LOCUS       NM_001100580            4766 bp    mRNA    linear   ROD 27-MAR-2024
DEFINITION  Rattus norvegicus E2F transcription factor 8 (E2f8), mRNA.
ACCESSION   NM_001100580 XM_001080259 XM_006229263
VERSION     NM_001100580.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 4766)
  AUTHORS   Chen,H.Z., Ouseph,M.M., Li,J., Pecot,T., Chokshi,V., Kent,L.,
            Bae,S., Byrne,M., Duran,C., Comstock,G., Trikha,P., Mair,M.,
            Senapati,S., Martin,C.K., Gandhi,S., Wilson,N., Liu,B., Huang,Y.W.,
            Thompson,J.C., Raman,S., Singh,S., Leone,M., Machiraju,R.,
            Huang,K., Mo,X., Fernandez,S., Kalaszczynska,I., Wolgemuth,D.J.,
            Sicinski,P., Huang,T., Jin,V. and Leone,G.
  TITLE     Canonical and atypical E2Fs regulate the mammalian endocycle
  JOURNAL   Nat Cell Biol 14 (11), 1192-1202 (2012)
   PUBMED   23064266
REFERENCE   2  (bases 1 to 4766)
  AUTHORS   Pandit,S.K., Westendorp,B., Nantasanti,S., van Liere,E.,
            Tooten,P.C., Cornelissen,P.W., Toussaint,M.J., Lamers,W.H. and de
            Bruin,A.
  TITLE     E2F8 is essential for polyploidization in mammalian cells
  JOURNAL   Nat Cell Biol 14 (11), 1181-1191 (2012)
   PUBMED   23064264
REFERENCE   3  (bases 1 to 4766)
  AUTHORS   Weijts,B.G., Bakker,W.J., Cornelissen,P.W., Liang,K.H.,
            Schaftenaar,F.H., Westendorp,B., de Wolf,C.A., Paciejewska,M.,
            Scheele,C.L., Kent,L., Leone,G., Schulte-Merker,S. and de Bruin,A.
  TITLE     E2F7 and E2F8 promote angiogenesis through transcriptional
            activation of VEGFA in cooperation with HIF1
  JOURNAL   EMBO J 31 (19), 3871-3884 (2012)
   PUBMED   22903062
REFERENCE   4  (bases 1 to 4766)
  AUTHORS   Ouseph,M.M., Li,J., Chen,H.Z., Pecot,T., Wenzel,P., Thompson,J.C.,
            Comstock,G., Chokshi,V., Byrne,M., Forde,B., Chong,J.L., Huang,K.,
            Machiraju,R., de Bruin,A. and Leone,G.
  TITLE     Atypical E2F repressors and activators coordinate placental
            development
  JOURNAL   Dev Cell 22 (4), 849-862 (2012)
   PUBMED   22516201
REFERENCE   5  (bases 1 to 4766)
  AUTHORS   Christensen,J., Cloos,P., Toftegaard,U., Klinkenberg,D.,
            Bracken,A.P., Trinh,E., Heeran,M., Di Stefano,L. and Helin,K.
  TITLE     Characterization of E2F8, a novel E2F-like cell-cycle regulated
            repressor of E2F-activated transcription
  JOURNAL   Nucleic Acids Res 33 (17), 5458-5470 (2005)
   PUBMED   16179649
  REMARK    Publication Status: Online-Only
REFERENCE   6  (bases 1 to 4766)
  AUTHORS   Maiti,B., Li,J., de Bruin,A., Gordon,F., Timmers,C., Opavsky,R.,
            Patil,K., Tuttle,J., Cleghorn,W. and Leone,G.
  TITLE     Cloning and characterization of mouse E2F8, a novel mammalian E2F
            family member capable of blocking cellular proliferation
  JOURNAL   J Biol Chem 280 (18), 18211-18220 (2005)
   PUBMED   15722552
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            JAXUCZ010000001.1.
            
            On Feb 11, 2021 this sequence version replaced NM_001100580.1.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR26643292.2225.1,
                                           SRR26643429.2040.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA5760383,
                                           SAMEA5760386 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-359               JAXUCZ010000001.1  107719651-107720009 c
            360-483             JAXUCZ010000001.1  107719260-107719383 c
            484-762             JAXUCZ010000001.1  107717368-107717646 c
            763-919             JAXUCZ010000001.1  107716883-107717039 c
            920-1252            JAXUCZ010000001.1  107713267-107713599 c
            1253-1414           JAXUCZ010000001.1  107711784-107711945 c
            1415-1552           JAXUCZ010000001.1  107710072-107710209 c
            1553-1753           JAXUCZ010000001.1  107708895-107709095 c
            1754-1841           JAXUCZ010000001.1  107708565-107708652 c
            1842-2385           JAXUCZ010000001.1  107707382-107707925 c
            2386-2518           JAXUCZ010000001.1  107704791-107704923 c
            2519-2919           JAXUCZ010000001.1  107704266-107704666 c
            2920-4766           JAXUCZ010000001.1  107701985-107703831 c
FEATURES             Location/Qualifiers
     source          1..4766
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="BN"
                     /db_xref="taxon:10116"
                     /chromosome="1"
                     /map="1q22"
     gene            1..4766
                     /gene="E2f8"
                     /gene_synonym="E2F-8; RGD1308091"
                     /note="E2F transcription factor 8"
                     /db_xref="GeneID:308607"
                     /db_xref="RGD:1308091"
     exon            1..359
                     /gene="E2f8"
                     /gene_synonym="E2F-8; RGD1308091"
                     /inference="alignment:Splign:2.1.0"
     exon            360..483
                     /gene="E2f8"
                     /gene_synonym="E2F-8; RGD1308091"
                     /inference="alignment:Splign:2.1.0"
     CDS             469..3102
                     /gene="E2f8"
                     /gene_synonym="E2F-8; RGD1308091"
                     /codon_start=1
                     /product="transcription factor E2F8"
                     /protein_id="NP_001094050.2"
                     /db_xref="GeneID:308607"
                     /db_xref="RGD:1308091"
                     /translation="MENQKENLFSEPHKRGLVKSPLQESSKANVVLAEIQPDLGPLTT
                     PTKPKEVSQGEPWTPTANLKMLISAVSPEIRSRDQKRGLSDNRSGLPEARDCLHEHLS
                     GDEFEKSQPSRKEKSLGLLCHKFLARYPKYPNPAVNNDICLDEVAEELNVERRRIYDI
                     VNVLESLHMVSRLAKNRYTWHGRHNLTKTLGTLKSVGEENKYAEQIMMIKRKEHEQEF
                     DFIKSCGLEDHVIKGDHVIKSTAGQNGHSDMCFVELPGVEFRAASANSRKDKSLRVMS
                     QKFVMLFLVSTPQIVSLEIAAKILIGEDHVEDLDKSKFKTKIRRLYDIANVLSSLDLI
                     KKVHVTEERGRKPAFKWTGPEISPNNSGSSPVMPLTASLEAEQSAKENCAKNLFSTRG
                     KPSFTRHPSLIKLVKSIENDRRKISSAPSSPVKSSKAESSQNSPPVPNKMAQLAAICK
                     MQLEEQSSEPRKRVKVNLTRSGHYKPLAPLDPAVNTELELLAPSLIQPLGMVPLIPSP
                     LSSAVPVILPQAPSGPSYAIYLQPAQAQMLTPPHGLSPTVCPTQSSNATGSKDPTDAP
                     TEKTATDATKSSASCRPGSLQPAPERQGAKNRSKETTGDRGTKRTGALEDGGPGPIKK
                     PKEDLKALENVPTPTTLFPSGYLIPLTQCPSLGPDPMLSNTENSGTLSPNHRIYGSPI
                     AGVIPVASSELTAVNFPPFHVTPLKLMVSPTSMAAVPVGNSPALSSSHPAPTQNPSSA
                     IVNFTLQHLGLISPGVQMSASPGPGAGTVPLSPRVEAASVIPDNLSSRQGRATIHDSP
                     VLGQSQLNGQPVAGTGAQQPVPVTPKGSQLVAESFFRTPGGPTKPTSSSFMDFDGANK
                     TSFGTLFVPQRKLEVSTEDVH"
     exon            484..762
                     /gene="E2f8"
                     /gene_synonym="E2F-8; RGD1308091"
                     /inference="alignment:Splign:2.1.0"
     exon            763..919
                     /gene="E2f8"
                     /gene_synonym="E2F-8; RGD1308091"
                     /inference="alignment:Splign:2.1.0"
     exon            920..1252
                     /gene="E2f8"
                     /gene_synonym="E2F-8; RGD1308091"
                     /inference="alignment:Splign:2.1.0"
     exon            1253..1414
                     /gene="E2f8"
                     /gene_synonym="E2F-8; RGD1308091"
                     /inference="alignment:Splign:2.1.0"
     exon            1415..1552
                     /gene="E2f8"
                     /gene_synonym="E2F-8; RGD1308091"
                     /inference="alignment:Splign:2.1.0"
     exon            1553..1753
                     /gene="E2f8"
                     /gene_synonym="E2F-8; RGD1308091"
                     /inference="alignment:Splign:2.1.0"
     exon            1754..1841
                     /gene="E2f8"
                     /gene_synonym="E2F-8; RGD1308091"
                     /inference="alignment:Splign:2.1.0"
     exon            1842..2385
                     /gene="E2f8"
                     /gene_synonym="E2F-8; RGD1308091"
                     /inference="alignment:Splign:2.1.0"
     exon            2386..2518
                     /gene="E2f8"
                     /gene_synonym="E2F-8; RGD1308091"
                     /inference="alignment:Splign:2.1.0"
     exon            2519..2919
                     /gene="E2f8"
                     /gene_synonym="E2F-8; RGD1308091"
                     /inference="alignment:Splign:2.1.0"
     exon            2920..4766
                     /gene="E2f8"
                     /gene_synonym="E2F-8; RGD1308091"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 cgggctacta cgggggactg tacctgggac ccggagcgca gcttaccgta cgccttggcg
       61 tcgcggtgat ttcggcatct agtgaacccc tacctcgccc tagtacttcg cgtgttgaaa
      121 gaagcctaaa aaaggaggtc cgaatcccaa agctctctgt aaatgccccg tcctgcgctt
      181 agagcgcaga gactgaattg gagggttgtt ctcaggccac ttcccaagtc cttccctctg
      241 agcctgtgca cgtgtgtgtc aggcgagaaa cttcaggatc tcccccgatg caggctcggt
      301 gtgcgctcct tcggaacccg ggctcgtgcg ctcagtccgc agcccggatc agtgcacagg
      361 gtagtaaaaa tctttaatcg attggactac ttaaaccatt ggaatttggg gaggaacttc
      421 agatttttcg tttttaaaga cacactaaat gtatgaggaa tcgacaaaat ggagaaccaa
      481 aaggaaaacc tcttttctga gccacataaa aggggactgg tgaaaagccc cctgcaagag
      541 tcctcgaagg ccaacgtggt actggctgag atccagcctg acctgggtcc tttaaccaca
      601 ccaaccaagc ccaaggaagt ctcccaagga gagccatgga cacccacagc caacctgaaa
      661 atgcttatca gcgcggtgag cccagagatc cgaagccgag atcagaaaag gggcctgtcc
      721 gacaaccgca gtggattacc cgaagctaga gactgtttgc atgaacactt atctggagat
      781 gaatttgaga agtcccagcc aagtcggaag gagaagagct tggggttgct atgccacaaa
      841 ttcttagcga ggtaccctaa gtaccccaac cccgctgtga ataacgacat ctgcctggac
      901 gaggtggccg aggagctcaa tgtcgaacgt cggcggattt atgacatcgt gaatgtacta
      961 gagagcctgc acatggtgag ccgcctcgcc aaaaacaggt acacttggca cggccggcac
     1021 aatctcacca aaaccctggg gacgctgaag agtgtcgggg aagagaacaa gtacgcggag
     1081 cagatcatga tgatcaaaag gaaagagcat gagcaagagt tcgacttcat taagagctgt
     1141 ggcctagagg accacgtgat caagggggac cacgtgatca agtcaaccgc tggccagaac
     1201 gggcactcag acatgtgctt cgtagagctc cctggagtgg aatttcgggc agcttctgca
     1261 aacagccgca aagacaagtc cttacgcgtg atgagccaga agtttgtgat gctgttcttg
     1321 gtgtcgacgc ctcagatagt aagcctggag atcgctgcca agatcttaat tggggaggac
     1381 cacgtggaag atctggataa aagcaagttt aaaacaaaaa ttaggaggct gtacgatatt
     1441 gctaatgttc tgagtagctt ggatcttatc aagaaagttc atgttacaga agaacgagga
     1501 cggaagccag ctttcaaatg gacaggcccg gaaatcagcc caaataacag tggctccagc
     1561 ccagtcatgc ctctcaccgc ctccttagag gctgagcagt ctgcaaaaga gaactgtgcc
     1621 aaaaacctct tttctacacg ggggaaaccc agctttactc gacacccatc tcttatcaaa
     1681 ttggtaaaga gcatagaaaa tgatcggagg aagatcagtt ccgctcccag cagccctgtc
     1741 aagagcagca aagctgagag ttctcaaaat tctccacccg tcccaaacaa aatggcccag
     1801 ctcgctgcta tctgcaagat gcagttggag gagcagtcaa gtgaacccag gaagagagta
     1861 aaagtcaacc taacaagatc tgggcactac aaaccactgg ctcccctgga ccctgcagtg
     1921 aacactgagc tggaactgct tgctccgtcc cttatccagc ccctgggcat ggtccccctg
     1981 atccctagcc cattgtcatc tgcagtgcct gtgatcctac ctcaggcccc ttcgggccca
     2041 tcctatgcca tctacttgca acctgcccaa gcccaaatgt tgacaccacc ccatggcctg
     2101 agcccaacag tctgccccac ccagtcctct aatgctaccg gatccaaaga ccctacagac
     2161 gcccccactg agaagacggc cacggatgcc acaaagtcca gtgcctcctg cagacccgga
     2221 agcttgcagc cagcaccaga aaggcaaggt gctaagaatc gaagcaagga gacgaccgga
     2281 gatcgaggca cgaagaggac gggcgccttg gaggacggtg gtcccggtcc cataaagaaa
     2341 cctaaagagg acctgaaggc gcttgagaat gtccccaccc ccacgaccct gttcccatca
     2401 ggatacctaa tccctcttac ccagtgccca tccctggggc cagaccctat gttgtctaac
     2461 acagaaaact caggtacact ttccccaaac cacaggatct acggctcccc aattgcaggt
     2521 gtgatcccag tggcgtcgtc tgaactcact gctgtgaatt ttcccccatt ccatgtgaca
     2581 ccgctgaaac ttatggtctc cccgacgtct atggcagccg tacctgttgg gaacagcccg
     2641 gctctcagct ccagccaccc tgctcccacc cagaacccaa gctcagccat tgtaaacttt
     2701 acactgcagc acttgggact catctccccc ggcgtgcaga tgtctgccag ccccgggcct
     2761 ggagctggca cagtccctct gtccccacgt gtagaagcag ctagcgtcat accagataac
     2821 ttgagctctc ggcaagggag ggccaccatc catgactctc cagtcctggg ccaaagccaa
     2881 ctaaatggac aaccagttgc tgggacaggg gcacagcagc ctgttcctgt gacgcccaaa
     2941 ggctcccagt tggtggctga aagtttcttc cgtactccag gtggaccaac caagcccacc
     3001 agctcatctt tcatggattt cgatggtgct aacaaaacct cctttggaac cctctttgtc
     3061 ccacagcgca aactggaagt ctcaactgag gatgtccatt gattgacaga tggtggttta
     3121 atttaattct ctaaagagct atttaatgag acgatataca catacgaatt gagagagaac
     3181 accaccttta aaaatactgt aaatacttgg ggttttactc aatatcacag cagatatttg
     3241 tcatgcttac acacatgcac acatacacac acacacacac acacacacac acatgcatac
     3301 atacatactc acacatttgt acaaggccaa attcagcctt cagtactata aaataagatg
     3361 ttgaactaag gtacattaac ttctgggggt gggcgggttc atttttttca cctgtgcctc
     3421 tattattatg caaagtaact ttactaaagt ttacttccct ctcagcaaat gtttgagtta
     3481 ccgaacacag cacctgctca aactctttgc acaaaggaaa tgctgtgtga tgtataatgt
     3541 atttttacac agggaggcga tagctatttt tttttgtaat ttctttaatc ctttgctgtt
     3601 tcagtctgtc tttttgtaga gtttacagtc ctcaatcaag tctatggaaa aaattattta
     3661 taaaatgtat ttttaatcat aagttgttga aattaaaact cttctaaaat gtagttaaca
     3721 ctttcgtttg gccacatagg gcactcactg tcgagagaaa ctgataactt atctaacctc
     3781 agaggccggt tcttgggaat gacttccttg gacacccggt cacaggaagg tttcttctgt
     3841 actccaaccc cttcccctgc cacaatccag agcattgcta ggatagtgaa tgacggtgta
     3901 gagaggctaa gagaactggt gaaagacagg aggagctaag gtagtccccg tctctgccca
     3961 gcacccatcc cagacctgct atgcagagga gaggggcagc cactgtccca tatcttctct
     4021 atggtccagc tccagagaag gaattgcagt aaggattctc tcctcttggc agagaaccaa
     4081 cacatactac accccagtga gctctagaga aagagtaagc tatggcgtgc tcaaggtgca
     4141 tcttgacccc ttaaaggaca ggccacatgc tgagaagaca agagccgcct tttgtgaccc
     4201 cagtctttca ggaacatcat atctgtttcc tatgtgctaa tgttgggaac tacagacatt
     4261 tgaattgcgt gttaccgagc ccagatcaca aggccactga ggtagaattt aaggcaacaa
     4321 gtctacagtc tttgccagga ctcaaccttc ccatccaact cagtgaaact gaacagctat
     4381 gggaggctct gcactgttct ggacattcac agggtcacct atctttaaag atgcattctg
     4441 tatggccaag cccaggtaag atgacagatt ttcctgttgg tctagagagg tagcccattg
     4501 tagacaggtt ggaagccaat gttctgctct gattttgtta tcctccatgt gaagaaactc
     4561 cctggaaaag ccagatgccc ctctttccta agagttatgt tagctatcga gacagctcgg
     4621 ggcagaatct ctcagctgta tcccagaatt ccgagacttc cccctcgatc actgcctcct
     4681 gggctctgca cccttctcct tgagtgtggg taggccctgt gccttgctcc cgatcaagaa
     4741 accattaaat gaatggggtg tgtata
//
