LOCUS       NM_001107393            5617 bp    mRNA    linear   ROD 13-FEB-2025
DEFINITION  Rattus norvegicus diaphanous-related formin 1 (Diaph1), transcript
            variant 1, mRNA.
ACCESSION   NM_001107393 XM_001065947 XM_226014
VERSION     NM_001107393.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 5617)
  AUTHORS   Wang,X., Tang,P., Guo,F., Zhang,M., Yan,Y., Huang,M., Chen,Y.,
            Zhang,L. and Zhang,L.
  TITLE     mDia1 and Cdc42 Regulate Activin B-Induced Migration of Bone
            Marrow-Derived Mesenchymal Stromal Cells
  JOURNAL   Stem Cells 37 (1), 150-162 (2019)
   PUBMED   30358011
  REMARK    GeneRIF: Study demonstrated that the RhoA-mDia1 and Cdc42 pathways
            regulate Activin B-induced BMSC migration.
REFERENCE   2  (bases 1 to 5617)
  AUTHORS   Qu,X., Yuan,F.N., Corona,C., Pasini,S., Pero,M.E., Gundersen,G.G.,
            Shelanski,M.L. and Bartolini,F.
  TITLE     Stabilization of dynamic microtubules by mDia1 drives Tau-dependent
            Abeta1-42 synaptotoxicity
  JOURNAL   J Cell Biol 216 (10), 3161-3178 (2017)
   PUBMED   28877993
  REMARK    GeneRIF: these results uncover a novel role for mDia1 in
            Abeta-mediated synaptotoxicity and demonstrate that inhibition of
            MT dynamics and accumulation of PTMs are driving factors for the
            induction of tau-mediated neuronal damage.
REFERENCE   3  (bases 1 to 5617)
  AUTHORS   Stritt,S., Nurden,P., Turro,E., Greene,D., Jansen,S.B.,
            Westbury,S.K., Petersen,R., Astle,W.J., Marlin,S., Bariana,T.K.,
            Kostadima,M., Lentaigne,C., Maiwald,S., Papadia,S., Kelly,A.M.,
            Stephens,J.C., Penkett,C.J., Ashford,S., Tuna,S., Austin,S.,
            Bakchoul,T., Collins,P., Favier,R., Lambert,M.P., Mathias,M.,
            Millar,C.M., Mapeta,R., Perry,D.J., Schulman,S., Simeoni,I.,
            Thys,C., Gomez,K., Erber,W.N., Stirrups,K., Rendon,A.,
            Bradley,J.R., van Geet,C., Raymond,F.L., Laffan,M.A., Nurden,A.T.,
            Nieswandt,B., Richardson,S., Freson,K., Ouwehand,W.H. and
            Mumford,A.D.
  CONSRTM   BRIDGE-BPD Consortium
  TITLE     A gain-of-function variant in DIAPH1 causes dominant
            macrothrombocytopenia and hearing loss
  JOURNAL   Blood 127 (23), 2903-2914 (2016)
   PUBMED   26912466
REFERENCE   4  (bases 1 to 5617)
  AUTHORS   Ercan-Sencicek,A.G., Jambi,S., Franjic,D., Nishimura,S., Li,M.,
            El-Fishawy,P., Morgan,T.M., Sanders,S.J., Bilguvar,K., Suri,M.,
            Johnson,M.H., Gupta,A.R., Yuksel,Z., Mane,S., Grigorenko,E.,
            Picciotto,M., Alberts,A.S., Gunel,M., Sestan,N. and State,M.W.
  TITLE     Homozygous loss of DIAPH1 is a novel cause of microcephaly in
            humans
  JOURNAL   Eur J Hum Genet 23 (2), 165-172 (2015)
   PUBMED   24781755
REFERENCE   5  (bases 1 to 5617)
  AUTHORS   Baarlink,C., Wang,H. and Grosse,R.
  TITLE     Nuclear actin network assembly by formins regulates the SRF
            coactivator MAL
  JOURNAL   Science 340 (6134), 864-867 (2013)
   PUBMED   23558171
REFERENCE   6  (bases 1 to 5617)
  AUTHORS   Shimada,A., Nyitrai,M., Vetter,I.R., Kuhlmann,D., Bugyi,B.,
            Narumiya,S., Geeves,M.A. and Wittinghofer,A.
  TITLE     The core FH2 domain of diaphanous-related formins is an elongated
            actin binding protein that inhibits polymerization
  JOURNAL   Mol Cell 13 (4), 511-522 (2004)
   PUBMED   14992721
REFERENCE   7  (bases 1 to 5617)
  AUTHORS   Li,F. and Higgs,H.N.
  TITLE     The mouse Formin mDia1 is a potent actin nucleation factor
            regulated by autoinhibition
  JOURNAL   Curr Biol 13 (15), 1335-1340 (2003)
   PUBMED   12906795
REFERENCE   8  (bases 1 to 5617)
  AUTHORS   Watanabe,N., Kato,T., Fujita,A., Ishizaki,T. and Narumiya,S.
  TITLE     Cooperation between mDia1 and ROCK in Rho-induced actin
            reorganization
  JOURNAL   Nat Cell Biol 1 (3), 136-143 (1999)
   PUBMED   10559899
REFERENCE   9  (bases 1 to 5617)
  AUTHORS   Lynch,E.D., Lee,M.K., Morrow,J.E., Welcsh,P.L., Leon,P.E. and
            King,M.C.
  TITLE     Nonsyndromic deafness DFNA1 associated with mutation of a human
            homolog of the Drosophila gene diaphanous
  JOURNAL   Science 278 (5341), 1315-1318 (1997)
   PUBMED   9360932
REFERENCE   10 (bases 1 to 5617)
  AUTHORS   Watanabe,N., Madaule,P., Reid,T., Ishizaki,T., Watanabe,G.,
            Kakizuka,A., Saito,Y., Nakao,K., Jockusch,B.M. and Narumiya,S.
  TITLE     p140mDia, a mammalian homolog of Drosophila diaphanous, is a target
            protein for Rho small GTPase and is a ligand for profilin
  JOURNAL   EMBO J 16 (11), 3044-3056 (1997)
   PUBMED   9214622
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            JAXUCZ010000018.1.
            
            On Jun 3, 2021 this sequence version replaced NM_001107393.2.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            RNAseq introns :: partial sample support SAMN16676787, SAMN16676806
                              [ECO:0000350]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            inferred exon combination :: based on alignments, homology
            RefSeq Select criteria    :: based on conservation, expression,
                                         longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-211               JAXUCZ010000018.1  30020070-30020280   c
            212-238             JAXUCZ010000018.1  29991904-29991930   c
            239-394             JAXUCZ010000018.1  29990560-29990715   c
            395-496             JAXUCZ010000018.1  29989228-29989329   c
            497-627             JAXUCZ010000018.1  29988813-29988943   c
            628-714             JAXUCZ010000018.1  29988550-29988636   c
            715-778             JAXUCZ010000018.1  29987631-29987694   c
            779-918             JAXUCZ010000018.1  29986198-29986337   c
            919-1027            JAXUCZ010000018.1  29983014-29983122   c
            1028-1138           JAXUCZ010000018.1  29981917-29982027   c
            1139-1257           JAXUCZ010000018.1  29981651-29981769   c
            1258-1374           JAXUCZ010000018.1  29980675-29980791   c
            1375-1490           JAXUCZ010000018.1  29979578-29979693   c
            1491-1555           JAXUCZ010000018.1  29978802-29978866   c
            1556-1735           JAXUCZ010000018.1  29976961-29977140   c
            1736-2434           JAXUCZ010000018.1  29975072-29975770   c
            2435-2549           JAXUCZ010000018.1  29974297-29974411   c
            2550-2558           JAXUCZ010000018.1  29973816-29973824   c
            2559-2657           JAXUCZ010000018.1  29937611-29937709   c
            2658-2752           JAXUCZ010000018.1  29934057-29934151   c
            2753-2854           JAXUCZ010000018.1  29933659-29933760   c
            2855-3094           JAXUCZ010000018.1  29933191-29933430   c
            3095-3224           JAXUCZ010000018.1  29932929-29933058   c
            3225-3349           JAXUCZ010000018.1  29931921-29932045   c
            3350-3514           JAXUCZ010000018.1  29930984-29931148   c
            3515-3650           JAXUCZ010000018.1  29930745-29930880   c
            3651-3737           JAXUCZ010000018.1  29928631-29928717   c
            3738-5617           JAXUCZ010000018.1  29920889-29922768   c
FEATURES             Location/Qualifiers
     source          1..5617
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="BN"
                     /db_xref="taxon:10116"
                     /chromosome="18"
                     /map="18p11"
     gene            1..5617
                     /gene="Diaph1"
                     /gene_synonym="Diap1; DRF1; mDIA1"
                     /note="diaphanous-related formin 1"
                     /db_xref="GeneID:307483"
                     /db_xref="RGD:1310707"
     exon            1..211
                     /gene="Diaph1"
                     /gene_synonym="Diap1; DRF1; mDIA1"
                     /inference="alignment:Splign:2.1.0"
     CDS             95..3895
                     /gene="Diaph1"
                     /gene_synonym="Diap1; DRF1; mDIA1"
                     /note="isoform 1 is encoded by transcript variant 1;
                     protein diaphanous homolog 1"
                     /codon_start=1
                     /product="protein diaphanous homolog 1 isoform 1"
                     /protein_id="NP_001100863.3"
                     /db_xref="GeneID:307483"
                     /db_xref="RGD:1310707"
                     /translation="MEPSGGGLGPGRGTRDKKKGRSPDELPATGGDGGKHKKFTLKRL
                     MADELERFTSMRIKKEKEKPNSAHRNSSASYGDDPTAQSLQDISDDQVLVLFEQMLVD
                     MNLNEEKQQPLREKDIVIKREMVSQYLHTSKAGMNQKESSRSAMMYIQELRSGLRDMH
                     LLSCLESLRVSLNNNPVSWVQTFGAEGLASLLDILKRLHDEKEETSGNYDSRNQHEII
                     RCLKAFMNNKFGIKTMLETEEGILLLVRAMDPAVPNMMIDAAKLLSALCILPQPEDMN
                     ERVLEAMTERAEMEEVERFQPLLDGLKSGTSIALKVGCLQLINALITPAEELDFRVHI
                     RSELMRLGLHQVLQELREIDNDDMRVQLNVFDEQGDEDFFDLKGRLDDIRMEMDDFGE
                     VFQIILNTVKDSKAEPHFLSILQHLLLVRNDYEARPQYYKLIEECVSQIVLHKNGTDP
                     DFKCRHLQIDIEGLVDQMIDKTKVEKSEAKATELEKKLDSELTARHELQVEMKKMEND
                     FEQKLQDLQGEKDALDSEKQQITTQKQDLEAEVSKLTGEVAKLSKELEDAKKEMASLS
                     AVAVAPSVSSSAAVPQAPPLPGDSGTVIPPPPPPPPLPGGAVPPPPPPPLPAGTGIPP
                     PPPLPGGACISSSPQLFGSTAIPPPPPLPGATAIPPPPPLPGATAIPPPPPLPGATAI
                     PPPPPLPGATGVPPPPPPLPGSVGVPPPPPLPGGPGLPPPPPPFPGAPGIPPPPPGMG
                     VPPPPPFGFGIPAAPVLPFGLTPKKVYKPEVQLRRPNWSKFVAEDLSQDCFWTKVKED
                     RFENNELFAKLTLAFSAQTKTSKAKKDQEGGEEKKSVQKKKVKELKVLDSKTAQNLSI
                     FLGSFRMPYQEIKNVILEVNEAVLTESMIQNLIKQMPEPEQLKMLSELKEEYDDLAES
                     EQFGVVMGTVPRLRPRLNAILFKLQFSEQVENIKPEIVSVTAACEELRKSENFSSLLE
                     LTLLVGNYMNAGSRNAGAFGFNISFLCKLRDTKSADQKMTLLHFLAELCETDHPDVLK
                     FPDELAHVEKASRVSAENLQKNLDQMKKQIADVERDVQNFPAATDEKDKFVEKMTSFV
                     KDAQEQYNKLRMMHSNMETLYKELGDYFVFDPKKLSVEEFFMDLHNFRNMFLQAVKEN
                     QKRRETEEKMRRAKLAKEKAEKERLEKQQKREQLIDMNAEGDETGVMDSLLEALQSGA
                     AFRRKRGPRQVNRKAGCAVTSLLASELTKDDAVAASSAKVPKKSEGVTTILEEAKELV
                     GRAS"
     exon            212..238
                     /gene="Diaph1"
                     /gene_synonym="Diap1; DRF1; mDIA1"
                     /inference="alignment:Splign:2.1.0"
     exon            239..394
                     /gene="Diaph1"
                     /gene_synonym="Diap1; DRF1; mDIA1"
                     /inference="alignment:Splign:2.1.0"
     exon            395..496
                     /gene="Diaph1"
                     /gene_synonym="Diap1; DRF1; mDIA1"
                     /inference="alignment:Splign:2.1.0"
     exon            497..627
                     /gene="Diaph1"
                     /gene_synonym="Diap1; DRF1; mDIA1"
                     /inference="alignment:Splign:2.1.0"
     exon            628..714
                     /gene="Diaph1"
                     /gene_synonym="Diap1; DRF1; mDIA1"
                     /inference="alignment:Splign:2.1.0"
     exon            715..778
                     /gene="Diaph1"
                     /gene_synonym="Diap1; DRF1; mDIA1"
                     /inference="alignment:Splign:2.1.0"
     exon            779..918
                     /gene="Diaph1"
                     /gene_synonym="Diap1; DRF1; mDIA1"
                     /inference="alignment:Splign:2.1.0"
     exon            919..1027
                     /gene="Diaph1"
                     /gene_synonym="Diap1; DRF1; mDIA1"
                     /inference="alignment:Splign:2.1.0"
     exon            1028..1138
                     /gene="Diaph1"
                     /gene_synonym="Diap1; DRF1; mDIA1"
                     /inference="alignment:Splign:2.1.0"
     exon            1139..1257
                     /gene="Diaph1"
                     /gene_synonym="Diap1; DRF1; mDIA1"
                     /inference="alignment:Splign:2.1.0"
     exon            1258..1374
                     /gene="Diaph1"
                     /gene_synonym="Diap1; DRF1; mDIA1"
                     /inference="alignment:Splign:2.1.0"
     exon            1375..1490
                     /gene="Diaph1"
                     /gene_synonym="Diap1; DRF1; mDIA1"
                     /inference="alignment:Splign:2.1.0"
     exon            1491..1555
                     /gene="Diaph1"
                     /gene_synonym="Diap1; DRF1; mDIA1"
                     /inference="alignment:Splign:2.1.0"
     exon            1556..1735
                     /gene="Diaph1"
                     /gene_synonym="Diap1; DRF1; mDIA1"
                     /inference="alignment:Splign:2.1.0"
     exon            1736..2434
                     /gene="Diaph1"
                     /gene_synonym="Diap1; DRF1; mDIA1"
                     /inference="alignment:Splign:2.1.0"
     exon            2435..2549
                     /gene="Diaph1"
                     /gene_synonym="Diap1; DRF1; mDIA1"
                     /inference="alignment:Splign:2.1.0"
     exon            2550..2558
                     /gene="Diaph1"
                     /gene_synonym="Diap1; DRF1; mDIA1"
                     /inference="alignment:Splign:2.1.0"
     exon            2559..2657
                     /gene="Diaph1"
                     /gene_synonym="Diap1; DRF1; mDIA1"
                     /inference="alignment:Splign:2.1.0"
     exon            2658..2752
                     /gene="Diaph1"
                     /gene_synonym="Diap1; DRF1; mDIA1"
                     /inference="alignment:Splign:2.1.0"
     exon            2753..2854
                     /gene="Diaph1"
                     /gene_synonym="Diap1; DRF1; mDIA1"
                     /inference="alignment:Splign:2.1.0"
     exon            2855..3094
                     /gene="Diaph1"
                     /gene_synonym="Diap1; DRF1; mDIA1"
                     /inference="alignment:Splign:2.1.0"
     exon            3095..3224
                     /gene="Diaph1"
                     /gene_synonym="Diap1; DRF1; mDIA1"
                     /inference="alignment:Splign:2.1.0"
     exon            3225..3349
                     /gene="Diaph1"
                     /gene_synonym="Diap1; DRF1; mDIA1"
                     /inference="alignment:Splign:2.1.0"
     exon            3350..3514
                     /gene="Diaph1"
                     /gene_synonym="Diap1; DRF1; mDIA1"
                     /inference="alignment:Splign:2.1.0"
     exon            3515..3650
                     /gene="Diaph1"
                     /gene_synonym="Diap1; DRF1; mDIA1"
                     /inference="alignment:Splign:2.1.0"
     exon            3651..3737
                     /gene="Diaph1"
                     /gene_synonym="Diap1; DRF1; mDIA1"
                     /inference="alignment:Splign:2.1.0"
     exon            3738..5617
                     /gene="Diaph1"
                     /gene_synonym="Diap1; DRF1; mDIA1"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 agaaggctgc tgggcggcgg cggtggttgt tggctcgggg cagccgggcg ggagcggcgt
       61 agacaagggg tctctggccg gcgctaacca ggacatggag ccgtccggcg ggggcctagg
      121 gcccggccgc gggacccggg acaagaagaa gggtcggagc ccggatgagc tgcctgcgac
      181 gggcggcgac ggcggcaaac ataagaaatt tactttgaag cggctcatgg cagacgagct
      241 ggagagattt accagcatga ggattaagaa ggagaaggaa aagcccaatt ctgctcatag
      301 aaactcctct gcatcgtatg gagatgaccc cactgctcag tcactgcagg acatctcaga
      361 tgatcaagtt cttgtcctct ttgagcagat gctggtggat atgaacctga atgaggagaa
      421 acagcagcct ttgcgagaga aggacattgt catcaagagg gagatggtgt cccagtatct
      481 gcatacttcc aaagctggca tgaaccagaa ggagagttcc aggtctgcca tgatgtacat
      541 tcaggagctg aggtcaggct tgcgggatat gcacctgctt agctgcctgg agtcccttcg
      601 agtctctctc aacaataacc ctgtcagttg ggtgcagaca tttggtgctg agggcctagc
      661 ctccttattg gacatcctca aacgactcca tgatgagaaa gaggagactt ctggaaacta
      721 cgacagccgg aaccagcatg agattatccg ctgtttgaaa gctttcatga acaacaagtt
      781 tggaatcaaa accatgttgg agacggaaga aggaatccta ctgctggtca gagccatgga
      841 tcctgctgtt cccaatatga tgattgatgc agcaaagctg ctgtctgccc tctgtatcct
      901 accgcagcca gaggacatga atgaacgagt tttagaggct atgacagaga gagctgaaat
      961 ggaagaggtt gaacgcttcc agccactgct ggatgggtta aaaagtggga cgtctattgc
     1021 actcaaagtg ggatgcctac agctgatcaa tgctctcatc actcccgctg aggaacttga
     1081 cttccgggtt cacatccgaa gtgagctgat gcgcttggga ctgcatcaag tgttgcagga
     1141 gcttcgagag attgacaatg atgatatgcg agtacagctc aatgtgtttg atgaacaagg
     1201 ggatgaagat ttcttcgatc tgaagggacg actggatgat atccgcatgg agatggatga
     1261 ctttggtgaa gttttccaga ttattttaaa cacagtgaaa gattccaagg cagaaccaca
     1321 cttcctgtct atcttgcagc atctcctgtt ggtccgaaat gattatgaag ctaggccgca
     1381 gtactataaa ttgattgaag aatgtgtttc tcagatagtt ctacacaaaa atggaactga
     1441 tcctgacttc aagtgccggc acctgcagat tgatattgag gggttggttg atcaaatgat
     1501 tgataaaacg aaggtggaaa aatctgaggc caaagctaca gagctggaaa aaaagttgga
     1561 ctcagaatta acagcccggc atgagttaca agtagaaatg aaaaagatgg aaaatgactt
     1621 tgagcagaaa cttcaggatc ttcaaggaga aaaggatgcc ttggattctg aaaagcagca
     1681 gatcactaca caaaaacaag acctggaagc agaggtgtcc aagctgacag gagaggttgc
     1741 caagctgtca aaagaactag aagatgccaa gaaggaaatg gcttctctct ctgctgtggc
     1801 tgttgcacct tcagtttcta gtagtgctgc tgttccccaa gcccctcctc tgcctggtga
     1861 ctcaggcact gttattccac ctcccccacc cccaccccct ctccctggag gtgccgtccc
     1921 accccctcct cctcctcctc tgcctgcagg tactggtatc cctccacctc ctcctttacc
     1981 tggaggtgct tgtatatcct cttccccaca gttgtttggc agtactgcca ttcccccacc
     2041 tcctcctttg cctggagcta ctgccatccc cccacctccc cctttgcctg gagctactgc
     2101 catcccccca cctccccctt tgcctggagc tactgccatc ccccctcctc ctcctttgcc
     2161 tggagctact ggtgtaccac cacctcctcc ccctttacct ggaagtgttg gtgttccccc
     2221 accccctccc ttgcctggag gaccaggact gcctcctcct cctcccccct ttcctggagc
     2281 acctggcatt ccccccccac cgcctggtat gggcgtgcct ccacctcccc cctttggatt
     2341 tgggattcct gcagccccag ttctgccatt tggattaacc cccaaaaaag tttataagcc
     2401 agaggtgcaa ctccggaggc caaactggtc caagttcgtg gctgaggacc tttcccagga
     2461 ctgcttctgg acaaaggtga aggaggaccg ctttgagaac aatgaactat ttgccaaact
     2521 tacccttgcc ttctctgccc agaccaagac ttctaaagcc aagaaggatc aagaaggtgg
     2581 agaagaaaag aaatctgttc aaaagaagaa agtaaaagag ctgaaagtgc tggattcaaa
     2641 gacagcccag aatctctcaa tctttttggg ttcattccgt atgccctatc aagagattaa
     2701 gaacgttatc ctggaggtga atgaggctgt tctcacggag tctatgatcc agaacctcat
     2761 taagcagatg ccagaaccag agcagttaaa aatgctttct gaactgaagg aggagtacga
     2821 tgatctggct gagtcagagc agttcggcgt ggtgatgggc acggttcccc gtcttcggcc
     2881 tcgcctcaat gccatccttt tcaagctaca gttcagtgag caagttgaga atatcaagcc
     2941 agagattgta tctgtcactg cggcatgtga ggagctgcgt aagagtgaga acttctctag
     3001 cctcctggag ctcacactgc tcgttggaaa ctatatgaat gcgggctcca ggaatgctgg
     3061 agcttttggc ttcaatatca gcttcctttg taagcttcga gacaccaagt ctgcagatca
     3121 gaagatgact ctgttgcatt tcttggctga gttatgtgag actgaccacc ccgatgtcct
     3181 caagtttcct gatgagcttg cccatgtaga gaaagccagc agagtttctg ctgagaactt
     3241 gcagaagaat ttagatcaga tgaagaaaca gattgctgat gtggaacgcg atgttcaaaa
     3301 tttcccagct gccacagacg aaaaagacaa gtttgttgag aagatgacta gctttgtgaa
     3361 ggatgcacag gaacagtata acaagctacg gatgatgcac tccaacatgg agactctcta
     3421 taaggagcta ggcgactact tcgtctttga ccctaagaag ttgtctgtag aagaattttt
     3481 tatggatctg cacaacttta ggaatatgtt tttgcaagca gtcaaggaga accagaagcg
     3541 ccgggaaaca gaagaaaaga tgaggagagc aaaactagcc aaggaaaagg cggaaaaaga
     3601 gcgactggag aagcagcaga agcgagagca gctcatagac atgaatgcag agggggatga
     3661 gacaggtgtg atggacagtc ttctagaagc tctgcagtca ggagcagcat tccgacggaa
     3721 gagagggccc cggcaggtca acaggaaagc tgggtgtgca gtcacatctc tgctagcctc
     3781 ggagctgacc aaggatgatg ccgtggctgc tagttctgct aaggtgccca agaaaagtga
     3841 aggagtcacc acaatccttg aagaagccaa ggaactggtt ggccgtgcaa gctaagctgg
     3901 gctttatggc cgttgctgca tctaggtgaa gcccagactg cctacctgca gcatgtgcct
     3961 aaatggtcaa ggagatatcc ctctgcagtg gccgctccac cacctgaccc tgtctctctg
     4021 tctggcctgc tgctctcgaa caccacatac agcttcagct gcctggaggc cagaaggaag
     4081 aggcagtgta ggggtggcct gagcccagcc cagccagccc tggctgttgt attaccaaag
     4141 cagggtccgt gtttgctgcc ttaaccctgt ctcctctatg ttacccagag gtcctggtct
     4201 cagacagaac ccagcctgct ttctcagccc cactctctaa tgggccttcc cctaggtcaa
     4261 tcttgctgca tttgtgcttt tcttttgtgg tttctctggc cctgagaata gcatgggact
     4321 tgtgaacctt cgggctagat cttttcactg ctgtcactca ctctgctttt cctgctggcg
     4381 gttatttatt attagtgctg tggcattggg agctgcttct gcaaaaacag ggagcagatc
     4441 ccacccttac cccaccttcc tgggaaaggt ctcccgggaa aagggtctca accactctcc
     4501 ccgttccact gtggcccagg tctgagccat aggcaggtag ctagggcata gcgacagtgt
     4561 ggggtccgcc ccctgcccgc tgccatgctt catgcccttg cccccctggt ccctctgcac
     4621 cagcctcacc tctacccagc aactgaaccc ttccccccca tgccttcccc agagctttgg
     4681 tgcatctcat ttggagtgtg ggctgtagtg tgaccatccc aacacctcac ccccaccatc
     4741 caaggaaatg ggaggtggag cctcctggct gagaaattgt tttgcaaatg gatctatttt
     4801 tatatgaaaa attttttaag aaaaataact ttgatcccct ttcccctgcg taataaaaga
     4861 ggctttgatg gtaggttcaa gcattcctgg ggtcagtcct gaacatggcc taaatattgg
     4921 ccccacccct caagactaaa tggtgaggcc aaggcagaca gacccttcct tagcatcagt
     4981 tctggggcaa gagaaattcc tctgggtggg ctgggccgca ccatagggta gcaagagctc
     5041 agatgctgtg ttttcatgaa tgtattaaat tatcagccaa atgtgcagat gctaaggatg
     5101 ctgagaagtg cacaggggtg cccccatata agcagtgagc cagactgggt ggcccaaacc
     5161 tcaggaagct tagactctct ggcctgggag tcactgtctt gtcctcctcc agggcccttt
     5221 cagcttccct ctgaggctac agagaaggct tgacacagaa ttggagttgc cgagtatgaa
     5281 ggggttagga aacacaaggg ccagtacagg ggccttgtgc ccagggagga ttggttgggt
     5341 tgctcgagct tgtgtctgag ggacagtggc agcttctaga ctacttcctg ggaggactga
     5401 agagcccgtt cttcctgagc caccaccttg ctcgctgaca tctggggctt tgaccctttt
     5461 ctttttttaa tcacttttgc taaaatgcat ttaattaaaa aaatttttaa gtagaggggg
     5521 taaaatgcaa acccccttgc ctcttgggct tttgggatgt catcacctcc agtttgatgg
     5581 gtttgtttcc aactgtcaat aaagcattga aacagaa
//
