LOCUS       NM_019356               1377 bp    mRNA    linear   ROD 02-APR-2024
DEFINITION  Rattus norvegicus eukaryotic translation initiation factor 2
            subunit alpha (Eif2s1), mRNA.
ACCESSION   NM_019356
VERSION     NM_019356.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 1377)
  AUTHORS   Zheng,W., Wang,K., Wu,Y., Yan,G., Zhang,C., Li,Z., Wang,L. and
            Chen,S.
  TITLE     C9orf72 regulates the unfolded protein response and stress granule
            formation by interacting with eIF2alpha
  JOURNAL   Theranostics 12 (17), 7289-7306 (2022)
   PUBMED   36438488
  REMARK    GeneRIF: C9orf72 regulates the unfolded protein response and stress
            granule formation by interacting with eIF2alpha.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1377)
  AUTHORS   Beauvais,G., Rodriguez-Losada,N., Ying,L., Zakirova,Z.,
            Watson,J.L., Readhead,B., Gadue,P., French,D.L., Ehrlich,M.E. and
            Gonzalez-Alegre,P.
  TITLE     Exploring the Interaction Between eIF2alpha Dysregulation, Acute
            Endoplasmic Reticulum Stress and DYT1 Dystonia in the Mammalian
            Brain
  JOURNAL   Neuroscience 371, 455-468 (2018)
   PUBMED   29289717
  REMARK    GeneRIF: TorsinA was post-transcriptionally upregulated upon acute
            ER stress, suggesting a role in this response. Increased basal
            phosphorylation of eIF2alpha in DYT1 transgenic rats was associated
            with abnormal response to acute ER stress. Unbiased RNA-Seq-based
            transcriptomic analysis of embryonic brain tissue in heterozygous
            and homozygous DYT1 knockin mice confirmed presence of eIF2alpha
            dysregulation in the DYT1 brain.
REFERENCE   3  (bases 1 to 1377)
  AUTHORS   Mahboubi,H., Koromilas,A.E. and Stochaj,U.
  TITLE     AMP Kinase Activation Alters Oxidant-Induced Stress Granule
            Assembly by Modulating Cell Signaling and Microtubule Organization
  JOURNAL   Mol Pharmacol 90 (4), 460-468 (2016)
   PUBMED   27430620
REFERENCE   4  (bases 1 to 1377)
  AUTHORS   Xue,B., Fitzgerald,C.A., Jin,D.Z., Mao,L.M. and Wang,J.Q.
  TITLE     Amphetamine elevates phosphorylation of eukaryotic initiation
            factor 2alpha (eIF2alpha) in the rat forebrain via activating
            dopamine D1 and D2 receptors
  JOURNAL   Brain Res 1646, 459-466 (2016)
   PUBMED   27338925
  REMARK    GeneRIF: results demonstrate the sensitivity of eukaryotic
            initiation factor 2alpha-subunit (eIF2alpha) to stimulant exposure.
            Amphetamine possesses the ability to stimulate eIF2alpha
            phosphorylation in striatal and medial prefrontal cortex neurons in
            vivo in a dopamine D1 and D2 receptor-dependent manner.
REFERENCE   5  (bases 1 to 1377)
  AUTHORS   Wang,A.P., Li,X.H., Yang,Y.M., Li,W.Q., Zhang,W., Hu,C.P., Zhang,Z.
            and Li,Y.J.
  TITLE     A Critical Role of the mTOR/eIF2alpha Pathway in Hypoxia-Induced
            Pulmonary Hypertension
  JOURNAL   PLoS One 10 (6), e0130806 (2015)
   PUBMED   26120832
  REMARK    GeneRIF: These results suggest a critical role of the
            mTOR/eIF2alpha pathway in hypoxic vascular remodeling and pulmonary
            arterial vascular smooth muscle cell proliferation of pulmonary
            hypertension.
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 1377)
  AUTHORS   Chang,G.C., Liu,R., Panniers,R. and Li,G.C.
  TITLE     Rat fibroblasts transfected with the human 70-kDa heat shock gene
            exhibit altered translation and eukaryotic initiation factor 2
            alpha phosphorylation following heat shock
  JOURNAL   Int J Hyperthermia 10 (3), 325-337 (1994)
   PUBMED   7930798
REFERENCE   7  (bases 1 to 1377)
  AUTHORS   Bommer,U.A., Stahl,J., Henske,A., Lutsch,G. and Bielka,H.
  TITLE     Identification of proteins of the 40 S ribosomal subunit involved
            in interaction with initiation factor eIF-2 in the quaternary
            initiation complex by means of monospecific antibodies
  JOURNAL   FEBS Lett 233 (1), 114-118 (1988)
   PUBMED   3384085
REFERENCE   8  (bases 1 to 1377)
  AUTHORS   Bommer,U.A., Lutsch,G., Behlke,J., Stahl,J., Nesytova,N., Henske,A.
            and Bielka,H.
  TITLE     Shape and location of eukaryotic initiation factor eIF-2 on the 40S
            ribosomal subunit of rat liver. Immunoelectron-microscopic and
            hydrodynamic investigations
  JOURNAL   Eur J Biochem 172 (3), 653-662 (1988)
   PUBMED   2450747
REFERENCE   9  (bases 1 to 1377)
  AUTHORS   Colbert,R.A., Hucul,J.A., Scorsone,K.A. and Young,D.A.
  TITLE     Alpha subunit of eukaryotic translational initiation factor-2 is a
            heat-shock protein
  JOURNAL   J Biol Chem 262 (35), 16763-16766 (1987)
   PUBMED   3500171
REFERENCE   10 (bases 1 to 1377)
  AUTHORS   Ernst,H., Duncan,R.F. and Hershey,J.W.
  TITLE     Cloning and sequencing of complementary DNAs encoding the
            alpha-subunit of translational initiation factor eIF-2.
            Characterization of the protein and its messenger RNA
  JOURNAL   J Biol Chem 262 (3), 1206-1212 (1987)
   PUBMED   2948954
COMMENT     PROVISIONAL REFSEQ: This record has not yet been subject to final
            NCBI review. The reference sequence was derived from J02646.1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: J02646.1, BC087019.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMD00132261, SAMD00132262
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
FEATURES             Location/Qualifiers
     source          1..1377
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /db_xref="taxon:10116"
                     /chromosome="6"
                     /map="6q24"
     gene            1..1377
                     /gene="Eif2s1"
                     /note="eukaryotic translation initiation factor 2 subunit
                     alpha"
                     /db_xref="GeneID:54318"
                     /db_xref="RGD:620963"
     exon            1..26
                     /gene="Eif2s1"
                     /inference="alignment:Splign:2.1.0"
     exon            27..268
                     /gene="Eif2s1"
                     /inference="alignment:Splign:2.1.0"
     CDS             28..975
                     /gene="Eif2s1"
                     /note="EIF-2A; EIF-2alpha; eIF-2-alpha; eukaryotic
                     translation initiation factor 2 subunit 1 alpha;
                     eIF2-alpha"
                     /codon_start=1
                     /product="eukaryotic translation initiation factor 2
                     subunit 1"
                     /protein_id="NP_062229.1"
                     /db_xref="GeneID:54318"
                     /db_xref="RGD:620963"
                     /translation="MPGLSCRFYQHKFPEVEDVVMVNVRSIAEMGAYVSLLEYNNIEG
                     MILLSELSRRRIRSINKLIRIGRNECVVVIRVDKEKGYIDLSKRRVSPEEAIKCEDKF
                     TKSKTVYSILRHVAEVLEYTKDEQLESLFQRTAWVFDDKYKRPGYGAYDAFKHAVSDP
                     SILDSLDLNEDEREVLINNINRRLTPQAVKIRADIEVACYGYEGIDAVKEALRAGLNC
                     STETMPIKINLIAPPRYVMTTTTLERTEGLSVLNQAMAVIKEKIEEKRGVFNVQMEPK
                     VVTDTDETELARQLERLERENAEVDGDDDAEEMEAKAED"
     exon            269..348
                     /gene="Eif2s1"
                     /inference="alignment:Splign:2.1.0"
     exon            349..500
                     /gene="Eif2s1"
                     /inference="alignment:Splign:2.1.0"
     exon            501..607
                     /gene="Eif2s1"
                     /inference="alignment:Splign:2.1.0"
     exon            608..705
                     /gene="Eif2s1"
                     /inference="alignment:Splign:2.1.0"
     exon            706..849
                     /gene="Eif2s1"
                     /inference="alignment:Splign:2.1.0"
     exon            850..1377
                     /gene="Eif2s1"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 gttcgggatt cacacataca cttcagaatg ccgggtctaa gttgtagatt ttatcaacac
       61 aaatttcctg aggtcgaaga tgtagtgatg gtgaatgtaa gatccattgc tgaaatgggg
      121 gcctatgtca gcttgttgga atataataac attgaaggca tgattcttct tagtgaatta
      181 tccagacgac gtatccgttc tataaacaaa ctgatccgaa ttggcagaaa tgaatgtgta
      241 gttgtcatta gagtggataa agaaaaagga tatatagatt tgtcaaaaag aagagtttct
      301 ccagaggaag caatcaaatg tgaagacaaa ttcacaaaat ccaaaactgt ttatagcatt
      361 cttcgccatg ttgctgaggt attagagtat accaaggatg agcagctgga aagcctattc
      421 cagaggactg cctgggtctt tgatgacaag tacaagagac ctggatatgg tgcctatgat
      481 gcctttaagc atgcagtctc agacccatct atcttggata gtttagattt gaatgaagat
      541 gaaagagaag tactcattaa caatatcaat aggcgtttga ccccacaagc tgtcaagatt
      601 cgagcagata ttgaggtagc ttgctatggt tacgaaggca ttgatgctgt aaaagaagcc
      661 ctgagagcag gtttgaattg ttctacagaa accatgccca tcaagattaa tctaatagct
      721 ccacccaggt atgtgatgac aacaacgacc ctagagagga cagaaggact ctctgttctc
      781 aatcaggcta tggcagtcat caaagaaaag attgaggaga agaggggagt gttcaatgtt
      841 cagatggagc ccaaagtggt tacagataca gatgagactg aacttgcaag gcagctggaa
      901 cggcttgaga gagaaaatgc agaagtggat ggagatgatg atgcagaaga aatggaagcc
      961 aaagctgaag attaaccttt tggaaaacag tccaatttaa ggagtacgaa gcagcccttt
     1021 ctggctgtaa accctagact tgaaagtttt ccagtattga aaacttcaaa gctgaatatt
     1081 tttatttcca agtatttaag tattcgacaa gccagaatct aaatgccctc cttcatgtca
     1141 gctgttttca catagtggct ctaacacctc aagcgttttt aagggagtgg cttgatttga
     1201 ccagagacaa atgttaaacc gcagtcctaa aattgggctt gcggttttca tttctgatgt
     1261 ctctggattg gcacccttat ggtttagaga attaccaggg gctccagaca ccaacaatcc
     1321 caacctttct atataaaatg tactcaagca aacatcaaat aaatttctgg gatattt
//
