LOCUS       NM_001398914            2137 bp    mRNA    linear   ROD 31-MAR-2024
DEFINITION  Rattus norvegicus eukaryotic elongation factor,
            selenocysteine-tRNA-specific (Eefsec), transcript variant 1, mRNA.
ACCESSION   NM_001398914 XM_006236864
VERSION     NM_001398914.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 2137)
  AUTHORS   Gonzalez-Flores,J.N., Gupta,N., DeMong,L.W. and Copeland,P.R.
  TITLE     The selenocysteine-specific elongation factor contains a novel and
            multi-functional domain
  JOURNAL   J Biol Chem 287 (46), 38936-38945 (2012)
   PUBMED   22992746
  REMARK    GeneRIF: SBP2/SECIS complex activates eEFSec by directing
            functional interactions between Domain IV and the ribosome to
            promote Sec-tRNA(Sec) binding and accommodation into the ribosomal
            A-site
REFERENCE   2  (bases 1 to 2137)
  AUTHORS   Small-Howard,A., Morozova,N., Stoytcheva,Z., Forry,E.P.,
            Mansell,J.B., Harney,J.W., Carlson,B.A., Xu,X.M., Hatfield,D.L. and
            Berry,M.J.
  TITLE     Supramolecular complexes mediate selenocysteine incorporation in
            vivo
  JOURNAL   Mol Cell Biol 26 (6), 2337-2346 (2006)
   PUBMED   16508009
REFERENCE   3  (bases 1 to 2137)
  AUTHORS   Zhang,S., Lloyd,R., Bowden,G., Glickman,B.W. and de Boer,J.G.
  TITLE     Thymic lymphomas arising in Msh2 deficient mice display a large
            increase in mutation frequency and an altered mutational spectrum
  JOURNAL   Mutat Res 500 (1-2), 67-74 (2002)
   PUBMED   11890935
REFERENCE   4  (bases 1 to 2137)
  AUTHORS   Smits,R., Hofland,N., Edelmann,W., Geugien,M., Jagmohan-Changur,S.,
            Albuquerque,C., Breukel,C., Kucherlapati,R., Kielman,M.F. and
            Fodde,R.
  TITLE     Somatic Apc mutations are selected upon their capacity to
            inactivate the beta-catenin downregulating activity
  JOURNAL   Genes Chromosomes Cancer 29 (3), 229-239 (2000)
   PUBMED   10992298
REFERENCE   5  (bases 1 to 2137)
  AUTHORS   Fagegaltier,D., Hubert,N., Yamada,K., Mizutani,T., Carbon,P. and
            Krol,A.
  TITLE     Characterization of mSelB, a novel mammalian elongation factor for
            selenoprotein translation
  JOURNAL   EMBO J 19 (17), 4796-4805 (2000)
   PUBMED   10970870
REFERENCE   6  (bases 1 to 2137)
  AUTHORS   Tujebajeva,R.M., Copeland,P.R., Xu,X.M., Carlson,B.A., Harney,J.W.,
            Driscoll,D.M., Hatfield,D.L. and Berry,M.J.
  TITLE     Decoding apparatus for eukaryotic selenocysteine insertion
  JOURNAL   EMBO Rep 1 (2), 158-163 (2000)
   PUBMED   11265756
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            JAXUCZ010000004.1.
            
            On Dec 28, 2021 this sequence version replaced XM_006236864.4.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC168694.1, SRR26643429.17207.1
                                           [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA5760383,
                                           SAMEA5760386 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-315               JAXUCZ010000004.1  122472733-122473047 c
            316-523             JAXUCZ010000004.1  122397603-122397810 c
            524-620             JAXUCZ010000004.1  122381171-122381267 c
            621-785             JAXUCZ010000004.1  122378254-122378418 c
            786-1445            JAXUCZ010000004.1  122315375-122316034 c
            1446-1602           JAXUCZ010000004.1  122301925-122302081 c
            1603-2137           JAXUCZ010000004.1  122277433-122277967 c
FEATURES             Location/Qualifiers
     source          1..2137
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="BN"
                     /db_xref="taxon:10116"
                     /chromosome="4"
                     /map="4q34"
     gene            1..2137
                     /gene="Eefsec"
                     /gene_synonym="RGD1560552"
                     /note="eukaryotic elongation factor,
                     selenocysteine-tRNA-specific"
                     /db_xref="GeneID:500255"
                     /db_xref="RGD:1560552"
     exon            1..315
                     /gene="Eefsec"
                     /gene_synonym="RGD1560552"
                     /inference="alignment:Splign:2.1.0"
     CDS             42..1793
                     /gene="Eefsec"
                     /gene_synonym="RGD1560552"
                     /note="isoform 1 is encoded by transcript variant 1;
                     selenocysteine-specific elongation factor"
                     /codon_start=1
                     /product="selenocysteine-specific elongation factor
                     isoform 1"
                     /protein_id="NP_001385843.1"
                     /db_xref="GeneID:500255"
                     /db_xref="RGD:1560552"
                     /translation="MAGRRVNVNVGVLGHIDSGKTALARALSTTASTAAFDKQPQSRE
                     RGITLDLGFSCFVVPLPGAEPGTSDTLLQVTLVDCPGHASLIRTIIGGAQIIDLMMLV
                     IDVTKGMQTQSAECLVIGQIACQKLVVVLNKIDLLAEGKRQAAIDKMTKKMQKTLENT
                     KFRGAPIIPVAAKPGGPEAPETEAPQGISELIELLKSQISIPTRDPSGPFLMSVDHCF
                     SIKGQGTVMTGTILSGTISLGDSVEIPALKVVKKVKSMQMFHTPVTSAMQGDRLGICV
                     TQFDPKLLERGLVCAPESLHTVHAALISVEKIPYFRGPLQTKAKFHITVGHETVMGRM
                     LFFSPAPDNFDSEPVLDSFDLSREYLFQEQYLCKDSMPTATEGNDEADTKAGNSPEGH
                     CPRQQWALVEFEKPVTCPRLCLVIGSRLDADIHTNTCRLAFHGVLVQGLEDKNYTESF
                     LPALRVYKLKHKHGLVERVMDDYSVIGRSLFKKETNIQLFVGLKVQLSTGEQGIIDSA
                     FGQSGKFKIHIPGGLSPESKKILTPTLKKRSRAGRGETTKQEESTERPEPIQPVTLSL
                     SFKRYVFDTQKRMVQTP"
     exon            316..523
                     /gene="Eefsec"
                     /gene_synonym="RGD1560552"
                     /inference="alignment:Splign:2.1.0"
     exon            524..620
                     /gene="Eefsec"
                     /gene_synonym="RGD1560552"
                     /inference="alignment:Splign:2.1.0"
     exon            621..785
                     /gene="Eefsec"
                     /gene_synonym="RGD1560552"
                     /inference="alignment:Splign:2.1.0"
     exon            786..1445
                     /gene="Eefsec"
                     /gene_synonym="RGD1560552"
                     /inference="alignment:Splign:2.1.0"
     exon            1446..1602
                     /gene="Eefsec"
                     /gene_synonym="RGD1560552"
                     /inference="alignment:Splign:2.1.0"
     exon            1603..2137
                     /gene="Eefsec"
                     /gene_synonym="RGD1560552"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 ggggccggcg attaggcgac cgcgtgagtg gcggcgacgg gatggcgggc cggcgggtaa
       61 atgtgaatgt gggtgtgctg ggtcatatcg atagcggcaa gacagcgctg gcgcgcgcgc
      121 tgagcactac ggcctccacc gctgccttcg acaagcagcc gcagagccgc gagcgcggca
      181 taacgctcga cctgggcttc tcctgttttg tggtgccgct gccgggggct gaacccggga
      241 ctagcgacac gctgctgcag gtcacgctgg tcgactgtcc cgggcacgcc tccctcatcc
      301 ggaccatcat tggcggtgcc cagatcattg atttgatgat gctcgtgatt gatgtgacca
      361 agggaatgca gacacagtcc gcagagtgcc ttgtgatcgg ccagattgcc tgccagaagc
      421 tggttgtggt gctgaacaaa atagacctgt tagcagaagg gaagagacag gcagcgattg
      481 acaaaatgac caagaaaatg cagaagactc ttgagaacac caaattccgg ggtgcaccga
      541 ttattcccgt ggcagccaag cctgggggac cagaggcccc tgaaacagaa gctccacagg
      601 gcatctcaga actcattgag cttctgaagt cccagatttc catcccaacg agagacccct
      661 ctggaccatt ccttatgtct gtggatcact gcttctccat caagggtcag ggcaccgtga
      721 tgactgggac catcctctcg ggcactatca gcctcgggga cagtgtggag atccctgccc
      781 tcaaggtggt gaagaaagtg aagtccatgc agatgttcca tacacctgtc acctctgcca
      841 tgcaaggaga ccgactaggc atctgtgtta cccagtttga ccccaagctg ttggagcgtg
      901 gtctggtatg tgcaccagag tctctgcaca ccgttcatgc agccctcatc tctgtggaaa
      961 agattcctta cttccggggg cccctgcaga cgaaggccaa gttccacatc acagtgggcc
     1021 atgagacagt catgggccgt atgctgttct tcagccctgc tcctgacaat tttgactcag
     1081 aacccgtgct agattccttc gacctttccc gagaatacct cttccaagag cagtacctgt
     1141 gcaaggattc catgcccaca gccacagaag gtaatgatga ggcagacaca aaagcaggca
     1201 actccccaga gggccactgt ccccggcagc aatgggccct ggtagagttt gagaagccag
     1261 tcacctgccc ccgactctgc ctggtgattg gctccagact ggatgctgac attcacacca
     1321 acacatgccg gctggccttc catggtgtct tggtacaagg actggaggac aagaactaca
     1381 cagagagctt cctgccagca ctgagggttt acaagctgaa gcacaagcat ggccttgtgg
     1441 agcgggtaat ggatgactac agcgtgattg gacgttccct gttcaaaaag gagaccaaca
     1501 tccagctctt tgtggggctt aaggtacagc tgtccacagg agagcagggc atcatcgaca
     1561 gtgccttcgg ccaaagcggc aagttcaaga tccacatccc tggtggcctc agccctgagt
     1621 ctaagaagat cctgacacct acactcaaga agcggagccg agctggccgt ggggaaacca
     1681 ccaagcaaga agagagcact gagcgtccag aacccataca gcctgtgaca ctcagcctgt
     1741 ctttcaagcg ctatgtcttt gacacccaga agcgcatggt acagactccc tgaatgaacc
     1801 gtcctgtcca gctacaagct gtaccacagt gccagatgcc cccagacgtg cctctacctc
     1861 ctcagtccta taggcagtca gccccatcca actgccatcc gcactgttac tgagccccat
     1921 tgagaaaacg tgggggaagt gaagagcagt gccaggaggg cctgtcactc catccctgca
     1981 tccacccagg atagcaacta ggcccattag gaaagcgtcc tagcggagac aagaacatcg
     2041 gtcttcccca gttagggaat gtgcagccac tcagccctgc cccaagtagc tggcaccctc
     2101 caactagaaa taaacatccc ctatgtggcc tagtgta
//
