LOCUS       NM_199388               1805 bp    mRNA    linear   ROD 27-MAR-2024
DEFINITION  Rattus norvegicus dolichyl-phosphate
            N-acetylglucosaminephosphotransferase 1 (Dpagt1), mRNA.
ACCESSION   NM_199388 XM_236188
VERSION     NM_199388.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 1805)
  AUTHORS   Yoo,J., Mashalidis,E.H., Kuk,A.C.Y., Yamamoto,K., Kaeser,B.,
            Ichikawa,S. and Lee,S.Y.
  TITLE     GlcNAc-1-P-transferase-tunicamycin complex structure reveals basis
            for inhibition of N-glycosylation
  JOURNAL   Nat Struct Mol Biol 25 (3), 217-224 (2018)
   PUBMED   29459785
REFERENCE   2  (bases 1 to 1805)
  AUTHORS   Nita-Lazar,M., Noonan,V., Rebustini,I., Walker,J., Menko,A.S. and
            Kukuruzinska,M.A.
  TITLE     Overexpression of DPAGT1 leads to aberrant N-glycosylation of
            E-cadherin and cellular discohesion in oral cancer
  JOURNAL   Cancer Res 69 (14), 5673-5680 (2009)
   PUBMED   19549906
REFERENCE   3  (bases 1 to 1805)
  AUTHORS   Wu,X., Rush,J.S., Karaoglu,D., Krasnewich,D., Lubinsky,M.S.,
            Waechter,C.J., Gilmore,R. and Freeze,H.H.
  TITLE     Deficiency of UDP-GlcNAc:Dolichol Phosphate N-Acetylglucosamine-1
            Phosphate Transferase (DPAGT1) causes a novel congenital disorder
            of Glycosylation Type Ij
  JOURNAL   Hum Mutat 22 (2), 144-150 (2003)
   PUBMED   12872255
REFERENCE   4  (bases 1 to 1805)
  AUTHORS   Eckert,V., Blank,M., Mazhari-Tabrizi,R., Mumberg,D., Funk,M. and
            Schwarz,R.T.
  TITLE     Cloning and functional expression of the human GlcNAc-1-P
            transferase, the enzyme for the committed step of the dolichol
            cycle, by heterologous complementation in Saccharomyces cerevisiae
  JOURNAL   Glycobiology 8 (1), 77-85 (1998)
   PUBMED   9451016
REFERENCE   5  (bases 1 to 1805)
  AUTHORS   Yasugi,E., Nakasuji,M., Dohi,T. and Oshima,M.
  TITLE     Major defect of carbohydrate-deficient-glycoprotein syndrome is not
            found in the synthesis of dolichyl phosphate or
            N-acetylglucosaminyl-pyrophosphoryl-dolichol
  JOURNAL   Biochem Biophys Res Commun 200 (2), 816-820 (1994)
   PUBMED   8179616
REFERENCE   6  (bases 1 to 1805)
  AUTHORS   Bossuyt,X. and Blanckaert,N.
  TITLE     Effect of GTP on the dolichol pathway for protein glycosylation in
            rat liver microsomes
  JOURNAL   Biochem J 296 (Pt 3) (Pt 3), 633-637 (1993)
   PUBMED   8280061
REFERENCE   7  (bases 1 to 1805)
  AUTHORS   Rajput,B., Ma,J., Muniappa,N., Schantz,L., Naylor,S.L., Lalley,P.A.
            and Vijay,I.K.
  TITLE     Mouse UDP-GlcNAc: dolichyl-phosphate
            N-acetylglucosaminephosphotransferase. Molecular cloning of the
            cDNA, generation of anti-peptide antibodies and chromosomal
            localization
  JOURNAL   Biochem J 285 (Pt 3) (Pt 3), 985-992 (1992)
   PUBMED   1323278
REFERENCE   8  (bases 1 to 1805)
  AUTHORS   Varki,A., Reitman,M.L., Vannier,A., Kornfeld,S., Grubb,J.H. and
            Sly,W.S.
  TITLE     Demonstration of the heterozygous state for I-cell disease and
            pseudo-Hurler polydystrophy by assay of
            N-acetylglucosaminylphosphotransferase in white blood cells and
            fibroblasts
  JOURNAL   Am J Hum Genet 34 (5), 717-729 (1982)
   PUBMED   6289658
COMMENT     PROVISIONAL REFSEQ: This record has not yet been subject to final
            NCBI review. The reference sequence was derived from
            JAXUCZ010000008.1.
            
            On Nov 27, 2020 this sequence version replaced NM_199388.1.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC063184.1, FQ213783.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA5760383, SAMEA5760386
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-289               JAXUCZ010000008.1  53561216-53561504
            290-410             JAXUCZ010000008.1  53561844-53561964
            411-624             JAXUCZ010000008.1  53562196-53562409
            625-771             JAXUCZ010000008.1  53562901-53563047
            772-856             JAXUCZ010000008.1  53565125-53565209
            857-1045            JAXUCZ010000008.1  53565913-53566101
            1046-1133           JAXUCZ010000008.1  53566607-53566694
            1134-1289           JAXUCZ010000008.1  53566861-53567016
            1290-1805           JAXUCZ010000008.1  53567102-53567617
FEATURES             Location/Qualifiers
     source          1..1805
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="BN"
                     /db_xref="taxon:10116"
                     /chromosome="8"
                     /map="8q22"
     gene            1..1805
                     /gene="Dpagt1"
                     /gene_synonym="H2afx"
                     /note="dolichyl-phosphate
                     N-acetylglucosaminephosphotransferase 1"
                     /db_xref="GeneID:300668"
                     /db_xref="RGD:735124"
     exon            1..289
                     /gene="Dpagt1"
                     /gene_synonym="H2afx"
                     /inference="alignment:Splign:2.1.0"
     CDS             123..1355
                     /gene="Dpagt1"
                     /gene_synonym="H2afx"
                     /EC_number="2.7.8.15"
                     /note="dolichyl-phosphate (UDP-N-acetylglucosamine)
                     acetylglucosaminephosphotransferase 1 (GlcNAc-1-P
                     transferase); dolichyl-phosphate (UDP-N-acetylglucosamine)
                     N-acetylglucosaminephosphotransferase 1 (GlcNAc-1-P
                     transferase)"
                     /codon_start=1
                     /product="UDP-N-acetylglucosamine--dolichyl-phosphate
                     N-acetylglucosaminephosphotransferase"
                     /protein_id="NP_955420.1"
                     /db_xref="GeneID:300668"
                     /db_xref="RGD:735124"
                     /translation="MWAFPELPLPLPLLVNLIGSLLGFVATVTLIPAFRSHFIAARLC
                     GQDLNKLSRQQIPESQGVISGAVFLIILFCFIPFPFLNCFVEEQCKAFPHHEFVALIG
                     ALLAICCMIFLGFADDVLNLRWRHKLLLPTAASLPLLMVYFTNFGNTTIVVPKPFRWI
                     LGLHLDLGILYYVYMGLLAVFCTNAINILAGINGLEAGQSLVISASIIVFNLVELEGD
                     YRDDHVFSLYFMMPFFFTTLGLLYHNWYPSQVFVGDTFCYFAGMTFAVVGILGHFSKT
                     MLLFFMPQVFNFLYSLPQLFQIIPCPRHRMPRLNTKTGKLEMSYSKFKTKSLSFLGTF
                     ILKVAESLRLVTVHRGESEDGAFTECNNMTLINLLLKVFGPTHERNLTLFLLLLQVLS
                     SAVTFSIRYQLVRLFYDV"
     exon            290..410
                     /gene="Dpagt1"
                     /gene_synonym="H2afx"
                     /inference="alignment:Splign:2.1.0"
     exon            411..624
                     /gene="Dpagt1"
                     /gene_synonym="H2afx"
                     /inference="alignment:Splign:2.1.0"
     exon            625..771
                     /gene="Dpagt1"
                     /gene_synonym="H2afx"
                     /inference="alignment:Splign:2.1.0"
     exon            772..856
                     /gene="Dpagt1"
                     /gene_synonym="H2afx"
                     /inference="alignment:Splign:2.1.0"
     exon            857..1045
                     /gene="Dpagt1"
                     /gene_synonym="H2afx"
                     /inference="alignment:Splign:2.1.0"
     exon            1046..1133
                     /gene="Dpagt1"
                     /gene_synonym="H2afx"
                     /inference="alignment:Splign:2.1.0"
     exon            1134..1289
                     /gene="Dpagt1"
                     /gene_synonym="H2afx"
                     /inference="alignment:Splign:2.1.0"
     exon            1290..1805
                     /gene="Dpagt1"
                     /gene_synonym="H2afx"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 gctggcgccg gaatcctctg agtgtaggga gctgcactgc tggctgccga ggcctctggt
       61 ttgttcctgg cttaccaagt tagctgagta ggcggcggag cggcggcccc cggagggtca
      121 ctatgtgggc cttcccggag ttacctctgc cgctgccgct gttggtgaat ttgatcggat
      181 cgctgttggg atttgtggct accgtcaccc tcatccctgc cttccgtagc cactttatcg
      241 ccgcgcgtct ctgtggccag gacctcaaca agctcagccg gcagcagatc ccagagtccc
      301 agggagtgat cagcggtgct gttttcctta tcatcctctt ctgcttcatc cctttccctt
      361 tcctgaactg ctttgtggag gagcagtgta aggcattccc ccaccatgaa tttgtggccc
      421 tgataggtgc cctccttgct atctgctgca tgatcttcct gggttttgct gatgacgtac
      481 tcaatctgcg atggcgtcat aagctgctgc tccccacagc tgcctcacta cctctcctca
      541 tggtctactt cactaacttt ggcaatacaa ccattgtggt gccgaagccc ttccgctgga
      601 ttcttggcct gcatttggat ttgggcatcc tgtactatgt ctacatggga ctgcttgcag
      661 tgttctgtac caatgccatc aacatcctag cgggaattaa tggcctagag gctggtcaat
      721 cactagtcat ctctgcttct attattgtct tcaacctggt ggagctggaa ggtgattatc
      781 gggacgatca tgtcttttcc ctctacttca tgatgccatt tttttttacc accttgggat
      841 tgctgtacca taactggtac ccgtctcagg tgtttgtggg agacaccttc tgttattttg
      901 ctggcatgac ctttgccgtg gtgggaatct tgggacactt cagcaagacc atgctgctct
      961 tctttatgcc acaagtattc aatttcctct actcactgcc tcagctcttt cagatcatcc
     1021 cctgccctcg acaccgtatg cccagactca atacgaagac aggcaaactg gagatgagct
     1081 attccaagtt caagaccaag agcctctctt tcttgggcac gtttatttta aaggtagcag
     1141 agagcctccg gctggtgaca gttcaccgag gggagagtga ggatggtgcc ttcactgagt
     1201 gtaacaacat gaccctcatc aacttgctac ttaaagtctt tgggcctaca catgagagaa
     1261 acctcacact gttcctgctg ctcctgcagg ttctgagcag cgctgtcacc ttctccattc
     1321 gttaccagct cgtccgactc ttctatgatg tctgagctcc ctgacgactg ccctttacca
     1381 cacagtctcc attggacctc agccaggacc cacctctgtc cgctccgacc gccttctggt
     1441 ccaggctcag cttctgccgt catctgtgac tactgacatc ctggatggac tccttagtgg
     1501 acttgacgtc cactagttgg actttgccta tgctttcttg agtttgctac tccctccctt
     1561 tctgcagcct caccaggtgg gcctgtagca tcttttatgc aaatattcat ggctcaactt
     1621 tcagaaccct aactctaaag gaatcccctg ggccttgaga gagaacctgg gctagggcta
     1681 gagttagggc aacatactcc aaggtaacct cacatctgac tatcaaatta agtgttctga
     1741 ttaggaagag cagaggcagg gccatgtgct cagaatggtg acaataaagg attgcctttt
     1801 acttg
//
