LOCUS       NM_001110141            4118 bp    mRNA    linear   ROD 03-APR-2024
DEFINITION  Rattus norvegicus eukaryotic translation initiation factor 5B
            (Eif5b), mRNA.
ACCESSION   NM_001110141 XM_001055509 XM_001057591 XM_001057637 XM_218162
VERSION     NM_001110141.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 4118)
  AUTHORS   Hinnebusch,A.G.
  TITLE     The scanning mechanism of eukaryotic translation initiation
  JOURNAL   Annu Rev Biochem 83, 779-812 (2014)
   PUBMED   24499181
  REMARK    Review article
REFERENCE   2  (bases 1 to 4118)
  AUTHORS   Baltz,A.G., Munschauer,M., Schwanhausser,B., Vasile,A.,
            Murakawa,Y., Schueler,M., Youngs,N., Penfold-Brown,D., Drew,K.,
            Milek,M., Wyler,E., Bonneau,R., Selbach,M., Dieterich,C. and
            Landthaler,M.
  TITLE     The mRNA-bound proteome and its global occupancy profile on
            protein-coding transcripts
  JOURNAL   Mol Cell 46 (5), 674-690 (2012)
   PUBMED   22681889
REFERENCE   3  (bases 1 to 4118)
  AUTHORS   Castello,A., Fischer,B., Eichelbaum,K., Horos,R., Beckmann,B.M.,
            Strein,C., Davey,N.E., Humphreys,D.T., Preiss,T., Steinmetz,L.M.,
            Krijgsveld,J. and Hentze,M.W.
  TITLE     Insights into RNA biology from an atlas of mammalian mRNA-binding
            proteins
  JOURNAL   Cell 149 (6), 1393-1406 (2012)
   PUBMED   22658674
REFERENCE   4  (bases 1 to 4118)
  AUTHORS   Bohnsack,M.T., Regener,K., Schwappach,B., Saffrich,R.,
            Paraskeva,E., Hartmann,E. and Gorlich,D.
  TITLE     Exp5 exports eEF1A via tRNA from nuclei and synergizes with other
            transport pathways to confine translation to the cytoplasm
  JOURNAL   EMBO J 21 (22), 6205-6215 (2002)
   PUBMED   12426392
REFERENCE   5  (bases 1 to 4118)
  AUTHORS   Wilson,S.A., Sieiro-Vazquez,C., Edwards,N.J., Iourin,O.,
            Byles,E.D., Kotsopoulou,E., Adamson,C.S., Kingsman,S.M.,
            Kingsman,A.J. and Martin-Rendon,E.
  TITLE     Cloning and characterization of hIF2, a human homologue of
            bacterial translation initiation factor 2, and its interaction with
            HIV-1 matrix
  JOURNAL   Biochem J 342 (Pt 1) (Pt 1), 97-103 (1999)
   PUBMED   10432305
REFERENCE   6  (bases 1 to 4118)
  AUTHORS   Ohsawa,K., Imai,Y., Ito,D. and Kohsaka,S.
  TITLE     Molecular cloning and characterization of annexin V-binding
            proteins with highly hydrophilic peptide structure
  JOURNAL   J Neurochem 67 (1), 89-97 (1996)
   PUBMED   8667030
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            JAXUCZ010000009.1.
            
            On or before Oct 26, 2007 this sequence version replaced
            XM_001057637.1, XM_001057591.1, XM_218162.4, XM_001055509.1.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC166424.1, SRR26643295.35005.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA5760383, SAMEA5760386
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-183               JAXUCZ010000009.1  47718706-47718888
            184-309             JAXUCZ010000009.1  47741267-47741392
            310-394             JAXUCZ010000009.1  47741483-47741567
            395-1064            JAXUCZ010000009.1  47742303-47742972
            1065-1282           JAXUCZ010000009.1  47745005-47745222
            1283-1433           JAXUCZ010000009.1  47745591-47745741
            1434-1532           JAXUCZ010000009.1  47756053-47756151
            1533-1622           JAXUCZ010000009.1  47757001-47757090
            1623-1697           JAXUCZ010000009.1  47757930-47758004
            1698-1978           JAXUCZ010000009.1  47758317-47758597
            1979-2086           JAXUCZ010000009.1  47759597-47759704
            2087-2197           JAXUCZ010000009.1  47759919-47760029
            2198-2265           JAXUCZ010000009.1  47760730-47760797
            2266-2407           JAXUCZ010000009.1  47761777-47761918
            2408-2539           JAXUCZ010000009.1  47766016-47766147
            2540-2722           JAXUCZ010000009.1  47766637-47766819
            2723-2884           JAXUCZ010000009.1  47766929-47767090
            2885-3016           JAXUCZ010000009.1  47767780-47767911
            3017-3148           JAXUCZ010000009.1  47770036-47770167
            3149-3225           JAXUCZ010000009.1  47770255-47770331
            3226-3390           JAXUCZ010000009.1  47770420-47770584
            3391-3529           JAXUCZ010000009.1  47771569-47771707
            3530-3691           JAXUCZ010000009.1  47772441-47772602
            3692-4118           JAXUCZ010000009.1  47772863-47773289
FEATURES             Location/Qualifiers
     source          1..4118
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="BN"
                     /db_xref="taxon:10116"
                     /chromosome="9"
                     /map="9q21"
     gene            1..4118
                     /gene="Eif5b"
                     /note="eukaryotic translation initiation factor 5B"
                     /db_xref="GeneID:308306"
                     /db_xref="RGD:735017"
     exon            1..183
                     /gene="Eif5b"
                     /inference="alignment:Splign:2.1.0"
     CDS             149..3799
                     /gene="Eif5b"
                     /EC_number="3.6.5.3"
                     /note="eIF-5B; translation initiation factor IF-2; annexin
                     V-binding protein ABP-7"
                     /codon_start=1
                     /product="eukaryotic translation initiation factor 5B"
                     /protein_id="NP_001103611.1"
                     /db_xref="GeneID:308306"
                     /db_xref="RGD:735017"
                     /translation="MGKKQKNKSEDSTKDDTDLGALAAEIEGAGAAKEQEPQKGKGKK
                     KKEKKKQDFDENDILRELEELSLEAQGIGADRDAATVKPTENNEEESASKQDKKKKGQ
                     KGKKTSFDENDSEELEDKDSKSKKPARPNSEVLLSGSEDADDPNKLSKKGKKAQKSTK
                     KRDGSEEDEDNSKRSKERSRVNSSGESGGESDEFLQSRKGQKKNQKNKSVPTIDSGNE
                     DDDSSFKIKTVAQKKAEKKERERKKREEEKAKLRKVKEKEELEKGRKEQSKQREPQKR
                     PDEEVLVLRGTPDAGAASEEKGDIAATLEDDNEGDKKKKDKKKKKTEKDDKEKEKKKG
                     PSKSTVKAIQEALAKLREEEERQKREEEERIKRLEELEAKRKEEERLEQEKRERKKQK
                     EKERKERLKKEGKLLTKSQREARARAEVTLRHLQAQGVEVPSKDSLPKKRPVYEDKKK
                     KKTPQQLESKEALETVEVSAPVEVVDQGVPEKEETPPSVDAEEDEETEDAGLDDWEAM
                     ASDEEREKEGNMIHIEVEENPEEEEEEEEDEDEEDSEDEEDEGDSEGSDGDEEDYKLS
                     DEKDLGKAGDTKPNKDASSDSEYDSDDDRTKEERAYDKAKRRIEKRRLEHGKNVNTEK
                     LRAPIICVLGHVDTGKTKILDKLRHTHVQDGEAGGITQQIGATNVPLEAINEQTKMIK
                     NFDRENVRIPGMLIIDTPGHESFSNLRNRGSSLCDIAILVVDIMHGLEPQTIESINIL
                     KSKKCPFIVALNKIDRLYDWKKSPDSDVAVTLKKQKKNTKDEFEERAKAIIVEFAQQG
                     LNAALFYENKDPRTFVSLVPTSAHTGDGMGSLIYLLVELTQTMLSKRLAHCEELRAQV
                     MEVKALPGMGTTIDVILINGRLKEGDTIIVPGVEGPIVTQIRGLLLPPPMKELRVKNQ
                     YEKHKEVEAAQGVKILGKDLEKTLAGLPLLVAYKDDEIPVLKDELIHELKQTLNAIKL
                     EEKGVYVQASTLGSLEALLEFLKTSEVPYAGINIGPVHKKDVMKASVMLEHDPQYAVI
                     LAFDVRIERDAQEMADSLGVRIFSAEIIYHLFDAFTKYRQDYKKQKQEEFKHIAVFPC
                     KMKILPQYIFNSRDPIVIGVTVEAGQVKQGTPMCVPSKNFVDIGIVTSIEINHKQVDV
                     AKKGQEVCVKIEPIPGESPKMFGRHFEATDILVSKISRQSIDALKDWFRDEMQKSDWQ
                     LIVELKKVFEII"
     exon            184..309
                     /gene="Eif5b"
                     /inference="alignment:Splign:2.1.0"
     exon            310..394
                     /gene="Eif5b"
                     /inference="alignment:Splign:2.1.0"
     exon            395..1064
                     /gene="Eif5b"
                     /inference="alignment:Splign:2.1.0"
     exon            1065..1282
                     /gene="Eif5b"
                     /inference="alignment:Splign:2.1.0"
     exon            1283..1433
                     /gene="Eif5b"
                     /inference="alignment:Splign:2.1.0"
     exon            1434..1532
                     /gene="Eif5b"
                     /inference="alignment:Splign:2.1.0"
     exon            1533..1622
                     /gene="Eif5b"
                     /inference="alignment:Splign:2.1.0"
     exon            1623..1697
                     /gene="Eif5b"
                     /inference="alignment:Splign:2.1.0"
     exon            1698..1978
                     /gene="Eif5b"
                     /inference="alignment:Splign:2.1.0"
     exon            1979..2086
                     /gene="Eif5b"
                     /inference="alignment:Splign:2.1.0"
     exon            2087..2197
                     /gene="Eif5b"
                     /inference="alignment:Splign:2.1.0"
     exon            2198..2265
                     /gene="Eif5b"
                     /inference="alignment:Splign:2.1.0"
     exon            2266..2407
                     /gene="Eif5b"
                     /inference="alignment:Splign:2.1.0"
     exon            2408..2539
                     /gene="Eif5b"
                     /inference="alignment:Splign:2.1.0"
     exon            2540..2722
                     /gene="Eif5b"
                     /inference="alignment:Splign:2.1.0"
     exon            2723..2884
                     /gene="Eif5b"
                     /inference="alignment:Splign:2.1.0"
     exon            2885..3016
                     /gene="Eif5b"
                     /inference="alignment:Splign:2.1.0"
     exon            3017..3148
                     /gene="Eif5b"
                     /inference="alignment:Splign:2.1.0"
     exon            3149..3225
                     /gene="Eif5b"
                     /inference="alignment:Splign:2.1.0"
     exon            3226..3390
                     /gene="Eif5b"
                     /inference="alignment:Splign:2.1.0"
     exon            3391..3529
                     /gene="Eif5b"
                     /inference="alignment:Splign:2.1.0"
     exon            3530..3691
                     /gene="Eif5b"
                     /inference="alignment:Splign:2.1.0"
     exon            3692..4118
                     /gene="Eif5b"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 cccagtgcgc gggtctgtgg agagccgggt gcgagaggcg gcggcgcgag ggggaccgag
       61 ccgagcgggc attggagtcg gctgggagac agtgggtctg tgagacaccg aaccgagggg
      121 ccgtggccac gagcgccgct gacaagcgat ggggaagaaa cagaaaaaca agagcgaaga
      181 cagtaccaag gatgatactg acttgggcgc cttggctgcg gaaatagaag gagctggtgc
      241 tgccaaggag caggagcctc agaagggaaa ggggaagaag aaaaaggaga aaaagaagca
      301 agactttgat gagaacgaca ttctgagaga gctggaggag ttgtctctgg aagcccaagg
      361 catcggagct gacagagatg ccgctacagt caagcctaca gaaaacaatg aagaagaatc
      421 tgcctcaaaa caagataaaa agaagaaagg acaaaaaggc aagaaaacaa gttttgatga
      481 gaatgatagt gaagaattgg aagataaaga ttcaaaatca aaaaagcctg ccagacctaa
      541 ctcagaggtg ctcctctctg ggagtgagga tgcagatgat cctaataaac tttctaaaaa
      601 aggtaaaaaa gcccagaaat caactaaaaa acgggatggg tcggaagagg atgaagacaa
      661 tagcaaaaga agtaaagaac gttcgagagt aaactcctca ggtgaaagtg gtggtgagtc
      721 tgatgaattt ttgcagtcca gaaaaggaca gaaaaaaaat cagaagaaca agtcagttcc
      781 taccatagac agtgggaatg aggacgatga ctcttccttc aaaattaaga cggtggccca
      841 gaagaaggca gaaaagaaag agcgtgagag gaaaaagaga gaagaggaga aagcaaagtt
      901 gcgaaaggtg aaagagaaag aagagctaga gaagggcagg aaggagcaga gtaagcagag
      961 ggagcctcag aagagaccgg acgaggaggt gttggtgctc agagggactc ctgatgcagg
     1021 tgctgcctca gaagagaaag gagacattgc tgcaactctg gaagatgaca atgaaggaga
     1081 caaaaagaaa aaagataaga agaaaaagaa aacagaaaaa gatgataaag aaaaagagaa
     1141 gaagaaaggc ccaagcaaat ccactgttaa agctatccag gaagctttgg ctaagctcag
     1201 agaggaggag gagagacaga agagagagga ggaggaaagg attaagcggc ttgaagaact
     1261 agaagccaaa cgaaaagaag aggaacgatt agaacaagaa aaaagagaaa ggaaaaagca
     1321 aaaagaaaaa gaaagaaaag aacggttaaa aaaagaagga aaactgttaa ctaagtccca
     1381 gagagaagcc agagccagag ctgaagtgac cctgagacac ctccaagcgc agggtgttga
     1441 agtgccatca aaagactcgt tgccaaagaa gaggccagtg tatgaagata agaagaagaa
     1501 gaaaacacca cagcagctgg agagtaaaga agcacttgag actgtggaag tcagtgcccc
     1561 tgtggaagtt gtggaccaag gagtaccaga aaaggaggag acaccgcctt ctgttgacgc
     1621 agaggaagac gaagaaactg aagatgctgg gctcgatgac tgggaagcta tggccagtga
     1681 tgaggagaga gaaaaagaag gaaatatgat tcacatagaa gtagaagaaa accctgagga
     1741 agaggaggag gaagaagagg atgaagatga agaagacagt gaagatgagg aagacgaagg
     1801 ggacagtgag ggcagcgatg gggacgagga ggactacaag ctatcggacg agaaggactt
     1861 ggggaaagct ggagacacga agcccaacaa agacgctagc tcagactccg agtatgactc
     1921 tgatgacgat cggactaaag aagagcgggc gtacgacaaa gcaaaacgga gaattgagaa
     1981 acgacgactt gaacatggta aaaatgtaaa tacagaaaag ctaagagccc ctatcatctg
     2041 tgtgcttgga catgtagaca cagggaagac aaaaattcta gataagctcc gccacactca
     2101 tgtacaagat ggtgaagcag gtggcatcac acagcagatt ggtgccacaa atgttcctct
     2161 cgaagctatt aatgaacaaa ctaaaatgat taaaaatttt gacagagaga atgtccgcat
     2221 tccagggatg ctgattattg acactcctgg acatgagtct ttcagtaacc tgagaaacag
     2281 aggaagctct ctctgtgata ttgccatttt agttgttgac attatgcatg gtttagagcc
     2341 ccagacaatt gaatcaatca acattctcaa atccaaaaaa tgtcccttta ttgttgcact
     2401 taataagatt gacaggttat atgattggaa gaagagtcct gattcggatg tggctgttac
     2461 tttaaaaaag cagaagaaga atacaaagga tgagtttgag gagcgtgcaa aggccattat
     2521 tgtagaattt gcacaacagg gtttaaatgc tgctttgttt tatgagaata aagatccccg
     2581 cacttttgtg tccctggtac cgacctctgc acacactggt gatggcatgg gaagcctcat
     2641 ctaccttctt gtggaattaa ctcaaactat gttgagcaag aggcttgctc actgtgagga
     2701 gctgagagca caagtcatgg aggttaaagc tctcccagga atgggcacca caatagatgt
     2761 catattgatt aatggacgtt tgaaggaagg ggacacaatc attgttcctg gagtagaagg
     2821 gcccattgtc actcagattc gaggcctcct tttacctcct cctatgaagg aattacgcgt
     2881 gaagaaccaa tatgaaaagc ataaagaagt ggaagcagcc cagggagtga agattcttgg
     2941 aaaagacctt gagaaaacgc tggctggttt acctctccta gtggcctata aggacgacga
     3001 aatcccagtt ctcaaagatg aattgatcca tgagttaaag cagacgctga atgctatcaa
     3061 attggaagaa aaaggagtct atgttcaggc atctacactg ggctctttgg aagctcttct
     3121 tgaatttctg aaaacatcag aggtgccata tgcgggaatt aacattggcc cagtccacaa
     3181 gaaggatgtt atgaaggctt cggtgatgct ggaacatgat ccgcagtatg cagtaatttt
     3241 ggcctttgat gtaagaatcg aacgggatgc gcaagagatg gctgatagtt tgggagttag
     3301 aatctttagt gcagaaatta tttatcattt atttgatgcc tttacaaaat acagacaaga
     3361 ctacaagaaa caaaaacaag aagaatttaa gcacatagca gtatttccct gcaagatgaa
     3421 gattctccct cagtacattt tcaattcccg agatccaata gtaatagggg tcactgtgga
     3481 ggctggtcaa gtgaaacaag gaacacctat gtgtgttcca agcaaaaatt ttgttgacat
     3541 tgggatagta acaagcattg aaataaacca caagcaggtg gatgttgcaa aaaaaggaca
     3601 agaggtctgt gtcaaaatag aacctatccc tggggaatct ccaaaaatgt ttgggagaca
     3661 cttcgaagct acagacattc ttgtcagcaa gatcagccgg cagtccatcg atgcactcaa
     3721 agactggttc agagacgaaa tgcagaagag tgactggcag cttattgtgg agctgaagaa
     3781 agtatttgaa atcatctaac tttcccacat gggacagaat ttggagtaaa tgtaatatta
     3841 tgttgtgata ttaccaacag aacagacatt tggacgctga tggacttagg tgtataaaat
     3901 gttttccatg agaaaccaag caacttacac tggtttgaca gtggtcagtt ccccgcccca
     3961 cagtccagcg tgcctgcact cacctgccct tcctctaaga cttggctgct gttttaaagt
     4021 ttgcccttcc tcaaatttgg atttttatta caaatctaaa tctttcaatt ttatactgat
     4081 taaatcagtc ctgcagtatt tgattaacta agcttcta
//
