LOCUS       NM_031720               6300 bp    mRNA    linear   ROD 30-MAR-2024
DEFINITION  Rattus norvegicus iodothyronine deiodinase 2 (Dio2), mRNA.
ACCESSION   NM_031720
VERSION     NM_031720.5
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 6300)
  AUTHORS   Bai,Z., Chai,X.R., Yoon,M.J., Kim,H.J., Lo,K.A., Zhang,Z.C., Xu,D.,
            Siang,D.T.C., Walet,A.C.E., Xu,S.H., Chia,S.Y., Chen,P., Yang,H.,
            Ghosh,S. and Sun,L.
  TITLE     Dynamic transcriptome changes during adipose tissue energy
            expenditure reveal critical roles for long noncoding RNA regulators
  JOURNAL   PLoS Biol 15 (8), e2002176 (2017)
   PUBMED   28763438
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6300)
  AUTHORS   Martinez-deMena,R., Calvo,R.M., Garcia,L. and Obregon,M.J.
  TITLE     Effect of glucocorticoids on the activity, expression and proximal
            promoter of type II deiodinase in rat brown adipocytes
  JOURNAL   Mol Cell Endocrinol 428, 58-67 (2016)
   PUBMED   26994513
  REMARK    GeneRIF: Glucocorticoids inhibited the adrenergic stimulation of
            Dio2 at the transcriptional level in brown adipocytes.
REFERENCE   3  (bases 1 to 6300)
  AUTHORS   da Conceicao,R.R., Laureano-Melo,R., Oliveira,K.C., de Carvalho
            Melo,M.C., Kasamatsu,T.S., de Barros Maciel,R.M., de Souza,J.S. and
            Giannocco,G.
  TITLE     Antidepressant behavior in thyroidectomized Wistar rats is induced
            by hippocampal hypothyroidism
  JOURNAL   Physiol Behav 157, 158-164 (2016)
   PUBMED   26861177
  REMARK    GeneRIF: This study demonstrated that reduction of the Dio2 mRNA
            expression in hippocampus of thyroidectomized Wistar rats.
REFERENCE   4  (bases 1 to 6300)
  AUTHORS   Werneck de Castro,J.P., Fonseca,T.L., Ueta,C.B., McAninch,E.A.,
            Abdalla,S., Wittmann,G., Lechan,R.M., Gereben,B. and Bianco,A.C.
  TITLE     Differences in hypothalamic type 2 deiodinase ubiquitination
            explain localized sensitivity to thyroxine
  JOURNAL   J Clin Invest 125 (2), 769-781 (2015)
   PUBMED   25555216
  REMARK    GeneRIF: These studies reveal that tissue-specific differences in
            D2 ubiquitination are an inherent property of the TRH/TSH feedback
            mechanism
REFERENCE   5  (bases 1 to 6300)
  AUTHORS   Mariotti,M., Ridge,P.G., Zhang,Y., Lobanov,A.V., Pringle,T.H.,
            Guigo,R., Hatfield,D.L. and Gladyshev,V.N.
  TITLE     Composition and evolution of the vertebrate and mammalian
            selenoproteomes
  JOURNAL   PLoS One 7 (3), e33066 (2012)
   PUBMED   22479358
REFERENCE   6  (bases 1 to 6300)
  AUTHORS   Salvatore,D., Harney,J.W. and Larsen,P.R.
  TITLE     Mutation of the Secys residue 266 in human type 2 selenodeiodinase
            alters 75Se incorporation without affecting its biochemical
            properties
  JOURNAL   Biochimie 81 (5), 535-538 (1999)
   PUBMED   10403186
REFERENCE   7  (bases 1 to 6300)
  AUTHORS   Remmers,E.F., Griffiths,M.M., Longman,R.E., Gulko,P.S.,
            Kawahito,Y., Chen,S., Chang,L., Shepard,J., Ge,L., Dracheva,S.,
            Wang,J.P., Joe,B., Cannon,G.W. and Wilder,R.L.
  TITLE     An integrated rat genetic map: analysis of linkage conservation
            with the mouse and human maps
  JOURNAL   Transplant Proc 31 (3), 1549-1554 (1999)
   PUBMED   10330996
REFERENCE   8  (bases 1 to 6300)
  AUTHORS   Gondou,A., Toyoda,N., Nishikawa,M., Tabata,S., Yonemoto,T.,
            Ogawa,Y., Tokoro,T., Sakaguchi,N., Wang,F. and Inada,M.
  TITLE     Induction of type 2 deiodinase activity by cyclic guanosine
            3',5'-monophosphate in cultured rat glial cells
  JOURNAL   Thyroid 8 (7), 615-622 (1998)
   PUBMED   9709916
REFERENCE   9  (bases 1 to 6300)
  AUTHORS   Croteau,W., Davey,J.C., Galton,V.A. and St Germain,D.L.
  TITLE     Cloning of the mammalian type II iodothyronine deiodinase. A
            selenoprotein differentially expressed and regulated in human and
            rat brain and other tissues
  JOURNAL   J Clin Invest 98 (2), 405-417 (1996)
   PUBMED   8755651
  REMARK    GeneRIF: The proteins encoded by rat and human Dio2 (deiodinase,
            iodothyronine, type II) genes are selenoproteins.
REFERENCE   10 (bases 1 to 6300)
  AUTHORS   Nowak,F.V.
  TITLE     Cloning of two hypothalamic cDNAs encoding tissue-specific
            transcripts in the preoptic area and testis
  JOURNAL   Mol Endocrinol 4 (8), 1205-1210 (1990)
   PUBMED   2293025
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from JAXUCZ010000006.1.
            
            On Oct 9, 2018 this sequence version replaced NM_031720.4.
            
            Summary: The protein encoded by this gene belongs to the
            iodothyronine deiodinase family. It catalyzes the conversion of
            prohormone thyroxine (3,5,3',5'-tetraiodothyronine, T4) to the
            bioactive thyroid hormone (3,5,3'-triiodothyronine, T3) by outer
            ring 5'-deiodination. This gene is expressed in a few tissues in
            rat, including brain, pituitary and brown adipose tissue. It is
            thought to be responsible for the 'local' production of T3, and
            thus important in influencing thyroid hormone action in these
            tissues. Studies in rat suggest that this gene may play an
            important role in the regulation of lipid metabolism and
            thermogenesis, and in osteoarthritis pathogenesis by enhancing the
            chondrocyte hypertrophy and inflammatory response. This protein is
            a selenoprotein containing the non-standard amino acid,
            selenocysteine (Sec), which is encoded by the UGA codon that
            normally signals translation termination. The 3' UTRs of
            selenoprotein mRNAs contain a conserved stem-loop structure,
            designated the Sec insertion sequence (SECIS) element, that is
            necessary for the recognition of UGA as a Sec codon, rather than as
            a stop signal. Unlike the other two members (DIO1 and DIO3) of this
            enzyme family, the mRNA for this gene contains an additional
            in-frame UGA codon that has been reported (in human) to function
            either as a Sec or a stop codon, resulting in two potential
            isoforms with one or two Sec residues; however, only the upstream
            Sec (conserved with the single Sec residue found at the active site
            in DIO1 and DIO3) was shown to be essential for enzyme activity
            (PMID:10403186). In addition, the lack of conservation of the
            protein extension past the second TGA codon suggests that the
            one-Sec containing isoform represents the canonical form. [provided
            by RefSeq, Oct 2018].
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: U53505.1, SRR26360190.907832.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA5760383, SAMEA5760389
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            protein contains selenocysteine :: PMID: 8755651
            RefSeq Select criteria          :: based on single protein-coding
                                               transcript
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-785               JAXUCZ010000006.1  115409810-115410594 c
            786-6300            JAXUCZ010000006.1  115396308-115401822 c
FEATURES             Location/Qualifiers
     source          1..6300
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="BN"
                     /db_xref="taxon:10116"
                     /chromosome="6"
                     /map="6q31"
     gene            1..6300
                     /gene="Dio2"
                     /gene_synonym="5DII; DIOII"
                     /note="iodothyronine deiodinase 2"
                     /db_xref="GeneID:65162"
                     /db_xref="RGD:68418"
     exon            1..785
                     /gene="Dio2"
                     /gene_synonym="5DII; DIOII"
                     /inference="alignment:Splign:2.1.0"
     CDS             564..1352
                     /gene="Dio2"
                     /gene_synonym="5DII; DIOII"
                     /EC_number="1.21.99.4"
                     /note="type 2 DI; type-II 5'-deiodinase; deiodinase,
                     iodothyronine, type 2; deiodinase, iodothyronine, type II"
                     /codon_start=1
                     /transl_except=(pos:951..953,aa:Sec)
                     /product="type II iodothyronine deiodinase"
                     /protein_id="NP_113908.4"
                     /db_xref="GeneID:65162"
                     /db_xref="RGD:68418"
                     /translation="MGLLSVDLLITLQILPVFFSNCLFLALYDSVILLKHVALLLSRS
                     KSTRGEWRRMLTSEGLRCVWNSFLLDAYKQVKLGEDAPNSSVVHVSNPEAGNNCASEK
                     TADGAECHLLDFASAERPLVVNFGSATUPPFTRQLPAFRQLVEEFSSVADFLLVYIDE
                     AHPSDGWAVPGDSSMSFEVKKHRNQEDRCAAAHQLLERFSLPPQCQVVADRMDNNANV
                     AYGVAFERVCIVQRRKIAYLGGKGPFSYNLQEVRSWLEKNFSKR"
     exon            786..6300
                     /gene="Dio2"
                     /gene_synonym="5DII; DIOII"
                     /inference="alignment:Splign:2.1.0"
     regulatory      5987..6061
                     /regulatory_class="recoding_stimulatory_region"
                     /gene="Dio2"
                     /gene_synonym="5DII; DIOII"
                     /note="SECIS_element"
                     /function="essential for recoding UGA to specify
                     selenocysteine"
ORIGIN      
        1 cccaaacaac ccttgcgctt acagcacagt taagaaattg gcagtgtaag aagccagact
       61 gcgtttaaag gaacatgaaa agctaaaaga aatttttacc atctctgcag tctcataggt
      121 gcctggaaat gaaagtagaa cttcctgtct ttaacggact ctgacagggg taaactggat
      181 tagggactgg cacgccaggg tgttgttgtt gttgttgttg ttgttttgtt gttgttgttg
      241 ctgggagtgg tggtgttttg tttttttttt ttaaccatct taaatcctgg aaaaaaaatg
      301 catctcggaa ttgaatgaat tgatgggtcc actccaactg ctgggctgca gagactgaac
      361 ttggtctttc catttctgct tctttcaaag aagagacaag ttgggatccc ttctaattta
      421 gcttttctcc ctctccctca ccccccaccc ccactcccaa ccttcccctt ggtcccccac
      481 ttctctacca ccaccttcct ttgcaaaaag ggggtgaccg gggaagcaga gtgcacagga
      541 gactgactga ggaggtaaag aagatgggac tcctcagcgt agacttgctg atcactcttc
      601 agattctgcc agtctttttc tccaactgcc tcttcctggc gctctatgac tcggtcattc
      661 tgctcaagca cgtggcgctg cttctcagcc gctccaagtc cactcgcgga gagtggaggc
      721 gcatgctgac ctcagaagga ctacgctgtg tctggaacag ctttctccta gacgcctaca
      781 aacaggttaa attgggtgaa gatgctccca attccagtgt ggtgcacgtg tccaatcctg
      841 aagcaggtaa caattgtgcc tcagagaaga cggcggatgg ggctgaatgc caccttcttg
      901 actttgccag tgcagagcgc ccactggtgg tcaactttgg ttcagcgacc tgaccacctt
      961 ttactaggca actgccagcc ttccgccagt tggtggaaga gttctcctcg gtggctgact
     1021 tcctgttggt atacattgat gaggctcacc cttcagatgg ctgggcagtg cctggggact
     1081 cctctatgtc ttttgaggtt aagaagcacc ggaaccaaga ggaccgatgt gctgcagccc
     1141 accagctcct ggagcgtttc tccttgccgc cccagtgtca agttgtggct gaccgcatgg
     1201 acaataatgc caacgtagct tatggggtag cctttgaacg tgtgtgcatc gtgcagagac
     1261 ggaaaattgc ttacttagga gggaagggcc ccttcagcta taacctgcaa gaagtccgaa
     1321 gttggctgga gaagaatttc agcaagagat gaattctaga ttagctggat aaaagtgtga
     1381 ttgtaataga gtttattatt ttaaaagaat gtgtaaaagt tgagaactga actgaatcta
     1441 ttatttcaac ggaatcccat cgcctcactg aaagacgggt catacctgtc agaagatctt
     1501 gaacctcttt aacatctcag tacttccttt agtacacaaa cggcatctgg ttaaacagca
     1561 gccctgttac ctactttcag agctaactgg tccaaaatgg agggccagaa accccgttgc
     1621 agccttgttc attctcgctt gtgaaagaag tgatgctgcc ctatgtgtgc tctgggacta
     1681 gaggacaaaa gttcccagaa aacctattgt taagggagct actacctatg atctgattaa
     1741 gtgtattgtc tcagtcagct ttagtcacca gaagtaaacc caactggaaa gtccccccaa
     1801 atacatactt ataatgtgat ctggaagcca tgtcatgcag ttaggactga agtgttagtt
     1861 ctagccaagc aatgtcctgt gttagtcctc tagccccgag tatgcatgtg aataccaaga
     1921 atggccccaa cctggagggt tgagatgacc acagaaatat ggcagatgga gaaagaattg
     1981 ataagcaaag acagaggaag cagaacgatg aggaaattgc atagtgcttt atctggaagc
     2041 tgtaggtaat gagagctgta cgactgcatc agcattgaag catgcacaca gcgcggggga
     2101 acaaaggtgt taactaaaaa cagcgtaggg ggatgcacat gatgagaagt agatgggtag
     2161 caatactccc agactgaaag gaagtgggca atggaaaaga taaaaacagg cggacacttt
     2221 taatgtggag agaagtacgt tgtgggacta attgtgatat acctatttct gtgggtaaca
     2281 cccattgaga gaaaatccgg ggcttcttgt gctgctgggt ttttcctgct gaattcctag
     2341 ttacaatgct ccacacctct gtagcattgt tttctctggg tgctgtgtga agggtgtaag
     2401 aggcaggtac cttagctgtg cagtctctgc cctcgcctcc tgcagaagaa ttctaatgct
     2461 aagagatttc tttacataaa catgagagca atttactcag ataatctcta cccatagttt
     2521 tgagggctta cacaagctta ctcttggtac cacaggaatt tggtaaaatg gaatttccat
     2581 tgacatttga atttggactt tttttttttt ttacttcaac tgagattaac ccattagttg
     2641 aagtttgata catttctctt aaaatacaag cagcacagct gtatccacaa gcaataccta
     2701 aagatcacaa cctcagaaaa agaaaaacac ccaaaaaaac cccaaaaaac ccaaaaccaa
     2761 aacacaccaa cagatcaatc gatgtattat aattattttt taaactcaaa gacatgaaga
     2821 tctcaacata tggctatgct tacatgccaa atcaaggact ttatgtctat tccactcaat
     2881 gattgaacca caccgttgaa ctagtttgga aaagacaatc aaaaattaaa agtaagttat
     2941 taaaatatct gtcccaaatg agaaaatagg aagtgttgtt tttaaataag aatcttttaa
     3001 ataagaaaat aagagtcttg tttttaagac tgggagcaag gaataagaca gatcgctagc
     3061 ccacgtattg tttagttacg gacgcagcat tgtggctaat atttttggca gcatctaaga
     3121 tctccagtgt gttaaagtct gtaagagaga cacccacaag gaaagcaaac ttctaaattg
     3181 caacggtgct aatgagcttt tgtaaaatca tattgaagat agattactcc aggttcctca
     3241 agtgttaagg ccttcagtcg aggcccagga gtgatccctc attcatcttc atcttcaaaa
     3301 tggtgttgag attgatgcag ctccctagag aagcaaaatg aaaaattggc cgctccacac
     3361 ccacatcgtc atcctcaagt gtccccttcg gtttccacgt gcgacagtga agcggaatgt
     3421 cagagagtgg gtggggggat gagtttttgg ctaacgccat gctagttgtc tggggaaagg
     3481 ggataatggg aaaaagtcgt ccagctgtgt tacattttta aagaaagaga aggtaatgtg
     3541 cgcgtaccat ttgccttctg gaggataggc cagtggacga aggaagggga ggaagcaaat
     3601 gtacaccgat gtaggctgac cgtcagggac acagacatgt tcagcagagc atggcggaaa
     3661 cactggaaaa aaaccgttga agggtccagg aagaaagtga ctagcagagc tcaggaatct
     3721 tacactgtat agaatgggtt ggtctcccag gtaatgataa gcctctagtt atataaccta
     3781 tttatctcaa tgtaaacgtc tatgaattcc tataatgtgc cttttataaa atgaacaaga
     3841 agtcattcat tcagagacga gggaaaaaaa gatcaatgct cagctattgt gctcagagat
     3901 ccctattagg ctgtcggtac ccacccacct aacatgcctc tcataatgta tattcctttg
     3961 aagagttata gttgtaaatc tcccaaggaa acacaaatat gtgtatatat attctcacaa
     4021 ggacacacac acacacacac acacacacac acacacacac acacacacac tcctcttgga
     4081 gttatttttc ttggaacata aagatttccc cccataggat atgtttacat ggggatatca
     4141 ctttatactt tcaaactgaa ttggcccatc catctactat tgaaataatt tctcagaact
     4201 tagcatagca cctggacttc ctaagcacta agtgtttgaa agacatctta gacctattca
     4261 gaactctctt tagtttgtcg ggtagaggaa gtcatccggg caatgactct tttcgagtga
     4321 gcatgaagga agtgcctgtc cagcaaatgg agacgtattt tgaacggatt aaggggacct
     4381 ctgtggaggc aagtcagtca ttgttcttgc tctgtgttgc caaagtgcca gttgtttaac
     4441 acacgtgtta gcttcattca tgctttagat ctctgctcct gtggcatcca tagattgtat
     4501 ccccggattt ttggtaatat cagcttctaa gtggtctttt aacttagttc agtttttcta
     4561 ataatcaata gacacccgtt gatcccttta cctgaatgta tttgactatt tgggacttgg
     4621 aataaaattc ataaaatggg gtgaggtcac aggtggggac tgcctcaccc aaactactgg
     4681 aggggaaggt gggggatagt tgggggataa tattgatgct gttcgtagaa ttctcaggtg
     4741 ggtacaagct gcccttggca tgccctatag cctccaacca cagccagagg gatttgctga
     4801 tgtaccaggc ttcccactgt gtatgtacat ggtaagagga tcaacctgga cattagttcc
     4861 acctgacctc tgactgatgg tttggaaaac aagtttaatt agaatccctt ggccactgca
     4921 gaggggaaaa ttaggctgat ttctgtccca ctggaggata aaggaagact taggccagcg
     4981 cttcagcttg tcaggacacg cccaatgtcg tttttcttag tggaaattga ttgtgagtct
     5041 ttttaattcc aggagcgtcc tttatgacac agcttcaaaa cagaacccaa gtattttgag
     5101 ttatactcca acttctgcca ttgccttaaa tgtagaagcc aaaccttctc tttatggact
     5161 ttcagagtgg acacaaagga tcacttgctg ctagatgaca gtagtgtctt gggactgtcc
     5221 agtcccttta ggaaaggaag gatctgcagc tgcatgaact gggaagctgc taacggggag
     5281 gaattagcgc ccccacccca aacccccagc cccaaggcct gggcttacat atttggaaag
     5341 atttgaaatg aaacttttta tgctcagagg aaggctgccg aatgtcagtg gatgagttta
     5401 gggtgagcaa acatcagctc tattcttcat accctgtgtt ctagataaca ccagaaacaa
     5461 ggtctcagac ttaacagact cagctctgtt ccaaggtgag tctgaaccag caaaaagcaa
     5521 acacatacag atatccaaac aagactgctc acgcaagcca gggcttgctg cctgtctcgg
     5581 gcaacagcac cagggctcta gggagttcgt ttaatattta ctgagacttc agagacccag
     5641 cagatgttta atgaagtcac tattttggct caaactgccc tcccccatct gtcttgagaa
     5701 aaaaaattcc aacaggtaaa catacaaatg aaagagaccc acagaagggg atgggaaata
     5761 agtaaaattc tctcaagact gcttcagacc atgccatgtg gtttcgatgt gtcaggagtc
     5821 ggggatgaga agacctatgt acctagtact ttataaccaa agcaatcaaa aggtcttggg
     5881 gtagggaatg ttggccagtt ttgtttagtt tttggatcat gtttccacca gactcacaag
     5941 cccgtgtaac cagcacccaa aagaggaaac cgagatgttc ggagctcact ggtgtgcgaa
     6001 tgataactac tgacgaaaga gtcatctgct cagtctgtgg ttggatgtag tcacacgagt
     6061 ctgcctctcc atctctgtct ccttagcaag gaggacattt gggtcctggt gcaaagagtg
     6121 ctgagtcagt gcgactgagg gcacgtgtgt ccacctgtgg ttctcagttg cagagaaaga
     6181 agagccaagc tttggggatt atcagcagga agagtgttat aatattggtg ctgagcggta
     6241 tgtgctgttt cacaacttgt tcttatattt taataaactt cgaataaaag aatagagtta
//
