LOCUS       NM_001370997            8079 bp    mRNA    linear   ROD 29-MAR-2024
DEFINITION  Rattus norvegicus DLC1 Rho GTPase activating protein (Dlc1),
            transcript variant 1, mRNA.
ACCESSION   NM_001370997 XM_017600235
VERSION     NM_001370997.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 8079)
  AUTHORS   Bregeon,J., Loirand,G., Pacaud,P. and Rolli-Derkinderen,M.
  TITLE     Angiotensin II induces RhoA activation through SHP2-dependent
            dephosphorylation of the RhoGAP p190A in vascular smooth muscle
            cells
  JOURNAL   Am J Physiol Cell Physiol 297 (5), C1062-C1070 (2009)
   PUBMED   19692654
  REMARK    GeneRIF: Our work then defines RhoGAP p190A as a new mediator of
            RhoA activation by ANG II in vascular smooth muscle cells.
REFERENCE   2  (bases 1 to 8079)
  AUTHORS   Zhong,D., Zhang,J., Yang,S., Soh,U.J., Buschdorf,J.P., Zhou,Y.T.,
            Yang,D. and Low,B.C.
  TITLE     The SAM domain of the RhoGAP DLC1 binds EF1A1 to regulate cell
            migration
  JOURNAL   J Cell Sci 122 (Pt 3), 414-424 (2009)
   PUBMED   19158340
REFERENCE   3  (bases 1 to 8079)
  AUTHORS   Healy,K.D., Hodgson,L., Kim,T.Y., Shutes,A., Maddileti,S.,
            Juliano,R.L., Hahn,K.M., Harden,T.K., Bang,Y.J. and Der,C.J.
  TITLE     DLC-1 suppresses non-small cell lung cancer growth and invasion by
            RhoGAP-dependent and independent mechanisms
  JOURNAL   Mol Carcinog 47 (5), 326-337 (2008)
   PUBMED   17932950
REFERENCE   4  (bases 1 to 8079)
  AUTHORS   Yamaga,M., Kawai,K., Kiyota,M., Homma,Y. and Yagisawa,H.
  TITLE     Recruitment and activation of phospholipase C (PLC)-delta1 in lipid
            rafts by muscarinic stimulation of PC12 cells: contribution of
            p122RhoGAP/DLC1, a tumor-suppressing PLCdelta1 binding protein
  JOURNAL   Adv Enzyme Regul 48, 41-54 (2008)
   PUBMED   18157946
  REMARK    GeneRIF: in PC12 cells PLCdelta1 is recruited to lipid rafts and
            binds to preexisting p122 in Ca2+ dependent manner. This process
            can be triggered by muscarinic stimuli.
REFERENCE   5  (bases 1 to 8079)
  AUTHORS   Yuan,B.Z., Jefferson,A.M., Millecchia,L., Popescu,N.C. and
            Reynolds,S.H.
  TITLE     Morphological changes and nuclear translocation of DLC1 tumor
            suppressor protein precede apoptosis in human non-small cell lung
            carcinoma cells
  JOURNAL   Exp Cell Res 313 (18), 3868-3880 (2007)
   PUBMED   17888903
REFERENCE   6  (bases 1 to 8079)
  AUTHORS   Kawai,K., Yamaga,M., Iwamae,Y., Kiyota,M., Kamata,H., Hirata,H.,
            Homma,Y. and Yagisawa,H.
  TITLE     A PLCdelta1-binding protein, p122RhoGAP, is localized in focal
            adhesions
  JOURNAL   Biochem Soc Trans 32 (Pt 6), 1107-1109 (2004)
   PUBMED   15506980
  REMARK    GeneRIF: Cellular distribution of p122RhoGAP, a GTPase-activating
            protein of Rho small GTPase and an activator of phospholipase
            C-delta(1).
REFERENCE   7  (bases 1 to 8079)
  AUTHORS   Yuan,B.Z., Zhou,X., Durkin,M.E., Zimonjic,D.B., Gumundsdottir,K.,
            Eyfjord,J.E., Thorgeirsson,S.S. and Popescu,N.C.
  TITLE     DLC-1 gene inhibits human breast cancer cell growth and in vivo
            tumorigenicity
  JOURNAL   Oncogene 22 (3), 445-450 (2003)
   PUBMED   12545165
REFERENCE   8  (bases 1 to 8079)
  AUTHORS   Wilson,P.J., McGlinn,E., Marsh,A., Evans,T., Arnold,J., Wright,K.,
            Biden,K., Young,J., Wainwright,B., Wicking,C. and
            Chenevix-Trench,G.
  TITLE     Sequence variants of DLC1 in colorectal and ovarian tumours
  JOURNAL   Hum Mutat 15 (2), 156-165 (2000)
   PUBMED   10649492
REFERENCE   9  (bases 1 to 8079)
  AUTHORS   Sekimata,M., Kabuyama,Y., Emori,Y. and Homma,Y.
  TITLE     Morphological changes and detachment of adherent cells induced by
            p122, a GTPase-activating protein for Rho
  JOURNAL   J Biol Chem 274 (25), 17757-17762 (1999)
   PUBMED   10364218
REFERENCE   10 (bases 1 to 8079)
  AUTHORS   Homma,Y. and Emori,Y.
  TITLE     A dual functional signal mediator showing RhoGAP and phospholipase
            C-delta stimulating activities
  JOURNAL   EMBO J 14 (2), 286-291 (1995)
   PUBMED   7835339
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            JAXUCZ010000016.1.
            
            On Jun 20, 2019 this sequence version replaced XM_017600235.1.
            
            Transcript Variant: This variant (1) represents the longer
            transcript and encodes the longer isoform (1).
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR21887623.2459132.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA5760383, SAMEA5760386
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-806               JAXUCZ010000016.1  61995001-61995806
            807-2005            JAXUCZ010000016.1  62008972-62010170
            2006-2155           JAXUCZ010000016.1  62090941-62091090
            2156-2287           JAXUCZ010000016.1  62096369-62096500
            2288-2321           JAXUCZ010000016.1  62182303-62182336
            2322-2393           JAXUCZ010000016.1  62345336-62345407
            2394-2475           JAXUCZ010000016.1  62349933-62350014
            2476-2539           JAXUCZ010000016.1  62356703-62356766
            2540-3963           JAXUCZ010000016.1  62358246-62359669
            3964-4140           JAXUCZ010000016.1  62360312-62360488
            4141-4300           JAXUCZ010000016.1  62363183-62363342
            4301-4499           JAXUCZ010000016.1  62363463-62363661
            4500-4713           JAXUCZ010000016.1  62365473-62365686
            4714-4828           JAXUCZ010000016.1  62367713-62367827
            4829-5047           JAXUCZ010000016.1  62368437-62368655
            5048-5265           JAXUCZ010000016.1  62369729-62369946
            5266-5439           JAXUCZ010000016.1  62371062-62371235
            5440-8079           JAXUCZ010000016.1  62372180-62374819
FEATURES             Location/Qualifiers
     source          1..8079
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="BN"
                     /db_xref="taxon:10116"
                     /chromosome="16"
                     /map="16q12.1-q12.2"
     gene            1..8079
                     /gene="Dlc1"
                     /gene_synonym="Arhgap7; RhoGAP"
                     /note="DLC1 Rho GTPase activating protein"
                     /db_xref="GeneID:58834"
                     /db_xref="RGD:68416"
     exon            1..806
                     /gene="Dlc1"
                     /gene_synonym="Arhgap7; RhoGAP"
                     /inference="alignment:Splign:2.1.0"
     exon            807..2005
                     /gene="Dlc1"
                     /gene_synonym="Arhgap7; RhoGAP"
                     /inference="alignment:Splign:2.1.0"
     CDS             932..5560
                     /gene="Dlc1"
                     /gene_synonym="Arhgap7; RhoGAP"
                     /note="isoform 1 is encoded by transcript variant 1; rho
                     GTPase-activating protein 7; deleted in liver cancer 1
                     protein homolog; dlc-1; stARD12; p122-RhoGAP; START
                     domain-containing protein 12; rho-type GTPase-activating
                     protein 7; stAR-related lipid transfer protein 12"
                     /codon_start=1
                     /product="rho GTPase-activating protein 7 isoform 1"
                     /protein_id="NP_001357926.1"
                     /db_xref="GeneID:58834"
                     /db_xref="RGD:68416"
                     /translation="MSVALRKRSWEEHVTQQSEQPFNSDEYDIACHHRLASDSLEVRM
                     EKDETLNVDHKEKHASLPDCCRGSETRDSPGRPMGHISPEVDESDSQEGEERFLSLEA
                     STETLVHISDEDTDSDLHLINDSQILPPQRHERDSHESVKGAGSFRKTLSTMQSSQDS
                     HNTWTMAGEAEISAAEGGEERKDGDTISKSLEHCKSTSASETNEVAKVNTFQSLNAKD
                     DITPKTQLLDSLVLVKDVTPETQLLDSAVIAQQHRKPDFPNDDQEKNLHHVVEEDFLV
                     APYTDRGLPLLKADCGSCLLRPPSCPGGMSAENDLERSGFSEYQNKSPLRVSQEGVMQ
                     CLHVRGPVTTQETGDNQVRLRKRKETREDRDRTRLDSMVLLIMKLDQLDQDIENALSA
                     ASSPSSTPTNLRRHVPDLESGSESGTESITVNQTQVNLSSESTDPSSTPASNSGTKPK
                     AMAVPCIAEKETAEIEAKEACDWLRVTGFPQYAQLYEDLLFPIDIALVKREHDFLDRD
                     AIEALCRRLNTLNKCAVMKLEISPHRKRSEDSDEEEPCAISGKWTFQRDSKRWSRLEE
                     FDVFSPKQDPIPGSPDNSRLQSATSRESMLTDLSEHQEVSSIRSLSSTSSSAPTHVPH
                     SGEATTPRTNSVISVCSSSHFVGNEDSFSSLPSPKELSSFSFSMKGHEKNTKSKTRSL
                     LKRMESLKLKGSHHSKHKAPSKLGLIISAPILQEGMDEEKLKQLNCVEISALNGNHIN
                     VPMVRKRSVSNSTQTSSSSSQSETSSAVSTPSPVTRTRSLSTCNKRVGMYLEGFDPFS
                     QSAFNNVTEQNYKNRESYPEDTVFYIPEDHKPGTFPKALSNGSFCPSGNSSVNWRTGS
                     FHGPGHLSLRRENSSDSPKELKRRNSSSSVSSRMSIYDNVPGSILYSSSGELADLENE
                     DIFPELDDILYHVKGMQRIVNQWSEKFSDEGDSDSALDSVSPCPSSPKQIHLDVDHDR
                     RTPSDLDSTGNSLNEPEEPTDIPERRDSGVGASLTRCNRHRLRWHSFQSSHRPSLNSV
                     SLQINCQSVAQMNLLQKYSLLKLTALLEKYTPSNKHGFSWAVPKFMKRIKVPDYKDRS
                     VFGVPLTVNVQRSGQPLPQSIQQAMRYLRNHCLDQVGLFRKSGVKSRIQALRQMNESA
                     EDYVNYEGQSAYDVADMLKQYFRDLPEPLMTNKLSETFLQIYQYVPKDQRLQAIKAAI
                     MLLPDENREVLQTLLYFLSDVTAAVKENQMTPTNLAVCLAPSLFHLNTLKRENSSPRV
                     MQRKQSLGKPDQKDLNENLAATQGLAHMIAECKKLFQVPEEMSRCRNSYTEQELKPLT
                     LEALGHLSNDQPADYRHFLQDCVDGLFKEVKEKFKGWVSYPTSEQAELSYKKVSEGPP
                     LRLWRATIEVPAAPEEILKRLLKEQHLWDVDLLDSKVIEILDSQTEIYQYVQNSMAPH
                     PARDYVVLRTWRTNLPRGACALLFTSVDHDRAPVAGVRVNVLLSRYLIEPCGSGKSKL
                     TYMCRADLRGHMPEWYTKSFGHLCAAEVVKIRDSFSNQSTESKDTRSR"
     exon            2006..2155
                     /gene="Dlc1"
                     /gene_synonym="Arhgap7; RhoGAP"
                     /inference="alignment:Splign:2.1.0"
     exon            2156..2287
                     /gene="Dlc1"
                     /gene_synonym="Arhgap7; RhoGAP"
                     /inference="alignment:Splign:2.1.0"
     exon            2288..2321
                     /gene="Dlc1"
                     /gene_synonym="Arhgap7; RhoGAP"
                     /inference="alignment:Splign:2.1.0"
     exon            2322..2393
                     /gene="Dlc1"
                     /gene_synonym="Arhgap7; RhoGAP"
                     /inference="alignment:Splign:2.1.0"
     exon            2394..2475
                     /gene="Dlc1"
                     /gene_synonym="Arhgap7; RhoGAP"
                     /inference="alignment:Splign:2.1.0"
     exon            2476..2539
                     /gene="Dlc1"
                     /gene_synonym="Arhgap7; RhoGAP"
                     /inference="alignment:Splign:2.1.0"
     exon            2540..3963
                     /gene="Dlc1"
                     /gene_synonym="Arhgap7; RhoGAP"
                     /inference="alignment:Splign:2.1.0"
     exon            3964..4140
                     /gene="Dlc1"
                     /gene_synonym="Arhgap7; RhoGAP"
                     /inference="alignment:Splign:2.1.0"
     exon            4141..4300
                     /gene="Dlc1"
                     /gene_synonym="Arhgap7; RhoGAP"
                     /inference="alignment:Splign:2.1.0"
     exon            4301..4499
                     /gene="Dlc1"
                     /gene_synonym="Arhgap7; RhoGAP"
                     /inference="alignment:Splign:2.1.0"
     exon            4500..4713
                     /gene="Dlc1"
                     /gene_synonym="Arhgap7; RhoGAP"
                     /inference="alignment:Splign:2.1.0"
     exon            4714..4828
                     /gene="Dlc1"
                     /gene_synonym="Arhgap7; RhoGAP"
                     /inference="alignment:Splign:2.1.0"
     exon            4829..5047
                     /gene="Dlc1"
                     /gene_synonym="Arhgap7; RhoGAP"
                     /inference="alignment:Splign:2.1.0"
     exon            5048..5265
                     /gene="Dlc1"
                     /gene_synonym="Arhgap7; RhoGAP"
                     /inference="alignment:Splign:2.1.0"
     exon            5266..5439
                     /gene="Dlc1"
                     /gene_synonym="Arhgap7; RhoGAP"
                     /inference="alignment:Splign:2.1.0"
     exon            5440..8079
                     /gene="Dlc1"
                     /gene_synonym="Arhgap7; RhoGAP"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 gggctgtggc acaaagtacc ctgttctgtt cagatttctg ggggtgtgaa ggagagagaa
       61 gaaggaaatg ctctgcttcc tggattctcg gaattttact tacagacttt tctcaagtcc
      121 aggatgtaaa tttttaagtt attttttaaa gttctaaact ttagaagaat tacggtatta
      181 gaagtctcag ccttcatcta gctgtcttag aaaacttttg aaatgagtct tagcagaagt
      241 gttctccctt taacacagta gaaacgttgt gcgcggaggg gagaggctgc gctttctttc
      301 ttccttgttt tccacgagcc ctgtgggata ggaaggactc taggactttc tgacgctagc
      361 taatcatctc aagagctttg acaaactcac ttcagttctg tgtaaaatca gagcttcaac
      421 gcccaggcgg ctctgctcgg cccctcccct ccctcagctt catgcaggct ttctcacatg
      481 caaacttctc agaggcagga aatactttgc aggcaatctg ggcctgcttg tggaggcccc
      541 tctcgcaaaa catcagtctg catttagaag acagaagtca ctaggatcgc cttgacagga
      601 tcccgagtta gctgaggctc cttccagctg cagagggtgt tttctgttag aatcacacac
      661 tgctgttaaa gagagctgct gagaatagag cccgtgacct cagccagaaa tgtgaactta
      721 gattttggag acactaaccg ggcagactct tctatcctgg ctgcgacagc atcatgcttt
      781 ggcggtaaca gcaactcctg acccagatga caacatggca gattgcactc tcttccttga
      841 cccatgaatt cagaatcttc tctccccgac gtccatccct ttgagtgtcc tatggaatgc
      901 atctccttgt ctgccgttaa actatgatga catgtctgta gctctcagaa agaggagctg
      961 ggaagagcat gtgacccagc agagcgaaca gccttttaat tctgatgagt atgacatagc
     1021 atgtcaccac agactggcat ccgacagcct ggaggtaagg atggagaaag atgaaacctt
     1081 aaatgtggac cataaagaga agcatgcctc tctaccagac tgctgtcgtg ggtcagagac
     1141 aagagactct cctgggaggc caatgggtca catttcaccg gaagtggatg aaagtgacag
     1201 ccaggaagga gaagagcggt ttctatcgct cgaggccagc acagaaacac tagtgcacat
     1261 ttctgatgaa gatactgact ccgatctgca ccttatcaat gactcacaga ttttacctcc
     1321 acaaagacat gaaagggaca gccacgagag tgtcaaagga gcaggctcct tcaggaagac
     1381 actgtccacc atgcaaagta gccaagattc tcacaacacc tggacgatgg ctggtgaagc
     1441 tgagatatct gcagcagaag gaggtgaaga aaggaaagat ggcgacacta taagtaaaag
     1501 cctggagcat tgtaagagta caagtgcaag tgaaacaaat gaggtggcca aagtaaatac
     1561 attccagtct ttgaatgcga aagatgacat cactcctaag acacaactgc ttgattcttt
     1621 ggtactcgtg aaagatgtca cacctgagac acaactgctt gattctgcag taatcgctca
     1681 acaacacagg aaacctgact tccctaacga tgaccaggaa aagaatctgc accatgtagt
     1741 agaagaagac ttcttagtgg ctccttatac agacagaggg ctgccattat taaaagcaga
     1801 ttgcggaagc tgccttctgc ggcctccttc ctgccctggt ggaatgtcag cagaaaatga
     1861 cctggagagg agtgggttct cagaatatca aaacaaaagc cctctcaggg tcagtcagga
     1921 aggtgtcatg cagtgtttac acgtaagggg cccagtgacc acccaggaga ccggagacaa
     1981 tcaagtcaga ctgcgcaaga gaaaggaaac aagggaagat cgagacagga cacggctgga
     2041 ctccatggtg ctgctaatta tgaaactgga ccaactagac caagacatcg agaacgctct
     2101 cagcgctgcc tcctctccat ccagcacccc aacgaacctg cggcggcatg tccctgatct
     2161 ggaatcagga tctgaaagcg gaacagagag tatcacggta aatcagacac aggtgaatct
     2221 gtcttcagag tccaccgatc catcatccac tccagcgagc aattctggaa ccaaacccaa
     2281 agccatggct gtcccatgta ttgcagaaaa ggaaacagct gaaattgaag ccaaggaagc
     2341 ttgcgattgg ctgagagtga ctggtttccc ccagtacgca cagctgtatg aagatctcct
     2401 gtttcctatt gatattgcgc tggtcaagag agaacacgac ttcttggaca gagatgccat
     2461 cgaggctctc tgcaggcggc taaacacgct aaacaagtgt gcagtcatga agctggagat
     2521 cagtcctcac cggaagcgaa gtgaggattc agatgaagag gagccttgtg ccataagcgg
     2581 caagtggact ttccagaggg acagtaagcg gtggtcccgg cttgaagagt tcgatgtctt
     2641 ttctccgaag caggatccga tccctgggtc cccagacaat tcccgtttgc agagcgccac
     2701 aagccgcgaa agcatgctga cagacctcag tgagcaccag gaggtgtcct ctatccgaag
     2761 cctcagcagc accagcagca gcgcccccac ccacgtgccc cacagcgggg aggctacgac
     2821 accccggacc aattctgtca tcagcgtctg ctcctccagc cactttgtag gcaacgagga
     2881 ctctttctcc agcctgccgt cccccaagga actgtccagc ttcagtttta gcatgaaagg
     2941 tcacgagaag aacaccaagt ctaagactcg gagcctgctc aagcggatgg agagcttgaa
     3001 gctcaagggc tcccaccaca gcaagcacaa ggcaccctct aagctggggt tgatcatcag
     3061 cgctcccatc cttcaggagg gcatggacga ggagaagctg aagcagctga actgtgtgga
     3121 gatctcggcc ctcaatggca accacatcaa cgtgcccatg gtacgaaaaa ggagtgtgtc
     3181 taactccacg cagaccagca gtagcagcag tcagtcagag accagcagtg ccgtcagcac
     3241 acccagcccg gtcaccagga cacggagcct cagcacctgt aacaagcggg tgggcatgta
     3301 ccttgagggc ttcgaccctt tcagtcagtc tgcctttaat aatgtgacag agcagaacta
     3361 taagaaccga gagagctacc cggaggacac agtgttctac attcccgaag accacaagcc
     3421 tggcaccttc cccaaggccc tctccaatgg cagtttctgt ccctcgggga acagttctgt
     3481 gaactggagg actggaagct ttcacggccc tggccacctc agcctacgga gagaaaacag
     3541 cagcgacagt cctaaggagc tgaagagacg caactcctcc agctccgtga gcagccgtat
     3601 gagcatctat gacaacgtgc cgggctctat cctttactcc agctcgggcg aactggccga
     3661 cctggagaac gaggacatct tccctgagct cgatgacatt ctctaccacg tgaaggggat
     3721 gcagcggata gtcaaccagt ggtctgaaaa gttctcggac gagggcgact cggactcagc
     3781 cctggactct gtgtctcctt gcccgtcgtc cccaaaacag atacacctgg acgtggacca
     3841 cgaccgaagg acacccagtg acctggacag cacaggcaac tccctcaacg agcccgagga
     3901 gcccaccgat atccctgaaa gaagagactc aggggtgggg gcttccctga ccaggtgtaa
     3961 taggcacaga ctgagatggc atagctttca gagctctcac cggccgagcc tgaactctgt
     4021 gtctctgcag attaactgtc agtctgtggc ccagatgaac ctactgcaga aatactcact
     4081 cctgaaattg acggccctgc tggaaaagta cacgccttcc aacaagcatg gcttcagctg
     4141 ggccgtgccc aagtttatga aaaggatcaa ggttccagac tacaaggacc ggagtgtgtt
     4201 tggggtcccc ctgaccgtga atgtgcagcg ctcaggacag cccctgcctc agagcatcca
     4261 gcaggccatg cgctacctcc gtaaccattg tctggaccag gttgggctct tcaggaagtc
     4321 aggtgtcaaa tcccggatcc aggccctacg ccagatgaac gaaagcgcgg aagattacgt
     4381 caactatgaa ggccaatctg cttacgatgt ggcagacatg ttaaagcaat atttccgaga
     4441 tctccccgag cccctcatga cgaacaaact ctccgaaacg ttcctgcaga tctaccagta
     4501 tgtgcccaag gaccagcgcc tccaagcaat caaggcagcc attatgctcc tgcctgacga
     4561 gaaccgggag gttctccaga cacttctcta tttcttgagc gatgtcacgg cggctgtgaa
     4621 agaaaaccag atgactccca ccaacctggc tgtgtgctta gctccgtctc tcttccacct
     4681 caacaccctg aagcgagaga attcttctcc aagggtaatg caaagaaaac agagtttggg
     4741 caaaccagac cagaaagatc tgaatgaaaa cctagccgcc actcaagggt tggcccacat
     4801 gattgccgag tgcaagaagc tctttcaggt tcctgaggaa atgagccggt gccgtaactc
     4861 atacactgaa caggagctga agcccctgac actggaggcc ttgggacacc tgagtaatga
     4921 tcagcccgct gactacagac acttcctcca ggactgtgtg gatggcctgt tcaaggaggt
     4981 caaagagaag ttcaaaggct gggtcagcta ccctacctct gaacaggccg agctgtccta
     5041 taagaaggta agcgaaggac ccccactaag gctttggagg gcaaccattg aagtccctgc
     5101 ggcccccgag gagatcttaa agcgccttct gaaggagcag cacctctggg atgtcgacct
     5161 gctggactca aaggtgatcg aaatcctgga cagccagact gaaatctacc agtacgtcca
     5221 aaacagcatg gcgccccacc ctgcgaggga ctacgtcgta ctgaggacct ggaggactaa
     5281 cttgcccagg ggagcctgtg ctctcttatt cacctctgtg gatcacgacc gggcacccgt
     5341 ggcgggggtg agggttaatg tgctcctgtc cagatatttg attgaaccct gcgggtcagg
     5401 aaaatccaag ctcacctaca tgtgcagagc tgatttaagg ggccacatgc cggagtggta
     5461 cacaaaatct ttcggacatt tgtgtgcagc tgaagtggta aagatccgag actccttcag
     5521 taatcagagc actgaatcca aggataccag atctaggtga tgaccgaagg gaggcagccg
     5581 tgtctgcaac ctgggtgata ttgctagaac cttgcaagtc cttggaggaa gagttcatct
     5641 gggtctgatt gactacagag attcgacacg ttcttaaagc tgcttcctgt ttgtcttagg
     5701 tctctgttgt agaccttgac tggaatatgt aagacacggc ggggcggggt acctgtatta
     5761 ttcagattgc ttctgagaag caagaactct ttcagcacct aatggaagat acggaagagg
     5821 cgtcgtcgat cttttgcgac atcagcgtct gtgtacctta tttcttattg ttttcggcgt
     5881 actgagggac tgatgtatcc actggaacag ctggtactcg tggacaggtt ttctcactga
     5941 gacgagcaga gaatggtttg cacagaggag tgtgaatgtg tgtgttgctg gctgagtggc
     6001 atagatgaag ggatgggagg cagtgtctgg cacactgaga tgctccacaa aggatgctat
     6061 tatacctggt ttagctcaac ctggaatagc tgactaccca gggatccacc cagaagggga
     6121 atccagatcc cttcctcatc ccccaccccc accccacccc tggttattaa gctttttttt
     6181 ttttttcttt tctgtaaagc tctgctgtcc cataaactgt agcttcccca cgactaggaa
     6241 tttaaccata gactagttca ggaataagca tcagcttcac ctctttgact ggctccaaag
     6301 aaaggctttt ctttacgtga gatagaaaag ctgaacacag acacagccat aagcaaagta
     6361 agaacgcttt ctcatttcca atactgggtg ttgaacccag agccttttat ataccaagca
     6421 agcgctttac agtgaagtca tccccacctc tgttttcact ccttgttctg aggcagtgtc
     6481 tcgccgtgtt gcccaggctg gtcttgaaca actgactcgc tgtatagctc aagttatcta
     6541 ggtctaatag ttgggataag ggactcagca aaacttttta cctgttataa ttcggagtat
     6601 agtgttgctt gtgacataaa ttcaaagata cccaggggaa cccagaacta tgtccccagt
     6661 acgtttaacc caaccaagca caagaggctt ctgattgtgg gtggtggtgt cagtgttcct
     6721 gacagctata tgtgtatttt atcatgcaca gataggtttt ctgagtatta tttttcctgt
     6781 attttttttc tttctctctt tccttatgcc cttgaactgt attttagtaa taatggggga
     6841 tggtaatctc agtgaaaagc gagcagtgca ggttttcgga agcgtggtct ttatacaggt
     6901 tttactgtaa tggtgaggac tgactcaaga cagtattagt aaatttattt tgtatggatc
     6961 gaaacagtga gtaccgtatg aatgagagct gcagaaggat ttttattttg ttataactca
     7021 gttaccttgt cagtattatt tcaaaggttc tttgaagaat ggggggaggg gtcagagtag
     7081 agttttaaaa gtctgagtat ttcaaattat cagtgttgct ttgtgaagat gtgtgtgtgt
     7141 gtgtgtgtgt gtgtgtgtat gtgtgtattc aaccttgatg gcttccagat ctgtaagatt
     7201 gtatgttgta tataccattc tattgagaaa catgtacacg tctgtctact gtgctttcag
     7261 acatagataa agcatgataa agattattat ttaagatata cttgtattta tacctcagat
     7321 attcttttgg gttttgtacc tcaaggttcc cctccccaca ttgtaaatac actttacgtg
     7381 aatacagttt tagtgagaga gaaaaaaaaa gactaaataa aggaagaggt ttataatttg
     7441 ccccatatct atctgtacag agtatattcg gtgagtgcac tattaaatgt ttaaggggaa
     7501 gagtctggcc tgctctctgc cttgcacctt ctccgccctt agaagcaggg atttgcaaag
     7561 cacagggccc cagtgccaac ccaagtccga agctacatta gcgaagagag aagtcagacg
     7621 acgacggaat cattccaagg ccaagctgca actgagccat tcgcccacaa acaggaaagc
     7681 ccggtgaagg ttcaggaagc ctggagagtc tctccagacc aaggtcagaa acgatgctga
     7741 gaaattacga gaatcgagca acggcgaacg gaacgacgct tgtgagcgac gttaaggtca
     7801 ctgaatgatc cacaaaaggt tgctctcccg tgattaactt ccctgctgaa gggaattact
     7861 tacgtctggc cagagaagaa ttcttcagag tatgggactt tccaagaact ggggttaaca
     7921 tcttgtaaca tggccacggt ttgtccacat gactcgtggt ctggatggtg agttcttcaa
     7981 gggcaggctc gtctcctgca catctttatt attcctctac agcagctttc agtgttttgt
     8041 acttgtagac accaaataaa tttatcgttt gctaaagaa
//
