LOCUS       NM_001277341            1573 bp    mRNA    linear   ROD 31-MAR-2024
DEFINITION  Rattus norvegicus EGF containing fibulin extracellular matrix
            protein 2 (Efemp2), transcript variant 1, mRNA.
ACCESSION   NM_001277341
VERSION     NM_001277341.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 1573)
  AUTHORS   Liu,H. and Luo,J.
  TITLE     miR-211-5p contributes to chondrocyte differentiation by
            suppressing Fibulin-4 expression to play a role in osteoarthritis
  JOURNAL   J Biochem 166 (6), 495-502 (2019)
   PUBMED   31396630
REFERENCE   2  (bases 1 to 1573)
  AUTHORS   Zhang,D., Wang,S., Chen,J., Liu,H., Lu,J., Jiang,H., Huang,A. and
            Chen,Y.
  TITLE     Fibulin-4 promotes osteosarcoma invasion and metastasis by inducing
            epithelial to mesenchymal transition via the PI3K/Akt/mTOR pathway
  JOURNAL   Int J Oncol 50 (5), 1513-1530 (2017)
   PUBMED   28339091
REFERENCE   3  (bases 1 to 1573)
  AUTHORS   Wang,T., Wang,M., Fang,S., Wang,Q., Fang,R. and Chen,J.
  TITLE     Fibulin-4 is associated with prognosis of endometrial cancer
            patients and inhibits cancer cell invasion and metastasis via
            Wnt/beta-catenin signaling pathway
  JOURNAL   Oncotarget 8 (12), 18991-19012 (2017)
   PUBMED   28177909
REFERENCE   4  (bases 1 to 1573)
  AUTHORS   Li,R. and Wang,L.
  TITLE     Fibulin-4 is a novel Wnt/beta-Catenin pathway activator in human
            osteosarcoma
  JOURNAL   Biochem Biophys Res Commun 474 (4), 730-735 (2016)
   PUBMED   27157136
REFERENCE   5  (bases 1 to 1573)
  AUTHORS   Chen,J., Liu,Z., Fang,S., Fang,R., Liu,X., Zhao,Y., Li,X., Huang,L.
            and Zhang,J.
  TITLE     Fibulin-4 is associated with tumor progression and a poor prognosis
            in ovarian carcinomas
  JOURNAL   BMC Cancer 15, 91 (2015)
   PUBMED   25885889
  REMARK    Publication Status: Online-Only
REFERENCE   6  (bases 1 to 1573)
  AUTHORS   Huawei,P., Qian,C., Chuan,T., Lei,L., Laing,W., Wenlong,X. and
            Wenzhi,L.
  TITLE     Decreased expression of fibulin-4 in aortic wall of aortic
            dissection
  JOURNAL   Vascular 22 (1), 35-41 (2014)
   PUBMED   23518852
REFERENCE   7  (bases 1 to 1573)
  AUTHORS   Kappanayil,M., Nampoothiri,S., Kannan,R., Renard,M., Coucke,P.,
            Malfait,F., Menon,S., Ravindran,H.K., Kurup,R., Faiyaz-Ul-Haque,M.,
            Kumar,K. and De Paepe,A.
  TITLE     Characterization of a distinct lethal arteriopathy syndrome in
            twenty-two infants associated with an identical, novel mutation in
            FBLN4 gene, confirms fibulin-4 as a critical determinant of human
            vascular elastogenesis
  JOURNAL   Orphanet J Rare Dis 7, 61 (2012)
   PUBMED   22943132
  REMARK    Publication Status: Online-Only
REFERENCE   8  (bases 1 to 1573)
  AUTHORS   Al-Hassnan,Z.N., Almesned,A.R., Tulbah,S., Hakami,A., Al-Omrani,A.,
            Al Sehly,A., Mohammed,S., Majid,S., Meyer,B. and Al-Fayyadh,M.
  TITLE     Recessively inherited severe aortic aneurysm caused by mutated
            EFEMP2
  JOURNAL   Am J Cardiol 109 (11), 1677-1680 (2012)
   PUBMED   22440127
REFERENCE   9  (bases 1 to 1573)
  AUTHORS   Horiguchi,M., Inoue,T., Ohbayashi,T., Hirai,M., Noda,K.,
            Marmorstein,L.Y., Yabe,D., Takagi,K., Akama,T.O., Kita,T.,
            Kimura,T. and Nakamura,T.
  TITLE     Fibulin-4 conducts proper elastogenesis via interaction with
            cross-linking enzyme lysyl oxidase
  JOURNAL   Proc Natl Acad Sci U S A 106 (45), 19029-19034 (2009)
   PUBMED   19855011
REFERENCE   10 (bases 1 to 1573)
  AUTHORS   Gonzalez-Begne,M., Lu,B., Han,X., Hagen,F.K., Hand,A.R.,
            Melvin,J.E. and Yates,J.R.
  TITLE     Proteomic analysis of human parotid gland exosomes by
            multidimensional protein identification technology (MudPIT)
  JOURNAL   J Proteome Res 8 (3), 1304-1314 (2009)
   PUBMED   19199708
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            CB811907.1, FM037652.1 and JAXUCZ010000001.1.
            
            Transcript Variant: This variant (1) represents the longer
            transcript and encodes the longer isoform (1).
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR22399481.2967150.1,
                                           SRR26643289.3575.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA5760383, SAMEA5760386
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-38                CB811907.1         1-38
            39-565              FM037652.1         1-527
            566-594             JAXUCZ010000001.1  212214668-212214696
            595-711             JAXUCZ010000001.1  212214937-212215053
            712-831             JAXUCZ010000001.1  212215165-212215284
            832-951             JAXUCZ010000001.1  212216959-212217078
            952-1078            JAXUCZ010000001.1  212217163-212217289
            1079-1274           JAXUCZ010000001.1  212217477-212217672
            1275-1573           JAXUCZ010000001.1  212218441-212218739
FEATURES             Location/Qualifiers
     source          1..1573
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /db_xref="taxon:10116"
                     /chromosome="1"
                     /map="1q43"
     gene            1..1573
                     /gene="Efemp2"
                     /note="EGF containing fibulin extracellular matrix protein
                     2"
                     /db_xref="GeneID:293677"
                     /db_xref="RGD:1359496"
     exon            1..97
                     /gene="Efemp2"
                     /inference="alignment:Splign:2.1.0"
     exon            98..215
                     /gene="Efemp2"
                     /inference="alignment:Splign:2.1.0"
     CDS             105..1436
                     /gene="Efemp2"
                     /note="isoform 1 precursor is encoded by transcript
                     variant 1; EGF-containing fibulin-like extracellular
                     matrix protein 2"
                     /codon_start=1
                     /product="EGF-containing fibulin-like extracellular matrix
                     protein 2 isoform 1 precursor"
                     /protein_id="NP_001264270.1"
                     /db_xref="GeneID:293677"
                     /db_xref="RGD:1359496"
                     /translation="MLPFASCLPGSLLLWAFLLLLLGAASPQDSEEPDSYTECTDGYE
                     WDADSQHCRDVNECLTIPEACKGEMKCINHYGGYLCLPRSAAVISDLHSEGPPPPAAS
                     AQHPNPCPPGYEPDEQESCVDVDECTQAIHDCRPSQDCHNLPGSYQCTCPDGYRKIGP
                     ECVDIDECRYRYCQHRCVNLPGSFRCQCEPGFQLGPNNRSCVDVNECDMGAPCEQRCF
                     NSYGTFLCRCNQGYELHRDGFSCSDIDECSYSSYLCQYRCVNEPGRFSCHCPQGYQLL
                     ATRLCQDIDECETGAHQCSEAQTCVNFHGGYRCVDTNRCVEPYVQVSDNRCLCPASNP
                     LCREQPSSIVHRYMSITSERSVPADVFQIQATSVYPGAYNAFQIRAGNTQGDFYIRQI
                     NNVSAMLVLARPVTGPREYVLDLEMVTMNSLMSYRASSVLRLTVFVGAYTF"
     exon            216..264
                     /gene="Efemp2"
                     /inference="alignment:Splign:2.1.0"
     exon            265..471
                     /gene="Efemp2"
                     /inference="alignment:Splign:2.1.0"
     exon            472..594
                     /gene="Efemp2"
                     /inference="alignment:Splign:2.1.0"
     exon            595..711
                     /gene="Efemp2"
                     /inference="alignment:Splign:2.1.0"
     exon            712..831
                     /gene="Efemp2"
                     /inference="alignment:Splign:2.1.0"
     exon            832..951
                     /gene="Efemp2"
                     /inference="alignment:Splign:2.1.0"
     exon            952..1078
                     /gene="Efemp2"
                     /inference="alignment:Splign:2.1.0"
     exon            1079..1274
                     /gene="Efemp2"
                     /inference="alignment:Splign:2.1.0"
     exon            1275..1573
                     /gene="Efemp2"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 ggccgcggcg ggcgggcggg gggcgcttcc tggggccgcg cgtccagtga gctgcgcggt
       61 ccgtccgtcc gcccgcaggc actgctggag tcaacgcccc caggatgctc ccatttgcct
      121 cctgcctccc cgggtcttta ctgctctggg cgtttctgct gttgctcttg ggagcagcgt
      181 ccccacagga ttccgaagag ccggacagct acacggaatg cacagatggc tatgagtggg
      241 atgcagacag ccagcattgc cgggatgtca atgagtgcct gaccatcccc gaggcttgca
      301 agggtgaaat gaaatgcatc aaccactacg ggggctattt gtgtctgcct cgctctgctg
      361 ccgtcatcag tgatctccac agtgaaggac ctccaccacc agcagcctct gctcaacacc
      421 caaatccttg ccctccgggc tatgaacctg atgaacagga gagctgtgtg gatgtggatg
      481 agtgtaccca ggctatacat gactgtcgcc ctagccagga ctgccataac cttcctggct
      541 cctatcagtg cacctgcccc gatggttacc gaaaaattgg gcccgaatgt gtagacatag
      601 atgagtgtcg ctaccgctac tgccagcacc gatgcgtgaa cctgccaggc tctttccgat
      661 gccagtgtga gcccggcttc cagttgggac ctaacaaccg ctcttgtgtg gatgtgaacg
      721 agtgtgacat gggagccccg tgtgagcagc gctgcttcaa ctcctatggg accttcctgt
      781 gtcgctgtaa ccagggctat gagctacacc gggatggctt ctcctgcagt gatatcgatg
      841 agtgcagcta ctccagttac ctctgccagt accgctgtgt caatgagcca ggtcgcttct
      901 cctgtcactg cccacaaggc taccaactgc tggccacaag gctctgccaa gacattgacg
      961 agtgcgaaac aggtgcacac cagtgttcgg aggcccaaac ctgcgtcaac ttccatgggg
     1021 gttaccgctg tgtggacacc aaccgttgtg tggagcccta tgtccaagtg tcggacaacc
     1081 gctgcctctg tcctgcctcc aacccccttt gtcgagagca accgtcatcc atcgtgcacc
     1141 gctacatgag catcacctca gagcgaagtg tgcctgccga cgtgtttcag atccaggcaa
     1201 cctctgtcta ccctggcgcc tacaatgcct ttcagatccg tgctgggaac acacaggggg
     1261 acttctacat taggcaaatc aacaatgtca gcgccatgct ggtccttgcc agaccggtga
     1321 caggaccccg ggagtacgtc ctggacctcg agatggtcac catgaactcc cttatgagtt
     1381 accgggccag ctctgtactg agactcaccg tctttgtggg agcctatacc ttctgaagaa
     1441 gaccctcagg gaagggtcat gttggggggc cccttccccc ctcccatagc ttaagaagcc
     1501 ctgggggctg aaggatgact gttctgttta aagggactat gatgtgaagg acaataaagg
     1561 gagaaagaag gag
//
