LOCUS       NM_139325               2218 bp    mRNA    linear   ROD 29-MAR-2024
DEFINITION  Rattus norvegicus enolase 2 (Eno2), mRNA.
ACCESSION   NM_139325 XM_003750657
VERSION     NM_139325.4
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 2218)
  AUTHORS   Owusu,L., Xu,C., Chen,H., Liu,G., Zhang,G., Zhang,J., Tang,Z.,
            Sun,Z. and Yi,X.
  TITLE     Gamma-enolase predicts lung damage in severe acute
            pancreatitis-induced acute lung injury
  JOURNAL   J Mol Histol 49 (4), 347-356 (2018)
   PUBMED   29728894
  REMARK    GeneRIF: gamma-enolase may signal and correlate lung tissue damage
            well before obvious physical pathological tissue damage and might
            be a candidate diagnostic and/or prognostic marker.
REFERENCE   2  (bases 1 to 2218)
  AUTHORS   Shinohara,Y., Ohtani,T., Konno,A. and Hirai,H.
  TITLE     Viral Vector-Based Evaluation of Regulatory Regions in the
            Neuron-Specific Enolase (NSE) Promoter in Mouse Cerebellum In Vivo
  JOURNAL   Cerebellum 16 (5-6), 913-922 (2017)
   PUBMED   28508170
  REMARK    GeneRIF: Study analyzed the regions that regulate the promoter
            strength and cellular specificity of the 1.8 kb rNSE promoter in
            the cerebellum in vivo. The rNSE promoter was divided into three
            regions (A, B, and C). Our results showed that region C was
            indispensable for the promoter activity in mouse cerebellum and
            served as an interneuron-predominant basal promoter.
REFERENCE   3  (bases 1 to 2218)
  AUTHORS   Sawada,Y., Konno,A., Nagaoka,J. and Hirai,H.
  TITLE     Inflammation-induced reversible switch of the neuron-specific
            enolase promoter from Purkinje neurons to Bergmann glia
  JOURNAL   Sci Rep 6, 27758 (2016)
   PUBMED   27291422
  REMARK    GeneRIF: Study showed that the NSE promoter was activated in
            astrocytes, including Bergmann glia (BG), but not Purkinje cells
            (PCs), in the traumatic region of the cerebellar cortex. This PC to
            BG switch was associated with local microglial invasion.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 2218)
  AUTHORS   Hwang,P.T., Kwon,O.D., Kim,H.J., Kim,B.G., Kim,S.H., Jang,Y.W.,
            Kim,P.K., Han,G.Y. and Kim,C.W.
  TITLE     Hyperglycemia decreases the expression of ATP synthase beta subunit
            and enolase 2 in glomerular epithelial cells
  JOURNAL   Tohoku J Exp Med 231 (1), 45-56 (2013)
   PUBMED   24042457
  REMARK    GeneRIF: Hyperglycemia may impair mitochondrial function and alter
            glycolysis in glomerular epithelial cells by down-regulating the
            expression of ATP synthase beta subunit and enolase 2.
REFERENCE   5  (bases 1 to 2218)
  AUTHORS   Bohm,D., Keller,K., Pieter,J., Boehm,N., Wolters,D., Siggelkow,W.,
            Lebrecht,A., Schmidt,M., Kolbl,H., Pfeiffer,N. and Grus,F.H.
  TITLE     Comparison of tear protein levels in breast cancer patients and
            healthy controls using a de novo proteomic approach
  JOURNAL   Oncol Rep 28 (2), 429-438 (2012)
   PUBMED   22664934
REFERENCE   6  (bases 1 to 2218)
  AUTHORS   Sakimura,K., Kushiya,E., Takahashi,Y. and Suzuki,Y.
  TITLE     The structure and expression of neuron-specific enolase gene
  JOURNAL   Gene 60 (1), 103-113 (1987)
   PUBMED   2450052
REFERENCE   7  (bases 1 to 2218)
  AUTHORS   Sakimura,K., Kushiya,E., Obinata,M., Odani,S. and Takahashi,Y.
  TITLE     Molecular cloning and the nucleotide sequence of cDNA for
            neuron-specific enolase messenger RNA of rat brain
  JOURNAL   Proc Natl Acad Sci U S A 82 (21), 7453-7457 (1985)
   PUBMED   2865729
REFERENCE   8  (bases 1 to 2218)
  AUTHORS   Sakimura,K., Kushiya,E., Obinata,M. and Takahashi,Y.
  TITLE     Molecular cloning and the nucleotide sequence of cDNA to mRNA for
            non-neuronal enolase (alpha alpha enolase) of rat brain and liver
  JOURNAL   Nucleic Acids Res 13 (12), 4365-4378 (1985)
   PUBMED   2989793
REFERENCE   9  (bases 1 to 2218)
  AUTHORS   Marangos,P.J., Schmechel,D., Parma,A.M., Clark,R.L. and
            Goodwin,F.K.
  TITLE     Measurement of neuron-specific (NSE) and non-neuronal (NNE)
            isoenzymes of enolase in rat, monkey and human nervous tissue
  JOURNAL   J Neurochem 33 (1), 319-329 (1979)
   PUBMED   110910
REFERENCE   10 (bases 1 to 2218)
  AUTHORS   Fletcher,L., Rider,C.C. and Taylor,C.B.
  TITLE     Enolase isoenzymes. III. Chromatographic and immunological
            characteristics of rat brain enolase
  JOURNAL   Biochim Biophys Acta 452 (1), 245-252 (1976)
   PUBMED   990313
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            JAXUCZ010000004.1 and CB615520.1.
            
            On or before Jun 23, 2021 this sequence version replaced
            XM_003750657.3, NM_139325.3.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AF019973.1, M11931.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA5760433,
                                           SAMN12840105 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-6                 JAXUCZ010000004.1  159267215-159267220 c
            7-577               CB615520.1         1-571
            578-735             JAXUCZ010000004.1  159263728-159263885 c
            736-933             JAXUCZ010000004.1  159261706-159261903 c
            934-1135            JAXUCZ010000004.1  159261022-159261223 c
            1136-1244           JAXUCZ010000004.1  159260657-159260765 c
            1245-1303           JAXUCZ010000004.1  159259581-159259639 c
            1304-2218           JAXUCZ010000004.1  159258371-159259285 c
FEATURES             Location/Qualifiers
     source          1..2218
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="BN"
                     /db_xref="taxon:10116"
                     /chromosome="4"
                     /map="4q42"
     gene            1..2218
                     /gene="Eno2"
                     /gene_synonym="NSE; RNEN3"
                     /note="enolase 2"
                     /db_xref="GeneID:24334"
                     /db_xref="RGD:2554"
     exon            1..53
                     /gene="Eno2"
                     /gene_synonym="NSE; RNEN3"
                     /inference="alignment:Splign:2.1.0"
     exon            54..153
                     /gene="Eno2"
                     /gene_synonym="NSE; RNEN3"
                     /inference="alignment:Splign:2.1.0"
     CDS             69..1373
                     /gene="Eno2"
                     /gene_synonym="NSE; RNEN3"
                     /EC_number="4.2.1.11"
                     /note="neuron-specific enolase; neural enolase;
                     2-phospho-D-glycerate hydro-lyase; gamma-enolase-like;
                     enolase 2, gamma, neuronal"
                     /codon_start=1
                     /product="gamma-enolase"
                     /protein_id="NP_647541.1"
                     /db_xref="GeneID:24334"
                     /db_xref="RGD:2554"
                     /translation="MSIQKIWAREILDSRGNPTVEVDLHTAKGLFRAAVPSGASTGIY
                     EALELRDGDKQRYLGKGVLKAVDHINSTIAPALISSGLSVVEQEKLDNLMLELDGTEN
                     KSKFGANAILGVSLAVCKAGAAEKDLPLYRHIAQLAGNSDLILPVPAFNVINGGSHAG
                     NKLAMQEFMILPVGAESFRDAMRLGAEVYHTLKGVIKDKYGKDATNVGDEGGFAPNIL
                     ENSEALELVKEAIDKAGYTEKMVIGMDVAASEFYRDGKYDLDFKSPADPSRCITGDQL
                     GALYQDFVRNYPVVSIEDPFDQDDWAAWSKFTANVGIQIVGDDLTVTNPKRIERAVEE
                     KACNCLLLKVNQIGSVTEAIQACKLAQENGWGVMVSHRSGETEDTFIADLVVGLCTGQ
                     IKTGAPCRSERLAKYNQLMRIEEELGEEARFAGHNFRNPSVL"
     exon            154..249
                     /gene="Eno2"
                     /gene_synonym="NSE; RNEN3"
                     /inference="alignment:Splign:2.1.0"
     exon            250..308
                     /gene="Eno2"
                     /gene_synonym="NSE; RNEN3"
                     /inference="alignment:Splign:2.1.0"
     exon            309..378
                     /gene="Eno2"
                     /gene_synonym="NSE; RNEN3"
                     /inference="alignment:Splign:2.1.0"
     exon            379..512
                     /gene="Eno2"
                     /gene_synonym="NSE; RNEN3"
                     /inference="alignment:Splign:2.1.0"
     exon            513..735
                     /gene="Eno2"
                     /gene_synonym="NSE; RNEN3"
                     /inference="alignment:Splign:2.1.0"
     exon            736..933
                     /gene="Eno2"
                     /gene_synonym="NSE; RNEN3"
                     /inference="alignment:Splign:2.1.0"
     exon            934..1135
                     /gene="Eno2"
                     /gene_synonym="NSE; RNEN3"
                     /inference="alignment:Splign:2.1.0"
     exon            1136..1244
                     /gene="Eno2"
                     /gene_synonym="NSE; RNEN3"
                     /inference="alignment:Splign:2.1.0"
     exon            1245..1303
                     /gene="Eno2"
                     /gene_synonym="NSE; RNEN3"
                     /inference="alignment:Splign:2.1.0"
     exon            1304..2218
                     /gene="Eno2"
                     /gene_synonym="NSE; RNEN3"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 cgccgccgcc gtcaccaccg ccaccgccac cggctgagtc tgcagtcctc gaggagatcc
       61 cagccatcat gtctatacag aagatctggg cccgagagat cttggactcc cgtgggaatc
      121 ccaccgtgga ggtggatctc catactgcca aaggtctttt ccgggctgca gtccccagtg
      181 gggcctccac tggcatctat gaggccctgg agctaaggga tggggacaaa cagcgttact
      241 taggcaaagg tgtcctgaag gctgtggacc acatcaacag caccatcgca ccggccctca
      301 tcagctcagg cctctctgtg gtggagcagg agaagctgga caacctgatg ctggagttgg
      361 atgggactga gaacaaatcc aagtttgggg ccaatgccat cctgggtgtg tccctggccg
      421 tgtgcaaggc tggggcagcc gagaaggact tgcccctcta tcgccacatt gctcaactgg
      481 ctgggaactc cgacctcatc ctgcccgtgc cggcctttaa tgtgatcaac ggtggctctc
      541 atgctgggaa caagttggcc atgcaggagt tcatgatcct cccagtgggt gctgagagct
      601 ttcgggatgc catgcgactt ggggctgagg tgtaccacac actcaagggg gtcatcaagg
      661 acaagtacgg caaggatgcc actaatgtgg gggatgaagg cggctttgcc cccaatatcc
      721 tggagaacag cgaagctttg gagctggtga aggaagccat tgacaaggct ggctacacgg
      781 aaaagatggt gattggtatg gatgtggctg cctctgagtt ttaccgcgat ggcaaatacg
      841 acttggattt caagtctccc gctgaccctt cccgatgcat cactggggac cagcttgggg
      901 cactctacca ggactttgtc cggaactatc ctgtggtctc cattgaagac ccattcgacc
      961 aggatgactg ggcagcttgg tccaagttca cagccaatgt cggcatccag atagtgggtg
     1021 atgacctgac ggtgaccaac cccaagcgca tcgagcgggc agtggaggag aaggcctgca
     1081 actgtttgct gctcaaggtc aaccagatcg gctcagtcac agaagccatc caagcgtgca
     1141 agctggccca ggagaacggc tggggggtta tggtgagtca tcgctctgga gaaaccgagg
     1201 acacgttcat tgcagacctc gtagtgggac tgtgtacagg tcagatcaag actggtgccc
     1261 catgcagatc tgaacgtctg gcgaagtaca accagctcat gaggattgaa gaggagctgg
     1321 gggaggaggc tcgcttcgcg ggacacaact tccggaatcc cagtgtgctg tgaccccctg
     1381 cttgcctgaa caccggaaca tcatctcatt ctcctggagc ctctttcttg ctgtcccgac
     1441 ctgccatagt taccttgata ccttgagccc caagtcaccc agaacacctc gactcacctg
     1501 ctctggctgt tcttggcttc cacaacccct tgctgtctct actcttcctc ctctctgggc
     1561 cccatttttg gggggattcc agtctgccca ctttcccttc tattctctct aatcttaaaa
     1621 aaaaaaaaaa aatgacgact agaagaagcg ggtccacaga agaaccgcca gcgtccgaca
     1681 ggagcttcag gattggtgtg ttggggcgtt taaagtgggg ccacgtggca cgtgtgcttc
     1741 cctgccatcc atggtgtgtt aagccttgaa ctatgcacag agctggtgtt tggggagtgc
     1801 tggatgtgtg tgtgttcaca tttgtttgtt tgtttattta tttattcact tatttatttc
     1861 tcagcctgtc agtcgtctgc cattactcta cagtctgaaa gcatcagtat tttcacgtgg
     1921 ttccatttca agatgaccta ggatgggagg ttttgttagc atgggaaagg tcacagaaag
     1981 gttagcaatg gtttttcatt tggtgcacta actgaagctc ggtactttac agaatggggc
     2041 tgtgtacccg ggggactttt ctcctataac tctctcccca gccctaggtt cctcagtctt
     2101 tttctccggc tgcaccagag cgctgcctca ttcccccgtg ccatgtccca cagttgccac
     2161 tgtctctgtg gctttgaaat gaccaccact attaaagtct gaatcacagt gcacacca
//
