LOCUS       NM_001413227            4245 bp    mRNA    linear   ROD 31-MAR-2024
DEFINITION  Rattus norvegicus DEAH-box helicase 8 (Dhx8), mRNA.
ACCESSION   NM_001413227 XM_006247286
VERSION     NM_001413227.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 4245)
  AUTHORS   Bertram,K., Agafonov,D.E., Liu,W.T., Dybkov,O., Will,C.L.,
            Hartmuth,K., Urlaub,H., Kastner,B., Stark,H. and Luhrmann,R.
  TITLE     Cryo-EM structure of a human spliceosome activated for step 2 of
            splicing
  JOURNAL   Nature 542 (7641), 318-323 (2017)
   PUBMED   28076346
REFERENCE   2  (bases 1 to 4245)
  AUTHORS   Chang,T.H., Tung,L., Yeh,F.L., Chen,J.H. and Chang,S.L.
  TITLE     Functions of the DExD/H-box proteins in nuclear pre-mRNA splicing
  JOURNAL   Biochim Biophys Acta 1829 (8), 764-774 (2013)
   PUBMED   23454554
  REMARK    Review article
REFERENCE   3  (bases 1 to 4245)
  AUTHORS   Baltz,A.G., Munschauer,M., Schwanhausser,B., Vasile,A.,
            Murakawa,Y., Schueler,M., Youngs,N., Penfold-Brown,D., Drew,K.,
            Milek,M., Wyler,E., Bonneau,R., Selbach,M., Dieterich,C. and
            Landthaler,M.
  TITLE     The mRNA-bound proteome and its global occupancy profile on
            protein-coding transcripts
  JOURNAL   Mol Cell 46 (5), 674-690 (2012)
   PUBMED   22681889
REFERENCE   4  (bases 1 to 4245)
  AUTHORS   Castello,A., Fischer,B., Eichelbaum,K., Horos,R., Beckmann,B.M.,
            Strein,C., Davey,N.E., Humphreys,D.T., Preiss,T., Steinmetz,L.M.,
            Krijgsveld,J. and Hentze,M.W.
  TITLE     Insights into RNA biology from an atlas of mammalian mRNA-binding
            proteins
  JOURNAL   Cell 149 (6), 1393-1406 (2012)
   PUBMED   22658674
REFERENCE   5  (bases 1 to 4245)
  AUTHORS   Hegele,A., Kamburov,A., Grossmann,A., Sourlis,C., Wowro,S.,
            Weimann,M., Will,C.L., Pena,V., Luhrmann,R. and Stelzl,U.
  TITLE     Dynamic protein-protein interaction wiring of the human spliceosome
  JOURNAL   Mol Cell 45 (4), 567-580 (2012)
   PUBMED   22365833
REFERENCE   6  (bases 1 to 4245)
  AUTHORS   Jurica,M.S., Licklider,L.J., Gygi,S.R., Grigorieff,N. and
            Moore,M.J.
  TITLE     Purification and characterization of native spliceosomes suitable
            for three-dimensional structural analysis
  JOURNAL   RNA 8 (4), 426-439 (2002)
   PUBMED   11991638
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            JAXUCZ010000010.1.
            
            On Oct 27, 2022 this sequence version replaced XM_006247286.4.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR26360181.1803439.1,
                                           SRR22582416.1075966.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA5760383, SAMEA5760386
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-160               JAXUCZ010000010.1  87168093-87168252
            161-246             JAXUCZ010000010.1  87175034-87175119
            247-319             JAXUCZ010000010.1  87175840-87175912
            320-405             JAXUCZ010000010.1  87176276-87176361
            406-515             JAXUCZ010000010.1  87178038-87178147
            516-941             JAXUCZ010000010.1  87178619-87179044
            942-1086            JAXUCZ010000010.1  87180075-87180219
            1087-1290           JAXUCZ010000010.1  87180310-87180513
            1291-1378           JAXUCZ010000010.1  87181100-87181187
            1379-1476           JAXUCZ010000010.1  87183191-87183288
            1477-1624           JAXUCZ010000010.1  87184955-87185102
            1625-1806           JAXUCZ010000010.1  87188239-87188420
            1807-2001           JAXUCZ010000010.1  87189444-87189638
            2002-2187           JAXUCZ010000010.1  87190467-87190652
            2188-2398           JAXUCZ010000010.1  87190741-87190951
            2399-2580           JAXUCZ010000010.1  87191659-87191840
            2581-2721           JAXUCZ010000010.1  87193217-87193357
            2722-2877           JAXUCZ010000010.1  87196062-87196217
            2878-3015           JAXUCZ010000010.1  87199674-87199811
            3016-3144           JAXUCZ010000010.1  87201111-87201239
            3145-3341           JAXUCZ010000010.1  87201392-87201588
            3342-3521           JAXUCZ010000010.1  87202034-87202213
            3522-4245           JAXUCZ010000010.1  87203703-87204426
FEATURES             Location/Qualifiers
     source          1..4245
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="BN"
                     /db_xref="taxon:10116"
                     /chromosome="10"
                     /map="10q32.1"
     gene            1..4245
                     /gene="Dhx8"
                     /gene_synonym="LRRGT00035"
                     /note="DEAH-box helicase 8"
                     /db_xref="GeneID:287727"
                     /db_xref="RGD:1310723"
     exon            1..160
                     /gene="Dhx8"
                     /gene_synonym="LRRGT00035"
                     /inference="alignment:Splign:2.1.0"
     CDS             13..3741
                     /gene="Dhx8"
                     /gene_synonym="LRRGT00035"
                     /EC_number="3.6.1.-"
                     /note="DEAH (Asp-Glu-Ala-His) box polypeptide 8"
                     /codon_start=1
                     /product="ATP-dependent RNA helicase DHX8"
                     /protein_id="NP_001400156.1"
                     /db_xref="GeneID:287727"
                     /db_xref="RGD:1310723"
                     /translation="MAVAVATAGVLMGSEPGPAEELAKLEYLSLVSKVCTELDNHLGI
                     NDKDLAEFVISLAEKNTTFDTFKASLVKNGAEFTDSLISNLLRLIQTMRPPAKPSTSK
                     DPVVKPKTEKEKLKELFPVLCQPDNPSVRTMLDEEDVKVAVDVLKELEALMPSAAGQE
                     KQRDTEHRDRTKKKKRSRSRDRDRDRDRDRDRDRDRDRDKDRERDRDRERDRERDRDR
                     DHKRRHRSRSRSHSRTRERAKGKSRYRSRSRSQSPFKDRKDREKYGERNLDRWRDKHV
                     DRPPPEEPAIGDIYNGKVTSIMQFGCFVQLEGLRKRWEGLVHISELRREGRVANVADV
                     VSKGQRVKVKVLSFTGTKTSLSMKDVDQETGEDLNPNRRRNLVGETNEETSMRNPDRP
                     THLSLVSAPEVEDDSLERKRLTRISDPEKWEIKQMIAANVLSKEEFPDFDEETGILPK
                     VDDEEDEDLEIELVEEEPPFLRGHTKQSMDMSPIKIVKNPDGSLSQAAMMQSALAKER
                     RELKQAQREAEMDSIPMGLNKHWVDPLPDAEGRQIAANMRGIGMMPNDIPEWKKHAFG
                     GNKASYGKKTQMSILEQRESLPIYKLKEQLVQAVHDNQILIVIGETGSGKTTQITQYL
                     AEAGYTSRGKIGCTQPRRVAAMSVAKRVSEEFGCCLGQEVGYTIRFEDCTSPETVIKY
                     MTDGMLLRECLIDPDLTQYAIIMLDEAHERTIHTDVLFGLLKKTVQKRQDMKLIVTSA
                     TLDAVKFSQYFYEAPIFTIPGRTYPVEILYTKEPETDYLDASLITVMQIHLTEPPGDI
                     LVFLTGQEEIDTACEILYERMKSLGPDVPELIILPVYSALPSEMQTRIFDPAPPGSRK
                     VVIATNIAETSLTIDGIYYVVDPGFVKQKVYNSKTGIDQLVVTPISQAQAKQRAGRAG
                     RTGPGKCYRLYTERAYRDEMLTTNVPEIQRTNLASTVLSLKAMGINDLLSFDFMDAPP
                     METLITAMEQLYTLGALDDEGLLTRLGRRMAEFPLEPMLCKMLIMSVHLGCSEEMLTI
                     VSMLSVQNVFYRPKDKQALADQKKAKFHQTEGDHLTLLAVYNSWKNNKFSNPWCYENF
                     IQARSLRRAQDIRKQMLGIMDRHKLDVVSCGKSTVRVQKAICSGFFRNAAKKDPQEGY
                     RTLIDQQVVYIHPSSALFNRQPEWVVYHELVLTTKEYMREVTTIDPRWLVEFAPAFFK
                     VSDPTKLSKQKKQQRLEPLYNRYEEPNAWRISRAFRRR"
     exon            161..246
                     /gene="Dhx8"
                     /gene_synonym="LRRGT00035"
                     /inference="alignment:Splign:2.1.0"
     exon            247..319
                     /gene="Dhx8"
                     /gene_synonym="LRRGT00035"
                     /inference="alignment:Splign:2.1.0"
     exon            320..405
                     /gene="Dhx8"
                     /gene_synonym="LRRGT00035"
                     /inference="alignment:Splign:2.1.0"
     exon            406..515
                     /gene="Dhx8"
                     /gene_synonym="LRRGT00035"
                     /inference="alignment:Splign:2.1.0"
     exon            516..941
                     /gene="Dhx8"
                     /gene_synonym="LRRGT00035"
                     /inference="alignment:Splign:2.1.0"
     exon            942..1086
                     /gene="Dhx8"
                     /gene_synonym="LRRGT00035"
                     /inference="alignment:Splign:2.1.0"
     exon            1087..1290
                     /gene="Dhx8"
                     /gene_synonym="LRRGT00035"
                     /inference="alignment:Splign:2.1.0"
     exon            1291..1378
                     /gene="Dhx8"
                     /gene_synonym="LRRGT00035"
                     /inference="alignment:Splign:2.1.0"
     exon            1379..1476
                     /gene="Dhx8"
                     /gene_synonym="LRRGT00035"
                     /inference="alignment:Splign:2.1.0"
     exon            1477..1624
                     /gene="Dhx8"
                     /gene_synonym="LRRGT00035"
                     /inference="alignment:Splign:2.1.0"
     exon            1625..1806
                     /gene="Dhx8"
                     /gene_synonym="LRRGT00035"
                     /inference="alignment:Splign:2.1.0"
     exon            1807..2001
                     /gene="Dhx8"
                     /gene_synonym="LRRGT00035"
                     /inference="alignment:Splign:2.1.0"
     exon            2002..2187
                     /gene="Dhx8"
                     /gene_synonym="LRRGT00035"
                     /inference="alignment:Splign:2.1.0"
     exon            2188..2398
                     /gene="Dhx8"
                     /gene_synonym="LRRGT00035"
                     /inference="alignment:Splign:2.1.0"
     exon            2399..2580
                     /gene="Dhx8"
                     /gene_synonym="LRRGT00035"
                     /inference="alignment:Splign:2.1.0"
     exon            2581..2721
                     /gene="Dhx8"
                     /gene_synonym="LRRGT00035"
                     /inference="alignment:Splign:2.1.0"
     exon            2722..2877
                     /gene="Dhx8"
                     /gene_synonym="LRRGT00035"
                     /inference="alignment:Splign:2.1.0"
     exon            2878..3015
                     /gene="Dhx8"
                     /gene_synonym="LRRGT00035"
                     /inference="alignment:Splign:2.1.0"
     exon            3016..3144
                     /gene="Dhx8"
                     /gene_synonym="LRRGT00035"
                     /inference="alignment:Splign:2.1.0"
     exon            3145..3341
                     /gene="Dhx8"
                     /gene_synonym="LRRGT00035"
                     /inference="alignment:Splign:2.1.0"
     exon            3342..3521
                     /gene="Dhx8"
                     /gene_synonym="LRRGT00035"
                     /inference="alignment:Splign:2.1.0"
     exon            3522..4245
                     /gene="Dhx8"
                     /gene_synonym="LRRGT00035"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 gaagttgtag ccatggctgt ggccgtggcc accgccgggg tcctaatggg ctcggaacct
       61 ggcccggcgg aagagctcgc taaactcgag tacctgtctt tggtgtcaaa ggtttgcacg
      121 gagctggaca atcatttggg catcaacgac aaggatctgg ctgaatttgt catcagtctt
      181 gctgagaaaa atactacctt tgatactttt aaggcttctc tggtcaaaaa tggtgcagaa
      241 ttcacagatt ctcttattag caacctgttg cgtctcatac aaactatgcg gcctccagca
      301 aagccttcta ctagtaaaga tcccgttgtt aaacccaaaa ctgaaaaaga aaagctgaag
      361 gaactcttcc ctgtcctttg ccagccagac aacccttcag ttcggaccat gttggatgaa
      421 gaagatgtga aagttgctgt ggatgttttg aaagagctgg aagctttaat gcccagtgca
      481 gcaggccaag agaaacaaag agacactgag cacagggaca ggacaaagaa gaagaagagg
      541 agtcggagcc gagatcgtga tcgtgatcga gaccgggaca gggaccgaga ccgggacagg
      601 gaccgtgaca aggaccgaga acgagataga gatcgagaac gagacagaga gcgagataga
      661 gatagagacc ataagcggag acaccgatca cgctctcggt cacattctag gacccgggag
      721 agggctaagg ggaaatctag atacaggtct aggagcagaa gtcagagtcc cttcaaagat
      781 cggaaagacc gggaaaagta tggggagagg aatctggaca gatggcggga taagcatgtg
      841 gaccgccctc ctcctgaaga gcctgccatt ggagacattt acaatggcaa agtgaccagc
      901 atcatgcagt ttggctgctt cgtgcagctg gaaggcctaa ggaagcggtg ggaaggcctg
      961 gtgcacattt ctgagcttcg acgggaaggt cgagtggcca atgtggctga tgttgtgagc
     1021 aaaggccaga gggttaaggt caaagttctg tccttcactg ggaccaagac cagcctgagc
     1081 atgaaggatg tggatcaaga aacaggagaa gatctaaacc caaacagacg cagaaatctg
     1141 gttggggaga ccaacgaaga gacgtcgatg cggaacccag accgacccac ccacctctcc
     1201 ctcgtcagtg ctcctgaagt agaggacgac tcactggagc gcaagcggct cacccgcatc
     1261 tctgacccag agaagtggga gatcaaacag atgattgctg ccaatgtgct ttccaaagaa
     1321 gagtttccag acttcgatga agagaccggc attctcccta aggtggatga tgaagaagat
     1381 gaggatcttg aaattgaact tgttgaagag gagcctccgt tcctgagagg gcacaccaag
     1441 cagagcatgg acatgagccc catcaaaatc gttaagaacc cggatggctc cctctcccaa
     1501 gcagccatga tgcagagtgc cctggccaaa gaaaggcggg agctgaagca ggcccagcgg
     1561 gaagcagaga tggattctat acccatgggg ctcaacaaac actgggtgga ccccttgcct
     1621 gatgcggaag gcaggcagat cgctgccaac atgaggggga ttgggatgat gcccaatgac
     1681 attcctgagt ggaagaagca tgcctttggg ggcaacaaag cttcttacgg gaagaagacc
     1741 cagatgtcaa tccttgagca gagagagagc ctgcctatct acaaactgaa ggaacagctg
     1801 gtgcaggctg tacatgacaa tcagatccta attgtaatcg gagagacagg atctgggaag
     1861 acaacacaga tcacccagta cctggcagag gcaggctata cttccagggg caagattggg
     1921 tgcacccagc ccagaagagt ggcagctatg tctgtggcca aaagagtatc agaggaattt
     1981 ggttgttgcc ttggtcaaga ggtaggctac accattcgat ttgaggattg caccagccca
     2041 gaaacagtca tcaagtacat gacagacggc atgctgctca gagagtgcct gattgatccc
     2101 gatctcactc agtacgccat catcatgctg gacgaggcac atgagaggac tattcacaca
     2161 gatgtgctct ttgggttgtt gaaaaagaca gttcaaaaac gacaagacat gaagctgatt
     2221 gtcacctcag ccaccctgga tgcagtgaag ttttctcagt acttctatga agcacccatc
     2281 ttcacaatcc ctggtcgaac ctatccagtg gagattctgt acacaaagga gcccgaaaca
     2341 gactacttgg acgccagcct gatcacagtc atgcagatcc acctgacaga gccgccaggt
     2401 gacatcttgg tttttttgac tggtcaggaa gagatcgata ctgcttgtga gatcctgtat
     2461 gaaagaatga agtcactggg acctgacgtc ccagagttga tcatcctccc agtgtactct
     2521 gctcttccga gtgagatgca gacccgaatc tttgatccag ctcctcctgg cagcagaaag
     2581 gtggtgattg ccaccaacat tgcagaaaca tctctgacca tcgacggcat ctactatgtg
     2641 gtcgaccctg gatttgtgaa gcaaaaagtt tacaattcta aaacagggat tgaccagctc
     2701 gtggtgacac ctatttctca ggctcaagca aagcagagag ctggcagagc tgggagaacc
     2761 ggcccaggca agtgttacag gctgtacaca gagcgtgcct accgggatga aatgctgacc
     2821 accaatgtgc cagaaatcca gagaaccaac ctggccagca cagtgctgtc cctcaaggcc
     2881 atgggcatta atgacctgct gtcctttgac ttcatggatg cgccaccgat ggaaactctg
     2941 atcacagcca tggagcaact gtatacgctg ggggccctgg acgatgaggg cctgctcact
     3001 cgccttggcc gcaggatggc agagttccct ctggagccaa tgctctgcaa aatgctcatc
     3061 atgtctgtgc atctgggctg cagcgaagag atgctaacta ttgtgtccat gctgtctgtg
     3121 cagaacgtct tctaccgacc caaggacaag caggcccttg cagatcagaa gaaggccaaa
     3181 ttccaccaga cagaagggga ccatctcacc ctgctggccg tgtacaattc ctggaagaac
     3241 aacaaattct ccaacccatg gtgctatgag aacttcatcc aggctcgttc cttacgccgg
     3301 gcccaggaca ttcgaaagca gatgttaggc ataatggaca ggcacaagct ggatgtggtc
     3361 tcctgtggta aatccacagt gcgagtgcag aaggccatct gcagtggctt cttccggaat
     3421 gctgccaaga aggaccccca ggagggctac cggacattga ttgaccagca ggtggtctat
     3481 atccatcctt ccagtgctct tttcaacaga cagcccgaat gggtggtata ccatgaactg
     3541 gtgctgacca caaaggagta catgcgtgag gtcaccacca tcgacccccg gtggcttgtg
     3601 gagtttgctc cggctttctt caaagtctct gacccaacca agctaagcaa gcagaagaag
     3661 cagcagcgcc tggagcctct gtacaatcgg tatgaggaac cgaacgcctg gagaatctcc
     3721 cgtgctttcc ggcggcgctg aaagacagct gaccactcgc ctctcctgca ttgtggcttc
     3781 ctctggctga taataagcta gtcctgtggt gacttaatct taaactgagc attttccaag
     3841 cctgactctc tccatagtgt aagacagaaa catctaagca ggacttcggc cagagccttt
     3901 aatcccagca cttgggacac agaggcagga agatctcttg agtttgaggc caacctggtc
     3961 tacagagcaa gctccaggat agccagggct accaagagaa accctgtctt gaaaaaaaaa
     4021 tcacacacac acaaaagaac aattagataa gttgtgtatc ctgggatggg cgaggggcac
     4081 gcgtgactgt gatgcctggc gggcccagcc agggccccat accccagctc atgctgaagg
     4141 agtgaaccat gactctcatg taccatgttg gccccactcc ctaccactcc ctggcctctg
     4201 tacttgggtt tctacctcct agaaatattt atttttttaa ggaaa
//
