LOCUS       NM_001017374            5008 bp    mRNA    linear   ROD 26-FEB-2024
DEFINITION  Rattus norvegicus eukaryotic translation initiation factor 4, gamma
            2 (Eif4g2), mRNA.
ACCESSION   NM_001017374 XM_341907
VERSION     NM_001017374.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 5008)
  AUTHORS   Guo Z, Neilson LJ, Zhong H, Murray PS, Zanivan S and Zaidel-Bar R.
  TITLE     E-cadherin interactome complexity and robustness resolved by
            quantitative proteomics
  JOURNAL   Sci Signal 7 (354), rs7 (2014)
   PUBMED   25468996
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5008)
  AUTHORS   Distler U, Schmeisser MJ, Pelosi A, Reim D, Kuharev J, Weiczner R,
            Baumgart J, Boeckers TM, Nitsch R, Vogt J and Tenzer S.
  TITLE     In-depth protein profiling of the postsynaptic density from mouse
            hippocampus using data-independent acquisition proteomics
  JOURNAL   Proteomics 14 (21-22), 2607-2613 (2014)
   PUBMED   25211037
REFERENCE   3  (bases 1 to 5008)
  AUTHORS   Hinnebusch AG.
  TITLE     The scanning mechanism of eukaryotic translation initiation
  JOURNAL   Annu Rev Biochem 83, 779-812 (2014)
   PUBMED   24499181
  REMARK    Review article
REFERENCE   4  (bases 1 to 5008)
  AUTHORS   Kar AN, MacGibeny MA, Gervasi NM, Gioio AE and Kaplan BB.
  TITLE     Intra-axonal synthesis of eukaryotic translation initiation factors
            regulates local protein synthesis and axon growth in rat
            sympathetic neurons
  JOURNAL   J Neurosci 33 (17), 7165-7174 (2013)
   PUBMED   23616526
  REMARK    GeneRIF: Local protein synthesis was found to be regulated by
            Eif4g2 (and Eif2b2) in the axon.
REFERENCE   5  (bases 1 to 5008)
  AUTHORS   Hinnebusch AG and Lorsch JR.
  TITLE     The mechanism of eukaryotic translation initiation: new insights
            and challenges
  JOURNAL   Cold Spring Harb Perspect Biol 4 (10), a011544 (2012)
   PUBMED   22815232
  REMARK    Review article
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 5008)
  AUTHORS   Kimball SR, Horetsky RL, Ron D, Jefferson LS and Harding HP.
  TITLE     Mammalian stress granules represent sites of accumulation of
            stalled translation initiation complexes
  JOURNAL   Am J Physiol Cell Physiol 284 (2), C273-C284 (2003)
   PUBMED   12388085
REFERENCE   7  (bases 1 to 5008)
  AUTHORS   Henis-Korenblit S, Shani G, Sines T, Marash L, Shohat G and Kimchi
            A.
  TITLE     The caspase-cleaved DAP5 protein supports internal ribosome entry
            site-mediated translation of death proteins
  JOURNAL   Proc Natl Acad Sci U S A 99 (8), 5400-5405 (2002)
   PUBMED   11943866
REFERENCE   8  (bases 1 to 5008)
  AUTHORS   Pak BJ and Pang SC.
  TITLE     Developmental regulation of the translational repressor NAT1 during
            cardiac development
  JOURNAL   J Mol Cell Cardiol 31 (9), 1717-1724 (1999)
   PUBMED   10471355
REFERENCE   9  (bases 1 to 5008)
  AUTHORS   Qian X, Balestra ME, Yamanaka S, Boren J, Lee I and Innerarity TL.
  TITLE     Low expression of the apolipoprotein B mRNA-editing transgene in
            mice reduces LDL levels but does not cause liver dysplasia or
            tumors
  JOURNAL   Arterioscler Thromb Vasc Biol 18 (6), 1013-1020 (1998)
   PUBMED   9633945
REFERENCE   10 (bases 1 to 5008)
  AUTHORS   Yamanaka S, Poksay KS, Arnold KS and Innerarity TL.
  TITLE     A novel translational repressor mRNA is edited extensively in
            livers containing tumors caused by the transgene expression of the
            apoB mRNA-editing enzyme
  JOURNAL   Genes Dev 11 (3), 321-333 (1997)
   PUBMED   9030685
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from CB730161.1, BC099117.1 and
            AB256044.1.
            
            On or before Jul 25, 2007 this sequence version replaced
            XM_341907.3, NM_001017374.1.
            
            Summary: Translation initiation is mediated by specific recognition
            of the cap structure by eukaryotic translation initiation factor 4F
            (eIF4F), which is a cap binding protein complex that consists of
            three subunits: eIF4A, eIF4E and eIF4G. The protein encoded by this
            gene shares similarity with the C-terminal region of eIF4G that
            contains the binding sites for eIF4A and eIF3; eIF4G, in addition,
            contains a binding site for eIF4E at the N-terminus. Unlike eIF4G,
            which supports cap-dependent and independent translation, this gene
            product functions as a general repressor of translation by forming
            translationally inactive complexes. In vitro and in vivo studies in
            human indicate that translation of this mRNA initiates exclusively
            at a non-AUG (GUG) codon. This also appears to be true for mouse
            and rat. [provided by RefSeq, Jul 2008].
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC099117.1, SRR8487226.30836.1
                                           [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMD00132261,
                                           SAMD00132262 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            non-AUG initiation codon :: PMID: 9049310, 15718103
            RefSeq Select criteria   :: based on single protein-coding
                                        transcript
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-139               CB730161.1         4-142
            140-3781            BC099117.1         27-3668
            3782-5008           AB256044.1         2009-3235
FEATURES             Location/Qualifiers
     source          1..5008
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="W"
                     /db_xref="taxon:10116"
                     /chromosome="1"
                     /map="1q33"
     gene            1..5008
                     /gene="Eif4g2"
                     /note="eukaryotic translation initiation factor 4, gamma
                     2"
                     /db_xref="GeneID:361628"
                     /db_xref="RGD:1305294"
     exon            1..215
                     /gene="Eif4g2"
                     /inference="alignment:Splign:2.1.0"
     exon            216..342
                     /gene="Eif4g2"
                     /inference="alignment:Splign:2.1.0"
     CDS             302..3022
                     /gene="Eif4g2"
                     /exception="alternative start codon"
                     /note="non-AUG (GUG) translation initiation codon"
                     /codon_start=1
                     /transl_except=(pos:302..304,aa:Met)
                     /product="eukaryotic translation initiation factor 4 gamma
                     2"
                     /protein_id="NP_001017374.2"
                     /db_xref="GeneID:361628"
                     /db_xref="RGD:1305294"
                     /translation="MESAIAEGGASRFSASSGGGGSRGAPQHYPKTAGNSEFLGKTPG
                     QNAQKWIPARSTRRDDNSAANNSANEKERHDAIFRKVRGILNKLTPEKFDKLCLELLN
                     VGVESKLILKGVILLIVDKALEEPKYSSLYAQLCLRLAEDAPNFDGPAAEGQPGQKQS
                     TTFRRLLISKLQDEFENRTRNVDVYDKRENPLLPEEEEQRAIAKIKMLGNIKFIGELG
                     KLDLIHESILHKCIKTLLEKKKRVQLKDMGEDLECLCQIMRTVGPRLDHERAKSLMDQ
                     YFARMCSLMLSKELPARIRFLLQDTVELREHHWVPRKAFLDNGPKTINQIRQDAVKDL
                     GVFIPAPMAQGRNDFFLEGPFMPPRMKMDRDPLGGLADMFGQMPGSGIGTGPGVIQDR
                     FSPTMGRHRSNQLFNGHGGHIMPPTQSQFGEMGGKFMKSQGLSQLYHNQSQGLLSQLQ
                     GQSKDMPPRFSKKGQLNADEISLRPAQSFLMNKNQVPKLQPQITMIPPSAQPPRTQTP
                     PLGQTPQLGLKTNPPLIQEKPAKTSKKPPPSKEELLKLTEAVVTEYLNSGNANDAVSG
                     VREMRAPKHFLPEMLSKVIILSLDRSDEDKEKASSLISLLKQEGIATSDNFMQAFLNV
                     LEQCPKLEVDIPLVKSYLAQFAARAIISELVSISELAQPLESGTHFPLFLLCLQQLAK
                     LQDREWLTELFQQSKVNMQKMLPEIDQNKDRMLEILEGKGLSFLFPLLKLEKELLKQI
                     KLDPSPQTIYKWIKDNISPKLHVDKGFVNILMTSFLQYISSEVSPPSDETDSSSAPSK
                     EQLEQEKQLLLSFKPVMQKFLHDHVDLQVSALYALQVHCYNSSFPKGMLLRFFVHFYD
                     MEIIEEEAFLAWKEDITQEFPGKGKALFQVNQWLTWLETAEEEESEEEAD"
     exon            343..408
                     /gene="Eif4g2"
                     /inference="alignment:Splign:2.1.0"
     exon            409..549
                     /gene="Eif4g2"
                     /inference="alignment:Splign:2.1.0"
     exon            550..652
                     /gene="Eif4g2"
                     /inference="alignment:Splign:2.1.0"
     exon            653..784
                     /gene="Eif4g2"
                     /inference="alignment:Splign:2.1.0"
     exon            785..851
                     /gene="Eif4g2"
                     /inference="alignment:Splign:2.1.0"
     exon            852..1003
                     /gene="Eif4g2"
                     /inference="alignment:Splign:2.1.0"
     exon            1004..1114
                     /gene="Eif4g2"
                     /inference="alignment:Splign:2.1.0"
     exon            1115..1198
                     /gene="Eif4g2"
                     /inference="alignment:Splign:2.1.0"
     exon            1199..1297
                     /gene="Eif4g2"
                     /inference="alignment:Splign:2.1.0"
     exon            1298..1436
                     /gene="Eif4g2"
                     /inference="alignment:Splign:2.1.0"
     exon            1437..1597
                     /gene="Eif4g2"
                     /inference="alignment:Splign:2.1.0"
     exon            1598..1711
                     /gene="Eif4g2"
                     /inference="alignment:Splign:2.1.0"
     exon            1712..1837
                     /gene="Eif4g2"
                     /inference="alignment:Splign:2.1.0"
     exon            1838..1942
                     /gene="Eif4g2"
                     /inference="alignment:Splign:2.1.0"
     exon            1943..2158
                     /gene="Eif4g2"
                     /inference="alignment:Splign:2.1.0"
     exon            2159..2417
                     /gene="Eif4g2"
                     /inference="alignment:Splign:2.1.0"
     exon            2418..2622
                     /gene="Eif4g2"
                     /inference="alignment:Splign:2.1.0"
     exon            2623..2834
                     /gene="Eif4g2"
                     /inference="alignment:Splign:2.1.0"
     exon            2835..2956
                     /gene="Eif4g2"
                     /inference="alignment:Splign:2.1.0"
     exon            2957..4989
                     /gene="Eif4g2"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3761..3766
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Eif4g2"
     polyA_site      3781
                     /gene="Eif4g2"
     regulatory      4970..4975
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Eif4g2"
     polyA_site      4989
                     /gene="Eif4g2"
ORIGIN      
        1 cagcgagccg gagctctatg gaggtggcgg cgggtaccag gtggtggctg cagcagctac
       61 tcctctgagc tgagtttcaa gccgtccccg tctccttcct cccccttccc tccccctttt
      121 ttgttttccg ttccccttcc ccctcccttc cccgtccccg acgaccggat ccagaggagg
      181 cagctgcggt agcagctgct gagttctcgg tgaaggcttt tcatttctac catcccctcc
      241 cctccccacc ccatccatta atattattct tttgaagatt cttcgttgtc aagccgccaa
      301 agtggagagt gcgattgcag aagggggtgc ttctcgtttc agtgcttctt cgggcggagg
      361 aggaagtagg ggtgcacctc agcactatcc caagactgct ggcaacagcg agttcctggg
      421 gaaaacccca gggcaaaacg ctcagaaatg gattcctgca cgaagcacta gacgagatga
      481 caactccgca gcaaacaact ccgcaaatga aaaagaacga catgatgcaa tcttcaggaa
      541 agtaagaggc atactaaata agcttactcc tgaaaagttt gacaagctat gccttgagct
      601 cctcaatgtg ggtgtagagt ctaaactcat ccttaaaggg gtcatactgc tgattgtgga
      661 caaagcccta gaagagccaa agtatagctc actgtatgct cagctatgtc tgcgattggc
      721 agaagatgca ccaaactttg atggcccagc agcagagggt caaccaggac agaagcaaag
      781 cacaacattc agacgcctct tgatttccaa attgcaagat gaatttgaaa accgaaccag
      841 aaatgttgat gtctatgata agcgtgaaaa tcccctcctc cctgaggagg aggaacagag
      901 agccattgct aagatcaaga tgttggggaa catcaaattc attggagaac ttggcaagct
      961 tgatcttatt catgaatcta tccttcataa gtgcatcaaa acacttttgg aaaagaagaa
     1021 gagagtccaa ctcaaagata tgggagagga tttggagtgc ctctgtcaga taatgaggac
     1081 agtgggacct cgattagacc atgaacgagc caagtcctta atggatcagt actttgccag
     1141 aatgtgttcc ttaatgttaa gtaaggaatt gccagccagg attcgtttcc tactgcagga
     1201 taccgtagag ttgcgagagc accattgggt tcctcgcaag gcttttcttg acaatggacc
     1261 aaagacgatc aatcaaatcc gtcaagatgc agtaaaagat ctaggagtgt ttattcctgc
     1321 tcctatggct caagggagga atgacttctt cctggaggga ccgttcatgc cgccaaggat
     1381 gaaaatggat agggacccac ttgggggact cgctgatatg tttggacaaa tgccaggtag
     1441 tggaattggt actggtccag gagttatcca ggatagattt tcacccacaa tgggacgtca
     1501 tcgttcaaat cagctcttca atggccatgg gggacacatc atgcctccca cgcaatcgca
     1561 gtttggagag atggggggca agtttatgaa aagccagggg ctaagccagc tctaccataa
     1621 ccagagtcag ggactcttat cccagctgca aggacagtcg aaggatatgc cacctcggtt
     1681 ttctaagaaa ggacagctta atgcagatga gattagtttg aggcctgctc agtcgtttct
     1741 aatgaataaa aatcaggtgc caaagcttca gccccagata actatgattc ctcccagtgc
     1801 acagccacca cgcactcaaa caccacctct gggacagaca cctcaacttg gtctcaaaac
     1861 taatccacca cttattcagg aaaagcctgc caagactagc aaaaagccac caccatcaaa
     1921 ggaagaacta cttaaactga ccgaagctgt tgtaactgag tatctgaaca gtggaaatgc
     1981 caacgacgct gtcagtggtg tgagagaaat gagagctcca aaacactttc ttcctgagat
     2041 gttaagcaaa gtgatcatcc tgtcacttga tagaagtgat gaagataaag aaaaagcaag
     2101 ctctttgatc agtttactca aacaggaagg gatagccaca agtgacaact ttatgcaggc
     2161 tttcctgaat gtattggagc agtgccccaa actggaggtt gacatcccct tggtgaaatc
     2221 ttacttggca cagtttgcgg ctcgtgctat aatttcagag ttggtgagca tttcagaact
     2281 agctcaacca ctggagagtg gcacccactt ccctctcttc ttactttgtc ttcaacaatt
     2341 agctaaatta caagaccgag aatggttaac cgaacttttt caacaaagca aggtcaatat
     2401 gcagaaaatg ctgccagaaa ttgatcagaa taaggatcga atgttggaga ttttggaagg
     2461 aaagggactg agtttcttat tcccactcct taaattggag aaggaactat tgaagcaaat
     2521 taagctggat ccatcccctc aaactatata taaatggatt aaagataaca tctctcccaa
     2581 acttcatgta gataaaggat ttgtgaacat cttaatgacc agcttcttac agtacatttc
     2641 tagtgaagtt agcccaccca gcgatgaaac agattcttcc tctgctcctt ccaaagaaca
     2701 gttagagcag gaaaaacagc tgctgctctc ttttaagcca gtgatgcaga agtttcttca
     2761 tgatcatgtg gatctacagg tcagtgccct gtatgctttg caggtgcact gttacaacag
     2821 cagcttccca aaaggcatgt tacttcgatt ttttgttcat ttctatgaca tggaaattat
     2881 tgaagaggaa gctttcttag cttggaagga agacataact caagagtttc caggaaaagg
     2941 caaggctttg ttccaggtga atcagtggct aacctggcta gaaactgctg aagaagaaga
     3001 atcagaggaa gaagctgact aaagaaccag ccaaagcctt aaattgtgca aaacatactg
     3061 ttgctatgat gtaactgcat ttgacctaac cactgcgaaa attcattccg ctgtaacgtt
     3121 tttcacaata tttaaagcag aagcacgtca gtaaggtttc cttctgcata aggtttttgt
     3181 agtgtgatgt cttaatcata gtctaccatc aaatacttta ggagtatcct taatgtttag
     3241 atagaatatt agcagcatgc aataattaca tcctaagttc tcaagcggag gcagtctatt
     3301 gcaaggacct tctttgctgc cagttaccat aggctgtttt aagttagaaa actgaatagc
     3361 aacactgaat actgtagaga tgcactttgc tcagtaatac ttgagttgtt gcaatatttg
     3421 attatccatt tggttgttac agaaaaattc ttaactgtaa ttgatggttg ttgccgtaat
     3481 agtatattgc ctgtatttct acctctagta atgggcttta tgtgctagat tttaatatcc
     3541 ttgagcctgg gcaagtgcac aagtcttttt aaaagaaaca tggtttactt gcacaaaact
     3601 gatcagtttg agagatcatt aatgcccttg aagtggtttt tgtgggtgtg aaacaaatgg
     3661 tgagaatttg aattggtccc tcttattata gtattgaaat taagtctact taatttatca
     3721 agtcatgttc atgccctgat tttatatact tgtatctatc aataaacatt gtgatacttg
     3781 atgtagttgt acatgacttc aaatagtatc tgcaaagttc tggagaattg ttcactttgt
     3841 tttgaagtat ggatttatct cagttaagaa cttgggaaat ttttaatttc atagtcttaa
     3901 tcagtagtga ttgggcctgt aaagaaacat cccttaattg gaacatttaa gttttttttt
     3961 taccttggga ctgttcctga tcagtctcat tctaatgcaa actgtcaact taatctgacc
     4021 tttaccacca cagaccttgt gtcaactctt tggctctgct ttctaagcac ctaaggaata
     4081 agcttgcctc atccaacaac ctctttactg aggaacccgc ttagccctta ttctccattt
     4141 attcttcaca acaaaacaac cagcacaaga aactgaaaaa caaaactgtg catgctttag
     4201 aacccagcag actaagattg gctccctctc actgcttttc attttttttt cttgcccttg
     4261 cacagtcacc tacttaacct ggaatagtta atggtcccct taccctttaa ccttcaggtg
     4321 tggtgtaaat gtcaaagaac tagccttgct ctactggcac aatgagaagt ttgtatcagt
     4381 caaactgatg caaaagaaaa aaaatgccct ttggcatgga atccagagaa ataacagact
     4441 gggtggtcaa gttctgtgtc ccctgtccaa tatctgttct caggtgtcac cagctcctac
     4501 ttggtgacca gagcctactg taggcagagt agtaacagat aagcccttgc tgcttcctag
     4561 cagaaccatg gtctgccttg gtgactcatg tcatcaagat ttgcagtaga gtagaggaac
     4621 tgggaagagt tgaagtggac agccgaagag gcctgtccca cacctcagcc cagcctggtc
     4681 cactgcactc accccttggt gaaagcagag ctctgagggg ataatgtgat ctctccccag
     4741 tcattgctca gcgggacggt gaatttaagt tttgtgagtt tcaatcttag agagttggaa
     4801 taattagaaa tttaagagca attcaattag gtttaggatc caaattgttc tggataaaag
     4861 attgtctttg aatctgaagc ttgattgact gcatgataac tgagttaggg ctaacagaac
     4921 taactgaaat gatctgtgta taccccactc actagaaaaa ctagtaatga ataaatgctt
     4981 ggatagtgaa aaaaaaaaaa aaaaaaaa
//
