LOCUS       NM_031705               2079 bp    mRNA    linear   ROD 31-MAR-2024
DEFINITION  Rattus norvegicus dihydropyrimidinase (Dpys), mRNA.
ACCESSION   NM_031705
VERSION     NM_031705.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 2079)
  AUTHORS   Gonzales,P.A., Pisitkun,T., Hoffert,J.D., Tchapyjnikov,D.,
            Star,R.A., Kleta,R., Wang,N.S. and Knepper,M.A.
  TITLE     Large-scale proteomics and phosphoproteomics of urinary exosomes
  JOURNAL   J Am Soc Nephrol 20 (2), 363-379 (2009)
   PUBMED   19056867
REFERENCE   2  (bases 1 to 2079)
  AUTHORS   Thomas,H.R., Ezzeldin,H.H., Guarcello,V., Mattison,L.K.,
            Fridley,B.L. and Diasio,R.B.
  TITLE     Genetic regulation of dihydropyrimidinase and its possible
            implication in altered uracil catabolism
  JOURNAL   Pharmacogenet Genomics 17 (11), 973-987 (2007)
   PUBMED   18075467
REFERENCE   3  (bases 1 to 2079)
  AUTHORS   Fukada,M., Watakabe,I., Yuasa-Kawada,J., Kawachi,H., Kuroiwa,A.,
            Matsuda,Y. and Noda,M.
  TITLE     Molecular characterization of CRMP5, a novel member of the
            collapsin response mediator protein family
  JOURNAL   J Biol Chem 275 (48), 37957-37965 (2000)
   PUBMED   10956643
REFERENCE   4  (bases 1 to 2079)
  AUTHORS   Van Kuilenburg,A.B., Van Lenthe,H. and Van Gennip,A.H.
  TITLE     Radiochemical assay for determination of dihydropyrimidinase
            activity using reversed-phase high-performance liquid
            chromatography
  JOURNAL   J Chromatogr B Biomed Sci Appl 729 (1-2), 307-314 (1999)
   PUBMED   10410956
REFERENCE   5  (bases 1 to 2079)
  AUTHORS   Hamajima,N., Kouwaki,M., Vreken,P., Matsuda,K., Sumi,S., Imaeda,M.,
            Ohba,S., Kidouchi,K., Nonaka,M., Sasaki,M., Tamaki,N., Endo,Y., De
            Abreu,R., Rotteveel,J., van Kuilenburg,A., van Gennip,A., Togari,H.
            and Wada,Y.
  TITLE     Dihydropyrimidinase deficiency: structural organization,
            chromosomal localization, and mutation analysis of the human
            dihydropyrimidinase gene
  JOURNAL   Am J Hum Genet 63 (3), 717-726 (1998)
   PUBMED   9718352
REFERENCE   6  (bases 1 to 2079)
  AUTHORS   Matsuda,K., Sakata,S., Kaneko,M., Hamajima,N., Nonaka,M., Sasaki,M.
            and Tamaki,N.
  TITLE     Molecular cloning and sequencing of a cDNA encoding
            dihydropyrimidinase from the rat liver
  JOURNAL   Biochim Biophys Acta 1307 (2), 140-144 (1996)
   PUBMED   8679696
REFERENCE   7  (bases 1 to 2079)
  AUTHORS   Tamaki,N., Fujimoto-Sakata,S., Kikugawa,M., Kaneko,M., Onosaka,S.
            and Takagi,T.
  TITLE     Analysis of cyclic feed intake in rats fed on a zinc-deficient diet
            and the level of dihydropyrimidinase (EC 3.5.2.2)
  JOURNAL   Br J Nutr 73 (5), 711-722 (1995)
   PUBMED   7626590
REFERENCE   8  (bases 1 to 2079)
  AUTHORS   Kikugawa,M., Kaneko,M., Fujimoto-Sakata,S., Maeda,M., Kawasaki,K.,
            Takagi,T. and Tamaki,N.
  TITLE     Purification, characterization and inhibition of
            dihydropyrimidinase from rat liver
  JOURNAL   Eur J Biochem 219 (1-2), 393-399 (1994)
   PUBMED   8307005
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            JAXUCZ010000007.1.
            
            On Nov 24, 2020 this sequence version replaced NM_031705.1.
            
            ##Evidence-Data-START##
            Transcript exon combination :: D63704.1, BC081768.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA5760383,
                                           SAMEA5760389 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-347               JAXUCZ010000007.1  72813790-72814136   c
            348-506             JAXUCZ010000007.1  72801129-72801287   c
            507-686             JAXUCZ010000007.1  72798294-72798473   c
            687-876             JAXUCZ010000007.1  72796366-72796555   c
            877-1033            JAXUCZ010000007.1  72783218-72783374   c
            1034-1175           JAXUCZ010000007.1  72782340-72782481   c
            1176-1318           JAXUCZ010000007.1  72779833-72779975   c
            1319-1526           JAXUCZ010000007.1  72745798-72746005   c
            1527-1672           JAXUCZ010000007.1  72737943-72738088   c
            1673-2079           JAXUCZ010000007.1  72736150-72736556   c
FEATURES             Location/Qualifiers
     source          1..2079
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="BN"
                     /db_xref="taxon:10116"
                     /chromosome="7"
                     /map="7q31"
     gene            1..2079
                     /gene="Dpys"
                     /gene_synonym="DHP"
                     /note="dihydropyrimidinase"
                     /db_xref="GeneID:65135"
                     /db_xref="RGD:68376"
     exon            1..347
                     /gene="Dpys"
                     /gene_synonym="DHP"
                     /inference="alignment:Splign:2.1.0"
     CDS             84..1643
                     /gene="Dpys"
                     /gene_synonym="DHP"
                     /EC_number="3.5.2.2"
                     /note="DHPase; hydantoinase; dihydropyrimidine
                     amidohydrolase"
                     /codon_start=1
                     /product="dihydropyrimidinase"
                     /protein_id="NP_113893.2"
                     /db_xref="GeneID:65135"
                     /db_xref="RGD:68376"
                     /translation="MAPQERLLIRGGRVVNDDFSQVADVLVEDGVVRALGRDLLPPGD
                     TSRGLRILDAAGKLVLPGGIDTHTHMQFPFMGSQSVDDFHQGTKAALAGGTTMIIDFA
                     IPQKGSSLIEAFETWRNWADPKVCCDYSLHVAVTWWSDKVKEEMKTLAQDKGVNSFKM
                     FMAYKDLYMVQDQQMYAAFSQCKEIGAIAQVHAENGDLIAEGAKKMLALGITGPEGHE
                     LCRPEAVEAEATLRAITIASAVNCPLYIVHVMSKSAAKVIADAKREGKVVYGEPIAAG
                     LGTDGTQYWNKEWRHAAHHVMGPPLRPDPSTPGFLMNLLANGDLTTTGSDNCTFNTCQ
                     KALGKDDFTKIPNGVNGVEDRMSVIWEKGVHSGKMDENRFVAVTSTNAAKIFNLYPKK
                     GRIAVGSDADIVIWDPEATRTISAKTHHQAVNFNIFEGMVCHGVPLVTISRGRVVYEA
                     GVFDVTAGHGKFIPRQPFAEFIYKRVKQRDQTCTPIPVKRAPYKGEVITLKPRETKED
                     DTAGTRMQGHS"
     exon            348..506
                     /gene="Dpys"
                     /gene_synonym="DHP"
                     /inference="alignment:Splign:2.1.0"
     exon            507..686
                     /gene="Dpys"
                     /gene_synonym="DHP"
                     /inference="alignment:Splign:2.1.0"
     exon            687..876
                     /gene="Dpys"
                     /gene_synonym="DHP"
                     /inference="alignment:Splign:2.1.0"
     exon            877..1033
                     /gene="Dpys"
                     /gene_synonym="DHP"
                     /inference="alignment:Splign:2.1.0"
     exon            1034..1175
                     /gene="Dpys"
                     /gene_synonym="DHP"
                     /inference="alignment:Splign:2.1.0"
     exon            1176..1318
                     /gene="Dpys"
                     /gene_synonym="DHP"
                     /inference="alignment:Splign:2.1.0"
     exon            1319..1526
                     /gene="Dpys"
                     /gene_synonym="DHP"
                     /inference="alignment:Splign:2.1.0"
     exon            1527..1672
                     /gene="Dpys"
                     /gene_synonym="DHP"
                     /inference="alignment:Splign:2.1.0"
     exon            1673..2079
                     /gene="Dpys"
                     /gene_synonym="DHP"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 ccagcgggag agggagtttg gcacagtttg tggagaactc aaagaaaaac caactctgtt
       61 cgcagtcccc agctcctcca gccatggcac cacaagaacg acttctcatc cgcgggggtc
      121 gcgtggtcaa tgatgacttc tcacaggtgg ccgacgtgct agtggaggac ggcgtggtgc
      181 gggcgctggg acgggacttg ctgcctcccg gggacacatc ccgggggctg cggatcctag
      241 atgcagcggg caagctcgtc ctgccgggag gcatcgacac acacacgcac atgcagttcc
      301 cgttcatggg ctcgcagtca gtcgacgact tccaccaggg caccaaggct gctttggcag
      361 gaggcaccac catgatcatt gattttgcga ttcctcagaa aggcagctcc ctcattgaag
      421 cttttgagac ctggcgcaac tgggcagacc ccaaagtctg ctgtgactat agcctgcacg
      481 tggcagtgac atggtggagt gacaaggtaa aagaagaaat gaaaaccctt gcccaagata
      541 aaggcgttaa ctctttcaag atgtttatgg cctacaaaga cctgtacatg gtgcaagacc
      601 agcaaatgta cgctgccttt tctcagtgca aggagatagg ggccattgct caggtgcatg
      661 ccgagaatgg agatttgatt gcagaggggg ccaagaagat gctggcactg gggataacgg
      721 gccccgaggg gcacgagctg tgccgcccgg aagcagtgga ggcagaggcc accttgagag
      781 ccatcaccat tgctagcgct gtgaactgcc ctctatacat cgtgcacgtg atgagcaaat
      841 ccgcagcgaa ggtgatagct gatgcgaaga gagaaggaaa ggtggtctat ggagaaccaa
      901 ttgcagcagg tctgggcacg gatggcactc agtactggaa taaagaatgg cgccatgcag
      961 cccaccatgt catgggtccc ccactgagac ctgatccatc aacgcctggc tttctcatga
     1021 atctgttggc taatggcgat ctgaccacaa cagggagtga caactgcact ttcaacacct
     1081 gccaaaaagc tctagggaag gatgacttca ctaagattcc caatggggtg aatggtgtcg
     1141 aggacaggat gtcggtgata tgggaaaagg gcgtgcacag tggcaaaatg gatgaaaata
     1201 gatttgtggc agttaccagc acaaatgcag ccaaaatctt taatctttat ccgaaaaaag
     1261 gaagaatagc tgtaggctca gatgctgaca ttgtgatctg ggacccagaa gccaccagga
     1321 cgatctcagc caaaacacat catcaggccg ttaacttcaa cattttcgag ggcatggttt
     1381 gccatggggt gcccctggtg actatttcaa gaggcagagt ggtgtatgaa gcaggcgttt
     1441 tcgacgtcac agcaggacac gggaagttta ttccgcgaca acctttcgct gagttcattt
     1501 acaaacgagt caagcagcga gaccagacct gcacacctat accggtgaag cgtgcgccct
     1561 acaagggaga agtcatcaca ttgaaaccca gagagacaaa agaagacgac acagctggga
     1621 ccaggatgca gggccattcc tgatttggat gctggggtta agcaagtgca aacagagtga
     1681 aggctcccag gctcccctcc ccaccatgct gccaagccct ggagaagcac ctgccatttc
     1741 aactctccaa gatcctttag gaaaaatcat cgctctaggc ctctttgatt tttctctcag
     1801 aagcaatagc agcctgcctc accctgcctt tgttgctgtg gaagattgag gatataaatg
     1861 aacattattg ggatgaaacg tctgcatgaa gattcattga agattccttt ttcaaatgcc
     1921 atctctcctt accctagatt tctttctttg ggtttttaaa gatttccttc tggtgtaaag
     1981 gttttgtttg tttgttttgt tttaatgttt atgtttgttt taaaaatcag tgattttaca
     2041 tttcattgca actaataaac atctggagct tcattctgc
//
