LOCUS       NM_022251               3822 bp    mRNA    linear   ROD 02-JUN-2024
DEFINITION  Rattus norvegicus glutamyl aminopeptidase (Enpep), mRNA.
ACCESSION   NM_022251 XM_578082
VERSION     NM_022251.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 3822)
  AUTHORS   Berillo,O., Ouerd,S., Idris-Khodja,N., Rehman,A., Richer,C.,
            Sinnett,D., Kwitek,A.E., Paradis,P. and Schiffrin,E.L.
  TITLE     Chromosome 2 Fragment Substitutions in Dahl Salt-Sensitive Rats and
            RNA Sequencing Identified Enpep and Hs2st1 as Vascular Inflammatory
            Modulators
  JOURNAL   Hypertension 77 (1), 178-189 (2021)
   PUBMED   33161775
  REMARK    GeneRIF: Chromosome 2 Fragment Substitutions in Dahl Salt-Sensitive
            Rats and RNA Sequencing Identified Enpep and Hs2st1 as Vascular
            Inflammatory Modulators.
REFERENCE   2  (bases 1 to 3822)
  AUTHORS   Wangensteen,R., Segarra,A.B., Ramirez-Sanchez,M., Gasparo,M.D.,
            Dominguez,G., Banegas,I., Vargas,F., Vives,F. and Prieto,I.
  TITLE     Influence of thyroid disorders on the kidney expression and plasma
            activity of aminopeptidase A
  JOURNAL   Endocr Regul 49 (2), 68-72 (2015)
   PUBMED   25960007
  REMARK    GeneRIF: A discrepancy between the high expression of AP-A in
            kidney of hyperthyroid rats and the low activity of AP-A measured
            in plasma and kidney of hyperthyroid animals was found.
REFERENCE   3  (bases 1 to 3822)
  AUTHORS   Yang,Y., Liu,C., Lin,Y.L. and Li,F.
  TITLE     Structural insights into central hypertension regulation by human
            aminopeptidase A
  JOURNAL   J Biol Chem 288 (35), 25638-25645 (2013)
   PUBMED   23888046
REFERENCE   4  (bases 1 to 3822)
  AUTHORS   Ishii,M., Hattori,A., Numaguchi,Y., Ma,X., Nagasaka,T.,
            Tsujimoto,M., Murohara,T., Kobayashi,H. and Mizutani,S.
  TITLE     The effect of recombinant aminopeptidase A (APA) on hypertension in
            pregnant spontaneously hypertensive rats (SHRs)
  JOURNAL   Early Hum Dev 85 (9), 589-594 (2009)
   PUBMED   19608358
REFERENCE   5  (bases 1 to 3822)
  AUTHORS   Gonzales,P.A., Pisitkun,T., Hoffert,J.D., Tchapyjnikov,D.,
            Star,R.A., Kleta,R., Wang,N.S. and Knepper,M.A.
  TITLE     Large-scale proteomics and phosphoproteomics of urinary exosomes
  JOURNAL   J Am Soc Nephrol 20 (2), 363-379 (2009)
   PUBMED   19056867
REFERENCE   6  (bases 1 to 3822)
  AUTHORS   Troyanovskaya,M., Song,L., Jayaraman,G. and Healy,D.P.
  TITLE     Expression of aminopeptidase A, an angiotensinase, in glomerular
            mesangial cells
  JOURNAL   Hypertension 27 (3 Pt 2), 518-522 (1996)
   PUBMED   8613196
REFERENCE   7  (bases 1 to 3822)
  AUTHORS   Wenger,R.H., Kopf,M., Nitschke,L., Lamers,M.C., Kohler,G. and
            Nielsen,P.J.
  TITLE     B-cell maturation in chimaeric mice deficient for the heat stable
            antigen (HSA/mouse CD24)
  JOURNAL   Transgenic Res 4 (3), 173-183 (1995)
   PUBMED   7795661
REFERENCE   8  (bases 1 to 3822)
  AUTHORS   Song,L., Ye,M., Troyanovskaya,M., Wilk,E., Wilk,S. and Healy,D.P.
  TITLE     Rat kidney glutamyl aminopeptidase (aminopeptidase A): molecular
            identity and cellular localization
  JOURNAL   Am J Physiol 267 (4 Pt 2), F546-F557 (1994)
   PUBMED   7943354
REFERENCE   9  (bases 1 to 3822)
  AUTHORS   Li,L., Wang,J. and Cooper,M.D.
  TITLE     cDNA cloning and expression of human glutamyl aminopeptidase
            (aminopeptidase A)
  JOURNAL   Genomics 17 (3), 657-664 (1993)
   PUBMED   8244382
REFERENCE   10 (bases 1 to 3822)
  AUTHORS   Nanus,D.M., Engelstein,D., Gastl,G.A., Gluck,L., Vidal,M.J.,
            Morrison,M., Finstad,C.L., Bander,N.H. and Albino,A.P.
  TITLE     Molecular cloning of the human kidney differentiation antigen
            gp160: human aminopeptidase A
  JOURNAL   Proc Natl Acad Sci U S A 90 (15), 7069-7073 (1993)
   PUBMED   8346219
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AF146044.1, CK481363.1, BC066663.1, CB747518.1 and AF214568.1.
            
            On Apr 5, 2007 this sequence version replaced NM_022251.1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AF214568.1, BC066663.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA5756307,
                                           SAMEA5760383 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-160               AF146044.1         1-160
            161-178             CK481363.1         1-18
            179-1186            BC066663.1         1-1008
            1187-1338           CB747518.1         98-249
            1339-3618           BC066663.1         1161-3440
            3619-3822           AF214568.1         3436-3639
FEATURES             Location/Qualifiers
     source          1..3822
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="Sprague-Dawley"
                     /db_xref="taxon:10116"
                     /chromosome="2"
                     /map="2q42"
     gene            1..3822
                     /gene="Enpep"
                     /note="glutamyl aminopeptidase"
                     /db_xref="GeneID:64017"
                     /db_xref="RGD:621228"
     exon            1..1041
                     /gene="Enpep"
                     /inference="alignment:Splign:2.1.0"
     CDS             422..3259
                     /gene="Enpep"
                     /EC_number="3.4.11.7"
                     /note="EAP; AP-A; aminopeptidase A"
                     /codon_start=1
                     /product="glutamyl aminopeptidase"
                     /protein_id="NP_071587.2"
                     /db_xref="GeneID:64017"
                     /db_xref="RGD:621228"
                     /translation="MNFAEEEPSKKYCIKGKHVAIICATVVAVGLIVGLSVGLTRSCE
                     PGTTPAPSNPPPHTSTALPPQDQNVCPDSDDESGEWKNFRLPDFIQPVHYDLEVKVLM
                     EEDRYTGIVSISVNLSKDTRDLWLHIRETRITKLPELRRPSGEQVPIRRCFEYKKQEY
                     VVIQAEEDLAATSGDSVYRLTIEFEGWLNGSLVGFYRTTYTEDGQTKSIAATDHEPTD
                     ARKSFPCFDEPNKKATYNISLIHPKEYSALSNMPVEKEETLDNDWKKTTFMKSVPMST
                     YLVCFAVHQFTSIQRTSRSGKPLTVYVQPNQKQTAEYAANITKAVFDFFEDYFAMEYS
                     LPKLDKIAIPDFGTGAMENWGLVTYRETNLLYDPLLSASSNQQRVASVVAHELVHQWF
                     GNIVTMDWWDDLWLNEGFASFFEFLGVNHAEADWQMLSQVLLEDVLPVQEDDSLMSSH
                     PVVVTVSTPAEITSVFDGISYSKGASILRMLQDWITPEKFQKGCQIYLENFKFKNAKT
                     SDFWDSLEKASNQPVKEVMDTWTSQMGYPVVTVSGKQNVTQKRFLLDYKADPSQPPSA
                     LGYTWNIPIKWTENGNSNITVYYRSNREGITLNANLSGDGFLKINPDHIGFYRVNYEA
                     ETWDWIAETLSSNHMNFSSADRSSFIDDAFALARAQLLDYEKALNLTRYLTSEKDFLP
                     WERVISAVSYIISMFEDDRELYPLIETYFRSQVKPIADSLGWQDTGSHITKLLRASVL
                     GFACKMGAGEALGNASQLFEAWLKGNESIPVNLRLLVYRYGMQNSGNEAAWNYTLEQY
                     QKTSLAQEKEKLLYGLASVKDVTLLARYLEMLKDPNIIKTQDVFTVIRYISYNSYGKS
                     MAWNWIQLNWDYLVNRFTINDRYLGRIVTIAEPFNTELQLWQMQSFFAKYPNAGAGAK
                     PREQVLETVKNNIEWLKLNRKSISEWFTSMP"
     exon            1042..1183
                     /gene="Enpep"
                     /inference="alignment:Splign:2.1.0"
     exon            1184..1315
                     /gene="Enpep"
                     /inference="alignment:Splign:2.1.0"
     exon            1316..1436
                     /gene="Enpep"
                     /inference="alignment:Splign:2.1.0"
     exon            1437..1591
                     /gene="Enpep"
                     /inference="alignment:Splign:2.1.0"
     exon            1592..1705
                     /gene="Enpep"
                     /inference="alignment:Splign:2.1.0"
     exon            1706..1840
                     /gene="Enpep"
                     /inference="alignment:Splign:2.1.0"
     exon            1841..1906
                     /gene="Enpep"
                     /inference="alignment:Splign:2.1.0"
     exon            1907..1972
                     /gene="Enpep"
                     /inference="alignment:Splign:2.1.0"
     exon            1973..2124
                     /gene="Enpep"
                     /inference="alignment:Splign:2.1.0"
     exon            2125..2204
                     /gene="Enpep"
                     /inference="alignment:Splign:2.1.0"
     exon            2205..2338
                     /gene="Enpep"
                     /inference="alignment:Splign:2.1.0"
     exon            2339..2394
                     /gene="Enpep"
                     /inference="alignment:Splign:2.1.0"
     exon            2395..2545
                     /gene="Enpep"
                     /inference="alignment:Splign:2.1.0"
     exon            2546..2619
                     /gene="Enpep"
                     /inference="alignment:Splign:2.1.0"
     exon            2620..2724
                     /gene="Enpep"
                     /inference="alignment:Splign:2.1.0"
     exon            2725..2895
                     /gene="Enpep"
                     /inference="alignment:Splign:2.1.0"
     exon            2896..3036
                     /gene="Enpep"
                     /inference="alignment:Splign:2.1.0"
     exon            3037..3115
                     /gene="Enpep"
                     /inference="alignment:Splign:2.1.0"
     exon            3116..3822
                     /gene="Enpep"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 tcgaggctga cagaggtttg gcaaaatctc cacctctccc aacccccaca tccctgcccg
       61 cgttagccga taggctcaag ccctgtttta tatttaactc actccagttc aggaggggcg
      121 ggcctgggga gacgctgttt atacagagct gggccaacac tctcatccgt cctcctccgt
      181 caagtcagcc tctcttctga gccttcatcc cactgcttgg tttccgcatt catcccagag
      241 tggccggtga ggacgtgcag gactgggaag agacaccgga caccagacag agaggtgtgg
      301 gaaagcgaag tcaggctgcc aaactagggg acgccttcca attgaaaagg gaagtcagct
      361 gacgatagtt agttaaattt aacatccttt catgggtagt atctgacttc aggggagaaa
      421 aatgaacttt gcagaggaag agccctctaa gaaatactgc attaaaggca aacacgtggc
      481 catcatctgc gcaacggtgg tagcagtggg attaatagta ggactttctg tgggcttgac
      541 caggtcgtgt gagccgggca caacccccgc tccttccaac cctcctcccc acaccagcac
      601 tgccctgcct ccccaggatc agaatgtctg ccctgacagt gacgatgaaa gtggagaatg
      661 gaaaaacttc aggctgccgg acttcatcca gccagttcac tacgacctgg aggtgaaggt
      721 cctaatggag gaagacaggt acacgggcat agtgagcatc tctgtcaact tgagcaaaga
      781 cactcgtgac ctatggctgc acatcaggga aaccaggatc accaagctgc cggagctaag
      841 gaggccctcc ggggagcagg taccgattcg acggtgcttt gagtacaaga agcaggagta
      901 cgtggtgatt caggctgaag aagaccttgc ggccaccagc ggggacagtg tctatcggct
      961 gaccatagag tttgaaggct ggttgaacgg ctcccttgtg ggtttttaca gaaccaccta
     1021 cacggaggac gggcaaacca agagcatagc ggccactgac cacgaaccaa cagatgccag
     1081 gaagtccttc ccttgtttcg atgaacccaa caagaaggcg acttacaaca tatccctcat
     1141 tcacccgaaa gaatactcag cactttccaa tatgccagta gagaaggaag agacactgga
     1201 taacgactgg aagaaaacca ctttcatgaa gtccgtcccg atgagcactt acctggtgtg
     1261 ctttgctgta catcagttca cctcgataca gagaacatcc aggagtggca aaccactcac
     1321 tgtttacgtc cagcccaacc aaaagcaaac agcagagtat gcggcaaaca taaccaaagc
     1381 tgtgtttgac ttctttgaag actacttcgc tatggagtat tctcttccga aactggataa
     1441 aattgctatt ccagattttg gcaccggcgc catggaaaac tggggacttg tcacttaccg
     1501 agaaacaaac ctgctttatg accccctgct atcggcctca tccaaccagc agagagtagc
     1561 cagcgtggtt gcccacgagc tcgtgcacca gtggtttgga aatatcgtga ccatggactg
     1621 gtgggacgac ttgtggctaa atgaaggatt tgcttcgttc ttcgagttct tgggagtaaa
     1681 ccacgcagaa gcagactggc agatgctcag tcaggtgctg cttgaagacg tgctacctgt
     1741 gcaggaggat gactccctga tgtcctcaca tccggtggtt gtcacggtgt ctactccagc
     1801 tgaaataacg tctgtgttcg atggaatatc atacagcaag ggagcttcta tcctgagaat
     1861 gcttcaagac tggataacac cagagaaatt ccaaaagggg tgtcagattt acttggaaaa
     1921 cttcaagttc aagaacgcga aaacttccga cttttgggat tcactggaaa aggcaagtaa
     1981 tcaaccagtg aaagaagtaa tggacacctg gactagccag atgggctatc ctgtggttac
     2041 tgtgagtgga aagcagaacg tcacccagaa acgctttctg ttggactaca aagctgaccc
     2101 ttcccagcca ccgtcagcac ttggttacac atggaatatt ccaatcaaat ggaccgaaaa
     2161 tggcaattca aatatcacag tctactatag gtcaaacaga gaaggaatca ctttgaatgc
     2221 taatcttagc ggagatggtt ttcttaaaat aaacccagat cacattgggt tttatcgtgt
     2281 aaactatgaa gcagaaactt gggattggat agctgagact ctctcctcaa accacatgaa
     2341 cttctcttct gctgaccggt caagttttat cgatgatgct tttgctctgg caagagctca
     2401 acttctggat tatgagaagg ccctgaactt gaccaggtat ctcacatcag aaaaggattt
     2461 cttaccatgg gagagagtca tttcagctgt aagctacatc attagcatgt ttgaagatga
     2521 cagagagctg taccccctga tagagacata cttccgaagc caagtgaagc ccattgcgga
     2581 ttctctggga tggcaggata ccggaagcca catcacaaag ttactccggg cctctgtatt
     2641 aggattcgca tgcaagatgg gagccggaga agccttgggc aatgcttccc agttatttga
     2701 ggcctggctg aaagggaatg aaagcattcc tgtgaacctc aggctgctgg tgtatcgcta
     2761 tggaatgcag aactctggca atgaggcagc gtggaactat actctagagc agtaccagaa
     2821 aacatctcta gcacaagaga aagaaaagct gctctatggg ttagcttcgg tgaaggatgt
     2881 tacactcttg gcaaggtatc tggaaatgct caaagacccc aatattatta aaactcagga
     2941 cgtatttaca gtcatccgct acatctccta caacagctat gggaaaagca tggcctggaa
     3001 ctggatccaa ctcaactggg actatctggt caacagattt acaatcaacg acagatacct
     3061 tggccggatc gtcaccatag ccgagccctt caacactgag ctgcagctct ggcagatgca
     3121 gagcttcttt gcaaaatatc caaatgctgg cgctggagca aaacccagag agcaagtgct
     3181 ggaaacggtg aagaacaaca tcgagtggct aaaactgaac agaaagtcca tcagcgagtg
     3241 gttcaccagc atgccctagg agcgctgatg ccttccgttt gtgcctccgc tgcactgtgg
     3301 aacctgaggc gacctgtttt acagactgca gagggagcct tcaaaaccag ttctgctttt
     3361 gctaagtacc gtgtttatgt ctcgcaaagc tttttaaatt attcctcttt gtcgttgaag
     3421 gaagatacta atagagtatt taatatactc agggatttct gctaagtgta ctttataggg
     3481 atgtctttac aaactgaagg gtttaatagt cacattaatg tctttaaata tcattccttg
     3541 tatcttaaat ctgtgaaaat ggagcaattt ggtcccagct ttacattttc agtaccaaag
     3601 aaacaccact taatcttctc tcctgattga tttttaaagt ttaaatacca gtacgtgcag
     3661 gacgtattgc catcttaatc tttattttat tattcagaat tacatagacc taatatcatt
     3721 ttattcacaa acgtctcact gctttaaatc ctaccaaagt aaccttggct taagttttgc
     3781 tcaaggattg aatgaacgag ccaaaaaaaa aaaaaaaaaa aa
//
