LOCUS       NM_017245               2944 bp    mRNA    linear   ROD 03-FEB-2024
DEFINITION  Rattus norvegicus eukaryotic translation elongation factor 2
            (Eef2), mRNA.
ACCESSION   NM_017245
VERSION     NM_017245.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 2944)
  AUTHORS   Vasamsetti,B.M.K., Liu,Z., Park,Y.S. and Cho,N.J.
  TITLE     Muscarinic acetylcholine receptors regulate the dephosphorylation
            of eukaryotic translation elongation factor 2 in SNU-407 colon
            cancer cells
  JOURNAL   Biochem Biophys Res Commun 516 (2), 424-429 (2019)
   PUBMED   31227218
REFERENCE   2  (bases 1 to 2944)
  AUTHORS   Martinetz,S., Meinung,C.P., Jurek,B., von Schack,D., van den
            Burg,E.H., Slattery,D.A. and Neumann,I.D.
  TITLE     De Novo Protein Synthesis Mediated by the Eukaryotic Elongation
            Factor 2 Is Required for the Anxiolytic Effect of Oxytocin
  JOURNAL   Biol Psychiatry 85 (10), 802-811 (2019)
   PUBMED   30826070
  REMARK    GeneRIF: Results show that oxytocin (OXT) recruits several
            intracellular signaling cascades to induce protein synthesis, which
            mediates the anxiolytic effects of OXT within the paraventricular
            nucleus of the hypothalamus and suggests that eukaryotic elongation
            factor 2 represents a novel target for anxiety-related disorders.
REFERENCE   3  (bases 1 to 2944)
  AUTHORS   Yang,Q., Guo,X.P., Cheng,Y.L. and Wang,Y.
  TITLE     MicroRNA-143-5p targeting eEF2 gene mediates intervertebral disc
            degeneration through the AMPK signaling pathway
  JOURNAL   Arthritis Res Ther 21 (1), 97 (2019)
   PUBMED   30987676
  REMARK    GeneRIF: A reduction in eEF2, an increase in miR-143-5p, and
            activation of AMPK signaling was observed in degenerative disc
            degeneration.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 2944)
  AUTHORS   Gu,H., Chen,J., Song,Y. and Shao,H.
  TITLE     Gastric Adenocarcinoma Predictive Long Intergenic Non-Coding RNA
            Promotes Tumor Occurrence and Progression in Non-Small Cell Lung
            Cancer via Regulation of the miR-661/eEF2K Signaling Pathway
  JOURNAL   Cell Physiol Biochem 51 (5), 2136-2147 (2018)
   PUBMED   30522114
REFERENCE   5  (bases 1 to 2944)
  AUTHORS   Lomonosova,Y.N., Belova,S.P., Mirzoev,T.M., Kozlovskaya,I.B. and
            Shenkman,B.S.
  TITLE     Eukaryotic elongation factor 2 kinase activation in M. soleus under
            14-day hindlimb unloading of rats
  JOURNAL   Dokl Biochem Biophys 474 (1), 165-167 (2017)
   PUBMED   28726101
  REMARK    GeneRIF: Eukaryotic elongation factor 2 kinase activation in M.
            soleus under 14-day hindlimb unloading of rats
REFERENCE   6  (bases 1 to 2944)
  AUTHORS   Johnson,G., Gotlib,J., Haroutunian,V., Bierer,L., Nairn,A.C.,
            Merril,C. and Wallace,W.
  TITLE     Increased phosphorylation of elongation factor 2 in Alzheimer's
            disease
  JOURNAL   Brain Res Mol Brain Res 15 (3-4), 319-326 (1992)
   PUBMED   1331687
REFERENCE   7  (bases 1 to 2944)
  AUTHORS   Carlberg,U., Nilsson,A. and Nygard,O.
  TITLE     Functional properties of phosphorylated elongation factor 2
  JOURNAL   Eur J Biochem 191 (3), 639-645 (1990)
   PUBMED   2390990
REFERENCE   8  (bases 1 to 2944)
  AUTHORS   Oleinikov,A.V., Jokhadze,G.G. and Alakhov,Yu.B.
  TITLE     Primary structure of rat liver elongation factor 2 deduced from the
            cDNA sequence
  JOURNAL   FEBS Lett 248 (1-2), 131-136 (1989)
   PUBMED   2721670
REFERENCE   9  (bases 1 to 2944)
  AUTHORS   Nairn,A.C. and Palfrey,H.C.
  TITLE     Identification of the major Mr 100,000 substrate for
            calmodulin-dependent protein kinase III in mammalian cells as
            elongation factor-2
  JOURNAL   J Biol Chem 262 (36), 17299-17303 (1987)
   PUBMED   3693353
REFERENCE   10 (bases 1 to 2944)
  AUTHORS   Kohno,K., Uchida,T., Ohkubo,H., Nakanishi,S., Nakanishi,T.,
            Fukui,T., Ohtsuka,E., Ikehara,M. and Okada,Y.
  TITLE     Amino acid sequence of mammalian elongation factor 2 deduced from
            the cDNA sequence: homology with GTP-binding proteins
  JOURNAL   Proc Natl Acad Sci U S A 83 (14), 4978-4982 (1986)
   PUBMED   3014523
COMMENT     PROVISIONAL REFSEQ: This record has not yet been subject to final
            NCBI review. The reference sequence was derived from BC066661.1.
            
            [WARNING] On Mar 18, 2024 this sequence was replaced by
            NM_017245.3.
            
            On Mar 14, 2004 this sequence version replaced NM_017245.1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC066661.1, Y07504.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA5756307, SAMEA5760383
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
FEATURES             Location/Qualifiers
     source          1..2944
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /db_xref="taxon:10116"
                     /chromosome="7"
                     /map="7q11"
     gene            1..2944
                     /gene="Eef2"
                     /gene_synonym="Ef-2"
                     /note="eukaryotic translation elongation factor 2"
                     /db_xref="GeneID:29565"
                     /db_xref="RGD:61979"
     exon            1..66
                     /gene="Eef2"
                     /gene_synonym="Ef-2"
                     /inference="alignment:Splign:2.1.0"
     CDS             64..2640
                     /gene="Eef2"
                     /gene_synonym="Ef-2"
                     /codon_start=1
                     /product="elongation factor 2"
                     /protein_id="NP_058941.1"
                     /db_xref="GeneID:29565"
                     /db_xref="RGD:61979"
                     /translation="MVNFTVDQIRAIMDKKANIRNMSVIAHVDHGKSTLTDSLVCKAG
                     IIASARAGETRFTDTRKDEQERCITIKSTAISLFYELSENDLNFIKQSKDGSGFLINL
                     IDSPGHVDFSSEVTAALRVTDGALVVVDCVSGVCVQTETVLRQAIAERIKPVLMMNKM
                     DRALLELQLEPEELYQTFQRIVENVNVIISTYGEGESGPMGNIMIDPVLGTVGFGSGL
                     HGWAFTLKQFAEMYVAKFAAKGEGQLGAAERAKKVEDMMKKLWGDRYFDPANGKFSKS
                     ANSPDGKKLPRTFCQLILDPIFKVFDAIMNFRKEETAKLIEKLDIKLDSEDKDKEGKP
                     LLKAVMRRWLPAGDALLQMITIHLPSPVTAQKYRCELLYEGPPDDEAAMGIKSCDPKG
                     PLMMYISKMVPTSDKGRFYAFGRVFSGVVSTGLKVRIMGPNYTPGKKEDLYLKPIQRT
                     ILMMGRYVEPIEDVPCGNIVGLVGVDQFLVKTGTITTFEHAHNMRVMKFSVSPVVRVA
                     VEAKNPADLPKLVEGLKRLAKSDPMVQCIIEESGEHIIAGAGELHLEICLKDLEEDHA
                     CIPIKKSDPVVSYRETVSEESNVLCLSKSPNKHNRLYMKARPFPDGLAEDIDKGEVSA
                     RQELKARARYLAEKYEWDVAEARKIWCFGPDGTGPNILTDITKGVQYLNEIKDSVVAG
                     FQWATKEGALCEENMRGVRFDVHDVTLHADAIHRGGGQIIPTARRCLYASVLTAQPRL
                     MEPIYLVEIQCPEQVVGGIYGVLNRKRGHVFEESQVAGTPMFVVKAYLPVNESFGFTA
                     DLRSNTGGQAFPQCVFDHWQILPGDPFDNSSRPSQVVAETRKRKGLKEGIPALDNFLD
                     KL"
     exon            67..281
                     /gene="Eef2"
                     /gene_synonym="Ef-2"
                     /inference="alignment:Splign:2.1.0"
     exon            282..463
                     /gene="Eef2"
                     /gene_synonym="Ef-2"
                     /inference="alignment:Splign:2.1.0"
     exon            464..675
                     /gene="Eef2"
                     /gene_synonym="Ef-2"
                     /inference="alignment:Splign:2.1.0"
     exon            676..854
                     /gene="Eef2"
                     /gene_synonym="Ef-2"
                     /inference="alignment:Splign:2.1.0"
     exon            855..1213
                     /gene="Eef2"
                     /gene_synonym="Ef-2"
                     /inference="alignment:Splign:2.1.0"
     exon            1214..1409
                     /gene="Eef2"
                     /gene_synonym="Ef-2"
                     /inference="alignment:Splign:2.1.0"
     exon            1410..1668
                     /gene="Eef2"
                     /gene_synonym="Ef-2"
                     /inference="alignment:Splign:2.1.0"
     exon            1669..2130
                     /gene="Eef2"
                     /gene_synonym="Ef-2"
                     /inference="alignment:Splign:2.1.0"
     exon            2131..2446
                     /gene="Eef2"
                     /gene_synonym="Ef-2"
                     /inference="alignment:Splign:2.1.0"
     exon            2447..2917
                     /gene="Eef2"
                     /gene_synonym="Ef-2"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 cgccatcgtc ggcgcgcttc cctgttcacc tctgtatttg agaatccgac gccatctgcc
       61 accatggtga acttcacagt agatcagatc cgtgccatca tggacaagaa agccaacatc
      121 cggaacatgt cagtcatcgc tcacgtggac cacggcaagt ccacgttgac cgactccctt
      181 gtgtgcaagg ctggcatcat cgcctctgcc cgagccgggg agacacgctt cactgacact
      241 cgaaaggatg agcaggagcg ctgcatcacc atcaagtcca ctgccatctc cctcttctat
      301 gagctctccg agaacgacct gaacttcatt aagcagagca aggatggctc gggcttcctc
      361 atcaacctca ttgactctcc aggtcatgtg gacttctcct cagaggtgac cgctgctctg
      421 cgtgtcactg atggagcact ggtggtggtg gactgtgtgt ctggtgtctg tgtgcagaca
      481 gagacagtgc tgcggcaggc catcgctgag cgcatcaagc ccgtgctgat gatgaacaag
      541 atggaccggg ccctgctgga actgcaactg gagcctgagg agctctacca gaccttccag
      601 cgcatcgtgg agaacgtcaa tgtcatcatc tccacctatg gcgagggcga gagtggaccc
      661 atgggcaata tcatgatcga tcctgtcctg ggcactgtag gctttggctc tggcctgcat
      721 gggtgggcct tcacactgaa gcagtttgca gagatgtatg tggccaagtt tgcagccaag
      781 ggtgagggcc agctgggtgc agctgagcgg gccaagaagg tagaagacat gatgaagaag
      841 ctgtggggag accggtactt tgacccggcc aacggcaagt tcagcaagtc agccaatagc
      901 ccagacggga agaaacttcc ccgcaccttc tgccagctca tcttggaccc catcttcaag
      961 gtgtttgatg ccatcatgaa cttcaggaag gaggagacag ccaagctgat cgagaagctg
     1021 gacatcaagc tggacagtga ggacaaggac aaggagggca aaccactgct gaaggctgtg
     1081 atgcgtcgct ggctgcctgc tggtgacgcc ctgctacaga tgatcaccat ccacttgccg
     1141 tccccggtca ctgcacagaa gtaccgttgt gagctgctgt acgagggccc acctgatgac
     1201 gaggccgcca tgggtattaa gagctgcgac cccaaaggcc ccctaatgat gtacatctcc
     1261 aagatggtgc caacatctga caaaggccgc ttctatgcct tcggtagagt gttctctggg
     1321 gtggtgtcca caggtctgaa ggtccggatc atgggcccca actatacacc tgggaagaag
     1381 gaggacctgt acctgaagcc tatccagagg accattctga tgatgggccg ctatgtggaa
     1441 cccattgagg atgtgccctg tggcaacatt gtggggttgg tcggggtgga ccagttcctt
     1501 gtgaagaccg gcaccatcac tacctttgag cacgctcaca acatgcgggt gatgaagttc
     1561 agcgtcagcc ctgtcgtcag ggtggcagtg gaggccaaga acccagctga cctgcccaag
     1621 ctggtggagg ggctgaagcg gctggctaag tctgacccta tggtgcagtg catcatcgag
     1681 gagtctgggg agcacatcat tgctggtgct ggagagctgc acctggagat ctgccttaag
     1741 gacctggagg aggaccatgc ctgcatcccc atcaagaaat ctgaccctgt cgtgtcctac
     1801 cgggagacag tcagtgagga gtccaacgtg ctctgtcttt ccaagtcccc aaacaagcac
     1861 aacaggctgt acatgaaggc caggcccttc cctgatggcc tagcagagga catcgataag
     1921 ggtgaggtat ctgcccgcca ggagctcaag gcgcgtgccc gttacctggc cgaaaagtat
     1981 gagtgggatg ttgctgaagc ccgcaagatc tggtgctttg gacctgatgg cactggtccc
     2041 aacattctca ccgacatcac caagggtgtg cagtacctga atgagatcaa ggacagtgtg
     2101 gtggctggat tccagtgggc cactaaggag ggtgctcttt gtgaggagaa catgcgtggt
     2161 gtgcgatttg atgtccatga tgtgaccctg catgctgatg ccatccaccg aggaggtggt
     2221 cagatcatcc ccacagcgcg ccgctgcctc tatgccagtg tgctgactgc acagccccgc
     2281 ctcatggagc ctatctacct ggtggagatt cagtgtcctg agcaagtggt gggcggaatc
     2341 tatggtgtcc tgaacaggaa gcgtggccat gtctttgagg agtcccaggt agctgggacc
     2401 cccatgttcg tggtcaaggc ctacctgcct gtcaatgaat cctttggctt cactgccgac
     2461 cttcgatcca acactggtgg ccaggccttc ccccagtgtg tgtttgacca ctggcagatc
     2521 ctgcctgggg acccctttga caacagcagc cgccccagcc aagttgtagc tgagacccgc
     2581 aagcgcaaag gcctgaagga gggcatccca gcgctggaca acttcctgga caaactgtag
     2641 gcagcctggt actgccacac gctgcacagt gcccgcccat cagaagacac cttgagactg
     2701 tccccacagt gcccctctgg aggctgctgg ggccatcctg acatcactca gcactcactt
     2761 gctaccaatt ctatttattt cggaattaca aggcagcgtg aatggccctc tgcaggctgg
     2821 actggcaggc cgtggggtgg gcagcacacg gtggttagca ttttcagagg gaaataacgc
     2881 agatgtccaa aattctaaat aaatgcattc agaggttaaa aaaaaaaaaa aaaaaaaaaa
     2941 aaaa
//
