LOCUS       NM_053719               1458 bp    mRNA    linear   ROD 03-APR-2024
DEFINITION  Rattus norvegicus embigin (Emb), mRNA.
ACCESSION   NM_053719
VERSION     NM_053719.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 1458)
  AUTHORS   Forero-Quintero,L.S., Ames,S., Schneider,H.P., Thyssen,A.,
            Boone,C.D., Andring,J.T., McKenna,R., Casey,J.R., Deitmer,J.W. and
            Becker,H.M.
  TITLE     Membrane-anchored carbonic anhydrase IV interacts with
            monocarboxylate transporters via their chaperones CD147 and GP70
  JOURNAL   J Biol Chem 294 (2), 593-607 (2019)
   PUBMED   30446621
  REMARK    GeneRIF: This study analyzed the direct interaction between CAIV
            and the two Monocarboxylate transporters (MCTs) chaperones basigin
            (CD147) and embigin (GP70).
REFERENCE   2  (bases 1 to 1458)
  AUTHORS   Ovens,M.J., Manoharan,C., Wilson,M.C., Murray,C.M. and
            Halestrap,A.P.
  TITLE     The inhibition of monocarboxylate transporter 2 (MCT2) by
            AR-C155858 is modulated by the associated ancillary protein
  JOURNAL   Biochem J 431 (2), 217-225 (2010)
   PUBMED   20695846
  REMARK    GeneRIF: Expression of active MCT2 at the plasma membrane of
            oocytes was significantly enhanced by co-expression of exogenous
            embigin.
REFERENCE   3  (bases 1 to 1458)
  AUTHORS   Wilson,M.C., Meredith,D., Bunnun,C., Sessions,R.B. and
            Halestrap,A.P.
  TITLE     Studies on the DIDS-binding site of monocarboxylate transporter 1
            suggest a homology model of the open conformation and a plausible
            translocation cycle
  JOURNAL   J Biol Chem 284 (30), 20011-20021 (2009)
   PUBMED   19473976
REFERENCE   4  (bases 1 to 1458)
  AUTHORS   Lain,E., Carnejac,S., Escher,P., Wilson,M.C., Lomo,T., Gajendran,N.
            and Brenner,H.R.
  TITLE     A novel role for embigin to promote sprouting of motor nerve
            terminals at the neuromuscular junction
  JOURNAL   J Biol Chem 284 (13), 8930-8939 (2009)
   PUBMED   19164284
  REMARK    GeneRIF: embigin enhances neural cell adhesion molecule-dependent
            neuromuscular adhesion and thereby modulates neuromuscular junction
            formation and plasticity.
REFERENCE   5  (bases 1 to 1458)
  AUTHORS   Wilson,M.C., Meredith,D., Fox,J.E., Manoharan,C., Davies,A.J. and
            Halestrap,A.P.
  TITLE     Basigin (CD147) is the target for organomercurial inhibition of
            monocarboxylate transporter isoforms 1 and 4: the ancillary protein
            for the insensitive MCT2 is EMBIGIN (gp70)
  JOURNAL   J Biol Chem 280 (29), 27213-27221 (2005)
   PUBMED   15917240
  REMARK    GeneRIF: MCT2 associates with gp70 and is insensitive to pCMBS;
            embigin (gp70) is an ancillary protein required to maintain the
            catalytic activity of MCT2
REFERENCE   6  (bases 1 to 1458)
  AUTHORS   Guenette,R.S., Sridhar,S., Herley,M., Mooibroek,M., Wong,P. and
            Tenniswood,M.
  TITLE     Embigin, a developmentally expressed member of the immunoglobulin
            super family, is also expressed during regression of prostate and
            mammary gland
  JOURNAL   Dev Genet 21 (4), 268-278 (1997)
   PUBMED   9438341
COMMENT     PROVISIONAL REFSEQ: This record has not yet been subject to final
            NCBI review. The reference sequence was derived from AJ009698.1.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AJ009698.1, BC061846.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMD00132261, SAMD00132262
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
FEATURES             Location/Qualifiers
     source          1..1458
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="Sprague-Dawley"
                     /db_xref="taxon:10116"
                     /chromosome="2"
                     /map="2q14"
     gene            1..1458
                     /gene="Emb"
                     /note="embigin"
                     /db_xref="GeneID:114511"
                     /db_xref="RGD:621067"
     exon            1..199
                     /gene="Emb"
                     /inference="alignment:Splign:2.1.0"
     CDS             91..1077
                     /gene="Emb"
                     /function="putative cell adhesion molecule"
                     /codon_start=1
                     /product="embigin precursor"
                     /protein_id="NP_446171.1"
                     /db_xref="GeneID:114511"
                     /db_xref="RGD:621067"
                     /translation="MRSHTGLRALVAPGCSLLLLYLLAATRPDRAVGDPADSAFTSLP
                     VREEMMAKYANLSLETYNISLTEQTRVSEQNITLERPSHLELECTFTATEDVMSMNVT
                     WKKDDALLETTDGFNTTKMGDTLYSQYRFTVFNSKQMGKYSCFLGEELRGTFNIRVPK
                     VHGKNKPLITYVGDSTVLKCECQNCLPLNWTWYMSNGTAQVPIDVHVNDKFDINGSYA
                     NETKLKVKHLLEEDGGSYWCRAAFPLGESEEHIKLVVLSFMVPLKPFLAIIAEVILLV
                     AIILLCEVYTQKKKNDPDDGKEFEQIEQLKSDDSNGIENNVPRYRKTDSGDQ"
     exon            200..289
                     /gene="Emb"
                     /inference="alignment:Splign:2.1.0"
     exon            290..479
                     /gene="Emb"
                     /inference="alignment:Splign:2.1.0"
     exon            480..562
                     /gene="Emb"
                     /inference="alignment:Splign:2.1.0"
     exon            563..690
                     /gene="Emb"
                     /inference="alignment:Splign:2.1.0"
     exon            691..970
                     /gene="Emb"
                     /inference="alignment:Splign:2.1.0"
     exon            971..1004
                     /gene="Emb"
                     /inference="alignment:Splign:2.1.0"
     exon            1005..1059
                     /gene="Emb"
                     /inference="alignment:Splign:2.1.0"
     exon            1060..1454
                     /gene="Emb"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 ttaccagcaa gtgtgcatag ggacaaacgg agaccgatcg cagcaggctg agggtgcaat
       61 aacgacctcc tggaccgacc cgcccgcgcc atgcgctcgc acactggctt gagggcgctt
      121 gtggcgcctg gttgctcgct cctccttcta tatcttctgg cggcgacgcg cccggatcgg
      181 gctgtgggcg atcccgcaga ctcagcgttt acaagtctac ctgttcgaga ggaaatgatg
      241 gctaagtacg caaatctgtc cttggagacc tataatatct cactgacaga acagaccaga
      301 gtatcagaac aaaacataac tttggaaaga ccttctcacc tggaactcga atgcacgttc
      361 acggcaactg aggatgtgat gtcaatgaat gtgacttgga agaaagatga cgcgctcctt
      421 gagactactg atggtttcaa tacaactaaa atgggggaca ccttatacag tcaatacagg
      481 ttcaccgttt ttaatagcaa acaaatggga aaatactctt gtttccttgg agaagagctc
      541 agaggaacct ttaatatcag agtacccaaa gttcatggga aaaacaagcc tttgatcact
      601 tacgtggggg attctactgt gctgaagtgt gaatgtcaaa attgtcttcc tttaaattgg
      661 acttggtaca tgagtaatgg aactgcacag gttcccatcg atgttcacgt gaatgataag
      721 tttgatataa atggttccta tgctaacgaa acaaagctca aggtaaagca ccttttggag
      781 gaagatggag gatcctactg gtgccgggca gccttcccgt taggggagag tgaggaacac
      841 attaagctgg ttgtgctgag tttcatggtg cctctcaagc cattccttgc cataattgct
      901 gaagtcatcc tcttagtggc catcatcctg ctttgtgaag tgtacacgca gaagaaaaag
      961 aatgacccag atgatgggaa agaatttgaa caaatcgaac agttgaaatc agatgatagc
     1021 aatggaatag aaaacaatgt cccccggtac agaaaaacgg actctgggga tcagtgaatc
     1081 caaaaagaac tttttgcctt gggaagacgt acagcactgg agtttgaatt tcagcattca
     1141 ttggagaaca tccgagtttt agatttttcc gaagacaaag atgccctcac tgtgcccagg
     1201 agtagtttcg taaacaatat agaccatctc ctacctaaag atgtattttc attgtgtaat
     1261 tgcttttcat taaagcaagg tacattgttt cagctgtgtt ctgtgagctg taacctagat
     1321 aataaagttt accctctctg ggagatcagg gggacagggc agacaccagc aaaatcagtt
     1381 aataacccac aaagtaaata ttgctaaaga tctgtttatt accaaagaat ttattaaaga
     1441 gaaagtgttt gccaaaaa
//
