LOCUS       NM_139102               3011 bp    mRNA    linear   ROD 22-MAR-2023
DEFINITION  Rattus norvegicus dimethylglycine dehydrogenase (Dmgdh), mRNA;
            nuclear gene for mitochondrial product.
ACCESSION   NM_139102 XM_346609
VERSION     NM_139102.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 3011)
  AUTHORS   Cao Y, Ding W, Zhang J, Gao Q, Yang H, Cao W, Wang Z, Fang L and Du
            R.
  TITLE     Significant Down-Regulation of Urea Cycle Generates Clinically
            Relevant Proteomic Signature in Hepatocellular Carcinoma Patients
            with Macrovascular Invasion
  JOURNAL   J Proteome Res 18 (5), 2032-2044 (2019)
   PUBMED   30901224
REFERENCE   2  (bases 1 to 3011)
  AUTHORS   Luka Z, Pakhomova S, Loukachevitch LV, Newcomer ME and Wagner C.
  TITLE     Folate in demethylation: the crystal structure of the rat
            dimethylglycine dehydrogenase complexed with tetrahydrofolate
  JOURNAL   Biochem Biophys Res Commun 449 (4), 392-398 (2014)
   PUBMED   24858690
  REMARK    GeneRIF: crystal structures of mature and precursor forms of DMGDH
            in presence of tetrahydrofolate; folate binding site is connected
            with enzyme active center via an intramolecular channel suggesting
            possible transfer of the intermediate imine of dimethylglycine from
            the active center to the bound THF where they could react producing
            a 5,10-methylenetetrahydrofolate
REFERENCE   3  (bases 1 to 3011)
  AUTHORS   Castello A, Fischer B, Eichelbaum K, Horos R, Beckmann BM, Strein
            C, Davey NE, Humphreys DT, Preiss T, Steinmetz LM, Krijgsveld J and
            Hentze MW.
  TITLE     Insights into RNA biology from an atlas of mammalian mRNA-binding
            proteins
  JOURNAL   Cell 149 (6), 1393-1406 (2012)
   PUBMED   22658674
REFERENCE   4  (bases 1 to 3011)
  AUTHORS   Tibbetts AS and Appling DR.
  TITLE     Compartmentalization of Mammalian folate-mediated one-carbon
            metabolism
  JOURNAL   Annu Rev Nutr 30, 57-81 (2010)
   PUBMED   20645850
  REMARK    Review article
REFERENCE   5  (bases 1 to 3011)
  AUTHORS   Brizio C, Brandsch R, Douka M, Wait R and Barile M.
  TITLE     The purified recombinant precursor of rat mitochondrial
            dimethylglycine dehydrogenase binds FAD via an autocatalytic
            reaction
  JOURNAL   Int J Biol Macromol 42 (5), 455-462 (2008)
   PUBMED   18423846
  REMARK    GeneRIF: covalent attachment of FAD to dimethylglycine
            dehydrogenase apoenzyme can proceed in vitro autocatalytically
REFERENCE   6  (bases 1 to 3011)
  AUTHORS   Lang H, Minaian K, Freudenberg N, Hoffmann R and Brandsch R.
  TITLE     Tissue specificity of rat mitochondrial dimethylglycine
            dehydrogenase expression
  JOURNAL   Biochem J 299 (Pt 2) (Pt 2), 393-398 (1994)
   PUBMED   7513513
REFERENCE   7  (bases 1 to 3011)
  AUTHORS   Lang H, Polster M and Brandsch R.
  TITLE     Rat liver dimethylglycine dehydrogenase. Flavinylation of the
            enzyme in hepatocytes in primary culture and characterization of a
            cDNA clone
  JOURNAL   Eur J Biochem 198 (3), 793-799 (1991)
   PUBMED   1710985
REFERENCE   8  (bases 1 to 3011)
  AUTHORS   Cook,R.J., Misono,K.S. and Wagner,C.
  TITLE     The amino acid sequences of the flavin-peptides of dimethylglycine
            dehydrogenase and sarcosine dehydrogenase from rat liver
            mitochondria
  JOURNAL   J Biol Chem 260 (24), 12998-13002 (1985)
   PUBMED   4055729
REFERENCE   9  (bases 1 to 3011)
  AUTHORS   Wittwer,A.J. and Wagner,C.
  TITLE     Identification of the folate-binding proteins of rat liver
            mitochondria as dimethylglycine dehydrogenase and sarcosine
            dehydrogenase. Flavoprotein nature and enzymatic properties of the
            purified proteins
  JOURNAL   J Biol Chem 256 (8), 4109-4115 (1981)
   PUBMED   6163778
REFERENCE   10 (bases 1 to 3011)
  AUTHORS   Wittwer,A.J. and Wagner,C.
  TITLE     Identification of folate binding protein of mitochondria as
            dimethylglycine dehydrogenase
  JOURNAL   Proc Natl Acad Sci U S A 77 (8), 4484-4488 (1980)
   PUBMED   6159630
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            BC085697.1.
            
            On or before Jan 12, 2005 this sequence version replaced
            XM_346609.1, NM_139102.1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC085697.1, X55995.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA5756307, SAMEA5760383
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            gene product(s) localized to mito. :: inferred from homology
            RefSeq Select criteria             :: based on conservation,
                                                  expression
            ##RefSeq-Attributes-END##
FEATURES             Location/Qualifiers
     source          1..3011
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /db_xref="taxon:10116"
                     /chromosome="2"
                     /map="2q12"
     gene            1..3011
                     /gene="Dmgdh"
                     /gene_synonym="Me2GlyDH"
                     /note="dimethylglycine dehydrogenase"
                     /db_xref="GeneID:245961"
                     /db_xref="RGD:620453"
     exon            1..118
                     /gene="Dmgdh"
                     /gene_synonym="Me2GlyDH"
                     /inference="alignment:Splign:2.1.0"
     CDS             33..2606
                     /gene="Dmgdh"
                     /gene_synonym="Me2GlyDH"
                     /EC_number="1.5.8.4"
                     /note="dimethylglycine dehydrogenase, mitochondrial"
                     /codon_start=1
                     /product="dimethylglycine dehydrogenase, mitochondrial
                     precursor"
                     /protein_id="NP_620802.2"
                     /db_xref="GeneID:245961"
                     /db_xref="RGD:620453"
                     /translation="MLRLGALRLRGLALRSSQGRPSSAGLREGQESPPSPPEWKDRAE
                     TVIIGGGCVGVSLAYHLAKAGMRDVVLLEKSELTAGSTWHAAGLTTYFHPGINLKKIH
                     YDSIKLYERLEEETGQVVGFHQPGSIRLATTPERVDEFKYQMTRTNWHATEQYIIEPE
                     KIHELFPLLNMDKILAGLYNPGDGHIDPYSLTMALATGARKYGALLKYPAPVTSLKPR
                     PDGTWDVETPQGSVRANRIVNAAGFWAREVGKMIGLDHPLIPVQHQYVITSTIPEVKA
                     LKRELPVLRDLEGSYYLRQERDGLLFGPYESQEKMKLQASWVAHGVPPGFGKELFESD
                     LDRITEHVEAAMEMVPVLKKADIINIVNGPITYSPDILPMVGPHQGVRNYWVAIGFGY
                     GIIHAGGVGTYLSDWILHGEPPFDLIELDPNRYGKWTTTQYTEAKARESYGFNNIVGY
                     PKEERFAGRPTQRVSGLYKILESKCSMGFHAGWEQPHWFYKPGQDTQYRPSFRRTNWF
                     EPVGSEYKQVMQRVGVIDLSPFGKFNIKGQDSTQLLDHLCANVIPKVGFTNISHMLTP
                     RGRVYAELTVSHQSPGEFLLITGSGSELHDLRWIEEAAVRGGYDVEIRNITDELGVLG
                     VAGPYARRVLQKLTSEDLSDDVFKFLQTKSLKISDIPVTAIRISYTGELGWELYHRRE
                     DSAALYERIMNAGQEEGIDNFGTYALNALRLEKAFRAWGSEMNCDTNPLEAGLDYFIK
                     LNKPANFTGKQALKQIKAKGLKRRLVCLTLATDNVDPEGNESVWYKGKVIGNTTSGSY
                     SYSIQKSLAFAYVPVELSEVGQQVEVELLGKNYPATIIQEPLVLTEPTRTRLQKDGRK
                     S"
     exon            119..287
                     /gene="Dmgdh"
                     /gene_synonym="Me2GlyDH"
                     /inference="alignment:Splign:2.1.0"
     exon            288..386
                     /gene="Dmgdh"
                     /gene_synonym="Me2GlyDH"
                     /inference="alignment:Splign:2.1.0"
     exon            387..551
                     /gene="Dmgdh"
                     /gene_synonym="Me2GlyDH"
                     /inference="alignment:Splign:2.1.0"
     exon            552..756
                     /gene="Dmgdh"
                     /gene_synonym="Me2GlyDH"
                     /inference="alignment:Splign:2.1.0"
     exon            757..1005
                     /gene="Dmgdh"
                     /gene_synonym="Me2GlyDH"
                     /inference="alignment:Splign:2.1.0"
     exon            1006..1204
                     /gene="Dmgdh"
                     /gene_synonym="Me2GlyDH"
                     /inference="alignment:Splign:2.1.0"
     exon            1205..1374
                     /gene="Dmgdh"
                     /gene_synonym="Me2GlyDH"
                     /inference="alignment:Splign:2.1.0"
     exon            1375..1528
                     /gene="Dmgdh"
                     /gene_synonym="Me2GlyDH"
                     /inference="alignment:Splign:2.1.0"
     exon            1529..1694
                     /gene="Dmgdh"
                     /gene_synonym="Me2GlyDH"
                     /inference="alignment:Splign:2.1.0"
     exon            1695..1825
                     /gene="Dmgdh"
                     /gene_synonym="Me2GlyDH"
                     /inference="alignment:Splign:2.1.0"
     exon            1826..2043
                     /gene="Dmgdh"
                     /gene_synonym="Me2GlyDH"
                     /inference="alignment:Splign:2.1.0"
     exon            2044..2201
                     /gene="Dmgdh"
                     /gene_synonym="Me2GlyDH"
                     /inference="alignment:Splign:2.1.0"
     exon            2202..2261
                     /gene="Dmgdh"
                     /gene_synonym="Me2GlyDH"
                     /inference="alignment:Splign:2.1.0"
     exon            2262..2396
                     /gene="Dmgdh"
                     /gene_synonym="Me2GlyDH"
                     /inference="alignment:Splign:2.1.0"
     exon            2397..2985
                     /gene="Dmgdh"
                     /gene_synonym="Me2GlyDH"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 gggatctgtt tgtgctcggc tgcaaccttg ccatgctccg cctgggcgcg ctcaggctgc
       61 ggggcctcgc gctccggagc tcccaggggc gcccaagctc cgccggcctc cgggaaggac
      121 aggaaagccc accctcaccc ccagaatgga aggacagagc agagactgtg atcattggag
      181 gtggctgcgt gggcgtgagt ctggcttacc atctggccaa ggcagggatg agagacgtgg
      241 tcctgttgga gaagtcggaa ctcacggctg gctctacctg gcacgcagca ggtttaacaa
      301 cttactttca ccccgggata aacttgaaga agatacatta tgacagcatc aaactttacg
      361 agaggctgga agaagaaact ggacaggtgg tggggttcca tcaaccgggt agtatcaggc
      421 ttgcgacaac tcctgaaagg gtggacgaat ttaaatacca aatgacgcga accaactggc
      481 atgccaccga gcagtatatt attgagcccg aaaaaatcca cgagctgttc cctttactca
      541 acatggacaa gattttagcc ggactgtata acccgggaga tggccacatc gatccatatt
      601 ctctgacgat ggccctggct actggagcta ggaaatatgg ggcccttcta aagtatcctg
      661 caccagtgac atctctgaag cccaggccag atgggacatg ggacgtggag actccgcaag
      721 gatctgtgag agcaaatcga attgtgaatg ctgcgggatt ttgggctcgt gaagtgggta
      781 aaatgattgg actagaccat cccctcatcc ctgtgcaaca tcaatatgtc atcacatcaa
      841 caatcccaga agtgaaggct ttgaaacgag aactcccagt ccttcgtgac ttggaaggat
      901 cctactacct ccgacaggaa agggacgggc ttttgtttgg tccctatgaa agtcaggaga
      961 agatgaaact tcaagcctcc tgggtcgctc atggagtccc tccaggcttt gggaaggaac
     1021 tttttgagtc tgacttagac cgaatcacag agcacgtgga agctgccatg gaaatggttc
     1081 ctgttctgaa aaaggcggac atcatcaaca tcgtcaacgg tcctatcacc tactctcctg
     1141 acattctgcc tatggtgggg ccccatcagg gggtcaggaa ctactgggtg gccattggct
     1201 ttggatatgg cataatccac gccggtgggg tcgggacgta cctcagcgac tggatcctgc
     1261 acggagaacc tccctttgac ctgatagaac tggatcccaa ccgctacggc aagtggacga
     1321 cgacgcagta caccgaggcc aaagcccgag aatcatacgg gttcaacaac attgttggtt
     1381 atcctaaaga ggaacggttt gctgggaggc caacccagag agtcagtggg ctctacaaaa
     1441 tcctggagtc caagtgttcc atgggtttcc atgcaggctg ggaacagcca cactggttct
     1501 acaagcccgg ccaggacact cagtataggc caagtttccg ccgcacaaac tggtttgagc
     1561 cggtgggctc tgagtacaaa caggttatgc aaagagttgg tgtgattgac ctgtctccgt
     1621 ttggcaagtt caacatcaaa ggccaagatt ccacccagct tctggatcat ctctgtgcaa
     1681 atgtcattcc caaggtgggt tttacaaaca taagtcacat gttgacgccc agaggtcgag
     1741 tgtacgctga gctgactgtt tcccaccaat ctcctgggga gttcctgttg attactggct
     1801 ctgggtcgga acttcacgat cttagatgga ttgaagaagc agccgtcaga ggtggctatg
     1861 atgtggaaat tcgaaacata accgatgagc ttggggtcct gggagtagca gggccatatg
     1921 caaggagagt ccttcagaag ctgacctcag aagatctcag tgatgatgtc ttcaagtttc
     1981 ttcaaaccaa gtctttaaag atctctgaca ttcctgtcac tgctatcagg atctcctata
     2041 ctggtgagct gggatgggag ctgtaccaca gacgagaaga ttctgcagcc ctttatgagc
     2101 ggatcatgaa tgcgggccag gaggagggca tcgacaattt tggaacatat gccctgaatg
     2161 ccttaagact ggagaaggct ttccgggcct gggggtcaga gatgaactgt gacacaaacc
     2221 ctttggaagc tgggctggac tatttcatca agctgaacaa gccagccaac ttcacaggga
     2281 agcaagcgct gaagcagatt aaagccaagg ggctgaagcg gagactggtg tgcctcacct
     2341 tggcaacaga caatgtggat ccagagggaa atgaaagcgt ttggtacaag ggcaaggtga
     2401 ttggcaacac aacatctgga agctacagtt acagcatcca gaagagcctg gcttttgctt
     2461 atgtcccagt tgagctcagt gaagtgggac aacaagtgga ggttgaacta ctgggtaaaa
     2521 attacccagc aaccattata caggaaccat tggtactcac agaacctacc agaaccaggc
     2581 ttcagaaaga tggtaggaaa tcttgagaaa gaccaacaac cgaggtgtag ctggcagaac
     2641 tgctctggca tcctgctcct tccaggataa agatagccaa gacgtcactt cagaatcacc
     2701 aaaatctaga tcagtatctt cgtaaaaacc ttttcattgt ctcccacaca ccagggtttg
     2761 atgaattaat gatttgcact gtccattcaa aggactttaa gatgaacact ttttattaat
     2821 tttattactc tttattactg gtgcttatga aacccaatca gagaaaacac tgggcgtttg
     2881 ccaatgtgaa atcaggattc tagtgggatt aaaggactgt gattctagga acacagaatg
     2941 tggctgacag ctgagaacaa atgaataaat tgtctttcac agaaaaaaaa aaaaaaaaaa
     3001 aaaaaaaaaa a
//
