LOCUS       NP_001388306             803 aa            linear   ROD 13-FEB-2025
DEFINITION  protein enabled homolog isoform 1 [Rattus norvegicus].
ACCESSION   NP_001388306 XP_038946855
VERSION     NP_001388306.1
DBSOURCE    REFSEQ: accession NM_001401377.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (residues 1 to 803)
  AUTHORS   Deng,G., Luo,Y., Zhang,Y., Zhang,J. and He,Z.
  TITLE     Enabled homolog (ENAH) regulated by RNA binding protein splicing
            factor 3b subunit 4 (SF3B4) exacerbates the proliferation, invasion
            and migration of hepatocellular carcinoma cells via Notch signaling
            pathway
  JOURNAL   Bioengineered 13 (2), 2194-2206 (2022)
   PUBMED   35030977
REFERENCE   2  (residues 1 to 803)
  AUTHORS   Wang,D.D., Chen,Y.B., Zhao,J.J., Zhang,X.F., Zhu,G.C., Weng,D.S.,
            Pan,K., Lv,L., Pan,Q.Z., Jiang,S.S., Wang,L.L. and Xia,J.C.
  TITLE     TES functions as a Mena-dependent tumor suppressor in gastric
            cancer carcinogenesis and metastasis
  JOURNAL   Cancer Commun (Lond) 39 (1), 3 (2019)
   PUBMED   30728082
  REMARK    Publication Status: Online-Only
REFERENCE   3  (residues 1 to 803)
  AUTHORS   Riquelme,D.N., Meyer,A.S., Barzik,M., Keating,A. and Gertler,F.B.
  TITLE     Selectivity in subunit composition of Ena/VASP tetramers
  JOURNAL   Biosci Rep 35 (5), e00246 (2015)
   PUBMED   26221026
  REMARK    GeneRIF: The tetramerization domain (TD) at the C-termini of
            enabled/VASP proteins conferred the observed selectivity.
            Publication Status: Online-Only
REFERENCE   4  (residues 1 to 803)
  AUTHORS   Ram,R., Wescott,A.P., Varandas,K., Dirksen,R.T. and Blaxall,B.C.
  TITLE     Mena associates with Rac1 and modulates connexin 43 remodeling in
            cardiomyocytes
  JOURNAL   Am J Physiol Heart Circ Physiol 306 (1), H154-H159 (2014)
   PUBMED   24186093
  REMARK    GeneRIF: Mena is associated with active Rac1 in cardiomyocytes.
            Knockdown of Mena increased Rac1 activity, connexin43 expression
            and localization.
REFERENCE   5  (residues 1 to 803)
  AUTHORS   Kuo,J.C., Han,X., Hsiao,C.T., Yates,J.R. 3rd and Waterman,C.M.
  TITLE     Analysis of the myosin-II-responsive focal adhesion proteome
            reveals a role for beta-Pix in negative regulation of focal
            adhesion maturation
  JOURNAL   Nat Cell Biol 13 (4), 383-393 (2011)
   PUBMED   21423176
REFERENCE   6  (residues 1 to 803)
  AUTHORS   Giesemann,T., Schwarz,G., Nawrotzki,R., Berhorster,K.,
            Rothkegel,M., Schluter,K., Schrader,N., Schindelin,H., Mendel,R.R.,
            Kirsch,J. and Jockusch,B.M.
  TITLE     Complex formation between the postsynaptic scaffolding protein
            gephyrin, profilin, and Mena: a possible link to the microfilament
            system
  JOURNAL   J Neurosci 23 (23), 8330-8339 (2003)
   PUBMED   12967995
REFERENCE   7  (residues 1 to 803)
  AUTHORS   Tani,K., Sato,S., Sukezane,T., Kojima,H., Hirose,H., Hanafusa,H.
            and Shishido,T.
  TITLE     Abl interactor 1 promotes tyrosine 296 phosphorylation of mammalian
            enabled (Mena) by c-Abl kinase
  JOURNAL   J Biol Chem 278 (24), 21685-21692 (2003)
   PUBMED   12672821
REFERENCE   8  (residues 1 to 803)
  AUTHORS   Lanier,L.M., Gates,M.A., Witke,W., Menzies,A.S., Wehman,A.M.,
            Macklis,J.D., Kwiatkowski,D., Soriano,P. and Gertler,F.B.
  TITLE     Mena is required for neurulation and commissure formation
  JOURNAL   Neuron 22 (2), 313-325 (1999)
   PUBMED   10069337
REFERENCE   9  (residues 1 to 803)
  AUTHORS   Yamabhai,M. and Kay,B.K.
  TITLE     Examining the specificity of Src homology 3 domain--ligand
            interactions with alkaline phosphatase fusion proteins
  JOURNAL   Anal Biochem 247 (1), 143-151 (1997)
   PUBMED   9126384
REFERENCE   10 (residues 1 to 803)
  AUTHORS   Gertler,F.B., Niebuhr,K., Reinhard,M., Wehland,J. and Soriano,P.
  TITLE     Mena, a relative of VASP and Drosophila Enabled, is implicated in
            the control of microfilament dynamics
  JOURNAL   Cell 87 (2), 227-239 (1996)
   PUBMED   8861907
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            JAXUCZ010000013.1.
            
            On Feb 10, 2022 this sequence version replaced XP_038946855.1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            RNAseq introns :: mixed sample support SAMEA5760383, SAMEA5760386
                              [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            inferred exon combination :: based on alignments, homology
            RefSeq Select criteria    :: based on conservation
            ##RefSeq-Attributes-END##
FEATURES             Location/Qualifiers
     source          1..803
                     /organism="Rattus norvegicus"
                     /strain="BN"
                     /db_xref="taxon:10116"
                     /chromosome="13"
                     /map="13q26"
     Protein         1..803
                     /product="protein enabled homolog isoform 1"
                     /note="protein enabled homolog"
                     /calculated_mol_wt=86152
     Region          5..112
                     /region_name="EVH1_Ena_VASP-like"
                     /note="Enabled/VASP family EVH1 domain; cd01207"
                     /db_xref="CDD:269918"
     Site            order(16,23,69,71,77,79,81)
                     /site_type="other"
                     /note="proline-rich peptide binding site [polypeptide
                     binding]"
                     /db_xref="CDD:269918"
     Region          <260..536
                     /region_name="PHA03247"
                     /note="large tegument protein UL36; Provisional"
                     /db_xref="CDD:223021"
     Region          618..644
                     /region_name="WH2_hVASP-like"
                     /note="Wiskott-Aldrich syndrome protein (WASP)-homology
                     domain 2 (WH2) of human Vasodilator-stimulated
                     phosphoprotein and related proteins; cd22185"
                     /db_xref="CDD:409225"
     Site            order(625..626,628..629,632,634..638)
                     /site_type="other"
                     /note="actin-binding motif [polypeptide binding]"
                     /db_xref="CDD:409225"
     Region          634..637
                     /region_name="actin-binding sequence"
                     /note="actin-binding sequence [structural motif]"
                     /db_xref="CDD:409225"
     Region          765..799
                     /region_name="VASP_tetra"
                     /note="VASP tetramerization domain; pfam08776"
                     /db_xref="CDD:462599"
     CDS             1..803
                     /gene="Enah"
                     /coded_by="NM_001401377.1:397..2808"
                     /note="isoform 1 is encoded by transcript variant 1"
                     /db_xref="GeneID:360891"
                     /db_xref="RGD:1307666"
ORIGIN      
        1 mseqsicqar aavmvyddan kkwvpaggst gfsrvhiyhh tgnntfrvvg rkiqdhqvvi
       61 ncaipkglky nqatqtfhqw rdarqvygln fgskedanvf asammhalev lnsqeaaqsk
      121 vtatqdstnl rcifcgptlp rqnsqlpaqv qngpsqeeme iqrrqlqeqq rqkelererm
      181 ererlererl ererlererl eqeqlerqrq erehverler erlerlerer qdrerererl
      241 eqlereqvew ererrmsnaa pssdsslysa plpeysscqp psapppsyak visapvsdaa
      301 pdyavvtalp ptstpptppl rhsatrfats lgsafhpvlp hyatvprpln rnsrpsspvn
      361 tpssqppaak scawptsnfs plppsppimi ssppgkatgp rpvlpvcvss pvpqmppspt
      421 apngsldsvt ypvsppptsg paappppppp pppppppppp pplpplasls hcgsqasppp
      481 gtplastpss kpsvlpspsa aapasvetpl npelgdssas epglqaasqp aetpapqghv
      541 lgppappppp plpsgpayas alppppgppp ppplpsagpp pppppppplp nqvppppppp
      601 papplpasgi fsgsmsednr pltglaaaia gaklrkvsrv edgsfpsggn tvsvnlassk
      661 tdsgrgngpl plggsglmee msallarrrr iaekgstiet eqkedrseda epvtskaast
      721 stpeptrkpw ertntmngsk spvisrpkst pssqpsangv qtegldydrl kqdildemrk
      781 elaklkeeli dairqelsks nta
//
