     /gene="Ephx2"
                     /inference="alignment:Splign:2.1.0"
     exon            1628..1992
                     /gene="Ephx2"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 cttcttgtct ttgtcagctt ggcgctgcag cccgggccat catggcgctg cgtgtggccg
       61 cgttcgacct tgacggagtg ctggccctcc cctctatagc cggggttctg cgccacaccg
      121 aggaggccct ggcgctgccc agagacttcc tacttggcgc tttccagatg aaattcccag
      181 agggacccac tgagcaactc atgaaaggaa agatcacatt ttcccagtgg gtaccactca
      241 tggatgaaag ctgcaggaag tcctccaaag cctgtggagc cagtctacct gagaatttct
      301 ccataagtga aatattcagc caagccatgg cagcaagaag catcaaccgc cccatgcttc
      361 aggcagctgc tgctctcaaa aagaaaggat tcacaacgtg cattgtcacc aacaactggc
      421 tggacgacag tgacaagaga gacatcctgg cccagatgat gtgtgagctg agccaacact
      481 ttgacttcct catagagtcc tgtcaggtcg ggatgatcaa gcctgagcct cagatctaca
      541 agtttgtact ggacaccctg aaggcaaaac ccaatgaggt tgttttccta gatgactttg
      601 gaagtaatct gaagccagcc cgtgacatgg ggatggttac catcctggtc cgcgacacag
      661 cctcggcttt gagagaactg gagaaagtca cagggacaca gtttcctgag gcacctctgc
      721 cagtcccgtg cagtccaaat gatgtcagcc atgggtatgt gacagtgaag ccagggatcc
      781 gtctgcactt tgtggagatg ggctctggcc ctgctatatg cctctgtcat gggtttcctg
      841 agagctggtt ttcttggagg taccagatcc ctgctctggc ccaggcgggc tttcgtgttc
      901 tagctataga catgaaaggc tatggagact catcttctcc tccagaaata gaagaatatg
      961 ctatggaatt gctgtgtgag gagatggtga cattcctgaa taaactggga atccctcaag
     1021 cagtgttcat tggccatgac tgggctggtg tgctggtgtg gaatatggct ctcttccacc
     1081 ctgagagagt gagggctgtg gccagtttga acactccatt aatgccacca aatcctgagg
     1141 tgtcccccat ggaagttatc agatcgatcc cagttttcaa ctatcagctg tactttcaag
     1201 agccaggagt ggctgaggct gaactggaaa agaacatgag tcggactttc aaaagcttct
     1261 tccgaaccag tgatgatatg ggtctcctca ctgtgaataa agccactgaa atggggggaa
     1321 tccttgtggg aactccagaa gatcccaagg tcagcaaaat tactactgag gaggaaatag
     1381 agtattacat acagcagttc aagaagtctg gcttcagagg ccctctaaac tggtatcgaa
     1441 acacagaaag aaactggaag tggagctgta aggcgttggg aaggaagatc ttggtccctg
     1501 ccctgatggt cacagctgag aaggacattg tactccgtcc tgaaatgtcc aagaacatgg
     1561 aaaactggat ccctttcctg aaaaggggac acatcgaaga ctgtggtcac tggacacaga
     1621 tagagaaacc ggcagaggtg aaccagattc tcatcaagtg gctgaagact gaaatccaga
     1681 acccatcggt gacctccaag atttagccag tggcgtgtcc tctgctgggg acacattttc
     1741 atttctggac gtggccttat ccacagccag cagcatcgtt cttttgccag cagtgatttt
     1801 ctttaaatga aaatgatcag atgtgatgta attttagatc aggaagaaag tggtgtgtct
     1861 gattcttttg aggatgactg tatcaccaaa ggagagatca caccccaata gggaggcatg
     1921 gggcagccca gtttgtacct ttgtagccaa acccaagcct gctctttctg aagcagctga
     1981 tcagagagta gg
//
