LOCUS       NM_001165880            1449 bp    mRNA    linear   ROD 30-MAR-2024
DEFINITION  Rattus norvegicus EGF-like-domain, multiple 8 (Egfl8), transcript
            variant 2, mRNA.
ACCESSION   NM_001165880
VERSION     NM_001165880.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 1449)
  AUTHORS   Fitch,M.J., Campagnolo,L., Kuhnert,F. and Stuhlmann,H.
  TITLE     Egfl7, a novel epidermal growth factor-domain gene expressed in
            endothelial cells
  JOURNAL   Dev Dyn 230 (2), 316-324 (2004)
   PUBMED   15162510
REFERENCE   2  (bases 1 to 1449)
  AUTHORS   Hurt,P., Walter,L., Sudbrak,R., Klages,S., Muller,I., Shiina,T.,
            Inoko,H., Lehrach,H., Gunther,E., Reinhardt,R. and Himmelbauer,H.
  TITLE     The genomic sequence and comparative analysis of the rat major
            histocompatibility complex
  JOURNAL   Genome Res 14 (4), 631-639 (2004)
   PUBMED   15060004
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            JAXUCZ010000020.1.
            
            On May 27, 2021 this sequence version replaced NM_001165880.1.
            
            Transcript Variant: This variant (2) differs in the 3' coding
            region compared to variant 1. The resulting protein (isoform 2) has
            a shorter, distinct C-terminus compared to isoform 1.
            
            ##Evidence-Data-START##
            CDS exon combination :: BC127463.1, SRR22582416.1389293.1
                                    [ECO:0000331]
            RNAseq introns       :: single sample supports all introns
                                    SAMEA5760393, SAMEA5760434 [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-59                JAXUCZ010000020.1  4138053-4138111
            60-159              JAXUCZ010000020.1  4138226-4138325
            160-316             JAXUCZ010000020.1  4138694-4138850
            317-439             JAXUCZ010000020.1  4139026-4139148
            440-549             JAXUCZ010000020.1  4139250-4139359
            550-645             JAXUCZ010000020.1  4139452-4139547
            646-816             JAXUCZ010000020.1  4139670-4139840
            817-896             JAXUCZ010000020.1  4139922-4140001
            897-1449            JAXUCZ010000020.1  4140080-4140632
FEATURES             Location/Qualifiers
     source          1..1449
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="BN"
                     /db_xref="taxon:10116"
                     /chromosome="20"
                     /map="20p12"
     gene            1..1449
                     /gene="Egfl8"
                     /gene_synonym="Ng3"
                     /note="EGF-like-domain, multiple 8"
                     /db_xref="GeneID:406166"
                     /db_xref="RGD:1549706"
     exon            1..59
                     /gene="Egfl8"
                     /gene_synonym="Ng3"
                     /inference="alignment:Splign:2.1.0"
     exon            60..159
                     /gene="Egfl8"
                     /gene_synonym="Ng3"
                     /inference="alignment:Splign:2.1.0"
     exon            160..316
                     /gene="Egfl8"
                     /gene_synonym="Ng3"
                     /inference="alignment:Splign:2.1.0"
     CDS             219..1076
                     /gene="Egfl8"
                     /gene_synonym="Ng3"
                     /note="isoform 2 precursor is encoded by transcript
                     variant 2; EGF-like domain-containing protein 8; multiple
                     EGF-like domain protein 8; epidermal growth factor-like
                     protein 8; EGF-like protein 8; EGF-like domain 8"
                     /codon_start=1
                     /product="epidermal growth factor-like protein 8 isoform 2
                     precursor"
                     /protein_id="NP_001159352.1"
                     /db_xref="GeneID:406166"
                     /db_xref="RGD:1549706"
                     /translation="MEPWAGCCIFLRGFSFLLVLMTEGAKGGPFKESLGVCSKQTLLV
                     PLRYNESYSQPVYRPYLTLCAGRRICSTYRTTYRVAWREVRREVQQTHVVCCQGWKKP
                     HPGALTCEAICSKPCLNGGVCAGPDQCECASGWAGKHCHVDVDECRTSLTLCSHGCLN
                     TLGSFTCSCPNPLVLGPDGRACAGGPPESSTSAGILSVAVREADSEEHALRREVAELR
                     GRLERLEQWATQAGAWVRAVLPVAPEDLRPEQVAELWGRGDRIESLSDQVLLLEERLG
                     ACESPAPPP"
     exon            317..439
                     /gene="Egfl8"
                     /gene_synonym="Ng3"
                     /inference="alignment:Splign:2.1.0"
     exon            440..549
                     /gene="Egfl8"
                     /gene_synonym="Ng3"
                     /inference="alignment:Splign:2.1.0"
     exon            550..645
                     /gene="Egfl8"
                     /gene_synonym="Ng3"
                     /inference="alignment:Splign:2.1.0"
     exon            646..816
                     /gene="Egfl8"
                     /gene_synonym="Ng3"
                     /inference="alignment:Splign:2.1.0"
     exon            817..896
                     /gene="Egfl8"
                     /gene_synonym="Ng3"
                     /inference="alignment:Splign:2.1.0"
     exon            897..1449
                     /gene="Egfl8"
                     /gene_synonym="Ng3"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 atttggggac ctccaaagat tgtgtaggtc ctgctgaacg gcacttggct gaggagaaga
       61 gaggcccacc cgccagtgta gctgctgggg ctgctacagc tgctacggct gcagcctcca
      121 ggacagcctc caggacaagg gacaacaaca ggaggagaga ccaaagagaa gggaagagct
      181 cctgtaactg cttgtctgca gccctcagag tgaaagccat ggagccctgg gctgggtgct
      241 gcattttcct acgaggattc tcattcctcc tggtactgat gacagaaggg gccaaaggag
      301 gacctttcaa agaaagtttg ggagtgtgct ccaagcagac gctgctggtt ccactccgtt
      361 acaatgagtc ctacagtcaa ccagtgtaca gaccctacct gaccctgtgt gctggaaggc
      421 gcatatgtag cacctacagg accacgtacc gcgtggcctg gagggaagtg aggcgggagg
      481 tacagcagac acatgtggtg tgctgccagg gctggaagaa gccacaccca ggagctctca
      541 cctgtgaagc catctgctct aagccttgtc ttaatggagg tgtgtgcgcc ggaccggacc
      601 agtgcgagtg tgcctcaggc tgggcaggga agcactgcca cgtggatgtg gatgaatgca
      661 ggaccagcct taccctctgc tctcatggct gtctcaacac gctgggcagc ttcacctgca
      721 gctgtccgaa ccccctggtg ctggggcccg atggacgcgc ctgtgcaggg ggccctccgg
      781 agtcctcgac gagtgctggc atcctcagtg tggcagttcg ggaagcagac agcgaggagc
      841 atgccctgag gcgggaggtt gcagagcttc gtgggcgtct agagaggctg gagcagtggg
      901 ccacacaggc tggggcctgg gttcgagcag tgctgccggt ggcccctgaa gacctgcggc
      961 ctgaacaggt ggctgaactt tggggccggg gtgacaggat cgagtccctc agtgaccagg
     1021 tgctgctact ggaggaaagg ctgggtgcct gtgagtcccc cgctcctcct ccatgattga
     1081 agacccagcc tcaagtcagc actgcactcc tgaaaccctc cctcccacac tgtcctcgcc
     1141 ctgtcctggt ccccaggcca cttgtgtgtt gttgggcgac aggtctccag gccacagcca
     1201 ccttgccact ttgccattaa ccaggttcct gtgaggacaa cagcctgggc ccaggcctcc
     1261 atggatgaca aggaatgccg gcagcggctg gggtccctaa ctcacacaga aactatgcct
     1321 gctaacgtgg gatcccaaga tctggtacca accccgaggt gctacagcca cagagaaccc
     1381 cacgtctctc tttctcttta tttccagttg cttgctgtta tccagataat aaaaaccaac
     1441 cacacaaaa
//
