LOCUS       NM_178334               3560 bp    mRNA    linear   ROD 13-FEB-2025
DEFINITION  Rattus norvegicus egl-9 family hypoxia-inducible factor 1 (Egln1),
            mRNA.
ACCESSION   NM_178334 NR_003956 XM_039098215
VERSION     NM_178334.4
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 3560)
  AUTHORS   Ju,S., Lim,L., Wi,K., Park,C., Ki,Y.J., Choi,D.H. and Song,H.
  TITLE     LRP5 Regulates HIF-1alpha Stability via Interaction with PHD2 in
            Ischemic Myocardium
  JOURNAL   Int J Mol Sci 22 (12), 6581 (2021)
   PUBMED   34205318
  REMARK    GeneRIF: LRP5 Regulates HIF-1alpha Stability via Interaction with
            PHD2 in Ischemic Myocardium.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3560)
  AUTHORS   Sun,Z., Xie,Y., Lee,R.J., Chen,Y., Jin,Q., Lv,Q., Wang,J., Yang,Y.,
            Li,Y., Cai,Y., Wang,R., Han,Z., Zhang,L. and Xie,M.
  TITLE     Myocardium-targeted transplantation of PHD2 shRNA-modified bone
            mesenchymal stem cells through ultrasound-targeted microbubble
            destruction protects the heart from acute myocardial infarction
  JOURNAL   Theranostics 10 (11), 4967-4982 (2020)
   PUBMED   32308762
  REMARK    GeneRIF: Myocardium-targeted transplantation of PHD2 shRNA-modified
            bone mesenchymal stem cells through ultrasound-targeted microbubble
            destruction protects the heart from acute myocardial infarction.
            Erratum:[Theranostics. 2022 Mar 6;12(6):2637-2638. doi:
            10.7150/thno.72333.. PMID: 35401840]
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 3560)
  AUTHORS   Chen,H., Zhang,H., Zheng,Y., Min,X., Luo,Y., Zhou,W., Ma,F., Li,J.,
            Lu,Q., Zhang,C., Cai,H. and Sha,W.
  TITLE     Prolyl hydroxylase 2 silencing enhances the paracrine effects of
            mesenchymal stem cells on necrotizing enterocolitis in an
            NF-kappaB-dependent mechanism
  JOURNAL   Cell Death Dis 11 (3), 188 (2020)
   PUBMED   32179740
  REMARK    GeneRIF: Prolyl hydroxylase 2 silencing enhances the paracrine
            effects of mesenchymal stem cells on necrotizing enterocolitis in
            an NF-kappaB-dependent mechanism.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 3560)
  AUTHORS   Xiong,Y., Xia,Y., Deng,J., Yan,X., Ke,J., Zhan,J., Zhang,Z. and
            Wang,Y.
  TITLE     Direct Peritoneal Resuscitation with Pyruvate Protects the Spinal
            Cord and Induces Autophagy via Regulating PHD2 in a Rat Model of
            Spinal Cord Ischemia-Reperfusion Injury
  JOURNAL   Oxid Med Cell Longev 2020, 4909103 (2020)
   PUBMED   31998438
  REMARK    GeneRIF: he above results indicated that direct peritoneal
            resuscitation with pyruvate showed effective protection to
            ischemia-reperfusion of the spinal cord through activating
            autophagy via acting on PHD2 and its downstream HIF-1alpha/BNIP3
            pathway.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 3560)
  AUTHORS   Rane,A., Rajagopalan,S., Ahuja,M., Thomas,B., Chinta,S.J. and
            Andersen,J.K.
  TITLE     Hsp90 Co-chaperone p23 contributes to dopaminergic mitochondrial
            stress via stabilization of PHD2: Implications for Parkinson's
            disease
  JOURNAL   Neurotoxicology 65, 166-173 (2018)
   PUBMED   29471019
  REMARK    GeneRIF: Our data suggests that mitochondrial stress-mediated
            elevations in PHD2 interaction with the p23-hsp90 complex have
            detrimental effects on the survival of DAergic neurons, while p23
            inhibition is neuroprotective.
REFERENCE   6  (bases 1 to 3560)
  AUTHORS   Hirsila,M., Koivunen,P., Gunzler,V., Kivirikko,K.I. and
            Myllyharju,J.
  TITLE     Characterization of the human prolyl 4-hydroxylases that modify the
            hypoxia-inducible factor
  JOURNAL   J Biol Chem 278 (33), 30772-30780 (2003)
   PUBMED   12788921
REFERENCE   7  (bases 1 to 3560)
  AUTHORS   Metzen,E., Berchner-Pfannschmidt,U., Stengel,P., Marxsen,J.H.,
            Stolze,I., Klinger,M., Huang,W.Q., Wotzlaw,C., Hellwig-Burgel,T.,
            Jelkmann,W., Acker,H. and Fandrey,J.
  TITLE     Intracellular localisation of human HIF-1 alpha hydroxylases:
            implications for oxygen sensing
  JOURNAL   J Cell Sci 116 (Pt 7), 1319-1326 (2003)
   PUBMED   12615973
REFERENCE   8  (bases 1 to 3560)
  AUTHORS   Straub,J.A., Lipscomb,E.A., Yoshida,E.S. and Freeman,R.S.
  TITLE     Induction of SM-20 in PC12 cells leads to increased cytochrome c
            levels, accumulation of cytochrome c in the cytosol, and
            caspase-dependent cell death
  JOURNAL   J Neurochem 85 (2), 318-328 (2003)
   PUBMED   12675908
REFERENCE   9  (bases 1 to 3560)
  AUTHORS   Bruick,R.K. and McKnight,S.L.
  TITLE     A conserved family of prolyl-4-hydroxylases that modify HIF
  JOURNAL   Science 294 (5545), 1337-1340 (2001)
   PUBMED   11598268
REFERENCE   10 (bases 1 to 3560)
  AUTHORS   Lipscomb,E.A., Sarmiere,P.D., Crowder,R.J. and Freeman,R.S.
  TITLE     Expression of the SM-20 gene promotes death in nerve growth
            factor-dependent sympathetic neurons
  JOURNAL   J Neurochem 73 (1), 429-432 (1999)
   PUBMED   10386996
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            JAXUCZ010000019.1.
            
            On or before Jan 31, 2022 this sequence version replaced
            XM_039098215.1, NM_178334.3.
            
            Summary: This gene encodes a component of a transcriptional complex
            that plays a central role in mammalian oxygen homeostasis. Hypoxia
            reduces the activity of prolyl hyxroxylases that hydroxylate
            specific proline residues of the hypoxia-inducible factor-1a
            (Hif1a). In the absence of hydroxylation, the Hif1a transcription
            factor accumulates and activates transcription of
            hypoxia-responsive target genes. This gene encodes one of the three
            known Hif-interacting 2-oxoglutarate/iron-dependent
            prolyl-hydroxylases (HIF-PHDs) in rat. Targeted disruption of this
            gene in mice produced embryonic lethality between embryonic day
            12.5 and day 14.5. Based on the transcript data currently available
            for rat, this Reference Sequence is believed to contain the
            complete coding region for this gene. However, when compared to its
            mouse and human orthologs, it has a shorter 5' coding region and an
            incomplete N-terminus zf-MYND domain. This locus currently has
            limited transcript data and aligns to an unfinished region of the
            rat reference genome assembly. It is therefore uncertain whether
            its coding region can be extended at the 5' end to encode a
            complete zf-MYND domain, whether no further changes need to be made
            to its coding region, or whether it is a transcribed pseudogene
            that does not encode a functional protein. As more transcript and
            experimental data become available, the coding status of this locus
            may change. [provided by RefSeq, Jul 2008].
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC081694.1, SRR26643295.58552.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN12840107 [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-1095              JAXUCZ010000019.1  69803587-69804681   c
            1096-1215           JAXUCZ010000019.1  69772511-69772630   c
            1216-1352           JAXUCZ010000019.1  69770970-69771106   c
            1353-1420           JAXUCZ010000019.1  69768795-69768862   c
            1421-3560           JAXUCZ010000019.1  69765276-69767415   c
FEATURES             Location/Qualifiers
     source          1..3560
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="BN"
                     /db_xref="taxon:10116"
                     /chromosome="19"
                     /map="19q12"
     gene            1..3560
                     /gene="Egln1"
                     /gene_synonym="HIF-PH2; HPH-2; PHD-2; PHD2"
                     /note="egl-9 family hypoxia-inducible factor 1"
                     /db_xref="GeneID:308913"
                     /db_xref="RGD:631375"
     exon            1..1095
                     /gene="Egln1"
                     /gene_synonym="HIF-PH2; HPH-2; PHD-2; PHD2"
                     /inference="alignment:Splign:2.1.0"
     CDS             271..1476
                     /gene="Egln1"
                     /gene_synonym="HIF-PH2; HPH-2; PHD-2; PHD2"
                     /EC_number="1.14.11.29"
                     /note="hypoxia-inducible factor prolyl hydroxylase 2;
                     HIF-prolyl hydroxylase 2; prolyl hydroxylase
                     domain-containing protein 2"
                     /codon_start=1
                     /product="egl nine homolog 1"
                     /protein_id="NP_848017.2"
                     /db_xref="GeneID:308913"
                     /db_xref="RGD:631375"
                     /translation="MASDSGGPGVLSASERDRQYCELCGKMENLLRCGRCRSSFYCCK
                     EHQRQDWKKHKLVCQGGEAPRAQPAPAQPRVAPPPGGAPGAARAGGAARRGDSSTAAS
                     RVPGPEDATQAGSGPGPAEPSSEDPPPSRSPGPERASLCPAGGGPGEALSPSGGLRPN
                     GQTKPLPALKLALEYIVPCMNKHGICVVDDFLGRETGQQIGDEVRALHDTGKFTDGQL
                     VSQKSDSSKDIRGDKITWIEGKEPGCETIGLLMSSMDDLIRHCSGKLGNYRINGRTKA
                     MVACYPGNGTGYVRHVDNPNGDGRCVTCIYYLNKDWDAKVSGGILRIFPEGKAQFADI
                     EPKFDRLLFFWSDRRNPHEVQPAYATRYAITVWYFDADERARAKVKYLTGEKGVRVEL
                     KPNSVSKDV"
     exon            1096..1215
                     /gene="Egln1"
                     /gene_synonym="HIF-PH2; HPH-2; PHD-2; PHD2"
                     /inference="alignment:Splign:2.1.0"
     exon            1216..1352
                     /gene="Egln1"
                     /gene_synonym="HIF-PH2; HPH-2; PHD-2; PHD2"
                     /inference="alignment:Splign:2.1.0"
     exon            1353..1420
                     /gene="Egln1"
                     /gene_synonym="HIF-PH2; HPH-2; PHD-2; PHD2"
                     /inference="alignment:Splign:2.1.0"
     exon            1421..3560
                     /gene="Egln1"
                     /gene_synonym="HIF-PH2; HPH-2; PHD-2; PHD2"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 gtacgtgcag cgcgcgcgga gcgagtggcg cccgcaggcc cgcgccctgc ccgcccgccc
       61 cgacgcagcc ggctgggccc gcccgcccag ggcgccgtgc gccgcgcagg ccgcgctctc
      121 tccggcgcga tgcggcgcta ggcggcccgg ggcgaggcag gcgaggccag ggcgcgcgcg
      181 gcctcccgca gcgggcggcg gccccgggcg ggcgccccga cggccccgcc gccgccccgc
      241 tcccggccgg cggcccgccc tgccgcggcc atggccagtg acagcggcgg gcccggcgtg
      301 ctgagcgcca gcgagcgcga ccggcagtac tgcgagctgt gcgggaagat ggagaacctg
      361 ctgcgctgcg gccgctgccg cagctccttc tactgctgca aggagcacca gcgccaggac
      421 tggaagaagc acaagctggt gtgccagggc ggcgaggccc cccgcgcgca gcccgcgcca
      481 gcgcagcccc gcgtcgcgcc cccgcccggt ggggcccccg gagccgcgcg cgccggcggg
      541 gcggcccggc gaggggacag cagcacggcg gcctcgcgcg taccgggccc ggaggacgcg
      601 acgcaggccg ggagcggccc cggcccggca gagcccagct ccgaggatcc tccgcctagc
      661 aggtctccgg gccccgagcg cgctagcttg tgcccagcgg gcggcggccc cggggaggcg
      721 ctgagtccca gcggagggct gcggcccaac gggcagacca agccgctgcc cgcgctgaag
      781 ctggcgctgg agtacatcgt gccgtgcatg aacaagcacg gcatctgcgt ggtggacgac
      841 ttcctgggca gggagaccgg gcagcagatc ggcgacgagg tgcgcgccct gcacgacacc
      901 ggcaagttca cggacgggca actggtcagc cagaagagtg actcttccaa ggacatccgg
      961 ggggacaaga tcacctggat cgagggcaaa gagcccggct gcgagaccat cggcctgctc
     1021 atgagcagca tggacgacct gatccgccac tgcagcggga agctgggcaa ctacaggata
     1081 aacggccgaa cgaaagccat ggtcgcctgt tacccaggca acgggacagg ctacgtccgt
     1141 cacgtcgata acccaaatgg agacggaaga tgtgtgacat gtatatatta tctaaataaa
     1201 gactgggacg ccaaggtgag cggaggtatt cttcgaattt tcccagaagg caaagcccag
     1261 tttgctgaca ttgaacccaa gtttgataga ttgctgtttt tctggtctga ccggcgtaac
     1321 cctcatgaag tacagcctgc atacgccaca aggtacgcaa taaccgtttg gtatttcgat
     1381 gcagacgagc gagcaagagc taaagtaaaa tatctcacag gtgagaaagg tgtgagggtt
     1441 gaactcaagc ccaattcagt cagcaaagat gtctagcagg gccttgggtc cagcagtacc
     1501 cgcgtcacct acagcctctc agttgccttc tgtggacccg tggacatgtg gccaggatga
     1561 acagagacac ctgcctggca tttcatctgg gagccaggct acttcgccag gtgtcatcca
     1621 acagagggct ccatctgccg ggactgtact gtggggtcag ctccagatct gtgactgctc
     1681 ttggccgctg actgaagagg agacactgtc ggaggagagt agcttttcca tctggacaca
     1741 aaacaagggc cctttgtagg attttcttca gtcttctatt ttgccagacc tgtcacctaa
     1801 ctgagttcat ttcatctctt ttttatatca agtttgaatt cggggaattt ttgtattagg
     1861 tacaatttat caaaactgaa ttaagaaaaa aatttacagt attattctca aaataacatc
     1921 aatctatttt tgtaaacctc ttcatgctat taaattttgc cctaaaggcc tcctacgatg
     1981 attgtcgcca gtgaatgatg attccgtttc ttctgcctga aagtaaagga agggccctga
     2041 gtcctggctc acgtgcgcga ggtttttctt ggcccgcctc tcggtgattc gaactcacct
     2101 ccgctgccct cacgctccga cctaacagtg gatggtcgcc accggtgccc aactaactca
     2161 tagttccgag aggaaacaag ccacaggaaa aggctctgtg tcttcggtta atttttttta
     2221 aagctaagtg acgggcgcta aatgagctcc tcctcctcgg cttcgggttc ccaagacaaa
     2281 gggctccttc tgcgtcaggt ctagcctgtc gccggccttg tctcacagct gcaggtttcc
     2341 ctgtggcttc tccttagttt gactgtgctt tccagaccct tccaggtcag ggccttgttc
     2401 ttgcagcaag gcacctgtgg ccccaggcac atggctgtgt gcgtgtggtc cttggctccc
     2461 tggacggcca tgaggcactc tcataaccag taatgaaagc cacgtgtgcc agcttagaga
     2521 gcaagaggtg taaaggcaca ctctctgttt catgggtttt tctcttttta gaggacagag
     2581 ggacaaccac acaaggccgc cagactgctg tcacctctac ggtcccctta gccagagttt
     2641 gcacagatca tgggtgtaac cccagtcccc ttaggtgtta cgtctccgac cccggtcttt
     2701 gccaagacgt gcgtatacag aactatttct gtgtgtctcc ttcataccag acttagcacc
     2761 cacgcagttc atacccagtt agaaaccttt tctatgggaa gctcagcccg tcttaagaga
     2821 ttgtcattgt tcatataaaa catgaacaca gccaagtaaa gctgagagtg gacagggctt
     2881 actcagcact gtgctcgcat gaggggctca cggcaggaag tccactcctg acccacatcc
     2941 gctgaccact cctctccagc aagtgcctct gcagccgggc cagctcaagg tttggccgcc
     3001 cggctccgaa gcaccgtgtt tcacatttgg gaggaactgt ttggccttga tgcacttggt
     3061 ctgattgact ggagcagcag accctggagt cctttgtatc tcagactttt actgatgtac
     3121 caggtcccag gttctgctgc aggggatggg gcggggtgct cgcgccaggc aaaatttaaa
     3181 ttctatatct tttggaaagt gcgtgctttc ctggaggtca ctgtgagaaa caacaacaag
     3241 aaatcaggct agctaccccc ttaaaatgac cacttaaact caatttcatg agcaggattc
     3301 tatttaaaac catttgtggc ccatatgtgt tcgttctggt gagagagaaa gctttatgat
     3361 tctgtttctg ttcaacacct cagccagtcg tggaacagcc ctctatctac gacaggcagg
     3421 gcacttcagg gttcgcagag agcctcatgt ggtttggaag tggtgtttcc tatgaaagcc
     3481 tctcacgttg cttgtaaagc taatctaatt aaaaagatgt ataaatgttc ttgaaaaaaa
     3541 aaaaaagcta actgttgtca
//
