LOCUS       NM_138866               3147 bp    mRNA    linear   ROD 03-APR-2024
DEFINITION  Rattus norvegicus eukaryotic translation initiation factor 2B
            subunit epsilon (Eif2b5), mRNA.
ACCESSION   NM_138866
VERSION     NM_138866.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 3147)
  AUTHORS   Kashiwagi,K., Shigeta,T., Imataka,H., Ito,T. and Yokoyama,S.
  TITLE     Expression, purification, and crystallization of
            Schizosaccharomyces pombe eIF2B
  JOURNAL   J Struct Funct Genomics 17 (1), 33-38 (2016)
   PUBMED   27023709
REFERENCE   2  (bases 1 to 3147)
  AUTHORS   Hinnebusch,A.G. and Lorsch,J.R.
  TITLE     The mechanism of eukaryotic translation initiation: new insights
            and challenges
  JOURNAL   Cold Spring Harb Perspect Biol 4 (10), a011544 (2012)
   PUBMED   22815232
  REMARK    Review article
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 3147)
  AUTHORS   Tuckow,A.P., Vary,T.C., Kimball,S.R. and Jefferson,L.S.
  TITLE     Ectopic expression of eIF2Bepsilon in rat skeletal muscle rescues
            the sepsis-induced reduction in guanine nucleotide exchange
            activity and protein synthesis
  JOURNAL   Am J Physiol Endocrinol Metab 299 (2), E241-E248 (2010)
   PUBMED   20484009
  REMARK    GeneRIF: modulation of eIF2Bepsilon expression may be sufficient to
            correct deficits in skeletal muscle protein synthesis associated
            with sepsis and other muscle-wasting conditions
REFERENCE   4  (bases 1 to 3147)
  AUTHORS   Kantor,L., Pinchasi,D., Mintz,M., Hathout,Y., Vanderver,A. and
            Elroy-Stein,O.
  TITLE     A point mutation in translation initiation factor 2B leads to a
            continuous hyper stress state in oligodendroglial-derived cells
  JOURNAL   PLoS One 3 (11), e3783 (2008)
   PUBMED   19023445
  REMARK    GeneRIF: oligodendroglial-derived cells expressing a mutated eIF2B5
            constantly use their stress response mechanism as an adaptation
            mean in order to survive
REFERENCE   5  (bases 1 to 3147)
  AUTHORS   Kubica,N., Crispino,J.L., Gallagher,J.W., Kimball,S.R. and
            Jefferson,L.S.
  TITLE     Activation of the mammalian target of rapamycin complex 1 is both
            necessary and sufficient to stimulate eukaryotic initiation factor
            2Bvarepsilon mRNA translation and protein synthesis
  JOURNAL   Int J Biochem Cell Biol 40 (11), 2522-2533 (2008)
   PUBMED   18556237
  REMARK    GeneRIF: Data show that activation of mTORC1 is necessary and
            sufficient to stimulate eIF2Bepsilon mRNA translation.
REFERENCE   6  (bases 1 to 3147)
  AUTHORS   Fabian,J.R., Kimball,S.R., Heinzinger,N.K. and Jefferson,L.S.
  TITLE     Subunit assembly and guanine nucleotide exchange activity of
            eukaryotic initiation factor-2B expressed in Sf9 cells
  JOURNAL   J Biol Chem 272 (19), 12359-12365 (1997)
   PUBMED   9139680
REFERENCE   7  (bases 1 to 3147)
  AUTHORS   Flowers,K.M., Mellor,H., Matts,R.L., Kimball,S.R. and
            Jefferson,L.S.
  TITLE     Cloning and characterization of complementary and genomic DNAs
            encoding the epsilon-subunit of rat translation initiation
            factor-2B
  JOURNAL   Biochim Biophys Acta 1307 (3), 318-324 (1996)
   PUBMED   8688467
REFERENCE   8  (bases 1 to 3147)
  AUTHORS   Welsh,G.I., Miyamoto,S., Price,N.T., Safer,B. and Proud,C.G.
  TITLE     T-cell activation leads to rapid stimulation of translation
            initiation factor eIF2B and inactivation of glycogen synthase
            kinase-3
  JOURNAL   J Biol Chem 271 (19), 11410-11413 (1996)
   PUBMED   8626696
REFERENCE   9  (bases 1 to 3147)
  AUTHORS   Gilligan,M., Welsh,G.I., Flynn,A., Bujalska,I., Diggle,T.A.,
            Denton,R.M., Proud,C.G. and Docherty,K.
  TITLE     Glucose stimulates the activity of the guanine nucleotide-exchange
            factor eIF-2B in isolated rat islets of Langerhans
  JOURNAL   J Biol Chem 271 (4), 2121-2125 (1996)
   PUBMED   8567668
REFERENCE   10 (bases 1 to 3147)
  AUTHORS   Karinch,A.M., Kimball,S.R., Vary,T.C. and Jefferson,L.S.
  TITLE     Regulation of eukaryotic initiation factor-2B activity in muscle of
            diabetic rats
  JOURNAL   Am J Physiol 264 (1 Pt 1), E101-E108 (1993)
   PUBMED   8430778
COMMENT     PROVISIONAL REFSEQ: This record has not yet been subject to final
            NCBI review. The reference sequence was derived from BC085698.1.
            
            On Nov 15, 2004 this sequence version replaced NM_138866.1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC085698.1, U19516.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA5760389 [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
FEATURES             Location/Qualifiers
     source          1..3147
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /db_xref="taxon:10116"
                     /chromosome="11"
                     /map="11q23"
     gene            1..3147
                     /gene="Eif2b5"
                     /note="eukaryotic translation initiation factor 2B subunit
                     epsilon"
                     /db_xref="GeneID:192234"
                     /db_xref="RGD:708380"
     exon            1..216
                     /gene="Eif2b5"
                     /inference="alignment:Splign:2.1.0"
     CDS             37..2187
                     /gene="Eif2b5"
                     /note="translation initiation factor eIF-2B subunit
                     epsilon; eIF-2B GDP-GTP exchange factor subunit epsilon;
                     initiation factor eIF-2Be; eukaryotic translation
                     initiation factor 2B subunit 5 epsilon; eIF2B GDP-GTP
                     exchange factor subunit epsilon"
                     /codon_start=1
                     /product="translation initiation factor eIF2B subunit
                     epsilon"
                     /protein_id="NP_620221.2"
                     /db_xref="GeneID:192234"
                     /db_xref="RGD:708380"
                     /translation="MAATAAVPSAVGGRANKRGGGSGGGGTQGAEEEPPPPLQAVLVA
                     DSFDRRFFPISKDQPRVLLPLANVALIDYTLEFLTATGVQETFVFCCWKAAQIKEHLQ
                     KSKWCHPTSLNVVRIITSDLYRSLGDVLRDVDAKALVRSDFLLIYGDVVSNINISKAL
                     EEHRLRRKLEKNVSVMTMVFKESSPSHPTRCHEDNVVLAVDSTTNRILHFQKTQGLRH
                     FSFPLGLFQGSLDGVEIRYDLLDCHISICSPQVAQLFTDNFDYQTRDDFVRGLLVNEE
                     ILGNQIHLHVTSREYGARVSNLHMYSAVCADVIRRWVYPLTPEVNFTDSSTQSYTHSR
                     HNIYRGPEVSLGHGSVLEENVLLGAGTVVGSNCSITNSVIGPNCHIGDNVVLDQAYLW
                     QGVRVAAGAQIHQSLLCDRAEVKERVILKPHCVLTSQVVVGPDIILPEGSVISLHPPD
                     AEEDEDDGQFSDDSGADQEKEKVKLKGYNPAEVGPEGQGYLWKAEDVDEKEDEELRQS
                     LWGLMINMEEESETESERSVDPEELDSRAGSPQLDDIRVFQNEVLGTLQRGREENISC
                     DNLVLEINSLKYAYNISLKEVMQVLSHVVLEFPLQQVDGVLDPNRYCALLLPLLKAWS
                     PVFRNYIKRAADHLEALAAIEDFFLEHETLVPSLAKVLMAFYQLEILAEETILSWFSQ
                     RDITDKGQQLRKNQQLQRFIQWLREAEEESSDDD"
     exon            217..341
                     /gene="Eif2b5"
                     /inference="alignment:Splign:2.1.0"
     exon            342..527
                     /gene="Eif2b5"
                     /inference="alignment:Splign:2.1.0"
     exon            528..705
                     /gene="Eif2b5"
                     /inference="alignment:Splign:2.1.0"
     exon            706..786
                     /gene="Eif2b5"
                     /inference="alignment:Splign:2.1.0"
     exon            787..864
                     /gene="Eif2b5"
                     /inference="alignment:Splign:2.1.0"
     exon            865..1177
                     /gene="Eif2b5"
                     /inference="alignment:Splign:2.1.0"
     exon            1178..1323
                     /gene="Eif2b5"
                     /inference="alignment:Splign:2.1.0"
     exon            1324..1465
                     /gene="Eif2b5"
                     /inference="alignment:Splign:2.1.0"
     exon            1466..1567
                     /gene="Eif2b5"
                     /inference="alignment:Splign:2.1.0"
     exon            1568..1675
                     /gene="Eif2b5"
                     /inference="alignment:Splign:2.1.0"
     exon            1676..1766
                     /gene="Eif2b5"
                     /inference="alignment:Splign:2.1.0"
     exon            1767..1890
                     /gene="Eif2b5"
                     /inference="alignment:Splign:2.1.0"
     exon            1891..2016
                     /gene="Eif2b5"
                     /inference="alignment:Splign:2.1.0"
     exon            2017..2127
                     /gene="Eif2b5"
                     /inference="alignment:Splign:2.1.0"
     exon            2128..3114
                     /gene="Eif2b5"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 gtggcttccc ggcggacgtg ctgactgcga agcaaaatgg cagccactgc ggcggttccc
       61 agcgctgtag gcggccgagc gaacaaacgc ggcggcggct ccggcggcgg tggaacccaa
      121 ggtgcggagg aggagccgcc gccgcccctc caggcagttt tagtggccga tagcttcgac
      181 cgccgcttct tccccatctc caaggaccag cctcgggtcc tattgcccct ggctaatgtc
      241 gcgctgatcg actatactct ggaattcttg actgccacgg gtgtacagga aacatttgtc
      301 ttttgctgct ggaaggctgc tcagatcaaa gaacacttac agaaatccaa atggtgccat
      361 cctacgtccc tcaatgttgt tcgaataatc acatcagacc tgtaccgatc actaggagat
      421 gtcctccgtg atgtcgatgc caaggccttg gtgcgctctg atttcctcct gatatatgga
      481 gatgtcgtct caaatatcaa tatttccaaa gccctggagg aacacaggtt aagaaggaag
      541 ctagaaaaaa atgtgtctgt catgacaatg gtgttcaaag agtcctcacc cagtcaccct
      601 acacgctgcc atgaggacaa tgtggtgcta gctgtggata gcaccacaaa caggattctt
      661 cacttccaga agacccaagg cctccgccat ttctcctttc ctttgggcct attccagggc
      721 agtctagacg gagtggagat tcgatatgac ttgttggatt gtcacatcag catctgctct
      781 cctcaggtag ctcagctctt cacagacaac tttgactacc aaactcgaga tgattttgtg
      841 cgaggcctgc tagtgaatga ggagatccta ggaaatcaga ttcacttgca tgtgacaagt
      901 agggaatatg gtgcccgggt ctccaaccta cacatgtact cagctgtctg tgctgacgtc
      961 atccgccgat gggtctaccc tcttactcca gaagtcaact tcactgacag cagtacacaa
     1021 agctacactc attcccgaca caacatctac cgagggcctg aagtcagcct gggccacggc
     1081 agcgtcctgg aggaaaacgt gcttctggga gctggcactg tcgttggcag caactgctcc
     1141 atcaccaaca gtgtcattgg ccctaactgc cacattggtg ataatgtggt actggaccag
     1201 gcctacctgt ggcagggagt tcgagtggct gctggagcac aaatacacca gtctctgctc
     1261 tgtgacagag ctgaggtcaa ggagcgagtc atactgaagc cacactgtgt cctcacttcc
     1321 caggtggtag tgggcccaga catcatactg cccgagggct cggtgatctc tttgcaccct
     1381 ccagatgcag aggaagatga agatgatggc cagtttagtg atgattctgg cgctgaccaa
     1441 gaaaaggaga aagtgaagct gaaaggttac aatccggcag aagttggccc tgaaggccag
     1501 ggatacctct ggaaagctga ggatgtggat gagaaggaag atgaagagct gcggcagagt
     1561 ctctggggac tcatgatcaa tatggaggag gaaagtgaga cggaaagtga gcggagcgtg
     1621 gatcccgagg agctggacag ccgagcaggc tcccctcagc tggatgacat cagagttttc
     1681 cagaacgagg tcttaggaac gctgcagcgg ggcagagagg agaacatctc ctgtgacaat
     1741 ctagtcctgg agatcaactc cctcaagtat gcctacaaca ttagcctaaa ggaagtgatg
     1801 caggtcctga gccatgtggt cctggagttc cccctgcaac aagtagatgg cgtgcttgac
     1861 ccaaaccgct actgtgccct gctgcttcct ctgctcaaag cctggagccc tgtttttcgg
     1921 aactacataa agcgcgcagc tgaccatttg gaagcattgg cggccattga ggacttcttc
     1981 ttggaacatg aaactcttgt tccttccctg gccaaggtcc tgatggcttt ctaccagctg
     2041 gagatcttgg ctgaggaaac aatcttgagc tggttcagcc aaagagacat aactgacaaa
     2101 ggccagcagt tacgtaagaa tcaacagctg cagaggttca tccagtggct gagagaggca
     2161 gaagaggagt catctgacga tgactgatgg tgactgccgg atccatgcat ctcattggct
     2221 gtcctagcag atagggcaaa acaagaaagc ttgctacaga agcatgagcg gccactacag
     2281 actgagaatt aatggaacgg aagttgcaac tacagtattc ttcctactgc tgggaaccat
     2341 gtgcttctcg tcctgactga agtaggatga aggaagttag actgaactag gggaaatata
     2401 gacagaccct gcagttgggg gttggccaga ggaagcactt tgtgtttcag ctttccctca
     2461 gggagcagta aagagcagct ggtactccct gttgtttgta tttgctgata ttataaagag
     2521 gagtgtacgg gcttctctcc tcatcatcag ccaggtaagg acaaatatgc actcactagg
     2581 ctgcttttag ccaacatctg gaggagaggg agaagggtcg aggtggccac gaggcggtgc
     2641 tatgctaatt gactccatcc cctggatcat gggacatcag tggccttcct ccaaagacca
     2701 gtctgtaacc ctgacgtgct gccagagtcc atttccatag ctttccctgg tctgagcatt
     2761 cttctgccca agggcgaggg taccaggcat atctgaagcc atctgctgtt cctcttgcct
     2821 tttccaggtg gcccgcttct ggttctgtca gacactgggc cacattcatc ttagaggata
     2881 aggaaatgca tccagtgtgg tagtacacat ctttaatctc agtaccctgg atggaagaga
     2941 tagacagatc tctgagttca aggccagcct ggtctataag agttcctaaa cagtgagtgc
     3001 tgcacagaga aaccctttgt gtatacgtgg gagacagagg agaggtggat taaggaatgc
     3061 tcctgagatg ttatgaatct tgcacggcat aattaagaac cttatagaac tctgaaaaaa
     3121 aaaaaaaaaa aaaaaaaaaa aaaaaaa
//
