LOCUS       NM_001105744            5225 bp    mRNA    linear   ROD 12-NOV-2024
DEFINITION  Rattus norvegicus eukaryotic translation initiation factor 2 alpha
            kinase 4 (Eif2ak4), mRNA.
ACCESSION   NM_001105744 XM_001074965 XM_230462
VERSION     NM_001105744.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 5225)
  AUTHORS   Zhu,M.M., Dai,J., Dai,Z., Peng,Y. and Zhao,Y.Y.
  TITLE     GCN2 kinase activation mediates pulmonary vascular remodeling and
            pulmonary arterial hypertension
  JOURNAL   JCI Insight 9 (20), e177926 (2024)
   PUBMED   39316438
  REMARK    GeneRIF: GCN2 kinase activation mediates pulmonary vascular
            remodeling and pulmonary arterial hypertension.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5225)
  AUTHORS   Bignard,J., Atassi,F., Claude,O., Ghigna,M.R., Mougenot,N.,
            Abdoulkarim,B.S., Deknuydt,F., Gestin,A., Monceau,V., Montani,D.,
            Nadaud,S., Soubrier,F. and Perros,F.
  TITLE     T-cell dysregulation and inflammatory process in Gcn2
            (Eif2ak4-/-)-deficient rats in basal and stress conditions
  JOURNAL   Am J Physiol Lung Cell Mol Physiol 324 (5), L609-L624 (2023)
   PUBMED   36852942
  REMARK    GeneRIF: T-cell dysregulation and inflammatory process in Gcn2
            (Eif2ak4[-/-])-deficient rats in basal and stress conditions.
REFERENCE   3  (bases 1 to 5225)
  AUTHORS   Santos-Ribeiro,D., Lecocq,M., de Beukelaer,M., Verleden,S.,
            Bouzin,C., Ambroise,J., Dorfmuller,P., Yakoub,Y., Huaux,F.,
            Quarck,R., Karmouty-Quintana,H., Ghigna,M.R., Bignard,J.,
            Nadaud,S., Soubrier,F., Horman,S., Perros,F., Godinas,L. and
            Pilette,C.
  TITLE     Disruption of GCN2 Pathway Aggravates Vascular and Parenchymal
            Remodeling during Pulmonary Fibrosis
  JOURNAL   Am J Respir Cell Mol Biol 68 (3), 326-338 (2023)
   PUBMED   36476191
  REMARK    GeneRIF: Disruption of GCN2 Pathway Aggravates Vascular and
            Parenchymal Remodeling during Pulmonary Fibrosis.
REFERENCE   4  (bases 1 to 5225)
  AUTHORS   Manaud,G., Nossent,E.J., Lambert,M., Ghigna,M.R., Boet,A.,
            Vinhas,M.C., Ranchoux,B., Dumas,S.J., Courboulin,A., Girerd,B.,
            Soubrier,F., Bignard,J., Claude,O., Lecerf,F., Hautefort,A.,
            Florio,M., Sun,B., Nadaud,S., Verleden,S.E., Remy,S., Anegon,I.,
            Bogaard,H.J., Mercier,O., Fadel,E., Simonneau,G., Vonk
            Noordegraaf,A., Grunberg,K., Humbert,M., Montani,D., Dorfmuller,P.,
            Antigny,F. and Perros,F.
  TITLE     Comparison of Human and Experimental Pulmonary Veno-Occlusive
            Disease
  JOURNAL   Am J Respir Cell Mol Biol 63 (1), 118-131 (2020)
   PUBMED   32209028
REFERENCE   5  (bases 1 to 5225)
  AUTHORS   Lehman,S.L., Cerniglia,G.J., Johannes,G.J., Ye,J., Ryeom,S. and
            Koumenis,C.
  TITLE     Translational Upregulation of an Individual p21Cip1 Transcript
            Variant by GCN2 Regulates Cell Proliferation and Survival under
            Nutrient Stress
  JOURNAL   PLoS Genet 11 (6), e1005212 (2015)
   PUBMED   26102367
  REMARK    Publication Status: Online-Only
REFERENCE   6  (bases 1 to 5225)
  AUTHORS   Shultz,M.A., Zhang,L., Gu,Y.Z., Baker,G.L., Fannuchi,M.V.,
            Padua,A.M., Gurske,W.A., Morin,D., Penn,S.G., Jovanovich,S.B.,
            Plopper,C.G. and Buckpitt,A.R.
  TITLE     Gene expression analysis in response to lung toxicants: I.
            Sequencing and microarray development
  JOURNAL   Am J Respir Cell Mol Biol 30 (3), 296-310 (2004)
   PUBMED   12947022
REFERENCE   7  (bases 1 to 5225)
  AUTHORS   Deng,J., Harding,H.P., Raught,B., Gingras,A.C., Berlanga,J.J.,
            Scheuner,D., Kaufman,R.J., Ron,D. and Sonenberg,N.
  TITLE     Activation of GCN2 in UV-irradiated cells inhibits translation
  JOURNAL   Curr Biol 12 (15), 1279-1286 (2002)
   PUBMED   12176355
REFERENCE   8  (bases 1 to 5225)
  AUTHORS   Harding,H.P., Novoa,I., Zhang,Y., Zeng,H., Wek,R., Schapira,M. and
            Ron,D.
  TITLE     Regulated translation initiation controls stress-induced gene
            expression in mammalian cells
  JOURNAL   Mol Cell 6 (5), 1099-1108 (2000)
   PUBMED   11106749
REFERENCE   9  (bases 1 to 5225)
  AUTHORS   Sood,R., Porter,A.C., Olsen,D.A., Cavener,D.R. and Wek,R.C.
  TITLE     A mammalian homologue of GCN2 protein kinase important for
            translational control by phosphorylation of eukaryotic initiation
            factor-2alpha
  JOURNAL   Genetics 154 (2), 787-801 (2000)
   PUBMED   10655230
REFERENCE   10 (bases 1 to 5225)
  AUTHORS   Berlanga,J.J., Santoyo,J. and De Haro,C.
  TITLE     Characterization of a mammalian homolog of the GCN2 eukaryotic
            initiation factor 2alpha kinase
  JOURNAL   Eur J Biochem 265 (2), 754-762 (1999)
   PUBMED   10504407
COMMENT     PROVISIONAL REFSEQ: This record has not yet been subject to final
            NCBI review. The reference sequence was derived from CH473949.1.
            
            On Oct 20, 2010 this sequence version replaced NM_001105744.1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            RNAseq introns :: single sample supports all introns SAMD00132283,
                              SAMD00132290 [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
FEATURES             Location/Qualifiers
     source          1..5225
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /db_xref="taxon:10116"
                     /chromosome="3"
                     /map="3q35"
     gene            1..5225
                     /gene="Eif2ak4"
                     /note="eukaryotic translation initiation factor 2 alpha
                     kinase 4"
                     /db_xref="GeneID:114859"
                     /db_xref="RGD:1311439"
     exon            1..236
                     /gene="Eif2ak4"
                     /inference="alignment:Splign:2.1.0"
     CDS             93..5042
                     /gene="Eif2ak4"
                     /EC_number="2.7.11.1"
                     /note="GCN2-like protein"
                     /codon_start=1
                     /product="eIF-2-alpha kinase GCN2"
                     /protein_id="NP_001099214.2"
                     /db_xref="GeneID:114859"
                     /db_xref="RGD:1311439"
                     /translation="MAGGRGAAGRGPAEPQESYSQRQDHELQALEAIYGSDFQDLRPD
                     ARGRVREPPEINLVLYPQGLAGEEVYVQVELRVKCPPTYPDVVPEIELKNTKGLSNES
                     VNLLKSHLEELAKKQCGEVMIFELAHHVQSFLSEHNKPPPKSFHEEMLERQAQEKQQR
                     LLEARQKEEQEQREILHEIQKRKEEIKEEKKRKEMAKQERLEITSLTNQDHASKRDPA
                     GHRAAAILHGGSPDFVGNGKARAHSSGRSRRERQYSVCSGEASPGSCDILYFCVGSAD
                     QLMVHKGKCVGSDEQLGKVVYNALETATGSFVLLYEWVLQWQKKMGPCLTSQEKEKID
                     KCKKQIQGAETEFSSLVKLSHPNIVRYFAMNSREEKDSIVVDILAEHISGISLAAHLS
                     HSGPVPMHQLRKYTAQLLAGLDYLHRNSVVHKVLSTASVLVDAEGTVKITDYSISKRL
                     ADICKEDVFEQTRVRFSDSALPYKTGKKGDVWRLGLLLLSLSQGQECEEYPVTIPSDL
                     PADFQDFLKKCVCLDDKERWSPQQLLKHSFINPQPKMPLVEQSPEDSGGQDYIETIIP
                     SNQLPSAAFFTETQRQFSRYFIEFEELQLLGKGAFGAVIKVQNKLDGCCYAVKRIPIN
                     PASRQFRRIKGEVTLLSRLHHENIVRYYNAWIERHERPAVPGTPPPDYIPQAQNSSAT
                     GGKASGDTEELGSVEAAAPPPILSSSVEWSTSAERSNSARFPVTGQDSSSDEEDEDER
                     DGVFSQSFLPASDSDSDIIFDNEDENSKSQNQDEDCNEKDSRHEIEPSVTTEAVHYLY
                     IQMEYCEKSTLRDTIDQGLFRDTSRLWRLFREILDGLAYIHEKGMIHRDLKPVNIFLD
                     SDDHVKIGDFGLATDHLAFNAEGKQDDQAGDHVIKSDPSGHLTGMVGTALYVSPEVQG
                     STKSAYNQKVDLFSLGIILFEMSYHPMVTASERIFVLNQLRDPTSPKFPDDFEDGEHT
                     KQKSVISWLLNHDPAKRPTAMELLKSELLPPPQMEESELHEVLHHTLANTDGKAYRTM
                     MSQLFCQHSSPAIDYTYDSDILKGNFLIRTAKIQQLVCETIVRVFKRHGAVQLCTPLL
                     LPRNRQIYEHNEAALFMDHSGMLVMLPFDLRVPFARYVARNNILNLKRYCIERVFRPR
                     KLDRFHPKELLECAFDIVTSTANSSLPTAETIYTIYEVIQEFPALQERNYSIYLNHTM
                     LLKAILLHCGIPEDKLSQVYVILYDAVTEKLTRREVEAKFCNLSLSSNSLCRLYKFIE
                     QKGDLQDLTPTINSLIKQKTGIAQLVKYSLKDLEEVVGLLKKLGVKLQVSINLGLVYK
                     VQQHNGIIFQFLAFSKRRQRVVPEILAAGGRYDLLIPKFRGPQALGPVPTAVGVSIAI
                     DKIFAAVLNMGEPVTVSSCDLLVVSAGQMSMSRAINLTQKLWTAGITAEIMYDWSQSQ
                     EELQEYCRHHEITYVALVSDKEGSHVKVKSFEKERQTEKRVLESDLVDHVMQKLRTKV
                     GDERNFRDASDNLAVQTLKGSFSNASGLFEIHGTTVVPTVSVISPEKLSASTRRRHEI
                     QVQTRLQTTLANLHQKSSEIEILAVDLPKETILQFLSLEWDADEQAFNTTVKQLLSRL
                     PKQRYLKLVCDEIYNIKVEKKVSVLFLYSYRDDYYRILF"
     exon            237..349
                     /gene="Eif2ak4"
                     /inference="alignment:Splign:2.1.0"
     exon            350..452
                     /gene="Eif2ak4"
                     /inference="alignment:Splign:2.1.0"
     exon            453..605
                     /gene="Eif2ak4"
                     /inference="alignment:Splign:2.1.0"
     exon            606..686
                     /gene="Eif2ak4"
                     /inference="alignment:Splign:2.1.0"
     exon            687..835
                     /gene="Eif2ak4"
                     /inference="alignment:Splign:2.1.0"
     exon            836..951
                     /gene="Eif2ak4"
                     /inference="alignment:Splign:2.1.0"
     exon            952..1109
                     /gene="Eif2ak4"
                     /inference="alignment:Splign:2.1.0"
     exon            1110..1645
                     /gene="Eif2ak4"
                     /inference="alignment:Splign:2.1.0"
     exon            1646..1752
                     /gene="Eif2ak4"
                     /inference="alignment:Splign:2.1.0"
     exon            1753..1910
                     /gene="Eif2ak4"
                     /inference="alignment:Splign:2.1.0"
     exon            1911..2338
                     /gene="Eif2ak4"
                     /inference="alignment:Splign:2.1.0"
     exon            2339..2408
                     /gene="Eif2ak4"
                     /inference="alignment:Splign:2.1.0"
     exon            2409..2492
                     /gene="Eif2ak4"
                     /inference="alignment:Splign:2.1.0"
     exon            2493..2615
                     /gene="Eif2ak4"
                     /inference="alignment:Splign:2.1.0"
     exon            2616..2720
                     /gene="Eif2ak4"
                     /inference="alignment:Splign:2.1.0"
     exon            2721..2778
                     /gene="Eif2ak4"
                     /inference="alignment:Splign:2.1.0"
     exon            2779..2858
                     /gene="Eif2ak4"
                     /inference="alignment:Splign:2.1.0"
     exon            2859..2960
                     /gene="Eif2ak4"
                     /inference="alignment:Splign:2.1.0"
     exon            2961..3014
                     /gene="Eif2ak4"
                     /inference="alignment:Splign:2.1.0"
     exon            3015..3251
                     /gene="Eif2ak4"
                     /inference="alignment:Splign:2.1.0"
     exon            3252..3327
                     /gene="Eif2ak4"
                     /inference="alignment:Splign:2.1.0"
     exon            3328..3449
                     /gene="Eif2ak4"
                     /inference="alignment:Splign:2.1.0"
     exon            3450..3499
                     /gene="Eif2ak4"
                     /inference="alignment:Splign:2.1.0"
     exon            3500..3668
                     /gene="Eif2ak4"
                     /inference="alignment:Splign:2.1.0"
     exon            3669..3785
                     /gene="Eif2ak4"
                     /inference="alignment:Splign:2.1.0"
     exon            3786..3851
                     /gene="Eif2ak4"
                     /inference="alignment:Splign:2.1.0"
     exon            3852..4022
                     /gene="Eif2ak4"
                     /inference="alignment:Splign:2.1.0"
     exon            4023..4157
                     /gene="Eif2ak4"
                     /inference="alignment:Splign:2.1.0"
     exon            4158..4265
                     /gene="Eif2ak4"
                     /inference="alignment:Splign:2.1.0"
     exon            4266..4394
                     /gene="Eif2ak4"
                     /inference="alignment:Splign:2.1.0"
     exon            4395..4481
                     /gene="Eif2ak4"
                     /inference="alignment:Splign:2.1.0"
     exon            4482..4594
                     /gene="Eif2ak4"
                     /inference="alignment:Splign:2.1.0"
     exon            4595..4653
                     /gene="Eif2ak4"
                     /inference="alignment:Splign:2.1.0"
     exon            4654..4751
                     /gene="Eif2ak4"
                     /inference="alignment:Splign:2.1.0"
     exon            4752..4820
                     /gene="Eif2ak4"
                     /inference="alignment:Splign:2.1.0"
     exon            4821..4865
                     /gene="Eif2ak4"
                     /inference="alignment:Splign:2.1.0"
     exon            4866..4984
                     /gene="Eif2ak4"
                     /inference="alignment:Splign:2.1.0"
     exon            4985..5225
                     /gene="Eif2ak4"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 ccccagctcg gtcaaggcgc gggccggagc tccaccgccc tccggagaat caccgctacc
       61 tcgcctaggc cgctgtgcct gcccgggccg ccatggctgg gggccgcggg gccgcaggac
      121 gcggcccggc tgagccgcag gagagctatt cacagagaca ggaccacgag ctgcaggccc
      181 tggaggcgat ttacggctcg gacttccagg atctgcggcc ggatgcgcgc ggacgggtga
      241 gagagcctcc tgaaatcaat ttagttctgt accctcaggg cctggctggt gaggaagtgt
      301 acgtgcaggt ggagctgagg gtcaaatgcc caccgacata cccggatgta gttcccgaaa
      361 tagagttaaa aaacactaaa ggtctgtcga atgagagtgt taatttgtta aagtcacacc
      421 tggaagaact ggccaaaaag cagtgtggag aggtgatgat attcgaactg gcacaccacg
      481 tgcagtcgtt tctcagcgag cataacaagc cccctccaaa gtctttccat gaagaaatgc
      541 tggaaaggca ggctcaggag aagcagcaga ggttgctgga ggccaggcag aaagaggagc
      601 aggagcaacg tgaaatcctc cacgagattc agaaaaggaa agaggagatc aaggaagaga
      661 agaaaaggaa ggaaatggct aagcaggaac gtttggaaat tactagtttg acaaaccagg
      721 atcatgcctc taagagagac ccagcaggac acagggcagc tgccattctc catggaggct
      781 ctcctgactt tgtaggaaat ggtaaagccc gggcacactc ctcaggaagg tccaggcgag
      841 aacgtcagta ttctgtctgt agtggtgaag cctctcctgg ctcgtgtgac atcctctact
      901 tctgtgtggg tagcgctgat cagctcatgg tgcacaaagg gaaatgtgtt ggcagcgatg
      961 agcagctggg gaaggtggta tacaatgcat tggaaacagc cacgggcagc tttgtcttgt
     1021 tgtatgagtg ggtccttcag tggcagaaaa agatgggccc ttgccttacc agtcaagaga
     1081 aagagaagat cgataaatgt aaaaagcaga ttcaaggagc agaaacagaa ttcagctccc
     1141 tagtgaaact gagccatcca aacattgtcc gctactttgc gatgaactcc agagaggaga
     1201 aggattccat tgtggtcgac attctggcag aacacatcag tgggatctct ctcgctgcac
     1261 acctgagcca ctcaggccct gtgcccatgc accagctgcg caaatacaca gcgcagctcc
     1321 tggcgggcct ggactacctg caccgcaact cggtggtgca caaggtgctg agcaccgcca
     1381 gcgtcttggt ggatgctgag ggcaccgtca agataacaga ctacagcatc tctaagcgcc
     1441 tggcagacat ttgcaaggag gatgtttttg agcaaactcg agttcgcttt agtgacagtg
     1501 ctctgcctta taaaaccggg aagaaagggg atgtgtggcg tcttggcctc ctgctgttgt
     1561 ctcttagcca aggtcaggag tgtgaggagt atcctgtgac catccccagt gatctgccag
     1621 ctgacttcca ggacttcctg aagaagtgtg tttgcctgga cgacaaggaa aggtggagcc
     1681 ctcaacagtt gctgaaacac agctttataa accctcagcc aaaaatgcct ttggtggagc
     1741 agagtcctga agattctggg ggacaagatt acatcgagac catcatcccc agcaaccagc
     1801 tgcccagcgc tgcgttcttc actgagacac agagacagtt ctctcgatac ttcattgagt
     1861 ttgaagaact acagcttctg gggaaaggag cttttggagc tgtcatcaag gtgcaaaaca
     1921 agcttgatgg ctgttgctat gctgtgaagc gcatccctat caaccctgca agcaggcagt
     1981 tccgcaggat caagggcgag gtgacgctgc tatcgcgcct gcaccatgag aacattgtac
     2041 gctactacaa cgcctggatc gaacggcatg agcgcccagc ggtgccaggg acaccacccc
     2101 cagactacat accccaggcc cagaacagct cagccaccgg cgggaaagca tcaggcgaca
     2161 ctgaggaact gggcagcgtg gaggctgcag caccaccgcc catcctcagc agctcagtgg
     2221 agtggagcac atctgcagag cgctctaaca gcgcccgttt cccagtcaca ggccaggact
     2281 ccagcagcga tgaggaagat gaggacgagc gcgatggtgt cttctcacag tcctttctac
     2341 ctgcttcaga ttctgacagt gacatcatct ttgacaatga agatgaaaac agtaaaagtc
     2401 agaatcagga tgaagactgc aatgaaaagg acagtcgcca tgagattgag ccatcagtga
     2461 cgaccgaggc tgtgcactac ctgtacatcc agatggagta ctgcgagaag agcacactgc
     2521 gagacaccat tgaccagggg ctgttccgag acaccagccg gctctggagg cttttccgag
     2581 agattctgga tggattagct tatatccatg agaaaggaat gattcaccgg gatttgaagc
     2641 ctgtcaacat ttttttggat tctgatgacc atgtgaaaat aggtgatttt ggtttggcaa
     2701 cagaccatct ggccttcaat gctgaaggta aacaggacga ccaagcaggt gatcatgtga
     2761 ttaagtcaga cccttcaggc catttgactg gcatggttgg caccgctctg tatgtaagcc
     2821 ctgaggtcca aggaagtacc aagtctgcgt acaaccagaa agtggatctc ttcagcctgg
     2881 gaattatcct ctttgagatg tcgtatcacc cgatggtgac tgcctcagaa agaattttcg
     2941 ttctcaacca acttcgagat cccacgtcac ccaagttccc agacgacttt gaggacggag
     3001 agcatacaaa gcagaaatct gtcatctcct ggctgttaaa ccatgatcca gccaaacggc
     3061 ccacagccat ggagctgctc aagagtgagc tgctcccccc accacagatg gaggagtcgg
     3121 agctgcatga agtgctgcac cacacgctgg ccaacacgga tgggaaggcc taccgcacca
     3181 tgatgagcca gctcttctgc cagcacagct cccccgccat agactacacc tacgacagcg
     3241 acatcctgaa gggcaacttc ttgattcgca cagccaagat ccagcagctt gtgtgtgaaa
     3301 ccatcgtccg cgtcttcaaa aggcatggag ctgtccagct gtgcaccccg ctgctgcttc
     3361 cccgaaacag gcaaatatat gagcacaacg aagccgctct gttcatggat cacagtggca
     3421 tgctggtgat gcttcctttc gacctgcggg tcccttttgc aagatatgtg gcaagaaata
     3481 acatactgaa tttaaagcga tactgcatag agcgggtgtt cagacctagg aagttagacc
     3541 gatttcatcc caaagaactc ctggaatgtg catttgacat cgtcacttct accgccaaca
     3601 gctccctgcc cactgctgaa accatctaca ccatctatga agtcatccag gagtttcctg
     3661 cacttcagga aagaaattac agcatttact tgaaccatac aatgctccta aaggcaatac
     3721 tcctacactg tgggatccca gaggataaac tcagtcaagt ctacgttatt ctgtatgatg
     3781 ctgtgacaga gaagctgact aggagggaag tagaagctaa gttctgtaac ctgtccttgt
     3841 catctaatag tctgtgtcga ctctataagt tcatcgagca gaagggggat ttgcaagacc
     3901 taacgccgac cataaactca ctaataaaac agaaaacagg cattgctcag ctggtgaagt
     3961 atagcttaaa agacctagag gaagttgtcg ggctgctgaa gaaacttggc gtgaagttgc
     4021 aggtctccat caacctgggc ttggtttaca aggtgcagca gcacaatggc atcatcttcc
     4081 agttcctggc attcagcaaa cgcaggcaga gggttgtgcc tgagatcctt gcagctggtg
     4141 gcagatatga cctgctgatt cccaagttca gaggcccaca ggctctgggg ccagtgccca
     4201 ctgctgtcgg tgtcagcata gccatagaca agatattcgc cgctgtcctc aacatgggag
     4261 agcctgttac agtgagctcc tgtgacctcc tggttgtcag tgctggccag atgtccatgt
     4321 ccagggccat caacctaacc cagaaactct ggacggccgg catcactgca gagatcatgt
     4381 atgactggtc acagtcccag gaagagttac aagaatactg cagacatcac gaaatcacct
     4441 atgtggccct ggtctctgat aaagaaggaa gccatgtcaa ggtcaagtcc tttgagaagg
     4501 agaggcaaac agaaaaacgt gtgttggaat cagatcttgt ggaccatgtt atgcagaaac
     4561 taaggaccaa agttggtgac gaaaggaatt tcagagacgc ttctgataat cttgccgtac
     4621 aaactctgaa ggggtcgttt tctaatgctt caggtttgtt tgaaatccat ggaacaacag
     4681 tagtccccac tgtgagtgtt atatcaccag aaaaactgtc ggccagcacc aggaggcgac
     4741 atgagattca ggtgcaaacc cgacttcaga ctacccttgc caatttacat cagaaaagca
     4801 gtgaaattga aattttggct gtggatctac ccaaggagac catcttacag tttctatcgc
     4861 tagagtggga cgctgatgaa caggcattta acacaaccgt gaaacagctg ctgtcacgcc
     4921 tgccaaagca aagatacctc aaactcgtct gcgacgaaat ctataacatc aaagttgaga
     4981 agaaggtgtc ggtgttgttc ctgtacagct acagagatga ctactacaga atcctattct
     5041 gaccctaaag aactgctgct gtattgttca gatggacagg gacttgtact ggaataatgg
     5101 aatgttgtgc attcaatcca ttttaacttt aaattctaat aagctgtcct acaaagctga
     5161 gctttgtcgg tttttcatgt gttataaatt cacctttttg atataataaa tgttttcata
     5221 tatat
//
