LOCUS       NM_012844               1758 bp    mRNA    linear   ROD 31-MAR-2024
DEFINITION  Rattus norvegicus epoxide hydrolase 1 (Ephx1), transcript variant
            2, mRNA.
ACCESSION   NM_012844 XM_341163
VERSION     NM_012844.4
KEYWORDS    RefSeq.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 1758)
  AUTHORS   Ding,Y., Li,Y., Zhang,X., He,J., Lu,D., Fang,X., Wang,Y., Wang,J.,
            Zhang,Y., Qiao,X., Gan,L.M., Chen,C. and Zhu,Y.
  TITLE     Soluble epoxide hydrolase activation by S-nitrosation contributes
            to cardiac ischemia-reperfusion injury
  JOURNAL   J Mol Cell Cardiol 110, 70-79 (2017)
   PUBMED   28736260
  REMARK    GeneRIF: These findings show the critical role of soluble epoxide
            hydrolase S-nitrosation in cardiac ischemia-reperfusion injury.
REFERENCE   2  (bases 1 to 1758)
  AUTHORS   Makhlouf,M.M. and Magdy,R.I.
  TITLE     The clinical relevance and prognostic significance of microsomal
            epoxide hydrolase gene polymorphisms and their susceptibility to
            acquired aplastic anemia: an Egyptian study
  JOURNAL   Biomarkers 21 (5), 416-423 (2016)
   PUBMED   26999617
REFERENCE   3  (bases 1 to 1758)
  AUTHORS   Nithipatikom,K., Endsley,M.P., Pfeiffer,A.W., Falck,J.R. and
            Campbell,W.B.
  TITLE     A novel activity of microsomal epoxide hydrolase: metabolism of the
            endocannabinoid 2-arachidonoylglycerol
  JOURNAL   J Lipid Res 55 (10), 2093-2102 (2014)
   PUBMED   24958911
REFERENCE   4  (bases 1 to 1758)
  AUTHORS   Martino,A., Campa,D., Jurczyszyn,A., Martinez-Lopez,J.,
            Moreno,M.J., Varkonyi,J., Dumontet,C., Garcia-Sanz,R.,
            Gemignani,F., Jamroziak,K., Stepiel,A., Jacobsen,S.E., Andersen,V.,
            Jurado,M., Landi,S., Rossi,A.M., Lesueur,F., Marques,H.,
            Dudzinski,M., Watek,M., Moreno,V., Orciuolo,E., Petrini,M.,
            Reis,R.M., Rios,R., Sainz,J., Vogel,U., Buda,G., Vangsted,A.J. and
            Canzian,F.
  TITLE     Genetic variants and multiple myeloma risk: IMMEnSE validation of
            the best reported associations--an extensive replication of the
            associations from the candidate gene era
  JOURNAL   Cancer Epidemiol Biomarkers Prev 23 (4), 670-674 (2014)
   PUBMED   24521996
REFERENCE   5  (bases 1 to 1758)
  AUTHORS   Khrunin,A.V., Khokhrin,D.V., Moisseev,A.A., Gorbunova,V.A. and
            Limborska,S.A.
  TITLE     Pharmacogenomic assessment of cisplatin-based chemotherapy outcomes
            in ovarian cancer
  JOURNAL   Pharmacogenomics 15 (3), 329-337 (2014)
   PUBMED   24533712
REFERENCE   6  (bases 1 to 1758)
  AUTHORS   Bell,P.A., Falany,C.N., McQuiddy,P. and Kasper,C.B.
  TITLE     Glucocorticoid repression and basal regulation of the epoxide
            hydrolase promoter
  JOURNAL   Arch Biochem Biophys 279 (2), 363-369 (1990)
   PUBMED   2350182
REFERENCE   7  (bases 1 to 1758)
  AUTHORS   Christou,M., Jovanovich,M.C. and Jefcoate,C.R.
  TITLE     Epoxide hydratase: sex specific expression and rate-limiting role
            in DMBA metabolism
  JOURNAL   Carcinogenesis 10 (10), 1883-1890 (1989)
   PUBMED   2507189
REFERENCE   8  (bases 1 to 1758)
  AUTHORS   Simmons,D.L. and Kasper,C.B.
  TITLE     Quantitation of mRNAs specific for the mixed-function oxidase
            system in rat liver and extrahepatic tissues during development
  JOURNAL   Arch Biochem Biophys 271 (1), 10-20 (1989)
   PUBMED   2469391
REFERENCE   9  (bases 1 to 1758)
  AUTHORS   Falany,C.N., McQuiddy,P. and Kasper,C.B.
  TITLE     Structure and organization of the microsomal xenobiotic epoxide
            hydrolase gene
  JOURNAL   J Biol Chem 262 (12), 5924-5930 (1987)
   PUBMED   3032949
  REMARK    Erratum:[J Biol Chem 1987 Jul 25;262(21):10412]
REFERENCE   10 (bases 1 to 1758)
  AUTHORS   Porter,T.D., Beck,T.W. and Kasper,C.B.
  TITLE     Complementary DNA and amino acid sequence of rat liver microsomal,
            xenobiotic epoxide hydrolase
  JOURNAL   Arch Biochem Biophys 248 (1), 121-129 (1986)
   PUBMED   3755318
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            JAXUCZ010000013.1.
            
            On Apr 11, 2023 this sequence version replaced NM_012844.3.
            
            Transcript Variant: This variant (2) contains an alternate 5'
            terminal non-coding exon compared to variant 1. Variants 1 and 2
            encode the same protein.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: M26125.1, SRR26643291.34570.1
                                           [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA5760383,
                                           SAMEA5760400 [ECO:0006172]
            ##Evidence-Data-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-167               JAXUCZ010000013.1  95262951-95263117   c
            168-355             JAXUCZ010000013.1  95259140-95259327   c
            356-536             JAXUCZ010000013.1  95257028-95257208   c
            537-764             JAXUCZ010000013.1  95252164-95252391   c
            765-894             JAXUCZ010000013.1  95251165-95251294   c
            895-1103            JAXUCZ010000013.1  95250478-95250686   c
            1104-1212           JAXUCZ010000013.1  95248443-95248551   c
            1213-1338           JAXUCZ010000013.1  95246925-95247050   c
            1339-1758           JAXUCZ010000013.1  95246079-95246498   c
FEATURES             Location/Qualifiers
     source          1..1758
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="BN"
                     /db_xref="taxon:10116"
                     /chromosome="13"
                     /map="13q26"
     gene            1..1758
                     /gene="Ephx1"
                     /gene_synonym="mEH; MEH8"
                     /note="epoxide hydrolase 1"
                     /db_xref="GeneID:25315"
                     /db_xref="RGD:2557"
     exon            1..167
                     /gene="Ephx1"
                     /gene_synonym="mEH; MEH8"
                     /inference="alignment:Splign:2.1.0"
     exon            168..355
                     /gene="Ephx1"
                     /gene_synonym="mEH; MEH8"
                     /inference="alignment:Splign:2.1.0"
     CDS             173..1540
                     /gene="Ephx1"
                     /gene_synonym="mEH; MEH8"
                     /EC_number="3.3.2.9"
                     /note="liver microsomal xenobiotic epoxide hydrolase;
                     epoxide hydrolase 1 (microsomal xenobiotic hydrolase);
                     epoxide hydratase; microsomal epoxide hydrolase; epoxide
                     hydrolase 1, microsomal (xenobiotic)"
                     /codon_start=1
                     /product="epoxide hydrolase 1"
                     /protein_id="NP_036976.2"
                     /db_xref="GeneID:25315"
                     /db_xref="RGD:2557"
                     /translation="MWLELVLASLLGFVIYWFVSRDKEETLPLGDGWWGPGSKPSAKE
                     DESIRPFKVETSDEEIKDLHQRIDRFRASPPLEGSRFHYGFNSNYMKKVVSYWRNEFD
                     WRKQVEILNQYPHFKTKIEGLDIHFIHVKPPQLPSGRTPKPLLMVHGWPGSFYEFYKI
                     IPLLTDPKSHGLSDEHVFEVICPSIPGYGYSEASSKKGLNSVATARIFYKLMTRLGFQ
                     KFYIQGGDWGSLICTNMAQMVPNHVKGLHLNMAFISRSFYTMTPLLGQRFGRFLGYTE
                     KDIELLYPYKEKVFYSIMRESGYLHIQATKPDTVGCALNDSPVGLAAYILEKFSTWTK
                     SEYRELEDGGLERKFSLDDLLVNIMIYWTTGTIVSSQRYYKENLGQGIMVHKHEGMKV
                     FVPTGFSAFPSELLHAPEKWVKVKYPKLISYSYMERGGHFAAFEEPKLLAQDIRKFVS
                     LAELQ"
     exon            356..536
                     /gene="Ephx1"
                     /gene_synonym="mEH; MEH8"
                     /inference="alignment:Splign:2.1.0"
     exon            537..764
                     /gene="Ephx1"
                     /gene_synonym="mEH; MEH8"
                     /inference="alignment:Splign:2.1.0"
     exon            765..894
                     /gene="Ephx1"
                     /gene_synonym="mEH; MEH8"
                     /inference="alignment:Splign:2.1.0"
     exon            895..1103
                     /gene="Ephx1"
                     /gene_synonym="mEH; MEH8"
                     /inference="alignment:Splign:2.1.0"
     exon            1104..1212
                     /gene="Ephx1"
                     /gene_synonym="mEH; MEH8"
                     /inference="alignment:Splign:2.1.0"
     exon            1213..1338
                     /gene="Ephx1"
                     /gene_synonym="mEH; MEH8"
                     /inference="alignment:Splign:2.1.0"
     exon            1339..1758
                     /gene="Ephx1"
                     /gene_synonym="mEH; MEH8"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 agccactctc acttcgtgct gtgaccaggg aggactcacg gagcctacac ttagccctgg
       61 taaacagcag agcatgctgg gataattctt cccagaaaag gaaaagcagg cacttctgtt
      121 cccagggaaa acaacaggag cactttggac ctccctgctg cagtcaggag tcatgtggct
      181 ggaacttgtc ctggcttccc ttctgggctt tgtcatctac tggtttgtct cccgggacaa
      241 ggaggaaacc ttaccactag gagatggatg gtgggggcca gggtcaaagc catcagccaa
      301 agaagatgag agcatccggc ccttcaaggt ggaaacatca gatgaggaga tcaaggactt
      361 acaccagagg atagataggt tccgggcatc cccacctttg gagggcagcc gcttccacta
      421 tggcttcaac tccaactaca tgaagaaagt ggtgtcctac tggaggaacg agtttgactg
      481 gaggaagcag gtggagatcc tcaaccagta ccctcacttc aagaccaaga tcgaagggct
      541 tgacatccac ttcatccatg tgaagcctcc ccagctgccc tcagggcgca ccccaaagcc
      601 cttgctgatg gtgcatggct ggcctggatc cttctatgag ttttacaaga tcatcccact
      661 actgactgac cccaagtccc acggtctgag tgacgagcac gtgtttgaag tcatctgtcc
      721 ctcgattcct ggctatggct actcagaggc atccagcaag aaaggtttaa attcggtggc
      781 cactgcgagg attttctaca agctgatgac acggctgggc ttccagaaat tctacattca
      841 aggcggggac tgggggtccc tcatctgcac caacatggcc cagatggttc ccaaccacgt
      901 gaaaggcctg cacttaaata tggctttcat ttcgagaagt ttttacacca tgactcctct
      961 cctgggccaa cgcttcggga gattccttgg ctacacagag aaggatatcg agctcttgta
     1021 cccctataag gagaaggttt tctacagcat catgagggag agtggctact tacacatcca
     1081 agccaccaag ccagacactg tgggctgtgc tctcaatgac tctcccgtgg gcctggctgc
     1141 ctacatctta gagaagttct ccacctggac caagtcagag taccgtgaac tggaggatgg
     1201 aggcctggag aggaagttct ccctggatga tctgctggtt aacatcatga tctactggac
     1261 gacaggaacc attgtctcct cccaacgcta ctacaaggag aatttgggcc agggcatcat
     1321 ggtccataaa catgagggga tgaaggtctt tgtgcccact ggcttttcag ccttcccttc
     1381 cgagctactg catgccccag aaaagtgggt gaaggtcaag taccccaaac tcatctccta
     1441 ttcctacatg gaacgtgggg gccactttgc tgcctttgaa gagcccaagc ttctggccca
     1501 ggacatccgc aagttcgtgt ccctggctga gctgcagtag tgacactgga taccaactgt
     1561 ggctttagca gcagccctgg ttcctcccaa gtcacactta tggaagatga cccctttctg
     1621 aggaataagt ttgttccctg accacactcg aggacccaga cttaaactcc acagagtcgt
     1681 atgttacccc catatgcttc acctcactac atagctgtgt tagctacatg gctttaatga
     1741 taaatggatt tatttcta
//
