LOCUS       NM_130400                760 bp    mRNA    linear   ROD 03-JUN-2024
DEFINITION  Rattus norvegicus dihydrofolate reductase (Dhfr), mRNA.
ACCESSION   NM_130400
VERSION     NM_130400.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 760)
  AUTHORS   Hughes,L., Carton,R., Minguzzi,S., McEntee,G., Deinum,E.E.,
            O'Connell,M.J. and Parle-McDermott,A.
  TITLE     An active second dihydrofolate reductase enzyme is not a feature of
            rat and mouse, but they do have activity in their mitochondria
  JOURNAL   FEBS Lett 589 (15), 1855-1862 (2015)
   PUBMED   25980602
  REMARK    GeneRIF: demonstrate that dihydrofolate reductase activity is also
            a feature of the mitochondria in both rat and mouse but this is not
            due to a second enzyme
REFERENCE   2  (bases 1 to 760)
  AUTHORS   Whitsett,J., Rangel Filho,A., Sethumadhavan,S., Celinska,J.,
            Widlansky,M. and Vasquez-Vivar,J.
  TITLE     Human endothelial dihydrofolate reductase low activity limits
            vascular tetrahydrobiopterin recycling
  JOURNAL   Free Radic Biol Med 63, 143-150 (2013)
   PUBMED   23707606
REFERENCE   3  (bases 1 to 760)
  AUTHORS   Watkins,D. and Rosenblatt,D.S.
  TITLE     Update and new concepts in vitamin responsive disorders of folate
            transport and metabolism
  JOURNAL   J Inherit Metab Dis 35 (4), 665-670 (2012)
   PUBMED   22108709
  REMARK    Review article
REFERENCE   4  (bases 1 to 760)
  AUTHORS   Gomez-Gomez,Y., Organista-Nava,J., Saavedra-Herrera,M.V.,
            Rivera-Ramirez,A.B., Teran-Porcayo,M.A., Del Carmen
            Alarcon-Romero,L., Illades-Aguiar,B. and Leyva-Vazquez,M.A.
  TITLE     Survival and risk of relapse of acute lymphoblastic leukemia in a
            Mexican population is affected by dihydrofolate reductase gene
            polymorphisms
  JOURNAL   Exp Ther Med 3 (4), 665-672 (2012)
   PUBMED   22969948
REFERENCE   5  (bases 1 to 760)
  AUTHORS   Stover,P.J. and Field,M.S.
  TITLE     Trafficking of intracellular folates
  JOURNAL   Adv Nutr 2 (4), 325-331 (2011)
   PUBMED   22332074
  REMARK    Review article
REFERENCE   6  (bases 1 to 760)
  AUTHORS   Lucke-Huhle,C.
  TITLE     Permissivity for methotrexate-induced DHFR gene amplification
            correlates with the metastatic potential of rat adenocarcinoma
            cells
  JOURNAL   Carcinogenesis 15 (4), 695-700 (1994)
   PUBMED   8149482
REFERENCE   7  (bases 1 to 760)
  AUTHORS   Chu,E., Takimoto,C.H., Voeller,D., Grem,J.L. and Allegra,C.J.
  TITLE     Specific binding of human dihydrofolate reductase protein to
            dihydrofolate reductase messenger RNA in vitro
  JOURNAL   Biochemistry 32 (18), 4756-4760 (1993)
   PUBMED   8490020
REFERENCE   8  (bases 1 to 760)
  AUTHORS   Appleman,J.R., Beard,W.A., Delcamp,T.J., Prendergast,N.J.,
            Freisheim,J.H. and Blakley,R.L.
  TITLE     Unusual transient- and steady-state kinetic behavior is predicted
            by the kinetic scheme operational for recombinant human
            dihydrofolate reductase
  JOURNAL   J Biol Chem 265 (5), 2740-2748 (1990)
   PUBMED   2303423
REFERENCE   9  (bases 1 to 760)
  AUTHORS   Webber,S. and Whiteley,J.M.
  TITLE     Comparative activity of rat liver dihydrofolate reductase with
            7,8-dihydrofolate and other 7,8-dihydropteridines
  JOURNAL   Arch Biochem Biophys 236 (2), 681-690 (1985)
   PUBMED   3970530
REFERENCE   10 (bases 1 to 760)
  AUTHORS   Gewirtz,D.A., White,J.C., Randolph,J.K. and Goldman,I.D.
  TITLE     Transport, binding, and polyglutamation of methotrexate in freshly
            isolated rat hepatocytes
  JOURNAL   Cancer Res 40 (3), 573-578 (1980)
   PUBMED   6162551
COMMENT     PROVISIONAL REFSEQ: This record has not yet been subject to final
            NCBI review. The reference sequence was derived from AF318150.1.
            
            On Aug 31, 2012 this sequence version replaced NM_130400.1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AF318150.1, BC158583.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMD00132261, SAMD00132263
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-760               AF318150.1         2-761
FEATURES             Location/Qualifiers
     source          1..760
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="Sprague-Dawley"
                     /db_xref="taxon:10116"
                     /chromosome="2"
                     /map="2q12"
     gene            1..760
                     /gene="Dhfr"
                     /gene_synonym="Dhfr1"
                     /note="dihydrofolate reductase"
                     /db_xref="GeneID:24312"
                     /db_xref="RGD:2500"
     exon            1..172
                     /gene="Dhfr"
                     /gene_synonym="Dhfr1"
                     /inference="alignment:Splign:2.1.0"
     CDS             87..650
                     /gene="Dhfr"
                     /gene_synonym="Dhfr1"
                     /EC_number="1.5.1.3"
                     /note="Dihydrofolate reductase 1 (active)"
                     /codon_start=1
                     /product="dihydrofolate reductase"
                     /protein_id="NP_569084.1"
                     /db_xref="GeneID:24312"
                     /db_xref="RGD:2500"
                     /translation="MVRPLNCIVAVSQNMGIGKNGDLPWPLLRNEFKYFQRMTTTSSV
                     EGKQNLVIMGRKTWFSIPEKNRPLKDRINIVLSRELKEPPQGAHFLAKSLDDALKLIE
                     QPELASKVDMVWVVGGSSVYQEAMNQPGHLRLFVTRIMQEFESDTFFPEIDLEKYKLL
                     PEYPGVLSEIQEEKGIKYKFEVYEKKD"
     exon            173..222
                     /gene="Dhfr"
                     /gene_synonym="Dhfr1"
                     /inference="alignment:Splign:2.1.0"
     exon            223..328
                     /gene="Dhfr"
                     /gene_synonym="Dhfr1"
                     /inference="alignment:Splign:2.1.0"
     exon            329..455
                     /gene="Dhfr"
                     /gene_synonym="Dhfr1"
                     /inference="alignment:Splign:2.1.0"
     exon            456..571
                     /gene="Dhfr"
                     /gene_synonym="Dhfr1"
                     /inference="alignment:Splign:2.1.0"
     exon            572..760
                     /gene="Dhfr"
                     /gene_synonym="Dhfr1"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 ccttagtggt actccgctcc aggctgcgat ttcgcgccaa acttgacggc actctaagct
       61 ggtggggtct gatcgccgct gcagacatgg ttcgtccgct gaactgcatc gtcgccgtgt
      121 cccaaaatat gggcattggc aagaacggag acttaccctg gcctctgctc aggaatgagt
      181 tcaagtactt ccagagaatg accacaacct cttcagtaga aggtaaacag aatctggtga
      241 ttatgggtag gaaaacctgg ttctccattc ctgagaagaa tcgaccttta aaggacagaa
      301 ttaatatagt tctcagtaga gaactcaagg aaccaccaca aggagctcat tttcttgcca
      361 aaagtctgga tgatgcctta aaacttattg aacaaccaga gctagcaagt aaagtggaca
      421 tggtctgggt agtcggaggc agctctgtct accaggaagc catgaatcag ccaggccacc
      481 tcagactctt tgtgacaagg atcatgcagg agtttgaaag tgacacattc ttcccagaaa
      541 ttgatttgga gaaatataaa cttctcccag aatacccagg cgtcctctct gagatccagg
      601 aggaaaaagg catcaagtac aagtttgaag tctatgaaaa gaaagactaa catgaagatg
      661 ctcccaagtc ctactgcttc tcctaaaagg atgcatttta taagaccact tctgctggct
      721 tgcaatctat gagtaattat ttctttaggg aggggtggct
//
