LOCUS       NM_133606               3099 bp    mRNA    linear   ROD 17-FEB-2024
DEFINITION  Rattus norvegicus enoyl-CoA hydratase and 3-hydroxyacyl CoA
            dehydrogenase (Ehhadh), mRNA.
ACCESSION   NM_133606
VERSION     NM_133606.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 3099)
  AUTHORS   Kasaragod,P., Schmitz,W., Hiltunen,J.K. and Wierenga,R.K.
  TITLE     The isomerase and hydratase reaction mechanism of the crotonase
            active site of the multifunctional enzyme (type-1), as deduced from
            structures of complexes with 3S-hydroxy-acyl-CoA
  JOURNAL   FEBS J 280 (13), 3160-3175 (2013)
   PUBMED   23351063
  REMARK    GeneRIF: Data from crystallographic binding studies suggest that a
            catalytic water molecule, bound between two catalytic glutamates
            (Glu103/Glu123), plays important role in enoyl-CoA isomerase and
            enoyl-CoA hydratase reaction mechanisms of MFE1.
REFERENCE   2  (bases 1 to 3099)
  AUTHORS   Kasaragod,P., Venkatesan,R., Kiema,T.R., Hiltunen,J.K. and
            Wierenga,R.K.
  TITLE     Crystal structure of liganded rat peroxisomal multifunctional
            enzyme type 1: a flexible molecule with two interconnected active
            sites
  JOURNAL   J Biol Chem 285 (31), 24089-24098 (2010)
   PUBMED   20463028
  REMARK    GeneRIF: liganded rat peroxisomal multifunctional enzyme type 1 is
            a flexible molecule with two interconnected active sites [rpMFE1]
REFERENCE   3  (bases 1 to 3099)
  AUTHORS   Ghosh,D. and Berg,J.M.
  TITLE     A proteome-wide perspective on peroxisome targeting signal
            1(PTS1)-Pex5p affinities
  JOURNAL   J Am Chem Soc 132 (11), 3973-3979 (2010)
   PUBMED   20178365
REFERENCE   4  (bases 1 to 3099)
  AUTHORS   Zhao,S., Xu,W., Jiang,W., Yu,W., Lin,Y., Zhang,T., Yao,J., Zhou,L.,
            Zeng,Y., Li,H., Li,Y., Shi,J., An,W., Hancock,S.M., He,F., Qin,L.,
            Chin,J., Yang,P., Chen,X., Lei,Q., Xiong,Y. and Guan,K.L.
  TITLE     Regulation of cellular metabolism by protein lysine acetylation
  JOURNAL   Science 327 (5968), 1000-1004 (2010)
   PUBMED   20167786
REFERENCE   5  (bases 1 to 3099)
  AUTHORS   Dirkx,R., Meyhi,E., Asselberghs,S., Reddy,J., Baes,M. and Van
            Veldhoven,P.P.
  TITLE     Beta-oxidation in hepatocyte cultures from mice with peroxisomal
            gene knockouts
  JOURNAL   Biochem Biophys Res Commun 357 (3), 718-723 (2007)
   PUBMED   17442273
REFERENCE   6  (bases 1 to 3099)
  AUTHORS   Chen,G.L., Balfe,A., Erwa,W., Hoefler,G., Gaertner,J., Aikawa,J.
            and Chen,W.W.
  TITLE     Import of human bifunctional enzyme into peroxisomes of human
            hepatoma cells in vitro
  JOURNAL   Biochem Biophys Res Commun 178 (3), 1084-1091 (1991)
   PUBMED   1651711
REFERENCE   7  (bases 1 to 3099)
  AUTHORS   Palosaari,P.M. and Hiltunen,J.K.
  TITLE     Peroxisomal bifunctional protein from rat liver is a trifunctional
            enzyme possessing 2-enoyl-CoA hydratase, 3-hydroxyacyl-CoA
            dehydrogenase, and delta 3, delta 2-enoyl-CoA isomerase activities
  JOURNAL   J Biol Chem 265 (5), 2446-2449 (1990)
   PUBMED   2303409
REFERENCE   8  (bases 1 to 3099)
  AUTHORS   Litwin,J.A., Volkl,A., Muller-Hocker,J. and Fahimi,H.D.
  TITLE     Immunocytochemical demonstration of peroxisomal enzymes in human
            kidney biopsies
  JOURNAL   Virchows Arch B Cell Pathol Incl Mol Pathol 54 (4), 207-213 (1988)
   PUBMED   2895531
REFERENCE   9  (bases 1 to 3099)
  AUTHORS   Ishii,N., Hijikata,M., Osumi,T. and Hashimoto,T.
  TITLE     Structural organization of the gene for rat enoyl-CoA
            hydratase:3-hydroxyacyl-CoA dehydrogenase bifunctional enzyme
  JOURNAL   J Biol Chem 262 (17), 8144-8150 (1987)
   PUBMED   3036802
REFERENCE   10 (bases 1 to 3099)
  AUTHORS   Osumi,T., Ishii,N., Hijikata,M., Kamijo,K., Ozasa,H., Furuta,S.,
            Miyazawa,S., Kondo,K., Inoue,K., Kagamiyama,H. et al.
  TITLE     Molecular cloning and nucleotide sequence of the cDNA for rat
            peroxisomal enoyl-CoA: hydratase-3-hydroxyacyl-CoA dehydrogenase
            bifunctional enzyme
  JOURNAL   J Biol Chem 260 (15), 8905-8910 (1985)
   PUBMED   4019459
COMMENT     PROVISIONAL REFSEQ: This record has not yet been subject to final
            NCBI review. The reference sequence was derived from
            JACYVU010000222.1.
            
            [WARNING] On Apr 3, 2024 this sequence was replaced by NM_133606.3.
            
            On Nov 27, 2020 this sequence version replaced NM_133606.1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: K03249.1, BC089777.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMD00132298, SAMD00132300
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-95                JACYVU010000222.1  58551701-58551795
            96-199              JACYVU010000222.1  58562389-58562492
            200-369             JACYVU010000222.1  58566327-58566496
            370-481             JACYVU010000222.1  58568435-58568546
            482-586             JACYVU010000222.1  58571713-58571817
            587-928             JACYVU010000222.1  58579547-58579888
            929-3099            JACYVU010000222.1  58582777-58584947
FEATURES             Location/Qualifiers
     source          1..3099
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="BN"
                     /db_xref="taxon:10116"
                     /chromosome="11"
                     /map="11q23"
     gene            1..3099
                     /gene="Ehhadh"
                     /gene_synonym="Lbp; MEF; Mfe; Mfe1; MFP1; Pbe; Pbfe;
                     pe-CoA; perMFE-1"
                     /note="enoyl-CoA hydratase and 3-hydroxyacyl CoA
                     dehydrogenase"
                     /db_xref="GeneID:171142"
                     /db_xref="RGD:621441"
     exon            1..95
                     /gene="Ehhadh"
                     /gene_synonym="Lbp; MEF; Mfe; Mfe1; MFP1; Pbe; Pbfe;
                     pe-CoA; perMFE-1"
                     /inference="alignment:Splign:2.1.0"
     CDS             22..2190
                     /gene="Ehhadh"
                     /gene_synonym="Lbp; MEF; Mfe; Mfe1; MFP1; Pbe; Pbfe;
                     pe-CoA; perMFE-1"
                     /EC_number="1.1.1.35"
                     /EC_number="5.3.3.8"
                     /EC_number="4.2.1.17"
                     /note="peroxisomal bifunctional enzyme type 1; peroxisomal
                     enoyl-CoA/hydrotase-3-hydroxyacyl-CoA; enoyl-CoA,
                     hydratase/3-hydroxyacyl CoA dehydrogenase; L-specific
                     bifunctional protein; multifunctional enzyme type 1;
                     enoyl-Coenzyme A, hydratase/3-hydroxyacyl Coenzyme A
                     dehydrogenase; multifunctional enzyme 1; multifunctional
                     protein 1"
                     /codon_start=1
                     /product="peroxisomal bifunctional enzyme"
                     /protein_id="NP_598290.1"
                     /db_xref="GeneID:171142"
                     /db_xref="RGD:621441"
                     /translation="MAEYLRLPHSLAMIRLCNPPVNAVSPTVIREVRNGLQKAGSDHT
                     VKAIVICGANGNFCAGADIHGFSAFTPGLALGSLVDEIQRYQKPVLAAIQGVALGGGL
                     ELALGCHYRIANAKARVGLPEVTLGILPGARGTQLLPRVVGVPVALDLITSGKYLSAD
                     EALRLGILDAVVKSDPVEEAIKFAQKIIDKPIEPRRIFNKPVPSLPNMDSVFAEAIAK
                     VRKQYPGVLAPETCVRSIQASVKHPYEVGIKEEEKLFMYLRASGQAKALQYAFFAEKS
                     ANKWSTPSGASWKTASAQPVSSVGVLGLGTMGRGIAISFARVGISVVAVESDPKQLDA
                     AKKIITFTLEKEASRAHQNGQASAKPKLRFSSSTKELSTVDLVVEAVFEDMNLKKKVF
                     AELSALCKPGAFLCTNTSALNVDDIASSTDRPQLVIGTHFFSPAHVMRLLEVIPSRYS
                     SPTTIATVMSLSKKIGKIGVVVGNCYGFVGNRMLAPYYNQGFFLLEEGSKPEDVDGVL
                     EEFGFKMGPFRVSDLAGLDVGWKIRKGQGLTGPSLPPGTPVRKRGNSRYSPLGDMLCE
                     AGRFGQKTGKGWYQYDKPLGRIHKPDPWLSTFLSQYREVHHIEQRTISKEEILERCLY
                     SLINEAFRILEEGMAARPEHIDVIYLHGYGWPRHKGGPMFYAASVGLPTVLEKLQKYY
                     RQNPDIPQLEPSDYLRRLVAQGSPPLKEWQSLAGPHGSKL"
     exon            96..199
                     /gene="Ehhadh"
                     /gene_synonym="Lbp; MEF; Mfe; Mfe1; MFP1; Pbe; Pbfe;
                     pe-CoA; perMFE-1"
                     /inference="alignment:Splign:2.1.0"
     exon            200..369
                     /gene="Ehhadh"
                     /gene_synonym="Lbp; MEF; Mfe; Mfe1; MFP1; Pbe; Pbfe;
                     pe-CoA; perMFE-1"
                     /inference="alignment:Splign:2.1.0"
     exon            370..481
                     /gene="Ehhadh"
                     /gene_synonym="Lbp; MEF; Mfe; Mfe1; MFP1; Pbe; Pbfe;
                     pe-CoA; perMFE-1"
                     /inference="alignment:Splign:2.1.0"
     exon            482..586
                     /gene="Ehhadh"
                     /gene_synonym="Lbp; MEF; Mfe; Mfe1; MFP1; Pbe; Pbfe;
                     pe-CoA; perMFE-1"
                     /inference="alignment:Splign:2.1.0"
     exon            587..928
                     /gene="Ehhadh"
                     /gene_synonym="Lbp; MEF; Mfe; Mfe1; MFP1; Pbe; Pbfe;
                     pe-CoA; perMFE-1"
                     /inference="alignment:Splign:2.1.0"
     exon            929..3099
                     /gene="Ehhadh"
                     /gene_synonym="Lbp; MEF; Mfe; Mfe1; MFP1; Pbe; Pbfe;
                     pe-CoA; perMFE-1"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 ggtgcaggat accttgagaa aatggctgag tatctgaggc tgccccactc cctggcgatg
       61 atccgcctct gcaacccgcc agtcaacgcc gtcagtccaa ctgtaatcag ggaagtaaga
      121 aatggactcc agaaagctgg atcagaccac acggttaaag ccattgtgat ctgtggcgca
      181 aacggaaact tctgtgcagg tgctgatatc catggcttta gtgcttttac ccctggcctt
      241 gcattaggaa gcttggtaga tgaaatacag agataccaga agccggtgtt ggccgctatc
      301 caaggtgtgg ctctcggagg aggactggag ctggccttgg gctgtcacta tcggattgcc
      361 aatgcaaagg ctcgtgtcgg cttgccagaa gtcacactgg ggattcttcc tggtgcaaga
      421 ggaacccagc ttctccccag ggtcgttgga gttcctgttg ctcttgacct aattacctca
      481 ggaaaatatc tttcagcaga tgaagcactc aggcttggaa ttctggatgc agtcgtgaag
      541 tcagacccag ttgaagaagc catcaaattt gctcagaaga ttatagataa acccatagaa
      601 ccccgcagga tctttaacaa gccagttcca agcttgccca acatggacag tgtttttgca
      661 gaagccatcg ccaaggtacg aaaacagtac cctggtgtcc tggctccgga gacgtgtgtc
      721 cgctcaatcc aggcctctgt gaagcatccc tacgaagtag gcatcaagga agaggaaaag
      781 ctgtttatgt acctccgggc atccgggcag gctaaagccc tacagtatgc cttctttgct
      841 gaaaagtctg caaataagtg gtcaactccc tcaggagcgt cttggaaaac agcctctgct
      901 caacccgtct cctcagttgg cgttcttggc ttgggaacga tgggccgagg catcgccatt
      961 tcttttgcga gagtggggat ctctgtggtt gctgtggagt cagacccaaa gcagctagat
     1021 gctgcaaaga agataatcac tttcaccttg gagaaggaag catccagagc gcatcagaac
     1081 ggccaagctt cggcaaaacc aaaactcagg ttctcctcat ccacaaagga actttcaact
     1141 gtggatttgg tggttgaagc agtgttcgaa gacatgaacc tgaagaaaaa ggtctttgct
     1201 gagctgtcag ccctgtgcaa gccaggagcc tttctgtgca ccaatacctc ggcgctgaac
     1261 gtggacgaca ttgcttcttc cacagatcgc cctcagctgg tgattggcac ccacttcttc
     1321 tcaccagccc atgtcatgag gttgctagag gtcattccta gccgatactc ttcccctact
     1381 accatcgcca cggttatgag cttgtccaaa aagatcggaa agattggagt agtggttggc
     1441 aactgctatg gatttgttgg gaatcggatg ttggctccct attacaacca ggggtttttc
     1501 ttgttagagg aaggtagcaa gccagaggat gtagacgggg tcttggaaga gtttggtttt
     1561 aaaatgggac ccttcagagt gtcagacctc gcagggctag atgtgggttg gaaaattcgc
     1621 aaggggcaag gccttactgg accatcattg ccaccaggga cccccgtccg aaagaggggc
     1681 aacagcaggt actccccact tggcgatatg ctctgtgaag ctgggcggtt tggtcagaag
     1741 acaggtaaag gctggtatca gtatgacaag ccactgggtc gcatccacaa acctgatccc
     1801 tggctttcta cgttcctgtc acaatataga gaggttcacc acatcgagca gcgcaccatc
     1861 agcaaggagg agatcctgga gcgttgctta tattccctca tcaatgaggc gttccgcatc
     1921 ttggaggagg ggatggctgc tcgcccagag cacattgatg tcatctactt gcacgggtac
     1981 gggtggccaa ggcacaaggg cgggcccatg ttctatgctg cctcagttgg gttgcccaca
     2041 gttctagaga aactgcagaa atattacagg cagaaccctg acatccccca gctggagccc
     2101 agtgactacc tcagaaggct ggtagcccag ggaagccctc ctctgaagga atggcaaagc
     2161 ttggcagggc cccacggcag caaactgtga ttcagccttc tgcgttttgc cacgtatgtt
     2221 ggcacctaat ttcagtatga ttccttccta aaatccaaac agatcgctct gaagtacaaa
     2281 tcatgattgc tgctgctgct gctgctgata gctggtcagt aaaagctcct ctctgtatct
     2341 ttctacctca taattccaat ggtcagactt caggaatgtc tacagttagt ctatacctct
     2401 gactgcactt ctatcggaaa aattcaacga atgtatgttt actactacaa gcattcttct
     2461 cttttgactg tttgcccact ctaacaaaaa cgtatacaaa atgttgctaa tagaataatt
     2521 cacacctcca gttgacaacc caaatggcct tagcagatca acacaggtgg catatacacg
     2581 cacttgacac attccagctg tgtgaagtta ctgccacctg ttgtaacatg gtgctgggtt
     2641 agagcactgt tctgagtgag gggaggcagg cagagaaggc aggcagataa ggccacacat
     2701 catatggatg tggatataaa attccaggat atgcaggtct gtggacaaca tagatgagta
     2761 gcccaaggct gtaactgttc atacatgcat atattttttg gtcctttttt ttttttcgga
     2821 gctggggacc gaacccaggg ccttgcgctt cctaggtaag cgctctaacc actgagctaa
     2881 atccccagcc ccgaggctgt aactgttaga agttaaaaat cactactgat tagtacagag
     2941 tatctttggg agctgataaa attttctaaa attactctag tggtggatct gtaacacatt
     3001 gagttcaatg gttcaaagtg aattatattt tatatatata tatatatata tatatatata
     3061 tatatatata tagtctatct caataaagtt aataaaatt
//
