LOCUS       NM_001394358            5740 bp    mRNA    linear   ROD 26-FEB-2024
DEFINITION  Rattus norvegicus diacylglycerol kinase epsilon (Dgke), transcript
            variant 1, mRNA.
ACCESSION   NM_001394358 XM_006247070
VERSION     NM_001394358.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 5740)
  AUTHORS   Hozumi Y, Fujiwara H, Kaneko K, Fujii S, Topham MK, Watanabe M and
            Goto K.
  TITLE     Diacylglycerol kinase epsilon localizes to subsurface cisterns of
            cerebellar Purkinje cells
  JOURNAL   Cell Tissue Res 368 (3), 441-458 (2017)
   PUBMED   28191598
  REMARK    GeneRIF: findings suggest that DGKepsilon plays an important role
            in neuronal and brain functions through its distinct neuronal
            expression and subcellular localization and also through
            coordinated arrangement with other molecules involving the
            phosphoinositide signaling pathway
REFERENCE   2  (bases 1 to 5740)
  AUTHORS   Hozumi Y, Matsui H, Sakane F, Watanabe M and Goto K.
  TITLE     Distinct expression and localization of diacylglycerol kinase
            isozymes in rat retina
  JOURNAL   J Histochem Cytochem 61 (6), 462-476 (2013)
   PUBMED   23467923
  REMARK    GeneRIF: The DGKepsilon is evident in the outer limiting membrane.
REFERENCE   3  (bases 1 to 5740)
  AUTHORS   Lemaire M, Fremeaux-Bacchi V, Schaefer F, Choi M, Tang WH, Le
            Quintrec M, Fakhouri F, Taque S, Nobili F, Martinez F, Ji W,
            Overton JD, Mane SM, Nurnberg G, Altmuller J, Thiele H, Morin D,
            Deschenes G, Baudouin V, Llanas B, Collard L, Majid MA, Simkova E,
            Nurnberg P, Rioux-Leclerc N, Moeckel GW, Gubler MC, Hwa J, Loirat C
            and Lifton RP.
  TITLE     Recessive mutations in DGKE cause atypical hemolytic-uremic
            syndrome
  JOURNAL   Nat Genet 45 (5), 531-536 (2013)
   PUBMED   23542698
REFERENCE   4  (bases 1 to 5740)
  AUTHORS   Shulga YV, Topham MK and Epand RM.
  TITLE     Substrate specificity of diacylglycerol kinase-epsilon and the
            phosphatidylinositol cycle
  JOURNAL   FEBS Lett 585 (24), 4025-4028 (2011)
   PUBMED   22108654
REFERENCE   5  (bases 1 to 5740)
  AUTHORS   Ghosh D, Lippert D, Krokhin O, Cortens JP and Wilkins JA.
  TITLE     Defining the membrane proteome of NK cells
  JOURNAL   J Mass Spectrom 45 (1), 1-25 (2010)
   PUBMED   19946888
REFERENCE   6  (bases 1 to 5740)
  AUTHORS   Nakano T, Hozumi Y, Goto K and Wakabayashi I.
  TITLE     Localization of diacylglycerol kinase epsilon on stress fibers in
            vascular smooth muscle cells
  JOURNAL   Cell Tissue Res 337 (1), 167-175 (2009)
   PUBMED   19421779
  REMARK    GeneRIF: DGKepsilon interacts with actin stress fibers and is
            involved in their stability in vascular smooth muscle cells.
REFERENCE   7  (bases 1 to 5740)
  AUTHORS   Niizeki T, Takeishi Y, Kitahara T, Arimoto T, Ishino M, Bilim O,
            Suzuki S, Sasaki T, Nakajima O, Walsh RA, Goto K and Kubota I.
  TITLE     Diacylglycerol kinase-epsilon restores cardiac dysfunction under
            chronic pressure overload: a new specific regulator of Galpha(q)
            signaling cascade
  JOURNAL   Am J Physiol Heart Circ Physiol 295 (1), H245-H255 (2008)
   PUBMED   18487437
  REMARK    GeneRIF: Diacylglycerol kinase-epsilon restores cardiac dysfunction
            under chronic pressure overload: a new specific regulator of
            Galpha(q) signaling cascade.
REFERENCE   8  (bases 1 to 5740)
  AUTHORS   Epand RM, Shulga YV, Timmons HC, Perri AL, Belani JD, Perinpanathan
            K, Johnson-McIntire LB, Bajjalieh S, Dicu AO, Elias C, Rychnovsky
            SD and Topham MK.
  TITLE     Substrate chirality and specificity of diacylglycerol kinases and
            the multisubstrate lipid kinase
  JOURNAL   Biochemistry 46 (49), 14225-14231 (2007)
   PUBMED   18004883
REFERENCE   9  (bases 1 to 5740)
  AUTHORS   Epand RM, Kam A, Bridgelal N, Saiga A and Topham MK.
  TITLE     The alpha isoform of diacylglycerol kinase exhibits arachidonoyl
            specificity with alkylacylglycerol
  JOURNAL   Biochemistry 43 (46), 14778-14783 (2004)
   PUBMED   15544348
REFERENCE   10 (bases 1 to 5740)
  AUTHORS   Rodriguez de Turco EB, Tang W, Topham MK, Sakane F, Marcheselli VL,
            Chen C, Taketomi A, Prescott SM and Bazan NG.
  TITLE     Diacylglycerol kinase epsilon regulates seizure susceptibility and
            long-term potentiation through arachidonoyl- inositol lipid
            signaling
  JOURNAL   Proc Natl Acad Sci U S A 98 (8), 4740-4745 (2001)
   PUBMED   11287665
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            JACYVU010000220.1.
            
            [WARNING] On Apr 3, 2024 this sequence was replaced by
            NM_001394358.2.
            
            On Apr 15, 2021 this sequence version replaced XM_006247070.4.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            RNAseq introns :: mixed sample support SAMD00132261, SAMD00132262
                              [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            inferred exon combination :: based on alignments, homology
            RefSeq Select criteria    :: based on conservation, expression,
                                         longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-807               JACYVU010000220.1  30701332-30702138   c
            808-967             JACYVU010000220.1  30696261-30696420   c
            968-1087            JACYVU010000220.1  30693341-30693460   c
            1088-1231           JACYVU010000220.1  30692000-30692143   c
            1232-1389           JACYVU010000220.1  30691561-30691718   c
            1390-1441           JACYVU010000220.1  30690868-30690919   c
            1442-1555           JACYVU010000220.1  30689676-30689789   c
            1556-1627           JACYVU010000220.1  30685486-30685557   c
            1628-1755           JACYVU010000220.1  30682983-30683110   c
            1756-1867           JACYVU010000220.1  30682272-30682383   c
            1868-5740           JACYVU010000220.1  30677888-30681760   c
FEATURES             Location/Qualifiers
     source          1..5740
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="BN"
                     /db_xref="taxon:10116"
                     /chromosome="10"
                     /map="10q26"
     gene            1..5740
                     /gene="Dgke"
                     /note="diacylglycerol kinase epsilon"
                     /db_xref="GeneID:497978"
                     /db_xref="RGD:1560914"
     exon            1..807
                     /gene="Dgke"
                     /inference="alignment:Splign:2.1.0"
     CDS             350..2053
                     /gene="Dgke"
                     /EC_number="2.7.1.107"
                     /note="isoform 1 is encoded by transcript variant 1;
                     diacylglycerol kinase, epsilon 64kDa"
                     /codon_start=1
                     /product="diacylglycerol kinase epsilon isoform 1"
                     /protein_id="NP_001381287.1"
                     /db_xref="GeneID:497978"
                     /db_xref="RGD:1560914"
                     /translation="MEGDQRSGPSAQGLLPDGHLILWTLFSVLLPVFITLWCSLQRSR
                     RQLHRRDIFRKSKHCWRDTDLFSHPTYCCVCAQHILQGAFCDCCGLRVDEGCLKKVDK
                     RFPCKEIMLKNDSRAADAMPHHWIRGNVPLCTYCVICRQQCGSQPKLCDYRCVWCQQT
                     VHDECMRGSLKSEKCDFGEFKNLIIPPSYLTSINQMRKDKNTNYEVLASKFGKQWTPL
                     IILANSRSGTNMGEGLLGEFKMLLNPVQVFDVTKTPPIKALQLCTLLPYYSVRVLVCG
                     GDGTVGWVLDAIDEMKIKGQEKYIPEVAVLPLGTGNDLSNTLGWGTGYAGEIPVAQVL
                     RNVMEADGIKLDRWKVQVTNKGYYSLRKPKEFTMNNYFSIGPDALMALNFHAHREKAP
                     SLFSSRILNKAVYLFYGTRDCLVQECKDLNKKIELELDGERVELPNLEGVIVLNIGYW
                     GGGCRLWEGVGDETYPLARHDDGLLEVVGVYGSFHCAQIQVKLANPFRIGQAHTVRLT
                     LKCSRMPMPMQVDGEPWAQGPCTVTITHKTHALMLYFSGEQSDDDLSSPSDHEDLKEA
                     E"
     exon            808..967
                     /gene="Dgke"
                     /inference="alignment:Splign:2.1.0"
     exon            968..1087
                     /gene="Dgke"
                     /inference="alignment:Splign:2.1.0"
     exon            1088..1231
                     /gene="Dgke"
                     /inference="alignment:Splign:2.1.0"
     exon            1232..1389
                     /gene="Dgke"
                     /inference="alignment:Splign:2.1.0"
     exon            1390..1441
                     /gene="Dgke"
                     /inference="alignment:Splign:2.1.0"
     exon            1442..1555
                     /gene="Dgke"
                     /inference="alignment:Splign:2.1.0"
     exon            1556..1627
                     /gene="Dgke"
                     /inference="alignment:Splign:2.1.0"
     exon            1628..1755
                     /gene="Dgke"
                     /inference="alignment:Splign:2.1.0"
     exon            1756..1867
                     /gene="Dgke"
                     /inference="alignment:Splign:2.1.0"
     exon            1868..5740
                     /gene="Dgke"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 cgcaggtgcg ctcctcaagc gcggcctcct catcccacgc acatctcaca gcgtgagggt
       61 gggacggcgg ccagaggctt ggctggaggt ggtacccgag accgcgagag cgaactgggg
      121 tctccagaga cctcgcgata ggggcgcggg cgaggcccag cgcgacggcc gttgtgagca
      181 cggtggccac gggccgggcc tgtgctgcac ggagcccgcg ggtgcgacct gcgcctttcc
      241 cggggccgag gccacgcgca gcgaagacga gctgcaaccc cacgctgtga gtcctagagc
      301 aagtcccctg gacccggcct ctccgtaggc cacgcctggg cctgggaaga tggagggaga
      361 ccagcggtcc ggcccatcgg cgcagggcct gctccccgat ggccacttga tcctatggac
      421 gctgttctcc gtgctgttgc cggtgttcat caccttatgg tgtagtctgc agcgttcgcg
      481 ccggcagctg caccgcaggg acatcttccg caagagcaag cactgctggc gtgacaccga
      541 cctgttcagc caccccacct actgctgcgt gtgcgcccag cacatcctgc aaggagcctt
      601 ctgcgactgc tgcgggctcc gcgtggacga gggctgcctc aagaaggtcg acaagcgctt
      661 cccgtgcaag gagatcatgc tcaagaacga cagcagggcc gcagatgcga tgccccacca
      721 ctggatccgc ggcaacgtcc ccctgtgcac ttactgtgta atctgcaggc agcagtgtgg
      781 cagccagcct aaactctgcg actaccggtg tgtttggtgc cagcagacag tccacgatga
      841 gtgcatgaga ggcagcttaa agagtgagaa gtgtgatttt ggagaattca aaaatctcat
      901 catccctcca agttatttaa cgtccattaa ccagatgcgt aaagacaaaa acacaaatta
      961 tgaagtgctt gcttctaagt ttggaaagca gtggacccca ttaataatcc tggccaactc
     1021 tcgtagtgga actaacatgg gagaaggact gttgggagag tttaagatgc tgttaaatcc
     1081 cgtccaggtg ttcgatgtca ctaaaactcc tccgatcaaa gctctgcagc tgtgcactct
     1141 tcttccttat tactccgttc gagtccttgt ttgtggaggg gatgggactg tgggctgggt
     1201 cctcgatgca attgatgaaa tgaagattaa gggacaagaa aaatacattc cagaggttgc
     1261 agtcttacct ctgggaacag gcaacgacct atccaacacc ctgggctggg gtactggcta
     1321 tgctggagaa atcccggttg cacaggtctt aaggaatgta atggaagcag atggaatcaa
     1381 actagacaga tggaaagtgc aggtaacaaa taaaggctac tacagtttaa ggaaacccaa
     1441 ggagttcaca atgaacaact acttctctat tggacccgat gccctcatgg ctctcaattt
     1501 tcatgctcat cgtgagaagg cgccgtctct gttttccagc cgaatcctta acaaggctgt
     1561 ttatttattc tatggaacta gagattgctt ggtgcaagag tgtaaagatt tgaataaaaa
     1621 aattgagcta gagctggatg gtgagcgagt tgaactgcct aacttggagg gagttattgt
     1681 tctgaacatt ggctactggg gcggtggctg ccgactgtgg gaaggagtgg gagatgagac
     1741 gtatcctcta gccagacatg atgatggctt actggaagtt gttggagtat atgggtcttt
     1801 ccactgtgct caaatccagg tgaaactggc aaatcctttt cgaatcggac aggcccatac
     1861 cgtgcggctg actttgaagt gctccaggat gccgatgccg atgcaggtgg atggggagcc
     1921 ctgggcccaa gggccctgta ctgtcaccat aacgcacaag acacatgcac tgatgctgta
     1981 cttctctggg gagcagtcgg atgatgacct ctcaagccct tcagatcacg aggacttaaa
     2041 ggaggccgag tagaaggctg agatgaagcg tatctgccca gatgggtgac atgtcgccca
     2101 caggacactg ctggccgtca gctgtcattt ccttcctagc attgtgtgtg agcatgtgaa
     2161 cagcatttcc acagctagac ttcagtatca cagacacact ctcctgtcag caacacactc
     2221 tgatcattat cactaactga aaagaaaaac ccaccacaga gaggtccagg aagatgaaac
     2281 acttctcccg gctggacatt gtaagactgg cctcacgccg cattgtgcac tgccttggtg
     2341 gacaggatgt ggacggagag atcattccct ccctcagttc ttggtgtgta ttaacgcgtt
     2401 aaacgttagc ttgcttttga actgccctgg ttaacatggg cttcctcccg cactgtgcca
     2461 aggaagatgc cccacacctc aaaccaaatg gaccctgcat tctgattcct aggagcacct
     2521 gaggagaaaa gtgaaagaaa gatggcgctt accattttca cagtagatat ttcctatcag
     2581 gctttcagat tttcacaacc atattgaact gaagtgcaaa atcttttttt ttaattgttt
     2641 ttgtttggga agtactaagg attgaacctc tggaagtcta gatgaatgct ctatcattga
     2701 actacactcc agcactggtg tgtatgcaca cacaaacaca cacacacaca cacacacaca
     2761 cacacacaca cacgtatata tgtatataca actagatagg tagatggggc taaagtaaga
     2821 tttagaaaac tttggaatgg aagctctaca gttcaccagc ttttctttag actaaaagat
     2881 atcattgact ggaaaaaata gacacataag ggaagcctcc aagtcagatt agttactcaa
     2941 aaaatagtcc aggctgactg ccttcaagtt gagtggtctt gtgtagtgaa acactgcagg
     3001 actcttgaca gcattgccct cagagaggga gctctgctaa gatgtttgta tcctatttat
     3061 ttcaaagtat gaaagatata tttttaactt attgtttacc tgtggcgtga tgcttctgtt
     3121 aagcgtttac actgtattgt atagctttat ttagtaaagt ggtcacataa atctgctggt
     3181 cacagtcaca tagcactctc tctctctgtt ccatggcata tatactcact gtgctcattc
     3241 ctcagacctt aaaagggagg gcacatgaat aagaaagaac ttgaggggtt ggggatttag
     3301 ctcagtggta gagcacttgc ctaggaagcg caaggccctg ggttcggtcc ccagctccgg
     3361 gaaaaaaaaa aaaaattaga aaaaaaagaa agaacttgag gtcccgcaaa gcttcctttc
     3421 tttccttgcc cctccggcct ccattgggaa gcacttattg aaggcaggcc catcgcttcc
     3481 attagggggc gctgtgccgc tgctgtccga ggcctgcctc tgcagttcgc cacaccagtg
     3541 ggcatctggc ttggtggtct ttcgtgaatt aatttctgaa agactagaat ctactcccaa
     3601 accaccattt gtctcccttt aatggtttct tatgaatctg cagtcagtga gttgccataa
     3661 acgtcagttt agtcacctca ggcccagacc atgtttttga aagaacaact tagtaagtaa
     3721 aatattagta cattatccaa agtaatttta ccctgctggg ttaacctttt ccacctgttc
     3781 ctgggggcta tcaaattcta ctgttgaaag atgttccatg ggtttgctcc aacttacacc
     3841 ttcagagtgg atccgcagcc gaggtttgag agccgtgaga gtttgtacct tgttccttgt
     3901 gagctgatcg tgtttggtgt gtaatgagat tcatccttag agtcgtggtc agaattgtta
     3961 gagagtcacc ctctgaggca tactattaaa tttattgtgg atatatttag ttacctgtat
     4021 ttcataatgt tctgtttgtg gtaaaacaat ttattaattt ggggctatca gtcaagatag
     4081 ggtaactacc gagattctcc aggtagagga ggcttgtgtc cccttgccag gacacgccat
     4141 gatgtctttc tgatgcctta ctcagattcc atatgtaact tccacaatac tgtatcgtaa
     4201 aacagaccag tattttattt actgcagctc acatttaggc actagtttac ccagcattcc
     4261 agacaaatgt ataggcatgt gccttgtttg aatacaattt gtaatacttg gtgtttgcaa
     4321 gttatatatt agatggtgat agcttattac aagcctctgt acttcacaaa atgattgact
     4381 atgagatccc tctgttccat ttaaagttgt ctccatagca acacaactta cacattctca
     4441 ggaaaaacta agaaccatct ctttgtatct gtttccacat ctcttgtctt ttctgctcta
     4501 atacattcct cacgcctcat gaacttagtg gcttaaactc cgaataggtc cttagggata
     4561 gttttcagcg ctttgcatat gtaatagtgt cagaggcttt cctgtctcag agtaggcatt
     4621 gtccagtagt gaggtaactg aacttaaatg ttttagactt tctgcaactt agctgtgaac
     4681 ctgtaatgta gctgatattt ataaaaaaat tttatagtga aaactattta ttttattttt
     4741 ttagagtgtg gaaggaaaat ctgtagcaat tactttttga gtatttgtgt gcctggttgt
     4801 aatgaggctg acttagaaaa gttctttttg tgctgtgtaa atttagtatt tctgccagca
     4861 tgtcagaaag taattttcac ctataacata aagtaaaata ttcctagtct acactgtggg
     4921 attttaattt gcacttgcta ggaaacttga aaggttatga tgatttgcac atcaatatgg
     4981 acctaaatac ataatctgac cagaaaggtc agtggaatag gcaatgacat catgatactt
     5041 ttgttcaaaa tgaaactatg gaagcaagaa accagcactt tatattcaga aatggggggg
     5101 gggggtgtta atttggtttc tgtgactttt tttaaatttc tgaatggttt aggacaaggg
     5161 atggatttta agtctagaaa ttttaaagga aaaatagttt tgttgcccag cctcatgacg
     5221 tcacatgctc agcagcagct ctcctttaag agccaggagg gccacaagtg tggttgaggg
     5281 gtgaactcct gccccttttt ggatcaactt ggcaatccca atacctagag gctaagttca
     5341 aggccagcct ggctgtacgt gaggccctta tctcaaagca gttgcttgca ttctaacccg
     5401 attggtgacc tagctattgg aaagtgatag ctcctggagt gacctagctt tcagataagt
     5461 tggtggagcg attagtcttt agattagtgc gtggactctt ggaggacggg gagtaggtgc
     5521 tggtggagca cctcagtcag tgtggcatta ctctgcactg agccttactg agtcatatac
     5581 aagaccagta gataaaatat ttccactatt taaaaaaaaa caaccctcag tagggcatgg
     5641 tagcaatatc tgttatctca gagagaggca ggaagatctc tgagttcaag gccggccagc
     5701 caaggctacc taaaccctgt ctcaaaaaaa aaaaaaaaaa
//
