LOCUS       NM_031025               2628 bp    mRNA    linear   ROD 03-APR-2024
DEFINITION  Rattus norvegicus dihydrolipoamide S-acetyltransferase (Dlat),
            mRNA; nuclear gene for mitochondrial product.
ACCESSION   NM_031025 XM_343389
VERSION     NM_031025.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 2628)
  AUTHORS   Mathias,R.A., Greco,T.M., Oberstein,A., Budayeva,H.G.,
            Chakrabarti,R., Rowland,E.A., Kang,Y., Shenk,T. and Cristea,I.M.
  TITLE     Sirtuin 4 is a lipoamidase regulating pyruvate dehydrogenase
            complex activity
  JOURNAL   Cell 159 (7), 1615-1625 (2014)
   PUBMED   25525879
REFERENCE   2  (bases 1 to 2628)
  AUTHORS   Zhao,X., Leon,I.R., Bak,S., Mogensen,M., Wrzesinski,K., Hojlund,K.
            and Jensen,O.N.
  TITLE     Phosphoproteome analysis of functional mitochondria isolated from
            resting human muscle reveals extensive phosphorylation of inner
            membrane protein complexes and enzymes
  JOURNAL   Mol Cell Proteomics 10 (1), M110.000299 (2011)
   PUBMED   20833797
REFERENCE   3  (bases 1 to 2628)
  AUTHORS   Yu,X., Hiromasa,Y., Tsen,H., Stoops,J.K., Roche,T.E. and Zhou,Z.H.
  TITLE     Structures of the human pyruvate dehydrogenase complex cores: a
            highly conserved catalytic center with flexible N-terminal domains
  JOURNAL   Structure 16 (1), 104-114 (2008)
   PUBMED   18184588
REFERENCE   4  (bases 1 to 2628)
  AUTHORS   Lengyel,J.S., Stott,K.M., Wu,X., Brooks,B.R., Balbo,A., Schuck,P.,
            Perham,R.N., Subramaniam,S. and Milne,J.L.
  TITLE     Extended polypeptide linkers establish the spatial architecture of
            a pyruvate dehydrogenase multienzyme complex
  JOURNAL   Structure 16 (1), 93-103 (2008)
   PUBMED   18184587
REFERENCE   5  (bases 1 to 2628)
  AUTHORS   Werner,H.B., Kuhlmann,K., Shen,S., Uecker,M., Schardt,A.,
            Dimova,K., Orfaniotou,F., Dhaunchak,A., Brinkmann,B.G., Mobius,W.,
            Guarente,L., Casaccia-Bonnefil,P., Jahn,O. and Nave,K.A.
  TITLE     Proteolipid protein is required for transport of sirtuin 2 into CNS
            myelin
  JOURNAL   J Neurosci 27 (29), 7717-7730 (2007)
   PUBMED   17634366
REFERENCE   6  (bases 1 to 2628)
  AUTHORS   Yang,D., Song,J., Wagenknecht,T. and Roche,T.E.
  TITLE     Assembly and full functionality of recombinantly expressed
            dihydrolipoyl acetyltransferase component of the human pyruvate
            dehydrogenase complex
  JOURNAL   J Biol Chem 272 (10), 6361-6369 (1997)
   PUBMED   9045657
REFERENCE   7  (bases 1 to 2628)
  AUTHORS   Maury,J., Kerbey,A.L., Priestman,D.A., Patel,M.S., Girard,J. and
            Ferre,P.
  TITLE     Pretranslational regulation of pyruvate dehydrogenase complex
            subunits in white adipose tissue during the suckling-weaning
            transition in the rat
  JOURNAL   Biochem J 311 (Pt 2) (Pt 2), 531-535 (1995)
   PUBMED   7487891
REFERENCE   8  (bases 1 to 2628)
  AUTHORS   Christen,U., Jeno,P. and Gut,J.
  TITLE     Halothane metabolism: the dihydrolipoamide acetyltransferase
            subunit of the pyruvate dehydrogenase complex molecularly mimics
            trifluoroacetyl-protein adducts
  JOURNAL   Biochemistry 32 (6), 1492-1499 (1993)
   PUBMED   8431428
REFERENCE   9  (bases 1 to 2628)
  AUTHORS   Matuda,S., Nakano,K., Ohta,S., Shimura,M., Yamanaka,T.,
            Nakagawa,S., Titani,K. and Miyata,T.
  TITLE     Molecular cloning of dihydrolipoamide acetyltransferase of the rat
            pyruvate dehydrogenase complex: sequence comparison and
            evolutionary relationship to other dihydrolipoamide
            acyltransferases
  JOURNAL   Biochim Biophys Acta 1131 (1), 114-118 (1992)
   PUBMED   1581353
REFERENCE   10 (bases 1 to 2628)
  AUTHORS   Gershwin,M.E., Mackay,I.R., Sturgess,A. and Coppel,R.L.
  TITLE     Identification and specificity of a cDNA encoding the 70 kd
            mitochondrial antigen recognized in primary biliary cirrhosis
  JOURNAL   J Immunol 138 (10), 3525-3531 (1987)
   PUBMED   3571977
COMMENT     PROVISIONAL REFSEQ: This record has not yet been subject to final
            NCBI review. The reference sequence was derived from BC107440.1.
            
            On Nov 1, 2005 this sequence version replaced XM_343389.2.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC107440.1, SRR8487226.85710.1
                                           [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMD00132261,
                                           SAMD00132262 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            gene product(s) localized to mito. :: inferred from homology
            RefSeq Select criteria             :: based on conservation,
                                                  expression, longest protein
            ##RefSeq-Attributes-END##
FEATURES             Location/Qualifiers
     source          1..2628
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /db_xref="taxon:10116"
                     /chromosome="8"
                     /map="8q23"
     gene            1..2628
                     /gene="Dlat"
                     /note="dihydrolipoamide S-acetyltransferase"
                     /db_xref="GeneID:81654"
                     /db_xref="RGD:619859"
     exon            1..262
                     /gene="Dlat"
                     /inference="alignment:Splign:2.1.0"
     CDS             11..1909
                     /gene="Dlat"
                     /EC_number="2.3.1.12"
                     /note="E2; PBC; PDC-E2; 70 kDa mitochondrial autoantigen
                     of primary biliary cirrhosis; dihydrolipoamide
                     acetyltransferase component of pyruvate dehydrogenase
                     complex; PDCE2; pyruvate dehydrogenase complex component
                     E2; dihydrolipoamide S-acetyltransferase (E2 component of
                     pyruvate dehydrogenase complex)"
                     /codon_start=1
                     /product="dihydrolipoyllysine-residue acetyltransferase
                     component of pyruvate dehydrogenase complex,
                     mitochondrial"
                     /protein_id="NP_112287.1"
                     /db_xref="GeneID:81654"
                     /db_xref="RGD:619859"
                     /translation="MWRVCARRVQSAVPRAGFRARWATLKGPRTGPAAVRCGSGIPSY
                     GVRSLCGWSYGSATVPRNRILQQLLGSPSRRSYSLPPHQKVPLPSLSPTMQAGTIARW
                     EKKEGEKISEGDLIAEVETDKATVGFESLEECYMAKILVPEGTRDVPVGSIICITVEK
                     PQDIEAFKNYTLDSATAATQAAPAPAAAPAAAPAAPSASAPGSSYPVHMQIVLPALSP
                     TMTMGTVQRWEKKVGEKLSEGDLLAEIETDKATIGFEVQEEGYLAKILVPEGTRDVPL
                     GTPLCIIVEKQEDIAAFADYRPTEVTSLKPQAPPPVPPPVAAVPPIPQPLAPTPSAAP
                     AGPKGRVFVSPLAKKLAAEKGIDLTQVKGTGPEGRIIKKDIDSFVPTKAAPAAAAAAP
                     PGPRVAPTPAGVFIDIPISNIRRVIAQRLMQSKQTIPHYYLSVDVNMGEVLLVRKELN
                     KMLEGKGKISVNDFIIKASALACLKVPEANSSWMDTVIRQNHVVDVSVAVSTPAGLIT
                     PIVFNAHIKGLETIASDVVSLASKAREGKLQPHEFQGGTFTISNLGMFGIKNFSAIIN
                     PPQACILAIGASEDKLIPADNEKGFDVASVMSVTLSCDHRVVDGAVGAQWLAEFKKYL
                     EKPVTMLL"
     exon            263..364
                     /gene="Dlat"
                     /inference="alignment:Splign:2.1.0"
     exon            365..489
                     /gene="Dlat"
                     /inference="alignment:Splign:2.1.0"
     exon            490..640
                     /gene="Dlat"
                     /inference="alignment:Splign:2.1.0"
     exon            641..767
                     /gene="Dlat"
                     /inference="alignment:Splign:2.1.0"
     exon            768..955
                     /gene="Dlat"
                     /inference="alignment:Splign:2.1.0"
     exon            956..1097
                     /gene="Dlat"
                     /inference="alignment:Splign:2.1.0"
     exon            1098..1165
                     /gene="Dlat"
                     /inference="alignment:Splign:2.1.0"
     exon            1166..1255
                     /gene="Dlat"
                     /inference="alignment:Splign:2.1.0"
     exon            1256..1363
                     /gene="Dlat"
                     /inference="alignment:Splign:2.1.0"
     exon            1364..1479
                     /gene="Dlat"
                     /inference="alignment:Splign:2.1.0"
     exon            1480..1642
                     /gene="Dlat"
                     /inference="alignment:Splign:2.1.0"
     exon            1643..1779
                     /gene="Dlat"
                     /inference="alignment:Splign:2.1.0"
     exon            1780..2602
                     /gene="Dlat"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 cggtggcagt atgtggcgcg tctgtgcgcg gagggtgcag agtgctgttc ccagggccgg
       61 gttcagggct cggtgggcaa ctttgaaggg cccgcggacc ggccccgccg cagttcgttg
      121 cggcagtggg atacccagct atggggtccg ctcgttgtgc ggctggagct acggctctgc
      181 caccgtccca cgtaaccgca tactgcaaca actcctgggg tcgcccagcc gccgctctta
      241 tagcctccct ccgcaccaga aggttccact accttcactt tcccctacaa tgcaggcagg
      301 caccatagcc cgctgggaaa aaaaagaagg ggaaaaaatc agtgagggtg atctaattgc
      361 agaggttgaa actgataaag ccactgttgg atttgagagc ttggaggagt gctatatggc
      421 aaagatcctt gttcctgagg ggaccagaga tgttccagta ggatctatca tttgtatcac
      481 agttgaaaag cctcaggata ttgaggcctt taaaaattat acattggatt cagcaacagc
      541 tgccacacag gcagccccag ccccagctgc agctccagct gctgccccag ctgcaccttc
      601 tgcaagtgct ccaggtagct cctatcccgt tcacatgcag attgttcttc ctgccctctc
      661 cccaaccatg accatgggca ccgtccagag gtgggaaaag aaagtgggag agaagctgag
      721 tgaaggagac ttgctggcag agatagagac cgacaaggcc accataggct ttgaagtaca
      781 agaagaaggt tatctggcaa aaatcctggt ccctgaaggc acaagggatg ttcctctggg
      841 aaccccgcta tgtatcatag tagaaaaaca ggaagatata gcagcatttg cagactacag
      901 gccaacagaa gtgaccagct taaagccaca ggcaccacca cctgtcccac ccccagtggc
      961 agctgttcct cccatccccc agcctttagc acctaccccc tcagccgctc ctgctggacc
     1021 aaagggaagg gtgttcgtta gccctcttgc aaagaaattg gcagcagaga aagggattga
     1081 cctcacccaa gttaaaggga cgggaccaga aggcagaatc atcaagaagg acattgactc
     1141 ttttgtgcct actaaggctg ctcctgccgc tgcagcagct gctcccccgg gtccaagagt
     1201 ggcaccaact cctgcaggtg tcttcataga catccccatc agcaacattc gtcgagtgat
     1261 tgcgcagagg ctcatgcagt cgaagcagac tatacctcat tattaccttt ctgttgatgt
     1321 aaatatggga gaggtgctgt tggtgcggaa ggaacttaat aagatgcttg aaggtaaagg
     1381 aaaaatctcc gtcaatgact tcatcataaa agcttcagct ttggcctgcc tgaaagttcc
     1441 tgaagcaaac tcatcttgga tggacacagt tatacgacaa aatcatgtgg ttgatgtcag
     1501 cgttgctgtc agtacccctg caggacttat cacccctatt gtgtttaatg cacacataaa
     1561 aggactggaa accattgcta gtgatgttgt ttctttagcc tccaaagcaa gagagggtaa
     1621 acttcagcct cacgagttcc agggtgggac atttacaatc tccaacttag ggatgttcgg
     1681 aattaagaat ttctctgcga ttattaaccc acctcaggca tgtattttgg caattggtgc
     1741 ttccgaggat aaactgatcc ctgcagataa tgagaaaggc tttgacgtgg ctagtgtgat
     1801 gtctgtcaca ctcagctgtg atcatcgagt tgtggatgga gcagttggag cccagtggct
     1861 tgctgaattc aaaaagtacc ttgaaaagcc tgtaactatg ttattataat ttccctgcga
     1921 acttctattc ttcctctgct gacattatat ttatatgtta ttaaacaggt ggttctttta
     1981 aagtttcact agttaatttt attattgagt cagtccagat aaattatttg taatagacat
     2041 aaatgaatct tttgtaaatg ccagttacac ccaaatgttt taccccttta ataattacac
     2101 atcggtttaa agctgtgtac tcctagtgaa gggacatgtg gcctaactct ttggtgagaa
     2161 gaccttcctg ggagtataga atgggcagga ttgtggcttc ctgcagacag actcataaaa
     2221 gtaacctgga tgagatcttg gagtttagat ttaattgatt caacttattt aagtgtgagg
     2281 gaaacagtcg tgaaaattac ttcttatggg aggagtttga atttacgttg gaatctaaca
     2341 gttaaagtct cctgtcacat gaccttagtt tatcactcag taagtgaaac ttttaagagt
     2401 cagcattttc ttagacctct tcaaaccaac tattattttt atatgaatct ctaaataaaa
     2461 tagctttccc cacaagataa agatataaac cagcttcttg gtataaatga aattttatgt
     2521 atttatttat aaacaatttc aatgtataac ttgagaattc tgcagtttcc tccaaataaa
     2581 agaacatgca tattgaaatg aaaaaaaaaa aaaaaaaaaa aaaaaaaa
//
