LOCUS       NM_001107888            1848 bp    mRNA    linear   ROD 20-MAR-2023
DEFINITION  Rattus norvegicus EMG1 N1-specific pseudouridine methyltransferase
            (Emg1), mRNA.
ACCESSION   NM_001107888 XM_001062108 XM_232345
VERSION     NM_001107888.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 1848)
  AUTHORS   Baltz AG, Munschauer M, Schwanhausser B, Vasile A, Murakawa Y,
            Schueler M, Youngs N, Penfold-Brown D, Drew K, Milek M, Wyler E,
            Bonneau R, Selbach M, Dieterich C and Landthaler M.
  TITLE     The mRNA-bound proteome and its global occupancy profile on
            protein-coding transcripts
  JOURNAL   Mol Cell 46 (5), 674-690 (2012)
   PUBMED   22681889
REFERENCE   2  (bases 1 to 1848)
  AUTHORS   Castello A, Fischer B, Eichelbaum K, Horos R, Beckmann BM, Strein
            C, Davey NE, Humphreys DT, Preiss T, Steinmetz LM, Krijgsveld J and
            Hentze MW.
  TITLE     Insights into RNA biology from an atlas of mammalian mRNA-binding
            proteins
  JOURNAL   Cell 149 (6), 1393-1406 (2012)
   PUBMED   22658674
REFERENCE   3  (bases 1 to 1848)
  AUTHORS   Wu X, Sandhu S, Patel N, Triggs-Raine B and Ding H.
  TITLE     EMG1 is essential for mouse pre-implantation embryo development
  JOURNAL   BMC Dev Biol 10, 99 (2010)
   PUBMED   20858271
  REMARK    Publication Status: Online-Only
REFERENCE   4  (bases 1 to 1848)
  AUTHORS   Wurm JP, Meyer B, Bahr U, Held M, Frolow O, Kotter P, Engels JW,
            Heckel A, Karas M, Entian KD and Wohnert J.
  TITLE     The ribosome assembly factor Nep1 responsible for Bowen-Conradi
            syndrome is a pseudouridine-N1-specific methyltransferase
  JOURNAL   Nucleic Acids Res 38 (7), 2387-2398 (2010)
   PUBMED   20047967
COMMENT     PROVISIONAL REFSEQ: This record has not yet been subject to final
            NCBI review. The reference sequence was derived from
            JACYVU010000150.1.
            
            [WARNING] On Mar 29, 2024 this sequence was replaced by
            NM_001107888.4.
            
            On Feb 10, 2021 this sequence version replaced NM_001107888.2.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            ##Evidence-Data-START##
            Transcript exon combination :: CO382828.1, SRR8487230.121685.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA5760389, SAMEA5760393
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-298               JACYVU010000150.1  316124-316421       c
            299-400             JACYVU010000150.1  310377-310478       c
            401-542             JACYVU010000150.1  310147-310288       c
            543-601             JACYVU010000150.1  309762-309820       c
            602-751             JACYVU010000150.1  309542-309691       c
            752-1848            JACYVU010000150.1  308139-309235       c
FEATURES             Location/Qualifiers
     source          1..1848
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="BN"
                     /db_xref="taxon:10116"
                     /chromosome="4"
                     /map="4q42"
     gene            1..1848
                     /gene="Emg1"
                     /gene_synonym="Grcc2f"
                     /note="EMG1 N1-specific pseudouridine methyltransferase"
                     /db_xref="GeneID:312706"
                     /db_xref="RGD:1307665"
     exon            1..298
                     /gene="Emg1"
                     /gene_synonym="Grcc2f"
                     /inference="alignment:Splign:2.1.0"
     CDS             131..865
                     /gene="Emg1"
                     /gene_synonym="Grcc2f"
                     /note="gene rich cluster, C2f; probable ribosome
                     biogenesis protein NEP1; EMG1 nucleolar protein homolog"
                     /codon_start=1
                     /product="ribosomal RNA small subunit methyltransferase
                     NEP1"
                     /protein_id="NP_001101358.2"
                     /db_xref="GeneID:312706"
                     /db_xref="RGD:1307665"
                     /translation="MAATSGGFQPRERRFSVQEQDWDPAPPKRPRLGAGSKCGGRRLI
                     VVLEGASLETVKVGKTYELLNCDKHKSMLLKNGRDPGEVRPDITHQSLLMLMDSPLNR
                     AGLLQVYIHTQKNVLIEVNPQTRIPRTFDRFCGLMVQLLHKLSVRAADGPQKLLKVIK
                     NPVSDHFPVGCMKIGTSFSVEDISDIRELVPSSDPVVFVVGAFAHGKVSVEYTEKMVS
                     ISNYPLSAALTCAKVTTAFEEVWGVI"
     exon            299..400
                     /gene="Emg1"
                     /gene_synonym="Grcc2f"
                     /inference="alignment:Splign:2.1.0"
     exon            401..542
                     /gene="Emg1"
                     /gene_synonym="Grcc2f"
                     /inference="alignment:Splign:2.1.0"
     exon            543..601
                     /gene="Emg1"
                     /gene_synonym="Grcc2f"
                     /inference="alignment:Splign:2.1.0"
     exon            602..751
                     /gene="Emg1"
                     /gene_synonym="Grcc2f"
                     /inference="alignment:Splign:2.1.0"
     exon            752..1848
                     /gene="Emg1"
                     /gene_synonym="Grcc2f"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 aggcttccgt acaggtatcg ccattttcaa gcgccctctt tcaaaatggc agctagaagg
       61 aagtttgcag tgaggcggac gtgaagttct aacttaagga ccttagcgca acttccggtc
      121 tgcttacaag atggctgcga ccagtggtgg cttccaacct cgtgagcggc gtttttcagt
      181 gcaggaacag gactgggacc ctgcgccgcc taagagaccc cggcttgggg caggaagcaa
      241 gtgtggaggc cgtaggctca ttgtggtgtt ggaaggggcc agtctggaga cagtcaaggt
      301 agggaaaact tacgagctac tcaactgtga caagcacaag tccatgttgt tgaagaatgg
      361 acgggaccca ggggaagtca gaccagacat cacccaccag agcttgctga tgcttatgga
      421 cagccccctg aaccgagctg gcttgctcca ggtttatatc cacacacaga agaatgtgct
      481 cattgaagtg aacccccaga ctcggattcc taggaccttt gaccgatttt gtggcctcat
      541 ggttcagctt ttacacaaac tgagcgtccg agcagctgac ggccctcaga agcttttgaa
      601 ggtaattaag aatccagtgt ctgaccactt cccagttggc tgtatgaaaa ttggcacttc
      661 cttttctgtt gaggacatca gtgacattcg agagttggtg cccagtagtg acccagttgt
      721 tttcgtagtg ggggcctttg cccatggcaa ggtcagtgtg gagtacacag aaaagatggt
      781 gtccatcagc aactacccac tctctgccgc gctgacctgt gctaaagtta ccacagcttt
      841 tgaagaagta tggggcgtca tttgagaaca gcagtgcctg gctctcagac aggaaggact
      901 cttggcgttg tctgttgcct cctaagatct gctggaagat aatcttctgt accaagaagg
      961 cagagtgggg agccaatgct gtatatttgt tttttgtttt tcctataaag agtttttttt
     1021 tttttttttt ttttcagacc tgattgtttt gaggtttctt aaactcagca gtacatggta
     1081 gtgtaaactg ttcgccttag attcaggcat tccagacttt gatttcctag atttctgtgg
     1141 ctctcaagga gaccctgatg acctcagctt tcgcaagcac agaagagtat gtatgaaaga
     1201 aagcagtcta ggatgctagc agtgaaaaac tgaacaaaca gaagtgattt tatctggtat
     1261 agttccaaga tagaaaaagt ggagcaggca gggccttgta ccctcccctc cccccatggg
     1321 ggggtggcgg tatcggcaca tatacaatcg tagtagattg gcagaagaga aacaacagat
     1381 tcctggctag aggggagaga taaggcaacg tgcatggggg agtccaaagg gggtgcagcc
     1441 cctttgctcc tcccacaaga gcttcccctt tggctgtcgc agaggcacag ccgggagtac
     1501 tgtgacttcc agctcctggt gtctctgctc cggggtgagg agtgctgtga gaagggagac
     1561 aagtagtttc tgcaccagtc cagctctcgg ccacctacaa ggcaaggggt gtggtcagga
     1621 gggtgtcctt actaacaagg aaagtagaac ccacgggctg gagagctggc tcagcggtta
     1681 agagcactga ctgctcttct aaaggtcccg agttcaattg ccagcaacta catggtggct
     1741 cacaaccatc tgtaatgaga tctgatgccc tcttctgtgt agctgaagaa agtgacagtg
     1801 tactcataca cataaaataa ataaatctta aaaaacaaca acaaaaaa
//
