LOCUS       NM_001394746            2596 bp    mRNA    linear   ROD 30-MAR-2024
DEFINITION  Rattus norvegicus dematin actin binding protein (Dmtn), transcript
            variant 1, mRNA.
ACCESSION   NM_001394746 XM_017599746
VERSION     NM_001394746.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 2596)
  AUTHORS   Chen,L., Brown,J.W., Mok,Y.F., Hatters,D.M. and McKnight,C.J.
  TITLE     The allosteric mechanism induced by protein kinase A (PKA)
            phosphorylation of dematin (band 4.9)
  JOURNAL   J Biol Chem 288 (12), 8313-8320 (2013)
   PUBMED   23355471
  REMARK    Erratum:[J Biol Chem. 2015 Jul 17;290(29):17808. PMID: 26188045]
REFERENCE   2  (bases 1 to 2596)
  AUTHORS   Wieschhaus,A.J., Le Breton,G.C. and Chishti,A.H.
  TITLE     Headpiece domain of dematin regulates calcium mobilization and
            signaling in platelets
  JOURNAL   J Biol Chem 287 (49), 41218-41231 (2012)
   PUBMED   23060452
REFERENCE   3  (bases 1 to 2596)
  AUTHORS   Koshino,I., Mohandas,N. and Takakuwa,Y.
  TITLE     Identification of a novel role for dematin in regulating red cell
            membrane function by modulating spectrin-actin interaction
  JOURNAL   J Biol Chem 287 (42), 35244-35250 (2012)
   PUBMED   22927433
REFERENCE   4  (bases 1 to 2596)
  AUTHORS   Chen,L., Jiang,Z.G., Khan,A.A., Chishti,A.H. and McKnight,C.J.
  TITLE     Dematin exhibits a natively unfolded core domain and an
            independently folded headpiece domain
  JOURNAL   Protein Sci 18 (3), 629-636 (2009)
   PUBMED   19241372
REFERENCE   5  (bases 1 to 2596)
  AUTHORS   Robledo,R.F., Ciciotte,S.L., Gwynn,B., Sahr,K.E., Gilligan,D.M.,
            Mohandas,N. and Peters,L.L.
  TITLE     Targeted deletion of alpha-adducin results in absent beta- and
            gamma-adducin, compensated hemolytic anemia, and lethal
            hydrocephalus in mice
  JOURNAL   Blood 112 (10), 4298-4307 (2008)
   PUBMED   18723693
REFERENCE   6  (bases 1 to 2596)
  AUTHORS   Mohseni,M. and Chishti,A.H.
  TITLE     The headpiece domain of dematin regulates cell shape, motility, and
            wound healing by modulating RhoA activation
  JOURNAL   Mol Cell Biol 28 (15), 4712-4718 (2008)
   PUBMED   18505823
REFERENCE   7  (bases 1 to 2596)
  AUTHORS   Khan,A.A., Hanada,T., Mohseni,M., Jeong,J.J., Zeng,L., Gaetani,M.,
            Li,D., Reed,B.C., Speicher,D.W. and Chishti,A.H.
  TITLE     Dematin and adducin provide a novel link between the spectrin
            cytoskeleton and human erythrocyte membrane by directly interacting
            with glucose transporter-1
  JOURNAL   J Biol Chem 283 (21), 14600-14609 (2008)
   PUBMED   18347014
REFERENCE   8  (bases 1 to 2596)
  AUTHORS   Munton,R.P., Tweedie-Cullen,R., Livingstone-Zatchej,M.,
            Weinandy,F., Waidelich,M., Longo,D., Gehrig,P., Potthast,F.,
            Rutishauser,D., Gerrits,B., Panse,C., Schlapbach,R. and Mansuy,I.M.
  TITLE     Qualitative and quantitative analyses of protein phosphorylation in
            naive and stimulated mouse synaptosomal preparations
  JOURNAL   Mol Cell Proteomics 6 (2), 283-293 (2007)
   PUBMED   17114649
REFERENCE   9  (bases 1 to 2596)
  AUTHORS   Khanna,R., Chang,S.H., Andrabi,S., Azam,M., Kim,A., Rivera,A.,
            Brugnara,C., Low,P.S., Liu,S.C. and Chishti,A.H.
  TITLE     Headpiece domain of dematin is required for the stability of the
            erythrocyte membrane
  JOURNAL   Proc Natl Acad Sci U S A 99 (10), 6637-6642 (2002)
   PUBMED   12011427
REFERENCE   10 (bases 1 to 2596)
  AUTHORS   Lutchman,M., Pack,S., Kim,A.C., Azim,A., Emmert-Buck,M., van
            Huffel,C., Zhuang,Z. and Chishti,A.H.
  TITLE     Loss of heterozygosity on 8p in prostate cancer implicates a role
            for dematin in tumor progression
  JOURNAL   Cancer Genet Cytogenet 115 (1), 65-69 (1999)
   PUBMED   10565303
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            JAXUCZ010000015.1.
            
            On May 4, 2021 this sequence version replaced XM_017599746.2.
            
            ##Evidence-Data-START##
            RNAseq introns :: partial sample support SAMN12840117, SAMN12840131
                              [ECO:0000350]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            inferred exon combination :: based on alignments, homology
            RefSeq Select criteria    :: based on conservation, expression,
                                         longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-131               JAXUCZ010000015.1  52111826-52111956   c
            132-301             JAXUCZ010000015.1  52103695-52103864   c
            302-376             JAXUCZ010000015.1  52103415-52103489   c
            377-532             JAXUCZ010000015.1  52102728-52102883   c
            533-577             JAXUCZ010000015.1  52101524-52101568   c
            578-677             JAXUCZ010000015.1  52101071-52101170   c
            678-734             JAXUCZ010000015.1  52100678-52100734   c
            735-887             JAXUCZ010000015.1  52100250-52100402   c
            888-1012            JAXUCZ010000015.1  52097542-52097666   c
            1013-1118           JAXUCZ010000015.1  52096500-52096605   c
            1119-1183           JAXUCZ010000015.1  52089956-52090020   c
            1184-1232           JAXUCZ010000015.1  52089750-52089798   c
            1233-1240           JAXUCZ010000015.1  52089597-52089604   c
            1241-1306           JAXUCZ010000015.1  52089349-52089414   c
            1307-1387           JAXUCZ010000015.1  52089008-52089088   c
            1388-2596           JAXUCZ010000015.1  52087667-52088875   c
FEATURES             Location/Qualifiers
     source          1..2596
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="BN"
                     /db_xref="taxon:10116"
                     /chromosome="15"
                     /map="15p11"
     gene            1..2596
                     /gene="Dmtn"
                     /gene_synonym="Epb4.9; Epb49"
                     /note="dematin actin binding protein"
                     /db_xref="GeneID:361069"
                     /db_xref="RGD:1311419"
     exon            1..131
                     /gene="Dmtn"
                     /gene_synonym="Epb4.9; Epb49"
                     /inference="alignment:Splign:2.1.0"
     exon            132..301
                     /gene="Dmtn"
                     /gene_synonym="Epb4.9; Epb49"
                     /inference="alignment:Splign:2.1.0"
     CDS             284..1501
                     /gene="Dmtn"
                     /gene_synonym="Epb4.9; Epb49"
                     /note="isoform 1 is encoded by transcript variant 1;
                     erythrocyte protein band 4.9; erythrocyte membrane protein
                     band 4.9 (dematin)"
                     /codon_start=1
                     /product="dematin isoform 1"
                     /protein_id="NP_001381675.1"
                     /db_xref="GeneID:361069"
                     /db_xref="RGD:1311419"
                     /translation="MERLQKQPLTSPGSVSSSRDSSVPGSPSNIVAKMDNQVLGYKDL
                     AAIPKDKAILDIERPDLMIYEPHFTYSLLEHVELPRSRECSLSPKSTSPPPSPEVWAE
                     SRTLGIISQASTPRTTGTPRTSLPHFHHPETTRPDSNIYKKPPIYKQRESVGGSPQSK
                     HLIEDLIIESSKFPAAQPPDPNQPAKIETDYWPCPPSLAVVETEWRKRKASRKGAEEE
                     EEEEDDDSEEEIKAIRERQKEELSKVTSNLGKMILKEEMEKSLPIRRKTRSLPDRTPF
                     HTSLHSGTSKSSSLPSYGRTTLSRLQSTEFSPSGSEAGSPGLQNGEGQRGRMDRGNSL
                     PCVLEQKIYPYEMLVVTNKGRTKLPPGVDRMRLERHLSAEDFSRVFAMSPEEFGKLAL
                     WKRNELKKKASLF"
     exon            302..376
                     /gene="Dmtn"
                     /gene_synonym="Epb4.9; Epb49"
                     /inference="alignment:Splign:2.1.0"
     exon            377..532
                     /gene="Dmtn"
                     /gene_synonym="Epb4.9; Epb49"
                     /inference="alignment:Splign:2.1.0"
     exon            533..577
                     /gene="Dmtn"
                     /gene_synonym="Epb4.9; Epb49"
                     /inference="alignment:Splign:2.1.0"
     exon            578..677
                     /gene="Dmtn"
                     /gene_synonym="Epb4.9; Epb49"
                     /inference="alignment:Splign:2.1.0"
     exon            678..734
                     /gene="Dmtn"
                     /gene_synonym="Epb4.9; Epb49"
                     /inference="alignment:Splign:2.1.0"
     exon            735..887
                     /gene="Dmtn"
                     /gene_synonym="Epb4.9; Epb49"
                     /inference="alignment:Splign:2.1.0"
     exon            888..1012
                     /gene="Dmtn"
                     /gene_synonym="Epb4.9; Epb49"
                     /inference="alignment:Splign:2.1.0"
     exon            1013..1118
                     /gene="Dmtn"
                     /gene_synonym="Epb4.9; Epb49"
                     /inference="alignment:Splign:2.1.0"
     exon            1119..1183
                     /gene="Dmtn"
                     /gene_synonym="Epb4.9; Epb49"
                     /inference="alignment:Splign:2.1.0"
     exon            1184..1232
                     /gene="Dmtn"
                     /gene_synonym="Epb4.9; Epb49"
                     /inference="alignment:Splign:2.1.0"
     exon            1233..1240
                     /gene="Dmtn"
                     /gene_synonym="Epb4.9; Epb49"
                     /inference="alignment:Splign:2.1.0"
     exon            1241..1306
                     /gene="Dmtn"
                     /gene_synonym="Epb4.9; Epb49"
                     /inference="alignment:Splign:2.1.0"
     exon            1307..1387
                     /gene="Dmtn"
                     /gene_synonym="Epb4.9; Epb49"
                     /inference="alignment:Splign:2.1.0"
     exon            1388..2596
                     /gene="Dmtn"
                     /gene_synonym="Epb4.9; Epb49"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 cgcggctggg ttcctggagg agctgcctct tacctagatt cctgagaaaa cgccgaaaac
       61 ccgtggacca gggcctaggc cctttgcctc ttggggcttc agagagcctg ggccccactg
      121 tctgggagga gccacccgcg gtcaacagcg cagagaggcc caagggatga ggacaccaca
      181 gagcgttgtg aagccctcgc tgcaggagcc gggccgagcc tgacgtctgt cctctccacg
      241 tgcgcaccgg cttccgaggg agcggccggc cagtggctgc cgcatggaac gtctgcagaa
      301 gcaacctctt acctccccgg ggagcgtcag ctcctccaga gactccagtg tacccggctc
      361 tccctccaac atcgtggcca agatggacaa ccaggtgttg ggctacaaag acctggccgc
      421 catccccaag gacaaggcca tcctggacat tgagagacct gacctcatga tctacgagcc
      481 ccattttacc tattccctcc tggaacacgt agagcttccc agaagtcggg agtgctcact
      541 gtcacccaaa tccacatccc caccaccttc tccagaggtg tgggcggaga gccggacact
      601 tggaatcatc tctcaggctt caacaccaag gaccacagga acccccagga ccagcctgcc
      661 ccatttccac caccctgaga ctacccgccc ggattccaac atctacaaga agccacccat
      721 ctacaagcag agagaatcgg tgggaggcag ccctcagagc aagcacctca tcgaggacct
      781 catcattgag tcatccaagt tcccagcagc acagccccct gatcccaacc agccagccaa
      841 gatagagact gactattggc cgtgtccccc gtcgctggct gttgtggaga cagaatggag
      901 gaagcggaag gcatctcgaa agggcgcaga ggaagaggag gaggaggaag atgatgactc
      961 tgaagaggag attaaggcca tcagggagcg gcagaaagag gaactcagta aggttacttc
     1021 caacttggga aagatgatct tgaaagaaga gatggaaaag tcattgccca tccggaggaa
     1081 aacgcgctct ctgcctgacc ggacaccctt ccatacctcc ttgcattcgg gaacgtctaa
     1141 gtcctcgtcc cttccttcct atggcaggac caccctgagt cggctacagt ccacagaatt
     1201 cagcccatcc ggaagtgagg ctgggagccc aggcctgcag aacggagagg gccagagggg
     1261 gaggatggat cgggggaact ccctgccctg tgtgctggag cagaagatct atccctatga
     1321 gatgctggtg gtgaccaaca aggggagaac taagctgcct cccggtgtgg accgcatgag
     1381 gcttgagagg catttgtcag cagaggactt ctctagggtc ttcgccatgt ctcctgagga
     1441 gtttggcaag ctggccctgt ggaagcggaa cgaactcaag aagaaagctt ccctcttctg
     1501 acatccctgc cagctcgccc tgtccccgtg acagtacctt ccttctgctg cttgaggccc
     1561 tgaagctggg gctgtgagac cccacagtga ctcctcttcc tctgctggct gggagcagca
     1621 ggaggctggg cctaagtgtc aggctccact cctcagggag agagccaaga agtgcacagt
     1681 cccagagtca tgaggcatgg acttccttcc ccacgatggg ccagggggcc ttaccttttc
     1741 cctgggctcc actgcctagc caaacccagt ccagactcca gcacacagtc aatgtttgca
     1801 cccatttcct gtctgtcacc ccacatgagg gtccccagga gacagtgagg agaaatgaag
     1861 ggagtttagt ggagaggtct gtctgctatc ctgtacaggg ggaggcagca gctctgggac
     1921 cctgtgcctg tctacccaaa ggctccagcc agttgcactc ctgacagctt cctgcactca
     1981 ggcccgatgc ttctctaact ggcagaagca ggctctacct gggcctccag gtgggaggtc
     2041 atcctccctc tagcttatat gtcctgttct ttgcaacccc taagcttcaa acgtcttgct
     2101 cgagaccaga gctcattcaa agctctggct aagagcagag acgctattca cacgacgcct
     2161 caccaccacc agcctccagg caaaaccaga tagactgcac ccttctatca cctgtccagg
     2221 gaccacagag cccaggcctc agcctccctt acatgggact aagagaccta gcttggggca
     2281 tctctgagag ttcaggctcc caggtccctc ctccgtttgc tgtgcatctc ctactgtgct
     2341 ctctacagaa tgtaatttat tggggcaccc tctgctgctt cctttccctg actccttgcc
     2401 ctacctaatt gcacttccag tcctgtctgt ccacatatgt acatgtatat aattatacat
     2461 atatatatat atatctattt tgcccagatc tgggtttcgt ttttacccag accccggcac
     2521 tccctgtcca ctgtgtgtga gatcatacta ggaggggtaa ctgcttggaa ttaaaaggtt
     2581 gcactgggtc cctaaa
//
