LOCUS       NM_001398705            2678 bp    mRNA    linear   ROD 30-MAR-2024
DEFINITION  Rattus norvegicus engrailed homeobox 1 (En1), mRNA.
ACCESSION   NM_001398705 XM_001056699
VERSION     NM_001398705.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 2678)
  AUTHORS   Narytnyk,A., Verdon,B., Loughney,A., Sweeney,M., Clewes,O.,
            Taggart,M.J. and Sieber-Blum,M.
  TITLE     Differentiation of human epidermal neural crest stem cells
            (hEPI-NCSC) into virtually homogenous populations of dopaminergic
            neurons
  JOURNAL   Stem Cell Rev Rep 10 (2), 316-326 (2014)
   PUBMED   24399192
REFERENCE   2  (bases 1 to 2678)
  AUTHORS   Veenvliet,J.V., Dos Santos,M.T., Kouwenhoven,W.M., von Oerthel,L.,
            Lim,J.L., van der Linden,A.J., Koerkamp,M.J., Holstege,F.C. and
            Smidt,M.P.
  TITLE     Specification of dopaminergic subsets involves interplay of En1 and
            Pitx3
  JOURNAL   Development 140 (16), 3373-3384 (2013)
   PUBMED   23863478
  REMARK    Erratum:[Development. 2013 Oct;140(19):4116]
REFERENCE   3  (bases 1 to 2678)
  AUTHORS   Haubenberger,D., Reinthaler,E., Mueller,J.C., Pirker,W.,
            Katzenschlager,R., Froehlich,R., Bruecke,T., Daniel,G., Auff,E. and
            Zimprich,A.
  TITLE     Association of transcription factor polymorphisms PITX3 and EN1
            with Parkinson's disease
  JOURNAL   Neurobiol Aging 32 (2), 302-307 (2011)
   PUBMED   19345444
REFERENCE   4  (bases 1 to 2678)
  AUTHORS   Wang,Q., Cao,D.H., Jin,C.L., Lin,C.K., Ma,H.W. and Wu,Y.Y.
  TITLE     A method of utrophin up-regulation through RNAi-mediated knockdown
            of the transcription factor EN1
  JOURNAL   J Int Med Res 39 (1), 161-171 (2011)
   PUBMED   21672318
REFERENCE   5  (bases 1 to 2678)
  AUTHORS   Cao,D.H., Wang,Q., Lin,C.K., Wang,Z.D., Zhang,X. and Jin,C.L.
  TITLE     [The functions of GLI3 and EN1 genes in idiopathic congenital
            talipes equinovarus]
  JOURNAL   Yi Chuan 31 (12), 1214-1220 (2009)
   PUBMED   20042388
  REMARK    GeneRIF: The low expression level of EN1 contribute to the
            up-regulation of GLI3 in idiopathic congenital talipes equinovarus.
REFERENCE   6  (bases 1 to 2678)
  AUTHORS   Joliot,A., Trembleau,A., Raposo,G., Calvet,S., Volovitch,M. and
            Prochiantz,A.
  TITLE     Association of Engrailed homeoproteins with vesicles presenting
            caveolae-like properties
  JOURNAL   Development 124 (10), 1865-1875 (1997)
   PUBMED   9169834
REFERENCE   7  (bases 1 to 2678)
  AUTHORS   Kury,P., Schaeren-Wiemers,N. and Monard,D.
  TITLE     Protease nexin-1 is expressed at the mouse met-/mesencephalic
            junction and FGF signaling regulates its promoter activity in
            primary met-/mesencephalic cells
  JOURNAL   Development 124 (6), 1251-1262 (1997)
   PUBMED   9102311
REFERENCE   8  (bases 1 to 2678)
  AUTHORS   Danielian,P.S. and McMahon,A.P.
  TITLE     Engrailed-1 as a target of the Wnt-1 signalling pathway in
            vertebrate midbrain development
  JOURNAL   Nature 383 (6598), 332-334 (1996)
   PUBMED   8848044
REFERENCE   9  (bases 1 to 2678)
  AUTHORS   Loomis,C.A., Harris,E., Michaud,J., Wurst,W., Hanks,M. and
            Joyner,A.L.
  TITLE     The mouse Engrailed-1 gene and ventral limb patterning
  JOURNAL   Nature 382 (6589), 360-363 (1996)
   PUBMED   8684466
REFERENCE   10 (bases 1 to 2678)
  AUTHORS   Hanks,M., Wurst,W., Anson-Cartwright,L., Auerbach,A.B. and
            Joyner,A.L.
  TITLE     Rescue of the En-1 mutant phenotype by replacement of En-1 with
            En-2
  JOURNAL   Science 269 (5224), 679-682 (1995)
   PUBMED   7624797
COMMENT     INFERRED REFSEQ: This record is predicted by genome sequence
            analysis and is not yet supported by experimental evidence. The
            reference sequence was derived from JAXUCZ010000013.1.
            
            On Dec 21, 2021 this sequence version replaced XM_001056699.7.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR21887623.169094.1,
                                           SRR26360181.1565222.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA5760383, SAMEA5760386
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-1566              JAXUCZ010000013.1  34303684-34305249
            1567-2678           JAXUCZ010000013.1  34308158-34309269
FEATURES             Location/Qualifiers
     source          1..2678
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="BN"
                     /db_xref="taxon:10116"
                     /chromosome="13"
                     /map="13q11"
     gene            1..2678
                     /gene="En1"
                     /note="engrailed homeobox 1"
                     /db_xref="GeneID:685360"
                     /db_xref="RGD:1595088"
     exon            1..1566
                     /gene="En1"
                     /inference="alignment:Splign:2.1.0"
     CDS             654..1883
                     /gene="En1"
                     /note="engrailed 1"
                     /codon_start=1
                     /product="homeobox protein engrailed-1"
                     /protein_id="NP_001385634.1"
                     /db_xref="GeneID:685360"
                     /db_xref="RGD:1595088"
                     /translation="MEEQQPEPKSQRDSGLGAVAAAAAVVAAAAPGGLSLSLSPGASG
                     SSGSDGDSVPVSPQPAPPSPPAAPCLPPLAHHPHLPPHPPPPPPPPPPPPPPQHLAAP
                     AHQPQPAAQLHRTTNFFIDNILRPDFGCKKEQPLPQLLVASAAAGGGAAGGGSRVERD
                     RSQTGAGRDPVHSLGTRASGAASLLCAPDANCGPPDGSQPATAVGAGASKAGNPAAAA
                     AAAAAAAAAAVAAAAAAASKPSDSGGGSGGSAGSPGAQGAKFPEHNPAILLMGSANGG
                     PVVKTDSQQPLVWPAWVYCTRYSDRPSSGPRTRKLKKKKNEKEDKRPRTAFTAEQLQR
                     LKAEFQANRYITEQRRQTLAQELSLNESQIKIWFQNKRAKIKKATGIKNGLALHLMAQ
                     GLYNHSTTTVQDKDESE"
     exon            1567..2678
                     /gene="En1"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 gatttttttt ttttttttaa gcgagagagt ttgtgcaaag atccgagctg tcagagattt
       61 caggggggaa aacggagccc ggcactggcg gagacgcgct ctccctgcaa aaaagcaaag
      121 gcgattaaag gcgctgccag cctcgcgctc tgggcacagc tgagcgtgac actggggaag
      181 tcaaacccct ctttacagcc taggaagatg gctagacttt aaagactatt ttttcccttt
      241 aaggaaaaaa tctcggagct taaaaaaatc atttttttct tttctttttt tcctcctttt
      301 atttttttaa gccgaggctt actcccccat taagaccaat cactgaaatc tggttgctga
      361 aagaaaaaaa gaaaaagaaa aggaaaaaat agccaagtgt cttcactgta tctggatgtc
      421 tacaaattag agagagggag agagcgagat ttgctccacc agagcgggcg agagccaggc
      481 cagacgctcg gctttctttt ttccttctgt acccgccctg tgccttcgct gaggcttcgc
      541 cttgcctcct tcctctccgc gcacccccac gggcccgctg gcaaagtggg gtggggagcg
      601 aggcgcgggg ggcgggggcc ggcgccgcgg ccagggctgc cgggcggccg agcatggaag
      661 aacagcagcc ggagcctaaa agtcagcgcg actcgggcct cggcgcggtg gcagcggcgg
      721 cggcggtggt ggcggcggcg gccccgggcg gcctcagcct gagtctcagc ccaggagcca
      781 gcggcagcag cggcagcgat ggagacagcg tgccggtgtc cccgcagcca gcgcccccgt
      841 cgcctcctgc ggcaccctgc ctgccgcccc tggcccatca cccgcacctc cccccgcatc
      901 ccccgccccc gccgccaccg ccgccgccgc cgccgccacc gcagcatctc gcggcgcctg
      961 ctcaccagcc gcagcccgcg gcccagctgc accgcaccac caactttttc atcgataaca
     1021 ttctaaggcc cgattttggt tgcaaaaagg aacagcccct gccgcagctc ctagtggctt
     1081 cggctgcagc cggaggaggt gcagcaggag gtggaagccg cgtggagcgt gaccgaagcc
     1141 agactggtgc aggtagagac cctgtccact ctctgggcac acgagcttcg ggcgctgcct
     1201 cgctcttgtg tgctccagat gcgaactgtg gcccacccga cggctcccag cccgccaccg
     1261 ccgtcggcgc gggcgcatcc aaagcgggga acccggctgc tgcggcggct gcagccgcgg
     1321 cagcggctgc agcggcagtg gcagcggcgg cggccgcagc atcgaagccc tcggacagtg
     1381 gcggtggcag tggaggcagc gcaggaagtc ctggggcgca gggtgccaag ttcccggaac
     1441 acaaccccgc gatcctactc atgggttcgg ctaacggtgg gccggtagtc aagactgact
     1501 cgcagcaacc cctagtctgg cccgcctggg tctactgcac acgctactcg gaccgtccat
     1561 cctctggtcc acgcaccagg aagctaaaga agaaaaagaa tgagaaggaa gacaagcggc
     1621 cgcggacggc gttcacggcc gagcagctgc agagactcaa ggcggagttc caggcaaacc
     1681 gctacatcac ggagcagcgg cgacagaccc tggcccagga gctcagcctg aacgagtccc
     1741 agatcaagat ctggttccaa aacaagcgtg ccaagatcaa gaaagccacc ggcatcaaga
     1801 acggcctggc gctgcacctc atggcccagg gactgtacaa ccactctacc accacggtcc
     1861 aggacaaaga cgagagcgag tagctgtggc cagctccggg gcccgcggtc tatcggcgct
     1921 catgccacct ccaggctcca ccgggctgcc gcttcaccgg gcccactccg ccatgcagag
     1981 acgatcgcta aggagggagg catcaatcag ggcgacagag aaagcgagca agaaaaagca
     2041 atcctccgag tggacattca cattggaaca aaacggtttt tgaaacggga gaaagactcg
     2101 gacaggtgct atgggggaaa aataaagatc tattctctaa ctcactgtat aaatgaaact
     2161 gcgaattcct taaagctcta tctagccaaa ctgcttacga ccgtgtatat atttaatttc
     2221 aggtaaggaa aacaaatatg tgtagcgatc tctatttgct ggacattttt attaatctca
     2281 tttattattg ttataattat tataattatt ataattattt tatccctcct ccctaccttg
     2341 ctgctccccc ccagcccagt ttcgttttcg ttgctctttt cctttgaatg tttttgcttc
     2401 tctgggtacc tcctgcaccc ccaatgctgg ccctggtttc tctgggactt ttctttgtgt
     2461 gagtgtgagt gtgtttcctt gcgtgtcgcc cctcctctac ccacccagaa ttcttctatt
     2521 ggtcttgtct atccttcccc taagtccctt tccttttccg gagactcctt gagaaataca
     2581 accccacaga ctgcgagact gaaccgccgc tacaagccaa agattttatt atgttcagaa
     2641 acctgtagtc tggaataaaa tgttcactgt gttcacga
//
