LOCUS       NM_078623               1560 bp    mRNA    linear   ROD 03-APR-2024
DEFINITION  Rattus norvegicus enoyl-CoA hydratase, short chain 1 (Echs1), mRNA;
            nuclear gene for mitochondrial product.
ACCESSION   NM_078623
VERSION     NM_078623.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 1560)
  AUTHORS   Yamada,K., Aiba,K., Kitaura,Y., Kondo,Y., Nomura,N., Nakamura,Y.,
            Fukushi,D., Murayama,K., Shimomura,Y., Pitt,J., Yamaguchi,S.,
            Yokochi,K. and Wakamatsu,N.
  TITLE     Clinical, biochemical and metabolic characterisation of a mild form
            of human short-chain enoyl-CoA hydratase deficiency: significance
            of increased N-acetyl-S-(2-carboxypropyl)cysteine excretion
  JOURNAL   J Med Genet 52 (10), 691-698 (2015)
   PUBMED   26251176
REFERENCE   2  (bases 1 to 1560)
  AUTHORS   Zhang,X., Yang,J., Guo,Y., Ye,H., Yu,C., Xu,C., Xu,L., Wu,S.,
            Sun,W., Wei,H., Gao,X., Zhu,Y., Qian,X., Jiang,Y., Li,Y. and He,F.
  TITLE     Functional proteomic analysis of nonalcoholic fatty liver disease
            in rat models: enoyl-coenzyme a hydratase down-regulation
            exacerbates hepatic steatosis
  JOURNAL   Hepatology 51 (4), 1190-1199 (2010)
   PUBMED   20162621
  REMARK    GeneRIF: enoyl-coenzyme a hydratase down-regulation exacerbates
            hepatic steatosis.
REFERENCE   3  (bases 1 to 1560)
  AUTHORS   Mootha,V.K., Bunkenborg,J., Olsen,J.V., Hjerrild,M.,
            Wisniewski,J.R., Stahl,E., Bolouri,M.S., Ray,H.N., Sihag,S.,
            Kamal,M., Patterson,N., Lander,E.S. and Mann,M.
  TITLE     Integrated analysis of protein composition, tissue diversity, and
            gene regulation in mouse mitochondria
  JOURNAL   Cell 115 (5), 629-640 (2003)
   PUBMED   14651853
REFERENCE   4  (bases 1 to 1560)
  AUTHORS   Feng,Y., Hofstein,H.A., Zwahlen,J. and Tonge,P.J.
  TITLE     Effect of mutagenesis on the stereochemistry of enoyl-CoA hydratase
  JOURNAL   Biochemistry 41 (42), 12883-12890 (2002)
   PUBMED   12379132
REFERENCE   5  (bases 1 to 1560)
  AUTHORS   Engel,C.K., Kiema,T.R., Hiltunen,J.K. and Wierenga,R.K.
  TITLE     The crystal structure of enoyl-CoA hydratase complexed with
            octanoyl-CoA reveals the structural adaptations required for
            binding of a long chain fatty acid-CoA molecule
  JOURNAL   J Mol Biol 275 (5), 847-859 (1998)
   PUBMED   9480773
REFERENCE   6  (bases 1 to 1560)
  AUTHORS   Engel,C.K., Mathieu,M., Zeelen,J.P., Hiltunen,J.K. and
            Wierenga,R.K.
  TITLE     Crystal structure of enoyl-coenzyme A (CoA) hydratase at 2.5
            angstroms resolution: a spiral fold defines the CoA-binding pocket
  JOURNAL   EMBO J 15 (19), 5135-5145 (1996)
   PUBMED   8895557
REFERENCE   7  (bases 1 to 1560)
  AUTHORS   D'Ordine,R.L., Bahnson,B.J., Tonge,P.J. and Anderson,V.E.
  TITLE     Enoyl-coenzyme A hydratase-catalyzed exchange of the alpha-protons
            of coenzyme A thiol esters: a model for an enolized intermediate in
            the enzyme-catalyzed elimination?
  JOURNAL   Biochemistry 33 (49), 14733-14742 (1994)
   PUBMED   7993901
REFERENCE   8  (bases 1 to 1560)
  AUTHORS   Minami-Ishii,N., Taketani,S., Osumi,T. and Hashimoto,T.
  TITLE     Molecular cloning and sequence analysis of the cDNA for rat
            mitochondrial enoyl-CoA hydratase. Structural and evolutionary
            relationships linked to the bifunctional enzyme of the peroxisomal
            beta-oxidation system
  JOURNAL   Eur J Biochem 185 (1), 73-78 (1989)
   PUBMED   2806264
COMMENT     PROVISIONAL REFSEQ: This record has not yet been subject to final
            NCBI review. The reference sequence was derived from BC064655.1.
            
            On Feb 22, 2004 this sequence version replaced NM_078623.1.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC064655.1, X15958.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA5756307, SAMEA5760400
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            gene product(s) localized to mito. :: inferred from homology
            RefSeq Select criteria             :: based on single
                                                  protein-coding transcript
            ##RefSeq-Attributes-END##
FEATURES             Location/Qualifiers
     source          1..1560
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /db_xref="taxon:10116"
                     /chromosome="1"
                     /map="1q41"
     gene            1..1560
                     /gene="Echs1"
                     /gene_synonym="mECH; mECH1"
                     /note="enoyl-CoA hydratase, short chain 1"
                     /db_xref="GeneID:140547"
                     /db_xref="RGD:69330"
     exon            1..105
                     /gene="Echs1"
                     /gene_synonym="mECH; mECH1"
                     /inference="alignment:Splign:2.1.0"
     CDS             18..890
                     /gene="Echs1"
                     /gene_synonym="mECH; mECH1"
                     /EC_number="5.3.3.8"
                     /EC_number="4.2.1.17"
                     /note="mitochondrial short-chain enoyl-coenzyme A
                     hydratase 1; enoyl-CoA hydratase, mitochondrial; SCEH;
                     enoyl-CoA hydratase 1; short-chain enoyl-CoA hydratase;
                     enoyl Coenzyme A hydratase, short chain 1; enoyl-CoA
                     hydratase, mitochondrial-like; Enoyl-CoA hydratase, short
                     chain 1, mitochondrial; enoyl Coenzyme A hydratase, short
                     chain, 1, mitochondrial; enoyl CoA hydratase, short chain,
                     1, mitochondrial"
                     /codon_start=1
                     /product="enoyl-CoA hydratase, mitochondrial precursor"
                     /protein_id="NP_511178.1"
                     /db_xref="GeneID:140547"
                     /db_xref="RGD:69330"
                     /translation="MAALRALLPRACNSLLSPVRCPEFRRFASGANFQYIITEKKGKN
                     SSVGLIQLNRPKALNALCNGLIEELNQALETFEEDPAVGAIVLTGGEKAFAAGADIKE
                     MQNRTFQDCYSGKFLSHWDHITRIKKPVIAAVNGYALGGGCELAMMCDIIYAGEKAQF
                     GQPEILLGTIPGAGGTQRLTRAVGKSLAMEMVLTGDRISAQDAKQAGLVSKIFPVETL
                     VEEAIQCAEKIANNSKIIVAMAKESVNAAFEMTLTEGNKLEKKLFYSTFATDDRREGM
                     SAFVEKRKANFKDH"
     exon            106..303
                     /gene="Echs1"
                     /gene_synonym="mECH; mECH1"
                     /inference="alignment:Splign:2.1.0"
     exon            304..431
                     /gene="Echs1"
                     /gene_synonym="mECH; mECH1"
                     /inference="alignment:Splign:2.1.0"
     exon            432..531
                     /gene="Echs1"
                     /gene_synonym="mECH; mECH1"
                     /inference="alignment:Splign:2.1.0"
     exon            532..636
                     /gene="Echs1"
                     /gene_synonym="mECH; mECH1"
                     /inference="alignment:Splign:2.1.0"
     exon            637..756
                     /gene="Echs1"
                     /gene_synonym="mECH; mECH1"
                     /inference="alignment:Splign:2.1.0"
     exon            757..824
                     /gene="Echs1"
                     /gene_synonym="mECH; mECH1"
                     /inference="alignment:Splign:2.1.0"
     exon            825..1462
                     /gene="Echs1"
                     /gene_synonym="mECH; mECH1"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 caaatcgtct ctccgccatg gcggccctgc gtgctctgct gcccagagcc tgcaactcgc
       61 tgttgtcccc agttcgctgc ccagaattcc ggcgcttcgc ctcgggtgct aactttcagt
      121 acatcatcac ggaaaagaaa ggaaagaata gcagcgtggg gctgatccag ttgaaccgtc
      181 ccaaagcact caatgcactt tgcaatggac tgattgagga gctcaaccaa gcactggaga
      241 cctttgagga agatcccgct gtgggcgcca ttgtgctcac tggtggggag aaggcctttg
      301 cagccggagc tgacatcaag gaaatgcaga accggacatt tcaggactgt tactcaggca
      361 agttcctgag ccactgggac catatcaccc ggatcaagaa accggtcatc gcggctgtca
      421 atggctatgc tcttggtggg ggctgtgaac ttgccatgat gtgcgatatc atctatgctg
      481 gtgagaaagc ccagtttgga cagccagaaa tcctcctggg gaccatccca ggtgcagggg
      541 gcactcagag actcacccga gcagtcggca aatcactagc aatggagatg gtcctcactg
      601 gtgaccgaat ttcagcacag gatgccaagc aagcaggtct tgtaagcaag atttttcccg
      661 ttgaaacact ggttgaagag gccatccaat gtgcagaaaa gatcgccaac aattccaaga
      721 tcatagtagc catggcgaaa gaatctgtga atgcagcctt tgaaatgacg ttaacagaag
      781 gaaataagct ggagaagaag ctcttctatt ccacctttgc cactgatgac cggagagaag
      841 ggatgtctgc ctttgtggag aaaaggaagg ccaacttcaa agaccactga gagctggcag
      901 ccataccttt ctccaccctg gagaggaaag ctcagcctgt cagagggtag tagtgtctgc
      961 cacagttcct accgaaggct tcaaggctgt ggccacagct tgggtactcc tggtgtggcc
     1021 tgcaaccttc accagttgtt ggaagtcggt ctctcacagt tttctaaatc agaaatctgg
     1081 gaagctttca gggtgtcttg atttgtttaa cttcactcta aaggcagggc acgcttgggc
     1141 cagccacttc cctgtgcact atctgcagtg catagctcaa gatgtgagct tcaagaggga
     1201 agtggccctg cctgctgatt ccaagcacga gaattcaatg aaagtaactc cttcggttta
     1261 gtttcgactc gcctgtagtt gtctctctag agggtcaata tgttttttaa ttattattgc
     1321 tatgattttc ttatgggtag ggttttgctg tgtatccctg gctgcctgga agttactgta
     1381 tagaccaggt tgaccttgaa ttcacagaga tcaaccagcc tctgcttccc aagtattggg
     1441 attaaaggta tatgctacca caaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa
     1501 aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa
//
