LOCUS       NM_001040271             812 bp    mRNA    linear   ROD 22-SEP-2024
DEFINITION  Rattus norvegicus deoxyuridine triphosphatase (Dut), transcript
            variant 2, mRNA.
ACCESSION   NM_001040271
VERSION     NM_001040271.2
KEYWORDS    RefSeq.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 812)
  AUTHORS   Morgenstern,M., Peikert,C.D., Lubbert,P., Suppanz,I., Klemm,C.,
            Alka,O., Steiert,C., Naumenko,N., Schendzielorz,A., Melchionda,L.,
            Muhlhauser,W.W.D., Knapp,B., Busch,J.D., Stiller,S.B.,
            Dannenmaier,S., Lindau,C., Licheva,M., Eickhorst,C., Galbusera,R.,
            Zerbes,R.M., Ryan,M.T., Kraft,C., Kozjak-Pavlovic,V., Drepper,F.,
            Dennerlein,S., Oeljeklaus,S., Pfanner,N., Wiedemann,N. and
            Warscheid,B.
  TITLE     Quantitative high-confidence human mitochondrial proteome and its
            dynamics in cellular context
  JOURNAL   Cell Metab 33 (12), 2464-2483 (2021)
   PUBMED   34800366
REFERENCE   2  (bases 1 to 812)
  AUTHORS   Dos Santos,R.S., Daures,M., Philippi,A., Romero,S., Marselli,L.,
            Marchetti,P., Senee,V., Bacq,D., Besse,C., Baz,B., Marroqui,L.,
            Ivanoff,S., Masliah-Planchon,J., Nicolino,M., Soulier,J., Socie,G.,
            Eizirik,D.L., Gautier,J.F. and Julier,C.
  TITLE     dUTPase (DUT) Is Mutated in a Novel Monogenic Syndrome With
            Diabetes and Bone Marrow Failure
  JOURNAL   Diabetes 66 (4), 1086-1096 (2017)
   PUBMED   28073829
  REMARK    GeneRIF: DUT inhibition by two independent siRNAs increased
            apoptosis in beta-cells.
REFERENCE   3  (bases 1 to 812)
  AUTHORS   Castello,A., Fischer,B., Eichelbaum,K., Horos,R., Beckmann,B.M.,
            Strein,C., Davey,N.E., Humphreys,D.T., Preiss,T., Steinmetz,L.M.,
            Krijgsveld,J. and Hentze,M.W.
  TITLE     Insights into RNA biology from an atlas of mammalian mRNA-binding
            proteins
  JOURNAL   Cell 149 (6), 1393-1406 (2012)
   PUBMED   22658674
REFERENCE   4  (bases 1 to 812)
  AUTHORS   Buschow,S.I., van Balkom,B.W., Aalberts,M., Heck,A.J., Wauben,M.
            and Stoorvogel,W.
  TITLE     MHC class II-associated proteins in B-cell exosomes and potential
            functional implications for exosome biogenesis
  JOURNAL   Immunol Cell Biol 88 (8), 851-856 (2010)
   PUBMED   20458337
REFERENCE   5  (bases 1 to 812)
  AUTHORS   Romeike,B.F., Bockeler,A., Kremmer,E., Sommer,P., Krick,C. and
            Grasser,F.
  TITLE     Immunohistochemical detection of dUTPase in intracranial tumors
  JOURNAL   Pathol Res Pract 201 (11), 727-732 (2005)
   PUBMED   16325515
  REMARK    Erratum:[Pathol Res Pract. 2006;202(1):65]
REFERENCE   6  (bases 1 to 812)
  AUTHORS   Chu,R., Lin,Y., Rao,M.S. and Reddy,J.K.
  TITLE     Cloning and identification of rat deoxyuridine triphosphatase as an
            inhibitor of peroxisome proliferator-activated receptor alpha
  JOURNAL   J Biol Chem 271 (44), 27670-27676 (1996)
   PUBMED   8910358
REFERENCE   7  (bases 1 to 812)
  AUTHORS   Ladner,R.D., McNulty,D.E., Carr,S.A., Roberts,G.D. and
            Caradonna,S.J.
  TITLE     Characterization of distinct nuclear and mitochondrial forms of
            human deoxyuridine triphosphate nucleotidohydrolase
  JOURNAL   J Biol Chem 271 (13), 7745-7751 (1996)
   PUBMED   8631816
REFERENCE   8  (bases 1 to 812)
  AUTHORS   Yamaguchi,M. and Sakurai,T.
  TITLE     Reversible effect of calcium-binding protein regucalcin on the
            Ca(2+)-induced inhibition of deoxyuridine 5'-triphosphatase
            activity in rat liver cytosol
  JOURNAL   Mol Cell Biochem 110 (1), 25-29 (1992)
   PUBMED   1315924
REFERENCE   9  (bases 1 to 812)
  AUTHORS   Hokari,S., Hasegawa,M., Sakagishi,Y. and Kikuchi,G.
  TITLE     Deoxyuridine triphosphate nucleotidohydrolase activity and its
            correlation with multiplication of erythroid cells in rat spleen
  JOURNAL   Biochem Int 14 (5), 851-857 (1987)
   PUBMED   2841940
REFERENCE   10 (bases 1 to 812)
  AUTHORS   Hokari,S. and Sakagishi,Y.
  TITLE     Purification and characterization of deoxyuridine triphosphate
            nucleotidohydrolase from anemic rat spleen: a trimer composition of
            the enzyme protein
  JOURNAL   Arch Biochem Biophys 253 (2), 350-356 (1987)
   PUBMED   3032103
COMMENT     PROVISIONAL REFSEQ: This record has not yet been subject to final
            NCBI review. The reference sequence was derived from
            JAXUCZ010000003.1.
            
            On Nov 27, 2020 this sequence version replaced NM_001040271.1.
            
            Transcript Variant: This variant (2) differs in the 5' UTR and 5'
            CDS compared to variant 1. The resulting protein (isoform 2) is
            shorter and has a distinct N-terminus compared to isoform 1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: CB325379.1, CK840877.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA5760383, SAMEA5760386
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            gene product(s) localized to mito. :: inferred from homology
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-171               JAXUCZ010000003.1  132952899-132953069
            172-263             JAXUCZ010000003.1  132954755-132954846
            264-308             JAXUCZ010000003.1  132956823-132956867
            309-383             JAXUCZ010000003.1  132962559-132962633
            384-454             JAXUCZ010000003.1  132962765-132962835
            455-812             JAXUCZ010000003.1  132963073-132963430
FEATURES             Location/Qualifiers
     source          1..812
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="BN"
                     /db_xref="taxon:10116"
                     /chromosome="3"
                     /map="3q36"
     gene            1..812
                     /gene="Dut"
                     /gene_synonym="Dutp; PIP4"
                     /note="deoxyuridine triphosphatase"
                     /db_xref="GeneID:497778"
                     /db_xref="RGD:620849"
     exon            1..171
                     /gene="Dut"
                     /gene_synonym="Dutp; PIP4"
                     /inference="alignment:Splign:2.1.0"
     CDS             26..511
                     /gene="Dut"
                     /gene_synonym="Dutp; PIP4"
                     /EC_number="3.6.1.23"
                     /note="isoform 2 is encoded by transcript variant 2;
                     deoxyuridine 5'-triphosphate nucleotidohydrolase; dUTPase;
                     dUTP pyrophosphatase; PPAR-interacting protein 4"
                     /codon_start=1
                     /product="deoxyuridine 5'-triphosphate nucleotidohydrolase
                     isoform 2"
                     /protein_id="NP_001035361.1"
                     /db_xref="GeneID:497778"
                     /db_xref="RGD:620849"
                     /translation="MPCSEDPAVSVSKRARAEDDASLRFVRLSEHATAPTRGSARAAG
                     YDLYSAYDYTIPSMEKALVKTDIQIAVPSGCYGRVAPRSGLAVKHFIDVGAGVIDEDY
                     RGNVGVVLFNFGKEKFEVKKGDRIAQLICERILYPDLEEVQTLDNTERGSGGFGSTGK
                     N"
     exon            172..263
                     /gene="Dut"
                     /gene_synonym="Dutp; PIP4"
                     /inference="alignment:Splign:2.1.0"
     exon            264..308
                     /gene="Dut"
                     /gene_synonym="Dutp; PIP4"
                     /inference="alignment:Splign:2.1.0"
     exon            309..383
                     /gene="Dut"
                     /gene_synonym="Dutp; PIP4"
                     /inference="alignment:Splign:2.1.0"
     exon            384..454
                     /gene="Dut"
                     /gene_synonym="Dutp; PIP4"
                     /inference="alignment:Splign:2.1.0"
     exon            455..812
                     /gene="Dut"
                     /gene_synonym="Dutp; PIP4"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 tttctgcatt taccttccag ccgccatgcc ctgctcggaa gaccctgcgg tctctgtctc
       61 taagagggct cgagcggaag atgacgcttc tctgcgtttc gtgcggctct cggagcacgc
      121 tacggccccc acccgcgggt ccgcgcgcgc tgcaggctac gacctgtaca gtgcctatga
      181 ttatacaatt ccatccatgg agaaagccct tgtgaagaca gacattcaga tagctgttcc
      241 ttctgggtgc tatggaagag tggctccacg ttctggcttg gctgtaaagc acttcataga
      301 tgtaggagcc ggtgtcatag atgaagatta tagaggaaat gtcggcgtcg tgctgttcaa
      361 ttttggaaaa gagaagttcg aggtgaaaaa aggtgaccgg atcgcgcagc tcatttgtga
      421 gcggattctt tatccagact tagaggaggt gcaaacactg gataacactg agagaggctc
      481 aggaggcttc ggctccacgg gaaagaatta aaactttgct tgaagtactt tgctgtttga
      541 acactgtaaa cgagctttaa tttcagaagc attttgtgtt ctaggatgta ggaaaggata
      601 cctatcacgg tggaactgct ctgttctcta gttgatggtt gcctgtaaat ctgtatcgtg
      661 agaatggcat tgacatgcag acttggttat tagatatttt ttttaaattt ggtgttgtat
      721 ttaaattata gtcctataag aactgatttt gctctaattg ctatattatt tgtaattttt
      781 ttttttttat ccaataaagt ccgaagtctt ta
//
