LOCUS       NM_031599               4509 bp    mRNA    linear   ROD 08-DEC-2024
DEFINITION  Rattus norvegicus eukaryotic translation initiation factor 2 alpha
            kinase 3 (Eif2ak3), mRNA.
ACCESSION   NM_031599
VERSION     NM_031599.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 4509)
  AUTHORS   Yuan,Z., Xiao-Wei,L., Juan,W., Xiu-Juan,L., Nian-Yun,Z. and Lei,S.
  TITLE     HIIT and MICT attenuate high-fat diet-induced hepatic lipid
            accumulation and ER stress via the PERK-ATF4-CHOP signaling pathway
  JOURNAL   J Physiol Biochem 78 (3), 641-652 (2022)
   PUBMED   35315506
  REMARK    GeneRIF: HIIT and MICT attenuate high-fat diet-induced hepatic
            lipid accumulation and ER stress via the PERK-ATF4-CHOP signaling
            pathway.
REFERENCE   2  (bases 1 to 4509)
  AUTHORS   Liu,H., Li,J., Jiang,C., Yuan,T. and Ma,H.
  TITLE     Cellular communication network factor 1 (CCN1) knockdown exerts a
            protective effect for hepatic ischemia/reperfusion injury by
            deactivating the MEK/ERK pathway
  JOURNAL   Clin Res Hepatol Gastroenterol 45 (5), 101737 (2021)
   PUBMED   34144219
REFERENCE   3  (bases 1 to 4509)
  AUTHORS   Nouri,H., Sheikholeslami-Vatani,D. and Moloudi,M.R.
  TITLE     Changes in UPR-PERK pathway and muscle hypertrophy following
            resistance training and creatine supplementation in rats
  JOURNAL   J Physiol Biochem 77 (2), 331-339 (2021)
   PUBMED   33635524
  REMARK    GeneRIF: Changes in UPR-PERK pathway and muscle hypertrophy
            following resistance training and creatine supplementation in rats.
REFERENCE   4  (bases 1 to 4509)
  AUTHORS   Zhang,R., Jiang,M., Zhang,J., Qiu,Y., Li,D., Li,S., Liu,J., Liu,C.,
            Fang,Z. and Cao,F.
  TITLE     Regulation of the cerebrovascular smooth muscle cell phenotype by
            mitochondrial oxidative injury and endoplasmic reticulum stress in
            simulated microgravity rats via the PERK-eIF2alpha-ATF4-CHOP
            pathway
  JOURNAL   Biochim Biophys Acta Mol Basis Dis 1866 (8), 165799 (2020)
   PUBMED   32304741
  REMARK    GeneRIF: Regulation of the cerebrovascular smooth muscle cell
            phenotype by mitochondrial oxidative injury and endoplasmic
            reticulum stress in simulated microgravity rats via the
            PERK-eIF2alpha-ATF4-CHOP pathway.
REFERENCE   5  (bases 1 to 4509)
  AUTHORS   Manaud,G., Nossent,E.J., Lambert,M., Ghigna,M.R., Boet,A.,
            Vinhas,M.C., Ranchoux,B., Dumas,S.J., Courboulin,A., Girerd,B.,
            Soubrier,F., Bignard,J., Claude,O., Lecerf,F., Hautefort,A.,
            Florio,M., Sun,B., Nadaud,S., Verleden,S.E., Remy,S., Anegon,I.,
            Bogaard,H.J., Mercier,O., Fadel,E., Simonneau,G., Vonk
            Noordegraaf,A., Grunberg,K., Humbert,M., Montani,D., Dorfmuller,P.,
            Antigny,F. and Perros,F.
  TITLE     Comparison of Human and Experimental Pulmonary Veno-Occlusive
            Disease
  JOURNAL   Am J Respir Cell Mol Biol 63 (1), 118-131 (2020)
   PUBMED   32209028
REFERENCE   6  (bases 1 to 4509)
  AUTHORS   Bertolotti,A., Zhang,Y., Hendershot,L.M., Harding,H.P. and Ron,D.
  TITLE     Dynamic interaction of BiP and ER stress transducers in the
            unfolded-protein response
  JOURNAL   Nat Cell Biol 2 (6), 326-332 (2000)
   PUBMED   10854322
REFERENCE   7  (bases 1 to 4509)
  AUTHORS   Sood,R., Porter,A.C., Ma,K., Quilliam,L.A. and Wek,R.C.
  TITLE     Pancreatic eukaryotic initiation factor-2alpha kinase (PEK)
            homologues in humans, Drosophila melanogaster and Caenorhabditis
            elegans that mediate translational control in response to
            endoplasmic reticulum stress
  JOURNAL   Biochem J 346 Pt 2 (Pt 2), 281-293 (2000)
   PUBMED   10677345
REFERENCE   8  (bases 1 to 4509)
  AUTHORS   Shi,Y., An,J., Liang,J., Hayes,S.E., Sandusky,G.E., Stramm,L.E. and
            Yang,N.N.
  TITLE     Characterization of a mutant pancreatic eIF-2alpha kinase, PEK, and
            co-localization with somatostatin in islet delta cells
  JOURNAL   J Biol Chem 274 (9), 5723-5730 (1999)
   PUBMED   10026192
REFERENCE   9  (bases 1 to 4509)
  AUTHORS   Harding,H.P., Zhang,Y. and Ron,D.
  TITLE     Protein translation and folding are coupled by an
            endoplasmic-reticulum-resident kinase
  JOURNAL   Nature 397 (6716), 271-274 (1999)
   PUBMED   9930704
  REMARK    Erratum:[Nature 1999 Mar 4;398(6722):90]
REFERENCE   10 (bases 1 to 4509)
  AUTHORS   Shi,Y., Vattem,K.M., Sood,R., An,J., Liang,J., Stramm,L. and
            Wek,R.C.
  TITLE     Identification and characterization of pancreatic eukaryotic
            initiation factor 2 alpha-subunit kinase, PEK, involved in
            translational control
  JOURNAL   Mol Cell Biol 18 (12), 7499-7509 (1998)
   PUBMED   9819435
COMMENT     PROVISIONAL REFSEQ: This record has not yet been subject to final
            NCBI review. The reference sequence was derived from AF096835.1.
            
            On Aug 31, 2012 this sequence version replaced NM_031599.1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AF096835.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMD00132285, SAMD00132291
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-4509              AF096835.1         2-4510
FEATURES             Location/Qualifiers
     source          1..4509
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /db_xref="taxon:10116"
                     /chromosome="4"
                     /map="4q32"
     gene            1..4509
                     /gene="Eif2ak3"
                     /gene_synonym="PEK"
                     /note="eukaryotic translation initiation factor 2 alpha
                     kinase 3"
                     /db_xref="GeneID:29702"
                     /db_xref="RGD:70884"
     exon            1..504
                     /gene="Eif2ak3"
                     /gene_synonym="PEK"
                     /inference="alignment:Splign:2.1.0"
     CDS             212..3538
                     /gene="Eif2ak3"
                     /gene_synonym="PEK"
                     /EC_number="2.7.11.1"
                     /EC_number="2.7.10.2"
                     /note="pancreatic eIF2-alpha kinase; PRKR-like endoplasmic
                     reticulum kinase; protein tyrosine kinase EIF2AK3"
                     /codon_start=1
                     /product="eukaryotic translation initiation factor 2-alpha
                     kinase 3 precursor"
                     /protein_id="NP_113787.1"
                     /db_xref="GeneID:29702"
                     /db_xref="RGD:70884"
                     /translation="MERATQPRPRALLLLFLLLGCAAGISAVARARSLLAPTSDTAFG
                     LGAAAAPTSAARVPAVATAEVTVEDAEALPAASGEQESRATESDDDVELRPRGRSLVI
                     ISTLDGRIAALDAENHGKKQWDLDVGSGSLVSSSLSKPEVFGNKMIIPSLDGDLFQWD
                     RDRESMEAVPFTVESLLESSYKFGDDVVLVGGKSLTTYGLSAYSGKLRYICSALGCRR
                     WDSDEMEEEEDILLLQRTQKTVRAVGPRSGSEKWNFSVGHFELRYIPDMETRAGFIES
                     TFKLGGNKEDSKIISDVEEQDVDTVIKVSVADWKVMAFSKKGGRLEWEYQFCTPIASA
                     WLVRDGKVIPISLFDDTSYTANEEVLEDEEDIVEAARGATENSVYLGMYRGQLYLQSS
                     VRVSEKFPTRPKALESVNGESAIIPLPTIKWKPLIHSPSRTPVLVGSDEFDKCLSNDK
                     YSHEEYSNGALSILQYPYDNGYYLPYYKRERNKRSTQITVRFLDSPHYSKNIRKKDPI
                     LLLHWWKEIFGTILLCIVATTFIVRRLFHPQPHRQRKESETQCQTESKYDSVSADNSD
                     NSWNDIKHSGYVSRYLTDFEPIQCMGRGGFGVVFEAKNKVDDCNYAIKRIRLPNRELA
                     REKVMREVKALAKLEHPGIVRYFNAWLETPPEKWQEEMDEIWLKDESTDWPLSSPSPM
                     DAPSVKIRQMDPFSTKEQIEVIAPSPERSRSFSVGISCGRTSSSESQFSPLEFSGTDC
                     GDNSDSEDAAHNLQDSCLTDCDMEDGTVDGDDEGHSFELCPSEASPYTRSREGTSSSI
                     VFEDSGCDNASSKEDPRMNRLHNGHHYVNKLTEFKHSSSRSSSEATLSTSPTRPTTLS
                     LDFTRNTVDRLQPSSPKVYLYIQMQLCRKENLKDWMNRRCSMEDREHRVCLHIFLQIA
                     EAVQFLHSKGLMHRDLKPSNIFFTMDDVVKVGDFGLVTAMDQDEEEQTVLTPMPAYAT
                     HTGQVGTKLYMSPEQIHGNNYSHKVDIFSLGLILFELLYPFSTQMERVRTLTDVRNLK
                     FPPLFTQKYPQEHMMVQDMLSPSPMERPEATDIIENAVFENLEFPGKTVLRQRSRSLS
                     SSGTKHSRQPSSTFSPLPGN"
     exon            505..634
                     /gene="Eif2ak3"
                     /gene_synonym="PEK"
                     /inference="alignment:Splign:2.1.0"
     exon            635..829
                     /gene="Eif2ak3"
                     /gene_synonym="PEK"
                     /inference="alignment:Splign:2.1.0"
     exon            830..963
                     /gene="Eif2ak3"
                     /gene_synonym="PEK"
                     /inference="alignment:Splign:2.1.0"
     exon            964..1189
                     /gene="Eif2ak3"
                     /gene_synonym="PEK"
                     /inference="alignment:Splign:2.1.0"
     exon            1190..1352
                     /gene="Eif2ak3"
                     /gene_synonym="PEK"
                     /inference="alignment:Splign:2.1.0"
     exon            1353..1493
                     /gene="Eif2ak3"
                     /gene_synonym="PEK"
                     /inference="alignment:Splign:2.1.0"
     exon            1494..1616
                     /gene="Eif2ak3"
                     /gene_synonym="PEK"
                     /inference="alignment:Splign:2.1.0"
     exon            1617..1837
                     /gene="Eif2ak3"
                     /gene_synonym="PEK"
                     /inference="alignment:Splign:2.1.0"
     exon            1838..1950
                     /gene="Eif2ak3"
                     /gene_synonym="PEK"
                     /inference="alignment:Splign:2.1.0"
     exon            1951..2073
                     /gene="Eif2ak3"
                     /gene_synonym="PEK"
                     /inference="alignment:Splign:2.1.0"
     exon            2074..2223
                     /gene="Eif2ak3"
                     /gene_synonym="PEK"
                     /inference="alignment:Splign:2.1.0"
     exon            2224..3004
                     /gene="Eif2ak3"
                     /gene_synonym="PEK"
                     /inference="alignment:Splign:2.1.0"
     exon            3005..3172
                     /gene="Eif2ak3"
                     /gene_synonym="PEK"
                     /inference="alignment:Splign:2.1.0"
     exon            3173..3274
                     /gene="Eif2ak3"
                     /gene_synonym="PEK"
                     /inference="alignment:Splign:2.1.0"
     exon            3275..3337
                     /gene="Eif2ak3"
                     /gene_synonym="PEK"
                     /inference="alignment:Splign:2.1.0"
     exon            3338..4492
                     /gene="Eif2ak3"
                     /gene_synonym="PEK"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 cggaaccctc gctcaatggg cgatgtctgt acaaggctgt cactcaggtg gcagtggctg
       61 agacgtggcc gggcagctct gctgctgcgg cgtgaagtcg agaggcgtcg gggtccgtgg
      121 tgcgcgcttt cggtgctcca aggctcctag cggcgagacg ggccggcgcc gaccgcaggg
      181 tctccatgcc cgctcgtggg gcgggccgct gatggagcgc gccacccagc cccgaccgcg
      241 cgcgctgctg ctgctgttcc tgctgctggg ctgcgcggcg gggatctcag cggtcgcgcg
      301 cgcccgcagc ttgcttgctc ccacatcgga tacggcattt ggcttggggg cagcagccgc
      361 ccctacttca gccgcgcggg tgcctgcggt ggctacggcc gaagtgaccg tggaagacgc
      421 cgaggcattg cccgctgcct ccggcgaaca ggagtcacgc gcgaccgagt ccgatgacga
      481 cgtggaactg cggcctcgcg gcaggtcctt agtaatcatc agcactttag atggacgaat
      541 tgccgcactg gatgccgaga atcatgggaa aaagcagtgg gatttggacg tgggatctgg
      601 ttccttggtt tcatctagcc ttagcaagcc agaggtgttt gggaacaaga tgatcattcc
      661 ttccctggac ggagacctct tccagtggga ccgagaccga gagagcatgg aggccgtccc
      721 tttcactgtg gagtcccttc tcgaatcttc atacaagttt ggagatgatg ttgttctggt
      781 tggaggaaag tctctgacca catacgggct cagtgcatat agtggaaagc tgaggtatat
      841 ctgttctgcc ttgggatgtc gccgatggga tagtgatgaa atggaagaag aggaagacat
      901 cttgcttctg caacgcacgc agaagactgt gcgagctgtc gggcctcgaa gcggcagtga
      961 gaagtggaat ttcagtgttg gccactttga acttcggtat attccagaca tggaaactag
     1021 agccggattc attgaaagca cctttaaact gggtggaaac aaagaagact ctaaaatcat
     1081 ttcagatgtg gaagaacaag atgtggacac agtgataaaa gtttccgttg ctgattggaa
     1141 ggtcatggcg tttagtaaga agggaggccg cctagaatgg gagtaccagt tttgtactcc
     1201 cattgcgtct gcctggctgg tgagggatgg taaagtcatc cccatcagtc tgtttgatga
     1261 cacaagttac acagccaatg aggaagtttt ggaagatgaa gaagacattg tagaggctgc
     1321 tcggggagcc acagagaaca gcgtgtactt agggatgtac agaggccagc tgtacctgca
     1381 atcatccgtc agggtctcgg aaaagttccc tacaaggccg aaggccttgg agtctgtaaa
     1441 tggagaaagt gccattattc ctctgccgac gatcaaatgg aagcccttaa tccattctcc
     1501 gtctaggact cctgtcttgg ttgggtctga tgaatttgac aagtgtctaa gtaatgataa
     1561 atattcccat gaagaataca gtaatggtgc gctttcaatc ctacagtatc catatgacaa
     1621 cggttactat ctgccttatt acaagagaga aaggaataag cggagcacgc agatcacggt
     1681 caggttcctg gacagcccgc actacagcaa gaacatccgc aagaaggacc ccatcctcct
     1741 cctgcactgg tggaaggaaa tatttggaac gatcttgctt tgcattgtgg ccacgacctt
     1801 tatcgtgcgc aggcttttcc atcctcagcc ccacaggcaa cggaaggagt ctgaaactca
     1861 gtgccaaact gaaagtaaat acgactctgt gagtgctgat aacagcgaca acagctggaa
     1921 cgacattaaa cactcaggat acgtgtcccg atacctaaca gattttgagc caattcagtg
     1981 catgggtcga ggcggctttg gcgttgtctt tgaagctaag aacaaagtag atgactgcaa
     2041 ttacgccatc aagaggatcc gtctcccaaa cagggagctg gcacgggaga aggtaatgcg
     2101 ggaagtcaaa gccttggcta agctggagca cccaggcatc gtgaggtatt tcaatgcgtg
     2161 gctggaaaca ccaccagaga agtggcaaga ggagatggat gaaatctggc tgaaggacga
     2221 aagcacagac tggccactca gctcccctag cccaatggat gccccatctg ttaagatccg
     2281 gcagatggat cctttctcta caaaagaaca gattgaagtc atagctcctt ctcctgaaag
     2341 aagtcggtct ttctcagtgg gcatttcctg tggccggaca agctcatctg agagccagtt
     2401 ctctcccctg gagttctcag ggacagactg tggagacaac agtgactcag aggacgcagc
     2461 ccacaacctc caggacagct gcctgacaga ttgcgacatg gaagatggta cagtggacgg
     2521 cgatgatgag ggacactcct ttgaactttg tccttctgaa gcttctccct ataccaggtc
     2581 aagggaggga acgtcctctt ccatagtgtt tgaagactct ggctgtgata atgcgtccag
     2641 taaggaggac cccagaatga atcggctgca taatggccac cattacgtta ataagctaac
     2701 tgagttcaag cactccagca gcaggtcttc ttccgaagcc accttgtcta cctctcctac
     2761 caggccaacc accctaagtt tagatttcac caggaacacg gtggaccggc tccagcccag
     2821 ctcccccaag gtgtacctgt acatccagat gcagctgtgc aggaaggaga accttaaaga
     2881 ctggatgaac cggcgctgca gcatggagga cagggagcac agagtgtgcc tgcacatctt
     2941 tctgcagatc gcagaggcag tgcagtttct gcacagcaag ggactcatgc acagggacct
     3001 caagccttcc aacatattct tcacaatgga tgatgtggtc aaagttgggg actttgggct
     3061 agtgactgca atggaccagg atgaagaaga gcagacagta ctgactccaa tgccagccta
     3121 tgctacacac acgggacaag tcgggaccaa gctgtacatg agcccagaac agattcatgg
     3181 aaacaactac tcccataaag tggacatctt ctctttaggc ttgattctgt ttgaactcct
     3241 ttacccattc agcactcaga tggaacgagt taggacttta actgatgtaa gaaatctcaa
     3301 gtttccacca ctgttcactc agaaatatcc ccaagagcat atgatggttc aagacatgct
     3361 ttctccatcc cccatggagc ggcctgaagc cacagacatc attgaaaatg ctgtgtttga
     3421 gaacttggag tttcctggga aaacggttct gagacagcgg tcccgctcct tgagttcatc
     3481 cggaactaaa cattccagac agcccagcag cacattcagc ccactgcctg gcaactagtc
     3541 tccagctgtg cctgcggccc tcagaggtgg cacaggacgg gcaccttgag gaatgtggct
     3601 ctccgtggtg gcggactcat tttatgcttt gatcaacttg acttaggacc aatttttcta
     3661 agtcagactg gattatgggc ctaacccagt ttgatcttaa ctaaacttca aggaaagggc
     3721 tatataaatg acaagtggat ttgtttcttg ttggtgtccc aaaactagct ggtcaagcta
     3781 cagaaccttc acttttcacc aggaggcaga agacagcctg aaaccatctg aactggaaat
     3841 gactttaaag cacaaaggtg tgaaagaccc tctcacatgg gaactacatg ttctagaaac
     3901 gtgctttcta gaaatacaag ggtgattatg gaagcgactg ttataaagct gactccattt
     3961 ttgtccctgg tgagcatgtg acccatctgc actattttgg aaggcgcagc acaggctggg
     4021 tggccattta tgtcagtagt gccatagtct gcagcagtga ataacttcac tttttaggtg
     4081 gtctagggag cacggtgagc tttttgtact tttttatctc cagtaatggg aaaatgaagc
     4141 tttttaggta ttggtcaaaa gatctgattg gagggtttaa aaagaagtgt agtaagaaat
     4201 gggtcaccta ttacacctaa cttattccat tatggagttt ttgtcctagt agaagtctac
     4261 cttatgtgta atactacaag tcagcatatc ccccaaactg attataggta agtttaatca
     4321 tctcaacttg ctaacatgtt ctcatttttc ctgtaaatat ctttattttt tatttataaa
     4381 aattctgaaa tcaatccatt tgggttggtg gtgtacagga tgcacataag tgtgttaact
     4441 attatgactt ctttcaagtc taaatgattt aataaaacgt ttttaaatta taaaaaaaaa
     4501 aaaaaaaaa
//
