LOCUS       NM_001415868            5017 bp    mRNA    linear   ROD 31-MAR-2024
DEFINITION  Rattus norvegicus euchromatic histone lysine methyltransferase 1
            (Ehmt1), transcript variant 2, mRNA.
ACCESSION   NM_001415868 XM_006233618
VERSION     NM_001415868.1
KEYWORDS    RefSeq.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 5017)
  AUTHORS   Wang,Y., Zhang,Y., Qu,Y., Li,S., Xi,W., Liu,B. and Ye,L.
  TITLE     eIF4A3-mediated circEHMT1 regulation in retinal microvascular
            endothelial dysfunction in diabetic retinopathy
  JOURNAL   Microvasc Res 151, 104612 (2024)
   PUBMED   37839527
  REMARK    GeneRIF: eIF4A3-mediated circEHMT1 regulation in retinal
            microvascular endothelial dysfunction in diabetic retinopathy.
REFERENCE   2  (bases 1 to 5017)
  AUTHORS   Sharma,M., Dierkes,T. and Sajikumar,S.
  TITLE     Epigenetic regulation by G9a/GLP complex ameliorates amyloid-beta
            1-42 induced deficits in long-term plasticity and synaptic
            tagging/capture in hippocampal pyramidal neurons
  JOURNAL   Aging Cell 16 (5), 1062-1072 (2017)
   PUBMED   28665013
  REMARK    GeneRIF: Epigenetic regulation by G9a/GLP complex ameliorates
            amyloid-beta induced deficits in long-term plasticity and synaptic
            tagging/capture in hippocampal pyramidal neurons.
REFERENCE   3  (bases 1 to 5017)
  AUTHORS   Thienpont,B., Aronsen,J.M., Robinson,E.L., Okkenhaug,H., Loche,E.,
            Ferrini,A., Brien,P., Alkass,K., Tomasso,A., Agrawal,A.,
            Bergmann,O., Sjaastad,I., Reik,W. and Roderick,H.L.
  TITLE     The H3K9 dimethyltransferases EHMT1/2 protect against pathological
            cardiac hypertrophy
  JOURNAL   J Clin Invest 127 (1), 335-348 (2017)
   PUBMED   27893464
  REMARK    GeneRIF: miR-217-mediated, genetic, or pharmacological inactivation
            of EHMT1/2 was sufficient to promote pathological hypertrophy
REFERENCE   4  (bases 1 to 5017)
  AUTHORS   Zhong,X., Chen,X., Guan,X., Zhang,H., Ma,Y., Zhang,S., Wang,E.,
            Zhang,L. and Han,Y.
  TITLE     Overexpression of G9a and MCM7 in oesophageal squamous cell
            carcinoma is associated with poor prognosis
  JOURNAL   Histopathology 66 (2), 192-200 (2015)
   PUBMED   24805087
REFERENCE   5  (bases 1 to 5017)
  AUTHORS   Guan,X., Zhong,X., Men,W., Gong,S., Zhang,L. and Han,Y.
  TITLE     Analysis of EHMT1 expression and its correlations with clinical
            significance in esophageal squamous cell cancer
  JOURNAL   Mol Clin Oncol 2 (1), 76-80 (2014)
   PUBMED   24649311
REFERENCE   6  (bases 1 to 5017)
  AUTHORS   Tachibana,M., Matsumura,Y., Fukuda,M., Kimura,H. and Shinkai,Y.
  TITLE     G9a/GLP complexes independently mediate H3K9 and DNA methylation to
            silence transcription
  JOURNAL   EMBO J 27 (20), 2681-2690 (2008)
   PUBMED   18818694
REFERENCE   7  (bases 1 to 5017)
  AUTHORS   Wang,J., Scully,K., Zhu,X., Cai,L., Zhang,J., Prefontaine,G.G.,
            Krones,A., Ohgi,K.A., Zhu,P., Garcia-Bassets,I., Liu,F., Taylor,H.,
            Lozach,J., Jayes,F.L., Korach,K.S., Glass,C.K., Fu,X.D. and
            Rosenfeld,M.G.
  TITLE     Opposing LSD1 complexes function in developmental gene activation
            and repression programmes
  JOURNAL   Nature 446 (7138), 882-887 (2007)
   PUBMED   17392792
REFERENCE   8  (bases 1 to 5017)
  AUTHORS   Ueda,J., Tachibana,M., Ikura,T. and Shinkai,Y.
  TITLE     Zinc finger protein Wiz links G9a/GLP histone methyltransferases to
            the co-repressor molecule CtBP
  JOURNAL   J Biol Chem 281 (29), 20120-20128 (2006)
   PUBMED   16702210
REFERENCE   9  (bases 1 to 5017)
  AUTHORS   Tachibana,M., Ueda,J., Fukuda,M., Takeda,N., Ohta,T., Iwanari,H.,
            Sakihama,T., Kodama,T., Hamakubo,T. and Shinkai,Y.
  TITLE     Histone methyltransferases G9a and GLP form heteromeric complexes
            and are both crucial for methylation of euchromatin at H3-K9
  JOURNAL   Genes Dev 19 (7), 815-826 (2005)
   PUBMED   15774718
REFERENCE   10 (bases 1 to 5017)
  AUTHORS   Ogawa,H., Ishiguro,K., Gaubatz,S., Livingston,D.M. and Nakatani,Y.
  TITLE     A complex with chromatin modifiers that occupies E2F- and
            Myc-responsive genes in G0 cells
  JOURNAL   Science 296 (5570), 1132-1136 (2002)
   PUBMED   12004135
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            JAXUCZ010000003.1.
            
            On Jan 4, 2023 this sequence version replaced XM_006233618.4.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR26643298.14252.1,
                                           SRR26643294.12121.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA5760383,
                                           SAMEA5760386 [ECO:0006172]
            ##Evidence-Data-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-39                JAXUCZ010000003.1  28127088-28127126   c
            40-106              JAXUCZ010000003.1  28071220-28071286   c
            107-663             JAXUCZ010000003.1  28068312-28068868   c
            664-835             JAXUCZ010000003.1  28062140-28062311   c
            836-993             JAXUCZ010000003.1  28048642-28048799   c
            994-1182            JAXUCZ010000003.1  28048005-28048193   c
            1183-1260           JAXUCZ010000003.1  28043446-28043523   c
            1261-1381           JAXUCZ010000003.1  28041866-28041986   c
            1382-1519           JAXUCZ010000003.1  28036983-28037120   c
            1520-1665           JAXUCZ010000003.1  28032297-28032442   c
            1666-1809           JAXUCZ010000003.1  28024287-28024430   c
            1810-2036           JAXUCZ010000003.1  28023167-28023393   c
            2037-2204           JAXUCZ010000003.1  28021780-28021947   c
            2205-2287           JAXUCZ010000003.1  28020628-28020710   c
            2288-2394           JAXUCZ010000003.1  28019465-28019571   c
            2395-2517           JAXUCZ010000003.1  28010239-28010361   c
            2518-2619           JAXUCZ010000003.1  28002242-28002343   c
            2620-2724           JAXUCZ010000003.1  28000519-28000623   c
            2725-2879           JAXUCZ010000003.1  27992947-27993101   c
            2880-3047           JAXUCZ010000003.1  27992091-27992258   c
            3048-3192           JAXUCZ010000003.1  27991756-27991900   c
            3193-3270           JAXUCZ010000003.1  27991005-27991082   c
            3271-3386           JAXUCZ010000003.1  27990088-27990203   c
            3387-3473           JAXUCZ010000003.1  27989244-27989330   c
            3474-3552           JAXUCZ010000003.1  27988731-27988809   c
            3553-3728           JAXUCZ010000003.1  27980299-27980474   c
            3729-5017           JAXUCZ010000003.1  27978888-27980176   c
FEATURES             Location/Qualifiers
     source          1..5017
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="BN"
                     /db_xref="taxon:10116"
                     /chromosome="3"
                     /map="3p13"
     gene            1..5017
                     /gene="Ehmt1"
                     /note="euchromatic histone lysine methyltransferase 1"
                     /db_xref="GeneID:362078"
                     /db_xref="RGD:1307588"
     exon            1..39
                     /gene="Ehmt1"
                     /inference="alignment:Splign:2.1.0"
     CDS             19..3909
                     /gene="Ehmt1"
                     /EC_number="2.1.1.354"
                     /note="isoform 2 is encoded by transcript variant 2;
                     histone-lysine N-methyltransferase, H3 lysine-9 specific
                     5; euchromatic histone methyltransferase 1; histone-lysine
                     N-methyltransferase EHMT1; euchromatic histone-lysine
                     N-methyltransferase 1; histone-lysine N-methyltransferase
                     EHMT1-like"
                     /codon_start=1
                     /product="histone-lysine N-methyltransferase EHMT1 isoform
                     2"
                     /protein_id="NP_001402797.1"
                     /db_xref="GeneID:362078"
                     /db_xref="RGD:1307588"
                     /translation="MAAADAEQAVLAKQETKQDCCMKTELLREDTLMAADEGSTEKQA
                     GETPMAADGETNGSCEKSGDISHPNAPKHTQENTRASPQEGTNRVSRVAENGVSERDT
                     EVGKQNHVTADDFMQTSVIGSNGYFLNKPALQGQPLRTPNTLNSSLPGHAAKTLPGGA
                     SKCRTPSALPQTPTTAPTVPGEGSADTEDRKPTASGTDVRVHRARKTMPKSILGLHAA
                     SKDHREVQDHKEPKEDINRNISECGRQQLLPTFPALHQSLPQNQCYMATTKSQTACLP
                     FVLAAAVSRKKKRRMGTYSLVPKKKTKVLKQRTVIEMFKSITHSTVGAKGEKVLDDSA
                     LHVNGESLEMDSEEEDSEELEDEEDRGAEQAAAFPTEDSRTSKESMSETDRAAKMDGD
                     SEEEQESPDTGEDEDGGDESDLSSESSIKKKFLKRRGKTDSPWIKPARKRRRRSRKKP
                     SSMLGSEACKSSPGSMEQAALGDSAGYMEVSLDSLDLRVRGILSSQTENEGLANGPDV
                     LETDGLHEVPLCSCRMETPKSREISTLANNQCMATESVDHELGRCTNSVVKYELMRPS
                     NKAPLLVLCEDHRGRMVKHQCCPGCGYFCTAGNFMECQPESSISHRFHKDCASRVNNA
                     SYCPHCGEETSKAKEVTIAKADTTSTVTLAPGQEKSLAAEGRADTTTGSIAGAPEDEK
                     SQSTVTQAPECFDPAGPAGFVRPTSGFSQGPGKETLESALIALDSEKPKKLRFHPKQL
                     YFSARQGELQKVLLMLVDGIDPNFKMEHQSKRSPLHAAAEAGHVDICHMLVQAGANID
                     TCSEDQRTPLMEAAENNHLDAVKYLIKAGAQVDPKDAEGSTCLHLAAKKGHYDVVQYL
                     LSNGQMDVNCQDDGGWTPMIWATEYKHVDLVKLLLSKGSDINIRDNEENICLHWAAFS
                     GCVDIAEILLAAKCDLHAVNIHGDSPLHIAARENRYDCVVLFLSRDSDVTLKNKEGET
                     PLQCASLNSQVWSALQMSKALQDSAPDKPVAVEKTVSRDIARGYERIPIPCVNAVDSE
                     LCPTNYKYVSQNCVTSPMNIDRNITHLQYCVCVDDCSSSTCMCGQLSMRCWYDKDGRL
                     LPEFNMAEPPLIFECNHACSCWRNCRNRVVQNGLRARLQLYRTQDMGWGVRSLQDIPL
                     GTFVCEYVGELISDSEADVREEDSYLFDLDNKDGEVYCIDARFYGNVSRFINHHCEPN
                     LVPVRVFMSHQDLRFPRIAFFSTRLIQAGEQLGFDYGERFWDVKGKLFSCRCGSPKCR
                     HSSAALAQRQASAAQEAQENGLPDTSSAAAADPL"
     exon            40..106
                     /gene="Ehmt1"
                     /inference="alignment:Splign:2.1.0"
     exon            107..663
                     /gene="Ehmt1"
                     /inference="alignment:Splign:2.1.0"
     exon            664..835
                     /gene="Ehmt1"
                     /inference="alignment:Splign:2.1.0"
     exon            836..993
                     /gene="Ehmt1"
                     /inference="alignment:Splign:2.1.0"
     exon            994..1182
                     /gene="Ehmt1"
                     /inference="alignment:Splign:2.1.0"
     exon            1183..1260
                     /gene="Ehmt1"
                     /inference="alignment:Splign:2.1.0"
     exon            1261..1381
                     /gene="Ehmt1"
                     /inference="alignment:Splign:2.1.0"
     exon            1382..1519
                     /gene="Ehmt1"
                     /inference="alignment:Splign:2.1.0"
     exon            1520..1665
                     /gene="Ehmt1"
                     /inference="alignment:Splign:2.1.0"
     exon            1666..1809
                     /gene="Ehmt1"
                     /inference="alignment:Splign:2.1.0"
     exon            1810..2036
                     /gene="Ehmt1"
                     /inference="alignment:Splign:2.1.0"
     exon            2037..2204
                     /gene="Ehmt1"
                     /inference="alignment:Splign:2.1.0"
     exon            2205..2287
                     /gene="Ehmt1"
                     /inference="alignment:Splign:2.1.0"
     exon            2288..2394
                     /gene="Ehmt1"
                     /inference="alignment:Splign:2.1.0"
     exon            2395..2517
                     /gene="Ehmt1"
                     /inference="alignment:Splign:2.1.0"
     exon            2518..2619
                     /gene="Ehmt1"
                     /inference="alignment:Splign:2.1.0"
     exon            2620..2724
                     /gene="Ehmt1"
                     /inference="alignment:Splign:2.1.0"
     exon            2725..2879
                     /gene="Ehmt1"
                     /inference="alignment:Splign:2.1.0"
     exon            2880..3047
                     /gene="Ehmt1"
                     /inference="alignment:Splign:2.1.0"
     exon            3048..3192
                     /gene="Ehmt1"
                     /inference="alignment:Splign:2.1.0"
     exon            3193..3270
                     /gene="Ehmt1"
                     /inference="alignment:Splign:2.1.0"
     exon            3271..3386
                     /gene="Ehmt1"
                     /inference="alignment:Splign:2.1.0"
     exon            3387..3473
                     /gene="Ehmt1"
                     /inference="alignment:Splign:2.1.0"
     exon            3474..3552
                     /gene="Ehmt1"
                     /inference="alignment:Splign:2.1.0"
     exon            3553..3728
                     /gene="Ehmt1"
                     /inference="alignment:Splign:2.1.0"
     exon            3729..5017
                     /gene="Ehmt1"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 acgctgcggg cccgggccat ggccgccgcg gatgctgagc aggcagttct ggccaagcaa
       61 gagaccaagc aggattgctg catgaaaact gaactgctaa gggaagatac acttatggct
      121 gctgatgaag gttccacaga gaagcaagca ggagagactc ctatggctgc agatggagaa
      181 accaatgggt cttgtgaaaa gagtggcgat atcagccatc caaatgcacc caaacatact
      241 caggagaaca caagagctag cccacaggaa ggcaccaaca gagtatctcg ggtggcggaa
      301 aatggggttt cagaaagaga cacagaagtg gggaagcaaa accatgttac agctgacgac
      361 ttcatgcaga cgtctgtcat tggaagcaat ggatatttct taaataaacc agccctgcag
      421 ggacagccct tgaggactcc caacactcta aattcctcgc ttcctggtca tgccgcaaaa
      481 acgcttcctg gaggagccag taagtgcagg actccaagtg cacttcctca gacaccaacc
      541 acagcaccca ctgtgcctgg ggaagggagt gcagacacag aggacagaaa gcctacagcc
      601 tcgggcactg atgtcagggt tcacagggca cgcaagacca tgccgaagtc catcctgggc
      661 ctgcacgcag ccagtaaaga ccatagagaa gttcaagacc ataaggaacc aaaagaggat
      721 atcaacagaa acatttctga atgtggacga cagcagcttt taccaacttt cccagccctc
      781 caccagtcgc tacctcagaa tcaatgctac atggccacca caaagtccca gacagcttgc
      841 ttgccttttg ttttagcagc tgcagtgtct cggaagaaaa aacgaagaat gggaacctat
      901 agcttagttc ccaagaaaaa gaccaaagta ttaaagcaga ggacggtgat tgagatgttt
      961 aaaagcatca ctcattccac tgtgggtgcc aagggtgaga aagtcttaga tgatagtgcc
     1021 ctgcatgtaa atggcgagag cttggagatg gactccgagg aggaagactc tgaggagctg
     1081 gaggatgagg aggaccgtgg cgctgagcag gctgctgcat ttcccactga ggacagtagg
     1141 acttctaaag agagcatgtc tgagactgat cgtgctgcaa agatggatgg agattcagag
     1201 gaggaacagg agtctcctga cacaggggag gacgaagacg gtggagatga gtctgacctg
     1261 agttctgaat ctagtatcaa gaagaaattt ctcaagagaa gagggaagac tgacagcccc
     1321 tggatcaaac ctgctcggaa aaggaggcgg agaagcagaa agaagccaag cagcatgctt
     1381 ggttccgagg cttgtaagtc atccccagga agcatggagc aggcagctct gggagacagc
     1441 gctgggtata tggaagtttc tctggattcc ctggatctcc gtgtcagagg aattctgtct
     1501 tcccagacgg aaaacgaagg gctggccaat ggtccggatg tgctggagac ggatggcctc
     1561 catgaagtgc ctctctgcag ctgccgcatg gaaaccccca agagccgtga gatcagcacc
     1621 ctggccaaca accagtgcat ggccactgag agcgtggatc acgaattggg ccggtgtaca
     1681 aacagcgtgg tcaagtatga gctgatgcgc ccttccaaca aagcaccact cttggtgctg
     1741 tgtgaagacc atcggggtcg catggtgaag caccagtgct gtcctggctg cggctacttc
     1801 tgcacggcgg gtaacttcat ggaatgtcag cctgagagca gcatctctca tcgtttccat
     1861 aaggactgtg cctctcgagt caacaatgcc agctactgtc cccattgtgg ggaagagact
     1921 tccaaggcca aagaggtgac catagcaaaa gcagacacga cttccacagt gaccctggcc
     1981 cctggacagg agaagagctt ggctgctgag ggcagggctg acacgaccac tggcagcatt
     2041 gctggagccc cagaggatga aaaatcgcag agcacagtca ctcaggcacc agagtgcttc
     2101 gatcctgcag ggccagctgg tttcgtgagg ccgacctctg gcttttccca gggcccagga
     2161 aaggaaacct tggaaagtgc tctcattgct ctggactctg aaaaaccgaa gaaacttcgc
     2221 ttccacccga agcagctgta cttctccgct aggcagggtg agctgcagaa ggtgcttctc
     2281 atgctggttg atggaattga tcccaacttc aaaatggagc accaaagtaa gcgttcccca
     2341 ttacacgctg ctgcagaggc tggccacgtg gacatctgcc acatgctggt tcaggcgggt
     2401 gccaatattg acacttgctc agaggaccag aggacccccc tgatggaggc cgcagagaac
     2461 aaccacttgg acgcagtgaa gtacctcatc aaggccggag cacaggtgga tccgaaggac
     2521 gcagagggct ccacgtgttt gcacttggct gccaagaaag gccactatga cgtggttcag
     2581 tatctgctgt caaatggaca gatggatgtc aactgccagg atgatggtgg ttggacacct
     2641 atgatctggg ccactgagta caagcacgtg gacctggtga agctgctgct ctctaaaggc
     2701 tctgacatca atatccggga caatgaggag aacatttgtc tgcactgggc agcattttca
     2761 ggctgtgtgg acatagctga aatcctcctg gctgccaagt gtgacctgca tgctgtgaat
     2821 atccacggag actcacccct gcacatcgca gccagggaga atcgctacga ctgtgttgtc
     2881 ctctttcttt ctcgggattc agatgttact ttgaaaaaca aggaaggaga gactcccttg
     2941 cagtgtgcaa gcctcaattc ccaggtgtgg agtgcactgc agatgagcaa ggcacttcag
     3001 gactcagccc ctgacaagcc cgttgctgtt gagaagacgg tgagcagaga cattgcccga
     3061 ggatatgagc gcattcccat tccctgtgtc aatgctgtgg atagtgagtt atgtcccacc
     3121 aactacaagt acgtctccca gaactgcgtg acgtccccaa tgaacatcga caggaacata
     3181 actcatttgc agtactgcgt ttgtgtagac gactgctcct ctagcacctg catgtgtggc
     3241 cagctgagca tgcgctgctg gtatgacaag gacgggcgac ttctgcctga gtttaacatg
     3301 gcagaaccac ccttgatctt cgagtgcaat catgcctgct cctgctggag gaactgccgc
     3361 aatcgcgtgg tgcagaatgg tctcagggca aggctgcagc tttatcggac acaggacatg
     3421 ggctggggtg tgcggtccct tcaggatatc ccgctgggca cctttgtctg cgaatacgtg
     3481 ggggagctga tttcagactc cgaggctgat gttcgggaag aggactctta cctctttgat
     3541 cttgacaata aggatggaga ggtgtactgc attgatgctc ggttctacgg gaatgtcagc
     3601 cggttcataa accaccactg cgagcccaac cttgtgcctg tgcgagtgtt catgtcacac
     3661 caggacctac ggtttcccag gattgccttc ttcagcaccc gcctgattca ggctggggaa
     3721 cagctggggt ttgactatgg ggaacgcttt tgggacgtca agggcaagct cttcagttgc
     3781 cggtgtgggt ctcccaagtg tcggcactca agtgcagccc tggcccagcg gcaagccagt
     3841 gcagcccagg aggctcagga gaatggcctt ccagatacca gctctgcagc tgctgctgac
     3901 cccctatgaa ttgacagtgg ggccagtggg accctcaaaa tcaggttctg gtgactgcaa
     3961 tttagggagg aagaaagtct gcacatgtcc aagaggagct ggaggtgtgg acacctccca
     4021 ggaatgttct gagcctgagg cgttgatctc aactggccga gtgctcaggg cagagcatgc
     4081 ctctggtcct gcacctcaga gacctgggac tccatgttgc ataactcgtg agttattggt
     4141 ggtgactctg ctgcagcgct tcccacgtct ccctttggtc tccgaggccc ttgctccact
     4201 gccagttttg tttgtatact gtctggtcat gctcctgtat ggcactcacc gaggccaccg
     4261 tcacctacaa gctgctccgt taggcctgct gcccacagcc tgtgttgctg gcaccagccc
     4321 ccgtgtgcca ctggaggaca ctagctccct cagccctggg gagtttggtg acaccactca
     4381 ctgacgaact gtgggtttgc tttttaaaga gaccccgtat gtagccctat atatgaagcc
     4441 tcttactggt gtttctttgc aaggaagaag gttggtgtgt gcaggcagac cctgcattgg
     4501 cccagccagt tccgttgacg caggcacctt ttgttgattt ctaagccaca tgttatgatg
     4561 atggataaac tcgtttgttt tttaccatta ctgaacatta ggacaaacaa ggaataaaaa
     4621 cacaaaacac agacaacggt gctgaatctg gtgtggtttc tactcaccac atgaaaataa
     4681 actatcaact gtaaaaaaag aacaaaatga ttttagaata aagtgcagga aaaacttttt
     4741 taaaatgtta gtcttgtagt tgaataaatt tgccatcacc ttttgtgtga tggcctggca
     4801 ggtcatgtac ttttttttgg catatacctt tctaaatact gtaattagtg cagcaacagt
     4861 ggtttttttg gtgtgactct tctaaaacat tcataatgca gtcatgttta tttttttctg
     4921 ttaaaatgtt ttttgacagt tttaagagca gtcttttggc tcagaccatt tcttgttctg
     4981 ttttcaatga aatcaataaa aaaaaaagta ctttaaa
//
