LOCUS       NM_001025720            4280 bp    mRNA    linear   ROD 03-APR-2024
DEFINITION  Rattus norvegicus dehydrogenase E1 and transketolase domain
            containing 1 (Dhtkd1), mRNA.
ACCESSION   NM_001025720 XM_341557
VERSION     NM_001025720.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 4280)
  AUTHORS   Artiukhov,A.V., Grabarska,A., Gumbarewicz,E., Aleshin,V.A.,
            Kahne,T., Obata,T., Kazantsev,A.V., Lukashev,N.V., Stepulak,A.,
            Fernie,A.R. and Bunik,V.I.
  TITLE     Synthetic analogues of 2-oxo acids discriminate metabolic
            contribution of the 2-oxoglutarate and 2-oxoadipate dehydrogenases
            in mammalian cells and tissues
  JOURNAL   Sci Rep 10 (1), 1886 (2020)
   PUBMED   32024885
  REMARK    GeneRIF: Synthetic analogues of 2-oxo acids discriminate metabolic
            contribution of the 2-oxoglutarate and 2-oxoadipate dehydrogenases
            in mammalian cells and tissues.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4280)
  AUTHORS   Tsepkova,P.M., Artiukhov,A.V., Boyko,A.I., Aleshin,V.A.,
            Mkrtchyan,G.V., Zvyagintseva,M.A., Ryabov,S.I., Ksenofontov,A.L.,
            Baratova,L.A., Graf,A.V. and Bunik,V.I.
  TITLE     Thiamine Induces Long-Term Changes in Amino Acid Profiles and
            Activities of 2-Oxoglutarate and 2-Oxoadipate Dehydrogenases in Rat
            Brain
  JOURNAL   Biochemistry (Mosc) 82 (6), 723-736 (2017)
   PUBMED   28601082
  REMARK    GeneRIF: Thiamine Induces Long-Term Changes in Amino Acid Profiles
            and Activities of 2-Oxoglutarate and 2-Oxoadipate Dehydrogenases in
            Rat Brain
REFERENCE   3  (bases 1 to 4280)
  AUTHORS   Nagamachi,A., Matsui,H., Asou,H., Ozaki,Y., Aki,D., Kanai,A.,
            Takubo,K., Suda,T., Nakamura,T., Wolff,L., Honda,H. and Inaba,T.
  TITLE     Haploinsufficiency of SAMD9L, an endosome fusion facilitator,
            causes myeloid malignancies in mice mimicking human diseases with
            monosomy 7
  JOURNAL   Cancer Cell 24 (3), 305-317 (2013)
   PUBMED   24029230
REFERENCE   4  (bases 1 to 4280)
  AUTHORS   Xu,W.Y., Gu,M.M., Sun,L.H., Guo,W.T., Zhu,H.B., Ma,J.F., Yuan,W.T.,
            Kuang,Y., Ji,B.J., Wu,X.L., Chen,Y., Zhang,H.X., Sun,F.T.,
            Huang,W., Huang,L., Chen,S.D. and Wang,Z.G.
  TITLE     A nonsense mutation in DHTKD1 causes Charcot-Marie-Tooth disease
            type 2 in a large Chinese pedigree
  JOURNAL   Am J Hum Genet 91 (6), 1088-1094 (2012)
   PUBMED   23141294
COMMENT     PROVISIONAL REFSEQ: This record has not yet been subject to final
            NCBI review. The reference sequence was derived from BC099075.1.
            
            On Jul 22, 2005 this sequence version replaced XM_341557.2.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC099075.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA5756307, SAMEA5760383
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            gene product(s) localized to mito. :: inferred from homology
            RefSeq Select criteria             :: based on conservation,
                                                  expression, longest protein
            ##RefSeq-Attributes-END##
FEATURES             Location/Qualifiers
     source          1..4280
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /db_xref="taxon:10116"
                     /chromosome="17"
                     /map="17q12.3"
     gene            1..4280
                     /gene="Dhtkd1"
                     /gene_synonym="E1a; OADC-E1; OADH-E1"
                     /note="dehydrogenase E1 and transketolase domain
                     containing 1"
                     /db_xref="GeneID:361272"
                     /db_xref="RGD:1308092"
     exon            1..223
                     /gene="Dhtkd1"
                     /gene_synonym="E1a; OADC-E1; OADH-E1"
                     /inference="alignment:Splign:2.1.0"
     CDS             70..2832
                     /gene="Dhtkd1"
                     /gene_synonym="E1a; OADC-E1; OADH-E1"
                     /EC_number="1.2.4.2"
                     /note="probable 2-oxoglutarate dehydrogenase E1 component
                     DHKTD1, mitochondrial; dehydrogenase E1 and transketolase
                     domain-containing protein 1; probable 2-oxoglutarate
                     dehydrogenase E1 component DHKTD1, mitochondrial-like;
                     alpha-KADH-E1; alpha-ketoadipate dehydrogenase;
                     2-oxoadipate dehydrogenase, mitochondrial"
                     /codon_start=1
                     /product="2-oxoadipate dehydrogenase complex component E1"
                     /protein_id="NP_001020891.1"
                     /db_xref="GeneID:361272"
                     /db_xref="RGD:1308092"
                     /translation="MASATVAAAGRALRRAVPLLRRSYQTERGVYGYRPRKAGSGEPR
                     GDRARPSVDHGLARLVTVYCEHGHKAAQINPLFPGQALLDTVPEIQALVQTLQGPFTT
                     TGLLNMGKEEASLEEVLAYLNHIYCGPISIETAQLQSQEEKDWFARRFEELKKETFTT
                     EERKHLSKLLLESQEFDHFLATKFATVKRYGGEGAESMMGFFHELLKLSAYGGITDII
                     IGMPHRGRLNLLTGLLQLPPELMFRKMRGLSEFPENVAAIGDVLSHLTSSVDLDFGAH
                     RPLHVTMLPNPSHLEAINPVAVGKTRGRQQSQEDGDYSPNGSAQPGDKVICLQVHGDA
                     SFCGQGIVLETFTLSNLPHFRIGGSIHLIVNNQLGYTTPAERGRSSLYSSDIGKLVGC
                     AIIHVNGDSPEEVVRATRLAFEYQRQFRKDVIIDLLCYRQWGHNELDEPFFTNPVMYK
                     IIRARKSIPDTYAEHLIASGLMTQEEVSDIKASYYAKLNGHLANVAHYSPPAPHLQAR
                     WQGLVQPAACVTTWDTGVPLELLRFVGVKSVEVPEELQLHSHLLKMYVQSRMEKVKNG
                     TNLDWATAETLALGSLLAQGFNVRLSGQDVGRGTFSQRHAMVVCQNTDDVYIPLNHMD
                     PNQKGFLEVSNSPLSEEAVLGFEYGMSIESPKLLPLWEAQFGDFFNGAQIIFDTFISG
                     GEAKWLLQSGLVILLPHGYDGAGPDHSSCRIERFLQMCDSAEEGVDSDTVNMFVVHPT
                     TPAQYFHLLRRQMMRNFRKPLIVASPKMLLRYPVAVSTLEEMAPGTAFKPVIGDSSVD
                     PKNVKTLIFCSGKHFYALLKQRESLGAKKRDFAIIRLEELCPFPLDSLQQEMGKYKHV
                     QDIIWSQEEPQNMGPWSFVYPRFEKQLACKLRLVSRPPLPAPAVGIGTVHQQQHEAIL
                     FKTFTS"
     exon            224..379
                     /gene="Dhtkd1"
                     /gene_synonym="E1a; OADC-E1; OADH-E1"
                     /inference="alignment:Splign:2.1.0"
     exon            380..591
                     /gene="Dhtkd1"
                     /gene_synonym="E1a; OADC-E1; OADH-E1"
                     /inference="alignment:Splign:2.1.0"
     exon            592..786
                     /gene="Dhtkd1"
                     /gene_synonym="E1a; OADC-E1; OADH-E1"
                     /inference="alignment:Splign:2.1.0"
     exon            787..1056
                     /gene="Dhtkd1"
                     /gene_synonym="E1a; OADC-E1; OADH-E1"
                     /inference="alignment:Splign:2.1.0"
     exon            1057..1228
                     /gene="Dhtkd1"
                     /gene_synonym="E1a; OADC-E1; OADH-E1"
                     /inference="alignment:Splign:2.1.0"
     exon            1229..1427
                     /gene="Dhtkd1"
                     /gene_synonym="E1a; OADC-E1; OADH-E1"
                     /inference="alignment:Splign:2.1.0"
     exon            1428..1740
                     /gene="Dhtkd1"
                     /gene_synonym="E1a; OADC-E1; OADH-E1"
                     /inference="alignment:Splign:2.1.0"
     exon            1741..1825
                     /gene="Dhtkd1"
                     /gene_synonym="E1a; OADC-E1; OADH-E1"
                     /inference="alignment:Splign:2.1.0"
     exon            1826..1965
                     /gene="Dhtkd1"
                     /gene_synonym="E1a; OADC-E1; OADH-E1"
                     /inference="alignment:Splign:2.1.0"
     exon            1966..2116
                     /gene="Dhtkd1"
                     /gene_synonym="E1a; OADC-E1; OADH-E1"
                     /inference="alignment:Splign:2.1.0"
     exon            2117..2223
                     /gene="Dhtkd1"
                     /gene_synonym="E1a; OADC-E1; OADH-E1"
                     /inference="alignment:Splign:2.1.0"
     exon            2224..2388
                     /gene="Dhtkd1"
                     /gene_synonym="E1a; OADC-E1; OADH-E1"
                     /inference="alignment:Splign:2.1.0"
     exon            2389..2471
                     /gene="Dhtkd1"
                     /gene_synonym="E1a; OADC-E1; OADH-E1"
                     /inference="alignment:Splign:2.1.0"
     exon            2472..2641
                     /gene="Dhtkd1"
                     /gene_synonym="E1a; OADC-E1; OADH-E1"
                     /inference="alignment:Splign:2.1.0"
     exon            2642..2727
                     /gene="Dhtkd1"
                     /gene_synonym="E1a; OADC-E1; OADH-E1"
                     /inference="alignment:Splign:2.1.0"
     exon            2728..4257
                     /gene="Dhtkd1"
                     /gene_synonym="E1a; OADC-E1; OADH-E1"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 ccctcgccct ctgcttggtt gcagcttttt ccccccattc ttccacgcgt cttcgacccc
       61 agactgcaca tggcatcggc tactgtggca gctgcaggga gagctctccg ccgagcggtc
      121 ccgcttctca ggcgcagtta ccagacggag aggggcgtct atggataccg gcctaggaaa
      181 gccgggagcg gcgagccccg aggcgaccgt gcacgcccct cagttgacca tggccttgcc
      241 aggctggtga cagtgtattg tgagcatggc cacaaagctg cccagatcaa ccctctcttc
      301 cccggacaag ccctcctgga tactgtgcct gaaatccaag ctcttgtgca gacactccag
      361 ggacccttca ctaccacagg actgttaaac atgggcaaag aagaggcttc tcttgaggaa
      421 gtgttggcct atttgaacca tatctactgt gggccaattt ctattgaaac cgcccagctg
      481 cagagccagg aggagaaaga ctggtttgcc agaaggtttg aggaactgaa aaaggagaca
      541 tttaccacag aagagcgaaa acacctgtcg aaactactgc tagaatctca ggagtttgac
      601 cactttttgg ccaccaagtt tgccacagtg aagagatacg gaggtgaagg ggcagagagc
      661 atgatgggct tttttcacga gttgctgaaa ttgtcagcct atggcggtat aacggacatc
      721 attatcggga tgccccacag agggaggctg aatctgttga caggccttct gcagttacct
      781 ccagagctga tgttccgtaa aatgcgaggc ttaagtgagt ttccagaaaa cgttgctgcc
      841 attggtgacg tcctgtccca tctgacctcc tcagtggacc tagactttgg ggcccatcga
      901 cccctccatg tgacaatgct acccaacccc tcacacctgg aagctattaa ccccgtggct
      961 gtagggaaaa ctcgtggcag gcagcagtct caggaagacg gggactactc accaaatggt
     1021 tctgctcagc ctggggacaa agtcatatgc ctgcaggttc atggcgacgc ttccttctgc
     1081 ggtcaaggga ttgttctgga gacgttcacg ctctctaatc tcccacattt cagaatcggt
     1141 gggagcatcc atctcatagt caataaccag ttgggttaca ccacgcctgc tgagagagga
     1201 aggtcgtcct tatacagcag tgacattgga aagctagtag gctgtgccat catccatgtc
     1261 aacggagaca gtccagagga agtggtccga gcgacacgac ttgcttttga gtatcagcgc
     1321 cagttccgca aggacgtgat tattgatttg ttatgctata ggcagtgggg tcacaatgag
     1381 ctggacgaac ctttcttcac caacccggtc atgtacaaaa tcatcagagc tcgaaagagc
     1441 attcctgata cctacgcaga gcacctcatc gccagcggac tcatgacgca ggaggaggtc
     1501 tccgacataa aagcctccta ctacgccaag ttaaatggcc acttggccaa tgtggcccac
     1561 tacagccccc cggccccgca cctgcaggcc cgctggcagg gcctggtgca gccagcagcc
     1621 tgcgtcacca cctgggacac gggcgtgccg ctggagctgc tacggttcgt tggcgtgaag
     1681 tccgtggagg ttccagagga gctgcagtta cacagccacc tgctaaagat gtatgttcag
     1741 tcaagaatgg agaaggtgaa gaatggaacc aatcttgact gggccaccgc agaaacactt
     1801 gccttgggct ccttacttgc tcaaggtttc aatgttcgtc tgagtggcca agatgttggc
     1861 cgtggaactt tcagtcaaag gcatgcaatg gtggtttgcc agaacactga tgatgtctac
     1921 attcctctga accacatgga ccctaaccag aagggctttc tggaggttag caacagcccg
     1981 ctgtcagaag aggctgtctt gggatttgaa tacgggatga gcatcgaaag cccaaagttg
     2041 ctgcccctct gggaagctca gtttggcgat ttcttcaatg gagcccagat catctttgac
     2101 acattcatct ctggaggcga ggccaagtgg ctcctgcaga gtggccttgt catcctcctt
     2161 ccacacggct atgatggagc cgggcctgac cactcatcct gtcggataga gcgcttcttg
     2221 cagatgtgcg acagtgcgga agaaggcgtg gacagcgaca ctgtgaacat gtttgtggtt
     2281 cacccgacta cgcctgctca gtacttccac ttgcttagaa gacagatgat gcggaacttc
     2341 agaaagcctc tcatcgttgc atctcccaaa atgttactca ggtaccctgt agctgtgtca
     2401 acgcttgagg agatggcgcc aggaacagca ttcaaacctg tcattggtga ttcgtcggta
     2461 gaccccaaaa atgtcaaaac cctcatattc tgctccggca aacattttta tgccctgctg
     2521 aagcaaagag agtctctggg ggctaagaag cgtgattttg ccatcattcg actagaggaa
     2581 ctctgccctt tcccgctgga ttcattacaa caagagatgg gcaaatacaa acacgttcaa
     2641 gatatcatct ggagtcagga ggagcctcag aacatgggtc cgtggtcttt tgtttatcca
     2701 aggtttgaaa agcaacttgc ttgcaagctc cgtctcgtaa gtcgaccccc tttgccagcg
     2761 cccgcggtgg gaattggcac agtgcaccag cagcagcatg aagccatcct cttcaagacc
     2821 ttcacttcct aacgtgctct ggggaaacag gatcgccttt taagcaacat gtcccaggag
     2881 aaagcttgtt cctcaactcc ctctcctttc ctttagctta gcgcggaagt ctgtgttcct
     2941 ctgaaattct ggactgattt ggacaactga atctggagtg tgtgggatac acaggggagt
     3001 tgatttttta taagcacatg aaacagacag tagatttcac acttttcagg accacagtgt
     3061 ttgtcatgct ttagttatat gtgagtgaag tggcagagga ggtcatgagg cagttggatt
     3121 ctgactgtac tggaactgac cttaaatgag tagagaacat tcttggccag aacgtggcac
     3181 tgtgtagagg cagccccaac actacctctt aattggttta ttacatgtaa ctttccatat
     3241 tttccctccc ttttaaagtc agcagtattt tcgttttaaa tgtcatctta gagccgctct
     3301 acaaaatgtt ccaacagaca ccactaaaac tagaccattc cgaattcatg ttctttcgag
     3361 ttttatccat tatccttaaa agtaagtgct gttacaccca cattcctgat tgtatgatct
     3421 tctagctctg taccaataca gggtttcctg tagtccagac tggcctcata ttcactgcat
     3481 agctgaagat gaccttgaac ttctgatctt tttacctcta cctcctgagt gctggaattg
     3541 cagacttgtg caattatgcc cagttaatgc tggcaatgag tatccattaa ggatggtagc
     3601 ccccaagctc tctaccaact gagctacagc cctggcccct aataatttta tgttatgaag
     3661 tattagctgt attaaaatgg acttgttctt ttcctattaa gatccccaac ctgttatatt
     3721 gctgtgatat tttctaattt ttcaattaac cggcatttga tagagggatg tcagtgccag
     3781 gttaatcact tttgtctcta aaagttgcag gtacaattat gatgtatatt tttgctataa
     3841 attacattaa gtttagccac cttctcaaac caggcacttg gggttccaca gaggttgaac
     3901 ctagttcgaa gcaacctaag cggtagttat gtggcgtcac ttgaggtggt tctgaggagc
     3961 atctaccatg tacattctgg cttatgatgg attttctaaa aggtgattga ggacatagga
     4021 agtgctgaaa tatatccatt gcctttccgg ttttgccagc catgggccat gtcttacatc
     4081 cttcagtcct ttcttgtgtc aatccttacg aatcttgtaa tatagtacca tttaaattgg
     4141 taaagtttca aaggcaagtt tagtaggaat gtacatttat tctcttatct tttggtttcc
     4201 caaatgaaaa gatattgaaa ctctaactat acaataaatg gaatttgacg tatttaaaaa
     4261 aaaaaaaaaa aaaaaaaaaa
//
