LOCUS       NM_001006981            2797 bp    mRNA    linear   ROD 09-FEB-2025
DEFINITION  Rattus norvegicus dihydrolipoamide S-succinyltransferase (Dlst),
            mRNA; nuclear gene for mitochondrial product.
ACCESSION   NM_001006981 XM_216753
VERSION     NM_001006981.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 2797)
  AUTHORS   Remacha,L., Pirman,D., Mahoney,C.E., Coloma,J., Calsina,B.,
            Curras-Freixes,M., Leton,R., Torres-Perez,R., Richter,S., Pita,G.,
            Herraez,B., Cianchetta,G., Honrado,E., Maestre,L., Urioste,M.,
            Aller,J., Garcia-Uriarte,O., Galvez,M.A., Luque,R.M., Lahera,M.,
            Moreno-Rengel,C., Eisenhofer,G., Montero-Conde,C.,
            Rodriguez-Antona,C., Llorca,O., Smolen,G.A., Robledo,M. and
            Cascon,A.
  TITLE     Recurrent Germline DLST Mutations in Individuals with Multiple
            Pheochromocytomas and Paragangliomas
  JOURNAL   Am J Hum Genet 104 (4), 651-664 (2019)
   PUBMED   30929736
  REMARK    Erratum:[Am J Hum Genet. 2019 May 2;104(5):1008-1010. doi:
            10.1016/j.ajhg.2019.04.010.. PMID: 31051110]
REFERENCE   2  (bases 1 to 2797)
  AUTHORS   Wang,Y., Guo,Y.R., Liu,K., Yin,Z., Liu,R., Xia,Y., Tan,L., Yang,P.,
            Lee,J.H., Li,X.J., Hawke,D., Zheng,Y., Qian,X., Lyu,J., He,J.,
            Xing,D., Tao,Y.J. and Lu,Z.
  TITLE     KAT2A coupled with the alpha-KGDH complex acts as a histone H3
            succinyltransferase
  JOURNAL   Nature 552 (7684), 273-277 (2017)
   PUBMED   29211711
REFERENCE   3  (bases 1 to 2797)
  AUTHORS   de Mateo,S., Castillo,J., Estanyol,J.M., Ballesca,J.L. and Oliva,R.
  TITLE     Proteomic characterization of the human sperm nucleus
  JOURNAL   Proteomics 11 (13), 2714-2726 (2011)
   PUBMED   21630459
REFERENCE   4  (bases 1 to 2797)
  AUTHORS   Zhao,X., Leon,I.R., Bak,S., Mogensen,M., Wrzesinski,K., Hojlund,K.
            and Jensen,O.N.
  TITLE     Phosphoproteome analysis of functional mitochondria isolated from
            resting human muscle reveals extensive phosphorylation of inner
            membrane protein complexes and enzymes
  JOURNAL   Mol Cell Proteomics 10 (1), M110.000299 (2011)
   PUBMED   20833797
REFERENCE   5  (bases 1 to 2797)
  AUTHORS   Ghosh,D., Lippert,D., Krokhin,O., Cortens,J.P. and Wilkins,J.A.
  TITLE     Defining the membrane proteome of NK cells
  JOURNAL   J Mass Spectrom 45 (1), 1-25 (2010)
   PUBMED   19946888
REFERENCE   6  (bases 1 to 2797)
  AUTHORS   Sheu,K.F. and Blass,J.P.
  TITLE     The alpha-ketoglutarate dehydrogenase complex
  JOURNAL   Ann N Y Acad Sci 893, 61-78 (1999)
   PUBMED   10672230
  REMARK    Review article
REFERENCE   7  (bases 1 to 2797)
  AUTHORS   Bruschi,S.A., Lindsay,J.G. and Crabb,J.W.
  TITLE     Mitochondrial stress protein recognition of inactivated
            dehydrogenases during mammalian cell death
  JOURNAL   Proc Natl Acad Sci U S A 95 (23), 13413-13418 (1998)
   PUBMED   9811814
REFERENCE   8  (bases 1 to 2797)
  AUTHORS   Matuda,S., Kodama,J., Goshi,N., Takase,C., Nakano,K., Nakagawa,S.
            and Ohta,S.
  TITLE     A polypeptide derived from mitochondrial dihydrolipoamide
            succinyltransferase is located on the plasma membrane in skeletal
            muscle
  JOURNAL   Biochem Biophys Res Commun 241 (1), 151-156 (1997)
   PUBMED   9405249
REFERENCE   9  (bases 1 to 2797)
  AUTHORS   Nakano,K., Matuda,S., Yamanaka,T., Tsubouchi,H., Nakagawa,S.,
            Titani,K., Ohta,S. and Miyata,T.
  TITLE     Purification and molecular cloning of succinyltransferase of the
            rat alpha-ketoglutarate dehydrogenase complex. Absence of a
            sequence motif of the putative E3 and/or E1 binding site
  JOURNAL   J Biol Chem 266 (28), 19013-19017 (1991)
   PUBMED   1918017
REFERENCE   10 (bases 1 to 2797)
  AUTHORS   Ono,K., Hakozaki,M., Kimura,A. and Kochi,H.
  TITLE     Purification, resolution, and reconstitution of rat liver
            branched-chain alpha-keto acid dehydrogenase complex
  JOURNAL   J Biochem 101 (1), 19-27 (1987)
   PUBMED   3571202
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            CB807447.1, BC083858.1, CK355938.1 and CK845707.1.
            
            On Aug 6, 2008 this sequence version replaced NM_001006981.1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC083858.1, D90401.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMD00132261, SAMD00132262
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            gene product(s) localized to mito. :: inferred from homology
            RefSeq Select criteria             :: based on conservation,
                                                  expression
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-14                CB807447.1         1-14
            15-532              BC083858.1         1-518
            533-718             CK355938.1         536-721
            719-2760            BC083858.1         705-2746
            2761-2797           CK845707.1         1-37                c
FEATURES             Location/Qualifiers
     source          1..2797
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="Sprague-Dawley"
                     /db_xref="taxon:10116"
                     /chromosome="6"
                     /map="6q31"
     gene            1..2797
                     /gene="Dlst"
                     /note="dihydrolipoamide S-succinyltransferase"
                     /db_xref="GeneID:299201"
                     /db_xref="RGD:1359615"
     exon            1..106
                     /gene="Dlst"
                     /inference="alignment:Splign:2.1.0"
     CDS             44..1408
                     /gene="Dlst"
                     /EC_number="2.3.1.61"
                     /note="E2; E2K; dihydrolipoamide succinyltransferase
                     component of 2-oxoglutarate dehydrogenase complex;
                     2-oxoglutarate dehydrogenase complex component E2;
                     dihydrolipoamide S-succinyltransferase (E2 component of
                     2-oxo-glutarate complex); OGDC-E2"
                     /codon_start=1
                     /product="dihydrolipoyllysine-residue succinyltransferase
                     component of 2-oxoglutarate dehydrogenase complex,
                     mitochondrial"
                     /protein_id="NP_001006982.2"
                     /db_xref="GeneID:299201"
                     /db_xref="RGD:1359615"
                     /translation="MLSRSRCVSRAFSRSLSAFQKGNCPLGRRSLPGVSLCQGPGYPD
                     SRKMVINNSSVFSVRFFQTTAVCKNDVITVQTPAFAESVTEGDVRWEKAVGDAVAEDE
                     VVCEIETDKTSVQVPSPANGIIEALLVPDGGKVEGGTPLFTLRKTGAAPAKAKPAEAP
                     ATAHKAAPEAPAAPPPPVAPVPTQMPPVPSPSQPPSSKPVSAIKPTAAPPLAEAGAAK
                     GLRSEHREKMNRMRQRIAQRLKEAQNTCAMLTTFNEVDMSNIQEMRARHKDAFLKKHN
                     LKLGFMSAFVKASAFALQEQPVVNAVIDDATKEVVYRDYIDISVAVATPRGLVVPVIR
                     NVETMNYADIERTINELGEKARKNELAIEDMDGGTFTISNGGVFGSLFGTPIINPPQS
                     AILGMHGIFDRPVAVGGKVEVRPMMYVALTYDHRLIDGREAVTFLRKIKAAVEDPRVL
                     LLDL"
     exon            107..140
                     /gene="Dlst"
                     /inference="alignment:Splign:2.1.0"
     exon            141..189
                     /gene="Dlst"
                     /inference="alignment:Splign:2.1.0"
     exon            190..245
                     /gene="Dlst"
                     /inference="alignment:Splign:2.1.0"
     exon            246..320
                     /gene="Dlst"
                     /inference="alignment:Splign:2.1.0"
     exon            321..376
                     /gene="Dlst"
                     /inference="alignment:Splign:2.1.0"
     exon            377..488
                     /gene="Dlst"
                     /inference="alignment:Splign:2.1.0"
     exon            489..641
                     /gene="Dlst"
                     /inference="alignment:Splign:2.1.0"
     exon            642..718
                     /gene="Dlst"
                     /inference="alignment:Splign:2.1.0"
     exon            719..816
                     /gene="Dlst"
                     /inference="alignment:Splign:2.1.0"
     exon            817..947
                     /gene="Dlst"
                     /inference="alignment:Splign:2.1.0"
     exon            948..1021
                     /gene="Dlst"
                     /inference="alignment:Splign:2.1.0"
     exon            1022..1105
                     /gene="Dlst"
                     /inference="alignment:Splign:2.1.0"
     exon            1106..1273
                     /gene="Dlst"
                     /inference="alignment:Splign:2.1.0"
     exon            1274..2779
                     /gene="Dlst"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 ctatatccgg tgtccgcccg ccctccatcc cgctggctcc gcgatgctgt cccggtcccg
       61 ctgcgtgtcc cgggcgttca gccgctcgct ttctgccttc cagaagggga actgccctct
      121 agggagacgt tccctgcctg gggtctcctt atgtcaggga ccaggttacc ctgacagcag
      181 gaagatggtc attaacaaca gtagcgtctt cagtgttcgc ttcttccaaa ccacggcagt
      241 gtgcaagaat gatgtgatta cagtccagac cccagcgttt gcagagtctg tcacagaggg
      301 agatgtcagg tgggagaaag ctgttggaga tgcagttgca gaagatgaag tggtgtgtga
      361 gattgagaca gacaagactt ctgtgcaggt tccatcacca gcaaatggca tcattgaagc
      421 tcttttggta cccgatgggg gcaaagttga aggaggaact cctctattca cactcaggaa
      481 aaccggtgct gctcctgcta aggccaaacc agctgaagcc cctgctacag cccacaaagc
      541 agcgcctgaa gcaccggcgg cccctcctcc tcctgtagca ccagtgccca ctcagatgcc
      601 acctgtgccc tcaccctcac aacctccttc tagcaaacca gtgtctgcaa taaaacccac
      661 tgctgcccct ccactggctg aggcgggagc tgctaaaggc ctgcgctcag aacatcggga
      721 aaagatgaac aggatgcggc agcgcatcgc ccagcgtctg aaggaagccc agaacacctg
      781 cgcaatgctg acgacgttca atgaggttga catgagtaac atacaagaga tgagagctcg
      841 gcacaaagat gctttcctga aaaaacataa cctgaaatta ggcttcatgt cggcatttgt
      901 gaaggcctca gcattcgcct tgcaggagca gcctgtagta aacgcagtga ttgatgacgc
      961 aaccaaggag gtggtgtaca gagattatat tgacatcagt gtcgcagttg ctaccccaag
     1021 gggtctcgtg gttcctgtca tcaggaatgt ggaaactatg aattatgcag atattgaacg
     1081 gaccattaat gaactaggag agaaggcccg gaagaatgaa cttgccatcg aagacatgga
     1141 tgggggcacc ttcaccatca gcaatggagg agttttcggc tcacttttcg gaacacccat
     1201 tatcaacccg cctcagtctg ccattctggg catgcatggc atcttcgaca ggcctgtggc
     1261 tgtggggggc aaggtggaag tccgacctat gatgtatgta gccctgacct acgaccaccg
     1321 gctgattgat ggcagagagg ctgtgacttt cctccgaaaa atcaaggcag cagtagaaga
     1381 tccacgagtc ctcctcctag acctttagga ggaagccaca caccctacct actgatcatg
     1441 caggaactga aaaaccagtc ttctccccga ccctcctcag tcccaggtta acctgagggc
     1501 aggcaggcac taccattggc ctcgagcaag gactccaggg cactacgtaa ccagctttca
     1561 tgggtctttt ttagtgttcc ttctaggctc ttcctctctc cgtgtgactc ttaccttctg
     1621 ctctcttggg cctgagcgag agagagagag agagagagag agagagagag agagagagcc
     1681 ctgtggatgc ccatcaatag tcctgccttt attccagtcc tctacaaaga cacctttgct
     1741 tctctccagt aacagtatgc tatagggata ccaccaggga ccatgtgatt gagtttctgt
     1801 ctttggaaag tgttctccaa agatgcctag ggggaagctt tgcctccggg gctcaaagca
     1861 gcctctgtcg cagctgttct tggtctcact tgatgccccc tgtgtggggt attgaacata
     1921 ggcaaagcag ggcaccctca tcctaacctg tcagcggctt caaggtgcct ctctacctct
     1981 gacaggaagc acaggctgga ggatttaggg ataggcacaa ttgtgctggg tccctgaggc
     2041 tacctgttgt agtcacagat gggagttgaa acttggcttg cctttacagg ctccattgta
     2101 tgctggcact gggaagattc atgttgtcat aactacgtac aactgttgtc catcatacca
     2161 accctttcca gcttctagga atcattctag gacggtgttc tcacgtaaag cagattttag
     2221 catatcatgg cagtgggaca gcctggtgaa cggactgttc tcaccagatc aggctgatga
     2281 gagcttttaa attcaaggtg gtacgagcat gtgctgggaa gggctgttct ctcccttcct
     2341 gggatgtggc ctgtggggag caaggccttg gttcctctgg tgaaggacac agataagggg
     2401 aggatggact gtggcgggaa ccagcctcag gagttaggag agtgaagaga taatgacgtc
     2461 atcgctacac tcacccttca ggggtaggtg gtagttagaa tatttttgaa gcatggtgaa
     2521 atgtaattaa ctggttatga aagctcctag cccttgtgtc cttcactgag cctgagactg
     2581 ctgacagctg acatggggcc attagtgctg aatgcccttt tttcttgtaa tccaagactt
     2641 gctgggggga ggggaagagg tcagtattga gtgcctgcaa catctgctac tatagctata
     2701 ccattcagaa cttgtaatga aaatatttaa agagactgat tttgaatgaa aattaaaggg
     2761 caaatgttct caaacaggga aaaaaaaaaa aaaaaaa
//
