LOCUS       NM_019304               6608 bp    mRNA    linear   ROD 03-APR-2024
DEFINITION  Rattus norvegicus diacylglycerol kinase, beta (Dgkb), mRNA.
ACCESSION   NM_019304 XM_346768
VERSION     NM_019304.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 6608)
  AUTHORS   Sekerkova,G., Watanabe,M., Martina,M. and Mugnaini,E.
  TITLE     Differential distribution of phospholipase C beta isoforms and
            diaglycerol kinase-beta in rodents cerebella corroborates the
            division of unipolar brush cells into two major subtypes
  JOURNAL   Brain Struct Funct 219 (2), 719-749 (2014)
   PUBMED   23503970
  REMARK    GeneRIF: This study demonistrated that dgkb distribuate in rat
            cerebellum.
REFERENCE   2  (bases 1 to 6608)
  AUTHORS   Hozumi,Y., Matsui,H., Sakane,F., Watanabe,M. and Goto,K.
  TITLE     Distinct expression and localization of diacylglycerol kinase
            isozymes in rat retina
  JOURNAL   J Histochem Cytochem 61 (6), 462-476 (2013)
   PUBMED   23467923
  REMARK    GeneRIF: The DGKbeta localize in the outer plexiform layer, within
            which photoreceptor cells make contact with bipolar and horizontal
            cells.
REFERENCE   3  (bases 1 to 6608)
  AUTHORS   Sato,M., Liu,K., Sasaki,S., Kunii,N., Sakai,H., Mizuno,H., Saga,H.
            and Sakane,F.
  TITLE     Evaluations of the selectivities of the diacylglycerol kinase
            inhibitors R59022 and R59949 among diacylglycerol kinase isozymes
            using a new non-radioactive assay method
  JOURNAL   Pharmacology 92 (1-2), 99-107 (2013)
   PUBMED   23949095
REFERENCE   4  (bases 1 to 6608)
  AUTHORS   Archambaud,C., Nahori,M.A., Soubigou,G., Becavin,C., Laval,L.,
            Lechat,P., Smokvina,T., Langella,P., Lecuit,M. and Cossart,P.
  TITLE     Impact of lactobacilli on orally acquired listeriosis
  JOURNAL   Proc Natl Acad Sci U S A 109 (41), 16684-16689 (2012)
   PUBMED   23012479
REFERENCE   5  (bases 1 to 6608)
  AUTHORS   Gellett,A.M., Kharel,Y., Sunkara,M., Morris,A.J. and Lynch,K.R.
  TITLE     Biosynthesis of alkyl lysophosphatidic acid by diacylglycerol
            kinases
  JOURNAL   Biochem Biophys Res Commun 422 (4), 758-763 (2012)
   PUBMED   22627129
REFERENCE   6  (bases 1 to 6608)
  AUTHORS   Yahagi,H., Takeda,M., Asaumi,Y., Okumura,K., Takahashi,R.,
            Takahashi,J., Ohta,J., Tada,H., Minatoya,Y., Sakuma,M.,
            Watanabe,J., Goto,K., Shirato,K. and Kagaya,Y.
  TITLE     Differential regulation of diacylglycerol kinase isozymes in
            cardiac hypertrophy
  JOURNAL   Biochem Biophys Res Commun 332 (1), 101-108 (2005)
   PUBMED   15896305
  REMARK    GeneRIF: These results suggest that DGKepsilon and DGKzeta play
            distinct roles in the development of pressure overloaded cardiac
            hypertrophy and that the two isozymes are differentially regulated.
REFERENCE   7  (bases 1 to 6608)
  AUTHORS   Kwitek,A.E., Gullings-Handley,J., Yu,J., Carlos,D.C., Orlebeke,K.,
            Nie,J., Eckert,J., Lemke,A., Andrae,J.W., Bromberg,S., Pasko,D.,
            Chen,D., Scheetz,T.E., Casavant,T.L., Soares,M.B., Sheffield,V.C.,
            Tonellato,P.J. and Jacob,H.J.
  TITLE     High-density rat radiation hybrid maps containing over 24,000
            SSLPs, genes, and ESTs provide a direct link to the rat genome
            sequence
  JOURNAL   Genome Res 14 (4), 750-757 (2004)
   PUBMED   15060019
REFERENCE   8  (bases 1 to 6608)
  AUTHORS   Cipres,A., Carrasco,S., Merino,E., Diaz,E., Krishna,U.M.,
            Falck,J.R., Martinez-A,C. and Merida,I.
  TITLE     Regulation of diacylglycerol kinase alpha by phosphoinositide
            3-kinase lipid products
  JOURNAL   J Biol Chem 278 (37), 35629-35635 (2003)
   PUBMED   12832407
REFERENCE   9  (bases 1 to 6608)
  AUTHORS   Caricasole,A., Bettini,E., Sala,C., Roncarati,R., Kobayashi,N.,
            Caldara,F., Goto,K. and Terstappen,G.C.
  TITLE     Molecular cloning and characterization of the human diacylglycerol
            kinase beta (DGKbeta) gene: alternative splicing generates DGKbeta
            isotypes with different properties
  JOURNAL   J Biol Chem 277 (7), 4790-4796 (2002)
   PUBMED   11719522
REFERENCE   10 (bases 1 to 6608)
  AUTHORS   Goto,K. and Kondo,H.
  TITLE     Molecular cloning and expression of a 90-kDa diacylglycerol kinase
            that predominantly localizes in neurons
  JOURNAL   Proc Natl Acad Sci U S A 90 (16), 7598-7602 (1993)
   PUBMED   7689223
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            JAXUCZ010000006.1.
            
            On Dec 5, 2020 this sequence version replaced NM_019304.1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: D16100.1, SRR26360194.160636.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA5760393, SAMEA5760433
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-333               JAXUCZ010000006.1  60368588-60368920
            334-589             JAXUCZ010000006.1  60479046-60479301
            590-666             JAXUCZ010000006.1  60554966-60555042
            667-687             JAXUCZ010000006.1  60558197-60558217
            688-841             JAXUCZ010000006.1  60571353-60571506
            842-982             JAXUCZ010000006.1  60589835-60589975
            983-1032            JAXUCZ010000006.1  60595383-60595432
            1033-1107           JAXUCZ010000006.1  60598697-60598771
            1108-1227           JAXUCZ010000006.1  60601736-60601855
            1228-1345           JAXUCZ010000006.1  60609848-60609965
            1346-1434           JAXUCZ010000006.1  60612427-60612515
            1435-1551           JAXUCZ010000006.1  60612595-60612711
            1552-1650           JAXUCZ010000006.1  60620707-60620805
            1651-1683           JAXUCZ010000006.1  60642797-60642829
            1684-1800           JAXUCZ010000006.1  60655431-60655547
            1801-1874           JAXUCZ010000006.1  60666402-60666475
            1875-1949           JAXUCZ010000006.1  60672679-60672753
            1950-2035           JAXUCZ010000006.1  60689201-60689286
            2036-2125           JAXUCZ010000006.1  60690940-60691029
            2126-2286           JAXUCZ010000006.1  60703190-60703350
            2287-2351           JAXUCZ010000006.1  60811889-60811953
            2352-2442           JAXUCZ010000006.1  60949707-60949797
            2443-2638           JAXUCZ010000006.1  60956952-60957147
            2639-2759           JAXUCZ010000006.1  61086219-61086339
            2760-2820           JAXUCZ010000006.1  61087582-61087642
            2821-6608           JAXUCZ010000006.1  61120633-61124420
FEATURES             Location/Qualifiers
     source          1..6608
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="BN"
                     /db_xref="taxon:10116"
                     /chromosome="6"
                     /map="6q21"
     gene            1..6608
                     /gene="Dgkb"
                     /gene_synonym="Dagk; DGK-beta"
                     /note="diacylglycerol kinase, beta"
                     /db_xref="GeneID:54248"
                     /db_xref="RGD:2488"
     exon            1..333
                     /gene="Dgkb"
                     /gene_synonym="Dagk; DGK-beta"
                     /inference="alignment:Splign:2.1.0"
     exon            334..589
                     /gene="Dgkb"
                     /gene_synonym="Dagk; DGK-beta"
                     /inference="alignment:Splign:2.1.0"
     CDS             520..2928
                     /gene="Dgkb"
                     /gene_synonym="Dagk; DGK-beta"
                     /EC_number="2.7.1.107"
                     /note="diacylglycerol kinase beta, 90kDa; DAG kinase beta;
                     diglyceride kinase beta; 90 kDa diacylglycerol kinase;
                     Diacylglycerol kinase 90kDa"
                     /codon_start=1
                     /product="diacylglycerol kinase beta"
                     /protein_id="NP_062177.2"
                     /db_xref="GeneID:54248"
                     /db_xref="RGD:2488"
                     /translation="MTNQEKWAHLSPSEFSQLQKYAEYSTKKLKDVLEEFHGNGVLAK
                     YNPEGKQDILNQTIDFEGFKLFMKTFLEAELPDDFTAHLFMSFSNKFPHSSPNVKSKP
                     ALLSGGLRMNKGAITPPRSSPANTCSPEVIHLKDIVCYLSLLERGRPEDKLEFMFRLY
                     DTDGNGFLDSSELENIIGQMMHVAEYLEWDVTELNPILHEMMEEIDYDRDGTVSLEEW
                     IQGGMTTIPLLVLLGLENNVKDDGQHVWRLKHFNKPAYCNLCLNMLIGVGKQGLCCSF
                     CKYTVHERCVARAPPSCIKTYVKSKKNTDVMHHYWVEGNCPTKCDKCHKTVKCYQGLT
                     GLHCVWCQTTLHNKCASHLKPECDCGPLKDHILPPTTICPVVLTMPTAGTSVPEERQS
                     TAKKEKGSSQQPNKVTDKNKMQRANSVTMDGQGLQITPIPGTHPLLVFVNPKSGGKQG
                     ERIYRKFQYLLNPRQVYSLSGNGPMPGLHFFRDVPDFRVLACGGDGTVGWILDCIEKA
                     NVVKHPPVAILPLGTGNDLARCLRWGGGYEGENLMKILKDIESSTEIMLDRWKFEVTP
                     NDKDEKGDPVPYSIINNYFSIGVDASIAHRFHIMREKHPEKFNSRMKNKFWYFEFGTS
                     ETFSATCKKLHESVEIECDGVQIDLINISLEGIAILNIPSMHGGSNLWGESKKKRSHR
                     RIEKKGSDKRPTLTDAKELKFASQDLSDQLLEVVGLEGAMEMGQIYTGLKSAGRRLAQ
                     CSSVVIRTSKSLPMQIDGEPWMQTPCTIKITHKNQAPMLMGPPPKTGLFCSLIKRTRN
                     RSKE"
     exon            590..666
                     /gene="Dgkb"
                     /gene_synonym="Dagk; DGK-beta"
                     /inference="alignment:Splign:2.1.0"
     exon            667..687
                     /gene="Dgkb"
                     /gene_synonym="Dagk; DGK-beta"
                     /inference="alignment:Splign:2.1.0"
     exon            688..841
                     /gene="Dgkb"
                     /gene_synonym="Dagk; DGK-beta"
                     /inference="alignment:Splign:2.1.0"
     exon            842..982
                     /gene="Dgkb"
                     /gene_synonym="Dagk; DGK-beta"
                     /inference="alignment:Splign:2.1.0"
     exon            983..1032
                     /gene="Dgkb"
                     /gene_synonym="Dagk; DGK-beta"
                     /inference="alignment:Splign:2.1.0"
     exon            1033..1107
                     /gene="Dgkb"
                     /gene_synonym="Dagk; DGK-beta"
                     /inference="alignment:Splign:2.1.0"
     exon            1108..1227
                     /gene="Dgkb"
                     /gene_synonym="Dagk; DGK-beta"
                     /inference="alignment:Splign:2.1.0"
     exon            1228..1345
                     /gene="Dgkb"
                     /gene_synonym="Dagk; DGK-beta"
                     /inference="alignment:Splign:2.1.0"
     exon            1346..1434
                     /gene="Dgkb"
                     /gene_synonym="Dagk; DGK-beta"
                     /inference="alignment:Splign:2.1.0"
     exon            1435..1551
                     /gene="Dgkb"
                     /gene_synonym="Dagk; DGK-beta"
                     /inference="alignment:Splign:2.1.0"
     exon            1552..1650
                     /gene="Dgkb"
                     /gene_synonym="Dagk; DGK-beta"
                     /inference="alignment:Splign:2.1.0"
     exon            1651..1683
                     /gene="Dgkb"
                     /gene_synonym="Dagk; DGK-beta"
                     /inference="alignment:Splign:2.1.0"
     exon            1684..1800
                     /gene="Dgkb"
                     /gene_synonym="Dagk; DGK-beta"
                     /inference="alignment:Splign:2.1.0"
     exon            1801..1874
                     /gene="Dgkb"
                     /gene_synonym="Dagk; DGK-beta"
                     /inference="alignment:Splign:2.1.0"
     exon            1875..1949
                     /gene="Dgkb"
                     /gene_synonym="Dagk; DGK-beta"
                     /inference="alignment:Splign:2.1.0"
     exon            1950..2035
                     /gene="Dgkb"
                     /gene_synonym="Dagk; DGK-beta"
                     /inference="alignment:Splign:2.1.0"
     exon            2036..2125
                     /gene="Dgkb"
                     /gene_synonym="Dagk; DGK-beta"
                     /inference="alignment:Splign:2.1.0"
     exon            2126..2286
                     /gene="Dgkb"
                     /gene_synonym="Dagk; DGK-beta"
                     /inference="alignment:Splign:2.1.0"
     exon            2287..2351
                     /gene="Dgkb"
                     /gene_synonym="Dagk; DGK-beta"
                     /inference="alignment:Splign:2.1.0"
     exon            2352..2442
                     /gene="Dgkb"
                     /gene_synonym="Dagk; DGK-beta"
                     /inference="alignment:Splign:2.1.0"
     exon            2443..2638
                     /gene="Dgkb"
                     /gene_synonym="Dagk; DGK-beta"
                     /inference="alignment:Splign:2.1.0"
     exon            2639..2759
                     /gene="Dgkb"
                     /gene_synonym="Dagk; DGK-beta"
                     /inference="alignment:Splign:2.1.0"
     exon            2760..2820
                     /gene="Dgkb"
                     /gene_synonym="Dagk; DGK-beta"
                     /inference="alignment:Splign:2.1.0"
     exon            2821..6608
                     /gene="Dgkb"
                     /gene_synonym="Dagk; DGK-beta"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 agcctccgct gtttttgagt tgcaaatgat ctgggagtgg tgggttgcag gcagcccgtg
       61 ccgtcgcctg gaagaagact tccatctttc agtcactagt tccaacgggc cgtgcgttct
      121 ggaagggggt ggcagcaggg aggaggatgc tctgagcaga gttcttgaag ctttgctctt
      181 gggggaagcc gggctgtgtg ggagccttct gactgagagg agagcgtgtg aattggaaag
      241 gatcctgaga ggcggctggt cccagttcct ctccggaaaa gctgtcgctc tcgcttcaga
      301 gatgcgccta gccttcgcct gcatcacgct gcatgcttta acaaattatt gaaattacaa
      361 acatcaacag cagtttcatt tcgacagagt ccatattctg gaagtccagg ctattttatc
      421 atgaaacaca ccttgtaaaa tctttgaagc attgaattct gaccaagatc tggataaaga
      481 agccctcatc tgctgtgtaa gaatctcatc acctacgcca tgacaaacca ggaaaaatgg
      541 gcccatctca gcccctcaga attttcccaa cttcagaaat atgctgagta ttctacaaag
      601 aaattaaagg atgttcttga agaattccat ggtaatggtg tgcttgcaaa gtataatcct
      661 gaagggaaac aagatattct taaccaaacc atagattttg aaggattcaa gctgttcatg
      721 aagaccttcc tggaagctga gctccctgac gatttcactg cccacctttt catgtcattc
      781 agcaacaaat tcccccattc cagccccaac gtaaagagca agccagctct gctttcaggt
      841 ggtttgagga tgaataaggg tgccatcacc cctccacgtt cttctccagc aaatacgtgc
      901 tccccggaag tgatccacct caaggatatt gtctgttacc tgtctcttct ggaaagagga
      961 agacctgagg acaagcttga gtttatgttt cgcctgtatg acaccgatgg gaatggcttc
     1021 ctggacagct cggaattaga aaatatcatc ggtcagatga tgcatgttgc cgagtacctg
     1081 gaatgggatg ttactgaact taatccgatc cttcatgaaa tgatggaaga aatcgactat
     1141 gaccgtgacg gcactgtgtc tctggaagag tggattcaag gaggaatgac gactatccca
     1201 cttctagtct tgctgggctt ggaaaacaat gtgaaggatg acggacagca tgtgtggcga
     1261 ctcaagcact ttaacaagcc cgcctactgc aatctctgcc tgaacatgct gattggcgtg
     1321 gggaagcagg gcctctgctg ctccttctgc aagtacaccg tccatgagcg ctgcgtggcc
     1381 agagccccgc cctcctgcat caagacctat gtgaagtcca agaagaacac tgacgtcatg
     1441 caccattatt gggtcgaagg caattgccca accaagtgtg ataagtgcca caagacagtg
     1501 aaatgttacc agggcctgac ggggctgcac tgtgtttggt gtcagaccac gctgcacaat
     1561 aaatgtgctt ctcatctgaa gcctgaatgt gactgtggac ccttgaagga ccatattttg
     1621 ccacccacca caatctgtcc agttgtattg actatgccca ctgccggaac ttcagttcct
     1681 gaggaaagac agtcaacagc taaaaaggaa aagggcagct cccagcagcc aaataaagtg
     1741 acagacaaga acaaaatgca aagagctaac tctgtcacta tggatggaca aggtttgcag
     1801 atcactccta ttcctggcac acatcctctt ctagtttttg tcaaccctaa aagtggtggg
     1861 aagcaaggag aacgaattta cagaaaattc cagtatcttc taaatcctcg tcaggtttac
     1921 agtctttctg gaaatggacc aatgccaggg ttacattttt ttcgcgatgt ccctgacttc
     1981 agagtgttag catgcggtgg agatgggact gtgggctgga ttttggactg catagaaaag
     2041 gccaatgtag tcaagcaccc tccagttgct atcctgcctc ttgggacggg caatgaccta
     2101 gcaagatgcc tgcggtgggg tggaggttat gaaggtgaaa atcttatgaa gatcctaaaa
     2161 gacattgaaa gcagcacaga aatcatgttg gacaggtgga agtttgaagt cacacccaac
     2221 gacaaagacg agaaaggaga ccctgtgcct tacagcatca tcaacaacta cttttccatt
     2281 ggcgtggatg cttccattgc acacagattc cacatcatga gagaaaagca cccggagaaa
     2341 ttcaacagcc gaatgaagaa caaattttgg tactttgagt ttggcacatc agaaaccttc
     2401 tcagccacct gcaagaaact gcatgaatct gtagaaatag aatgtgatgg agtacagata
     2461 gatttgataa acatctctct ggaaggaatt gcaattttga atataccaag catgcatgga
     2521 ggatctaatc tttggggaga atctaaaaag aaaagaagtc atcgacggat agagaaaaaa
     2581 ggctctgaca aaagacccac tctcacagat gcaaaagaac tgaagtttgc aagtcaagat
     2641 ctgagtgacc agctactgga ggttgttggc ttggaaggag caatggaaat gggccaaata
     2701 tacacggggc tgaaaagtgc tggccggcgg ctggctcagt gctcctctgt ggttatcagg
     2761 acaagcaagt cactgccaat gcagattgat ggagagccat ggatgcagac cccatgcaca
     2821 ataaaaatta cacacaagaa ccaggcccca atgctgatgg gacctccacc taaaactgga
     2881 ttattctgct ccctcatcaa aagaacaaga aaccgaagca aggaatgaac ctccgtcagg
     2941 agaaatttga atagcatact tgtaccacgg aacattttga cccatttcaa gtctcctgct
     3001 cctacaaaat catggaattc acagtttttg ttattgagct gatgtaaaat ttggctatta
     3061 gaaaatttgt tgtctctgtt ttcataggcg tatttgcatg gaaaaaaagt agaaatttat
     3121 atgatgcgac acagcgtatt taagttgcat gcactcacac agggtttctg tgtctcctcc
     3181 tcacatcttc tgccattttc atttactgac tgtgaggaaa aacctgcgaa agatcaaaga
     3241 agtatgatgg gaaaagattt cttatataat tgctgtcatt ggcaagcagt aataactatg
     3301 gctaaaacaa cctcaagtgc aaactgtcat cctttgccta ttagaaaaat cactgcatcc
     3361 ttgatgttgg acccatgaca taggaagacg ctaaattcaa aaattttatt aaaaagttgc
     3421 ccatgatacc atgctccatc taaatactct tttttaaatc tctttactct tcccagtgga
     3481 catgagaata tcaggcataa gattttttct tgtgctaaaa cactagaaag tgacttttat
     3541 tttgtttagc tttgttttta actccaaaaa cacgagagca aatatgttac taggaggaat
     3601 tgtagtaaca tagcaagctt tattcctttc tgcataagaa tctagacaca aatgcataat
     3661 cacagtagta gccaaatgga cggtttaaac tatgtgccaa acgaatctgt ttcccaccaa
     3721 agatcaggaa ttagagttct tacgagtttg ggaaaagtaa aatgtgagac aagaatgagt
     3781 tacccattca ttgtgataaa aatgttcacc attcaaaacc agcgaatcat acagtataat
     3841 caaatctcaa ttgccaattt catggctatt tggtgccccc accatatatg tctgtgattt
     3901 ttaatggatc tgttaagtgc acttcagaag gtaaatgaca gcgttggttc ctttttgggt
     3961 agattggtac ctgcgacaat actgagctca ttgaagcaac catgcatgca attagcctct
     4021 tactagttat tcatccctgc cccatcttta tgacatttca aatgtttggg gatatataaa
     4081 ggcctcctta aggtgtgtgc atagcatgat ctggtctctg attatttaaa gaaatgtgca
     4141 agtgctgctt ttcatagtgt ttctttgagg ctatcctagg aaacgaaata cttcggggca
     4201 gtctcagtgt ggttcagtta cactgacctg catctcatca ccgcacgatt tatgttttca
     4261 gacgcaacgc catcaggaga atgagtgctc gaaatgcatg atttatcagc tttcctctct
     4321 gctctacatt aaagtgctaa tgatttatca gttctatttt tctattgatt ctttcatctt
     4381 tgaataacaa atagcagtta gtgattctgt tgaacacaac cccgtgcatg gatttattga
     4441 tttccagtga aattgctgta attttacttc tttcgttgga aaactcaagt gaatgctgtt
     4501 gtgtcaaata gctgctctca agcagtgttc tgtaatggaa gattatgtat aaagtgtatt
     4561 cctctcgtcg tcctggaaca ttggatgcta aatacccatc aacaataact ttaattgctt
     4621 taaaaataaa agcttttgaa tagaattaag acataattta gcttacccag ggaaatgatg
     4681 attaaaattg caaattcagt acagcaatca gttgtcctag tatagtaata ataaagtagt
     4741 tattcatcag aatagtaatc tagaccaaaa gaatgctcga aacaactaca aacgcatcac
     4801 tcacataatg ttacataaca catcctttgc ttttgtgcat ctctggttac aggtaataaa
     4861 tatttcttac acgaattgat gagatttttc tacagtgggc aggtagacct tgagccaaaa
     4921 tgttattttg aatccacaat aatttcacgt tctggaggac attaaagcag cagtagtttc
     4981 tgaattattt gcagcatctg ggaaactgta gtagaagact aaagaactgg cctgaatggt
     5041 ttcagtcact gaatagtggg cccctgctgc actttagcat ggctgcatga ccacaaccag
     5101 ttctagtagg cactacattt cagtccattg gattcatgga taaccctagt aagtcttcaa
     5161 aagggaatca attttgactt tcctaaattc gatttttttt aaaaagataa ctgatgtgct
     5221 tgccctttga gtacactact cctggggttt ggaaagtagt tttttaaaat ctactttgat
     5281 ggttttgaaa taagagaaga tattctcttc ttttttatga cagcttcgtg tgaagaatat
     5341 atattttaag gaaaattcta aatggaaagt tctatggcat attaaaatac tgaataatga
     5401 ttgttagctt aacaaaatgg aaactttact tccaggcatg tgggtgtcgg aggtgttgag
     5461 tcaacctttt ttaactatca tagacataat aagtgataaa gttctgagtg aagtggtgtg
     5521 aaaatgaatg ctgcactagc ttaaaatggt cagagaatct caaggataga gtttcatacc
     5581 ctgctaaaca ctgccatata ctcagttcat agacagaaat gacagattct agaataaacc
     5641 tggaaatgta cactttcagg aaataggaag ggcgatatcg acgtgcagtc agcatttcaa
     5701 acgtcacgtg tgtattttct cactctccgt catgtgtatt tgatgtggag agtggcgatc
     5761 gagtttttgt aatagaagaa aagatgcttt actttgaatg ggttatgtgg ttgctgcctt
     5821 tctagagctc tctgatcacg ggttcattgt acgcagtctc ctcaaggcaa gaatgtctaa
     5881 tggtaagcgc ggtggagtca cacatccatt cccctttgta cacgtgactt gtattagaga
     5941 gtggggctaa gtacctttac ctggaccgaa tctgtttaag tctgagactg tcgatgccac
     6001 ttacactagc tagggctctt cggtggttcc tttccttttt ctgttagtgt tcgctggttt
     6061 ttgattggtt ttgaaaggaa cggtgtattt ttatggtaca tataaccctc gaagagttaa
     6121 tcgatggata acttatattc cacattaaag gatatacctt tctagcactt atagcatata
     6181 ttgagactgt gggatgtaat aaaaataata aatgaattta agaaaagcct tttgttctgt
     6241 gaaagacaga aatcacatcc ttaaaccagc caattgaatt gaattatgtt taaaggtttg
     6301 agtaaattct catttgaacc tttctatata ttcttattgt gtttaaaaca tagggtttgt
     6361 ttaattaaac tatcttactt attttctcag aaagtgttat gtacaaatcc tggtttttga
     6421 aataaaagtg ttaaccattc acttgtgcat catttgttct cttccagatg gtcccttcat
     6481 gtgaaatatc cattagaaca tcctcagtgg ttttccacta gaggacctgt gcaagccgcc
     6541 ttgtcttagg aataaaaaca ctgtgcttag gggttaaatt acttgtcatt aaaaaccaag
     6601 ccattcaa
//
