LOCUS       NM_053950               1634 bp    mRNA    linear   ROD 03-APR-2024
DEFINITION  Rattus norvegicus eukaryotic translation initiation factor 2B
            subunit delta (Eif2b4), mRNA.
ACCESSION   NM_053950
VERSION     NM_053950.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 1634)
  AUTHORS   Kashiwagi,K., Shigeta,T., Imataka,H., Ito,T. and Yokoyama,S.
  TITLE     Expression, purification, and crystallization of
            Schizosaccharomyces pombe eIF2B
  JOURNAL   J Struct Funct Genomics 17 (1), 33-38 (2016)
   PUBMED   27023709
REFERENCE   2  (bases 1 to 1634)
  AUTHORS   Hinnebusch,A.G. and Lorsch,J.R.
  TITLE     The mechanism of eukaryotic translation initiation: new insights
            and challenges
  JOURNAL   Cold Spring Harb Perspect Biol 4 (10), a011544 (2012)
   PUBMED   22815232
  REMARK    Review article
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 1634)
  AUTHORS   Mikami,S., Masutani,M., Sonenberg,N., Yokoyama,S. and Imataka,H.
  TITLE     An efficient mammalian cell-free translation system supplemented
            with translation factors
  JOURNAL   Protein Expr Purif 46 (2), 348-357 (2006)
   PUBMED   16289705
REFERENCE   4  (bases 1 to 1634)
  AUTHORS   Fogli,A., Gauthier-Barichard,F., Schiffmann,R., Vanderhoof,V.H.,
            Bakalov,V.K., Nelson,L.M. and Boespflug-Tanguy,O.
  TITLE     Screening for known mutations in EIF2B genes in a large panel of
            patients with premature ovarian failure
  JOURNAL   BMC Womens Health 4 (1), 8 (2004)
   PUBMED   15507143
  REMARK    Publication Status: Online-Only
REFERENCE   5  (bases 1 to 1634)
  AUTHORS   Van Haren,K., van der Voorn,J.P., Peterson,D.R., van der Knaap,M.S.
            and Powers,J.M.
  TITLE     The life and death of oligodendrocytes in vanishing white matter
            disease
  JOURNAL   J Neuropathol Exp Neurol 63 (6), 618-630 (2004)
   PUBMED   15217090
REFERENCE   6  (bases 1 to 1634)
  AUTHORS   Fabian,J.R., Kimball,S.R., Heinzinger,N.K. and Jefferson,L.S.
  TITLE     Subunit assembly and guanine nucleotide exchange activity of
            eukaryotic initiation factor-2B expressed in Sf9 cells
  JOURNAL   J Biol Chem 272 (19), 12359-12365 (1997)
   PUBMED   9139680
REFERENCE   7  (bases 1 to 1634)
  AUTHORS   Price,N.T., Mellor,H., Craddock,B.L., Flowers,K.M., Kimball,S.R.,
            Wilmer,T., Jefferson,L.S. and Proud,C.G.
  TITLE     eIF2B, the guanine nucleotide-exchange factor for eukaryotic
            initiation factor 2. Sequence conservation between the alpha, beta
            and delta subunits of eIF2B from mammals and yeast
  JOURNAL   Biochem J 318 (Pt 2) (Pt 2), 637-643 (1996)
   PUBMED   8929216
REFERENCE   8  (bases 1 to 1634)
  AUTHORS   Welsh,G.I., Miyamoto,S., Price,N.T., Safer,B. and Proud,C.G.
  TITLE     T-cell activation leads to rapid stimulation of translation
            initiation factor eIF2B and inactivation of glycogen synthase
            kinase-3
  JOURNAL   J Biol Chem 271 (19), 11410-11413 (1996)
   PUBMED   8626696
REFERENCE   9  (bases 1 to 1634)
  AUTHORS   Gilligan,M., Welsh,G.I., Flynn,A., Bujalska,I., Diggle,T.A.,
            Denton,R.M., Proud,C.G. and Docherty,K.
  TITLE     Glucose stimulates the activity of the guanine nucleotide-exchange
            factor eIF-2B in isolated rat islets of Langerhans
  JOURNAL   J Biol Chem 271 (4), 2121-2125 (1996)
   PUBMED   8567668
REFERENCE   10 (bases 1 to 1634)
  AUTHORS   Karinch,A.M., Kimball,S.R., Vary,T.C. and Jefferson,L.S.
  TITLE     Regulation of eukaryotic initiation factor-2B activity in muscle of
            diabetic rats
  JOURNAL   Am J Physiol 264 (1 Pt 1), E101-E108 (1993)
   PUBMED   8430778
COMMENT     PROVISIONAL REFSEQ: This record has not yet been subject to final
            NCBI review. The reference sequence was derived from Z48225.1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: Z48225.1, SRR8487226.189543.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMD00132261, SAMD00132262
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
FEATURES             Location/Qualifiers
     source          1..1634
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="Sprague-Dawley"
                     /db_xref="taxon:10116"
                     /chromosome="6"
                     /map="6q14"
     gene            1..1634
                     /gene="Eif2b4"
                     /gene_synonym="Eif2b"
                     /note="eukaryotic translation initiation factor 2B subunit
                     delta"
                     /db_xref="GeneID:117019"
                     /db_xref="RGD:620208"
     exon            1..38
                     /gene="Eif2b4"
                     /gene_synonym="Eif2b"
                     /inference="alignment:Splign:2.1.0"
     CDS             8..1582
                     /gene="Eif2b4"
                     /gene_synonym="Eif2b"
                     /function="guanine nucleotide exchange factor"
                     /note="translation initiation factor eIF-2B subunit delta;
                     eIF-2B GDP-GTP exchange factor subunit delta; eukaryotic
                     translation initiation factor 2B, subunit 4 delta;
                     eukaryotic translation initiation factor 2B subunit 4;
                     eIF2B GDP-GTP exchange factor subunit delta"
                     /codon_start=1
                     /product="translation initiation factor eIF2B subunit
                     delta"
                     /protein_id="NP_446402.1"
                     /db_xref="GeneID:117019"
                     /db_xref="RGD:620208"
                     /translation="MAAVAVAVREESRSEMKTELSPRPGAAGRELTQEEKLQLRKEKK
                     QQKKKRKEEKGADQEIGSAVSAAQRQDPVRELQGTGSQLGGTTGEKLPAGRSKAELRA
                     ERRAKQEAERALKQARKGEQGGPSPQACPSTAGEATSGVKRVPEHTQADDPTLLRRLL
                     RKPDRQQVPTRKDYGSKVSLFSHLPQYSRQSSLTQYMSIPSSVIHPAMVRLGLQYSQG
                     LVSGSNARCIALLHALQQVIQDYTTPPNEELSRDLVNKLKPYISFLTQCRPMSASMCN
                     AIKFFNKEVTGMSSSKREEEAKSELKEAIDRYVQEKIVLASQAISRFASKKISDGDVI
                     LVYGCSSLVSRILQEAWVEGRRFRVVVVDSRPRLEGRHMLHCLVRAGVPTSYLLIPAA
                     SYVLPEVSKVLLGAHALLANGSVMSRVGTAQLALVARAHNVPVLVCCETYKFCERVQT
                     DAFVSNELDDPDDLQCKRGDQVTLANWQNNSSLRLLNLVYDVTPPELVDLVITELGMI
                     PCSSVPVVLRVKSSDQ"
     exon            39..82
                     /gene="Eif2b4"
                     /gene_synonym="Eif2b"
                     /inference="alignment:Splign:2.1.0"
     exon            83..218
                     /gene="Eif2b4"
                     /gene_synonym="Eif2b"
                     /inference="alignment:Splign:2.1.0"
     exon            219..425
                     /gene="Eif2b4"
                     /gene_synonym="Eif2b"
                     /inference="alignment:Splign:2.1.0"
     exon            426..508
                     /gene="Eif2b4"
                     /gene_synonym="Eif2b"
                     /inference="alignment:Splign:2.1.0"
     exon            509..600
                     /gene="Eif2b4"
                     /gene_synonym="Eif2b"
                     /inference="alignment:Splign:2.1.0"
     exon            601..715
                     /gene="Eif2b4"
                     /gene_synonym="Eif2b"
                     /inference="alignment:Splign:2.1.0"
     exon            716..792
                     /gene="Eif2b4"
                     /gene_synonym="Eif2b"
                     /inference="alignment:Splign:2.1.0"
     exon            793..895
                     /gene="Eif2b4"
                     /gene_synonym="Eif2b"
                     /inference="alignment:Splign:2.1.0"
     exon            896..1023
                     /gene="Eif2b4"
                     /gene_synonym="Eif2b"
                     /inference="alignment:Splign:2.1.0"
     exon            1024..1201
                     /gene="Eif2b4"
                     /gene_synonym="Eif2b"
                     /inference="alignment:Splign:2.1.0"
     exon            1202..1382
                     /gene="Eif2b4"
                     /gene_synonym="Eif2b"
                     /inference="alignment:Splign:2.1.0"
     exon            1383..1626
                     /gene="Eif2b4"
                     /gene_synonym="Eif2b"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 cacagtcatg gctgcggtgg cggtggctgt tcgcgaagaa tcgagatccg agatgaagac
       61 agaactttca cctcggcccg gggcagcggg gcgggagttg acccaagaag agaagcttca
      121 gcttcggaaa gaaaagaaac agcagaagaa gaaacggaag gaggaaaagg gggcagacca
      181 agaaattggc tctgctgtat ctgcagctca acgtcaagac ccagtcagag aacttcaagg
      241 aactggtagt cagttgggag gcactactgg ggagaaactt ccagctggcc ggagtaaggc
      301 agaacttcga gcagaaagga gagccaagca ggaggcagag cgggccctga aacaggccag
      361 aaaaggggaa caaggaggac cctctcctca ggcctgcccc agcacagctg gagaagccac
      421 ctcaggagtg aagcgtgtcc ctgagcacac ccaggctgat gaccccacac ttctgaggag
      481 gctccttaga aagccagatc gacaacaggt tcctacaaga aaggattatg gatccaaagt
      541 cagtctcttc tcccacctac cccagtacag cagacaaagc tccttaaccc agtacatgag
      601 catcccatcc tctgtgatcc acccagccat ggtgcgactc ggtctgcagt actcccaggg
      661 ccttgtcagt ggctccaatg cccggtgcat agcgctgctc cacgctctgc agcaggtgat
      721 tcaggattac acaacacctc ccaatgagga actctccagg gatcttgtaa ataaactaaa
      781 accctacatc agcttcctga cccagtgccg ccccatgtcg gccagcatgt gtaacgccat
      841 caagttcttt aacaaggaag tcactggtat gagcagctcc aagcgggaag aagaggccaa
      901 gtcagaactt aaagaagcca tcgatcggta tgtgcaagag aagattgtgc ttgcatctca
      961 ggcaatttca cgatttgctt ctaagaagat cagtgatggg gacgtgatcc tagtatatgg
     1021 atgctcatct ctggtgtcga gaattctcca ggaggcctgg gttgagggca ggcgcttccg
     1081 ggtggtggtg gtagacagcc ggccccggct ggagggaagg catatgctcc actgtctggt
     1141 ccgtgctggg gtccctacct cctatctgct gattcctgcg gcctcctatg tgctcccaga
     1201 ggtttctaag gtgctattgg gagctcatgc actcctggcc aatggatctg tgatgtcgag
     1261 ggtagggaca gcacagttgg ccctggtggc ccgagctcat aatgttccag tactggtctg
     1321 ctgtgaaaca tacaagttct gtgaacgcgt gcagaccgat gcctttgtct ccaacgagct
     1381 agatgaccct gacgatctcc agtgtaagcg gggagaccag gtgaccctgg cgaactggca
     1441 gaacaactca tcactccggt tgttgaatct ggtctatgac gtgactcccc ccgagcttgt
     1501 ggatctggtg atcacagagt tgggcatgat cccttgcagt tctgtgcctg ttgtcctccg
     1561 agtcaagagt agtgaccaat gaaaggcatc aagggtcaat aaaaaactta ttccttactg
     1621 ccataaaaaa aaaa
//
