LOCUS       NM_080787               3043 bp    mRNA    linear   ROD 03-APR-2024
DEFINITION  Rattus norvegicus diacylglycerol kinase, alpha (Dgka), mRNA.
ACCESSION   NM_080787
VERSION     NM_080787.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 3043)
  AUTHORS   Cui,H., Huang,Y., Wu,Y., Ma,J., Cao,X., Xie,J. and Zhang,Y.
  TITLE     The expression of diacylglycerol kinase isoforms alpha and zeta
            correlates with the progression of experimental autoimmune
            encephalomyelitis in rats
  JOURNAL   Histochem Cell Biol 156 (5), 437-448 (2021)
   PUBMED   34312706
  REMARK    GeneRIF: The expression of diacylglycerol kinase isoforms alpha and
            zeta correlates with the progression of experimental autoimmune
            encephalomyelitis in rats.
REFERENCE   2  (bases 1 to 3043)
  AUTHORS   Hozumi,Y., Matsui,H., Sakane,F., Watanabe,M. and Goto,K.
  TITLE     Distinct expression and localization of diacylglycerol kinase
            isozymes in rat retina
  JOURNAL   J Histochem Cytochem 61 (6), 462-476 (2013)
   PUBMED   23467923
  REMARK    GeneRIF: The DGKalpha localize predominantly to the nucleus of
            ganglion cells.
REFERENCE   3  (bases 1 to 3043)
  AUTHORS   Sato,M., Liu,K., Sasaki,S., Kunii,N., Sakai,H., Mizuno,H., Saga,H.
            and Sakane,F.
  TITLE     Evaluations of the selectivities of the diacylglycerol kinase
            inhibitors R59022 and R59949 among diacylglycerol kinase isozymes
            using a new non-radioactive assay method
  JOURNAL   Pharmacology 92 (1-2), 99-107 (2013)
   PUBMED   23949095
REFERENCE   4  (bases 1 to 3043)
  AUTHORS   Gellett,A.M., Kharel,Y., Sunkara,M., Morris,A.J. and Lynch,K.R.
  TITLE     Biosynthesis of alkyl lysophosphatidic acid by diacylglycerol
            kinases
  JOURNAL   Biochem Biophys Res Commun 422 (4), 758-763 (2012)
   PUBMED   22627129
REFERENCE   5  (bases 1 to 3043)
  AUTHORS   Ghosh,D., Lippert,D., Krokhin,O., Cortens,J.P. and Wilkins,J.A.
  TITLE     Defining the membrane proteome of NK cells
  JOURNAL   J Mass Spectrom 45 (1), 1-25 (2010)
   PUBMED   19946888
REFERENCE   6  (bases 1 to 3043)
  AUTHORS   Epand,R.M., Shulga,Y.V., Timmons,H.C., Perri,A.L., Belani,J.D.,
            Perinpanathan,K., Johnson-McIntire,L.B., Bajjalieh,S., Dicu,A.O.,
            Elias,C., Rychnovsky,S.D. and Topham,M.K.
  TITLE     Substrate chirality and specificity of diacylglycerol kinases and
            the multisubstrate lipid kinase
  JOURNAL   Biochemistry 46 (49), 14225-14231 (2007)
   PUBMED   18004883
REFERENCE   7  (bases 1 to 3043)
  AUTHORS   Epand,R.M., Kam,A., Bridgelal,N., Saiga,A. and Topham,M.K.
  TITLE     The alpha isoform of diacylglycerol kinase exhibits arachidonoyl
            specificity with alkylacylglycerol
  JOURNAL   Biochemistry 43 (46), 14778-14783 (2004)
   PUBMED   15544348
REFERENCE   8  (bases 1 to 3043)
  AUTHORS   Cipres,A., Carrasco,S., Merino,E., Diaz,E., Krishna,U.M.,
            Falck,J.R., Martinez-A,C. and Merida,I.
  TITLE     Regulation of diacylglycerol kinase alpha by phosphoinositide
            3-kinase lipid products
  JOURNAL   J Biol Chem 278 (37), 35629-35635 (2003)
   PUBMED   12832407
REFERENCE   9  (bases 1 to 3043)
  AUTHORS   Goto,K., Watanabe,M., Kondo,H., Yuasa,H., Sakane,F. and Kanoh,H.
  TITLE     Gene cloning, sequence, expression and in situ localization of 80
            kDa diacylglycerol kinase specific to oligodendrocyte of rat brain
  JOURNAL   Brain Res Mol Brain Res 16 (1-2), 75-87 (1992)
   PUBMED   1339302
REFERENCE   10 (bases 1 to 3043)
  AUTHORS   Schaap,D., de Widt,J., van der Wal,J., Vandekerckhove,J., van
            Damme,J., Gussow,D., Ploegh,H.L., van Blitterswijk,W.J. and van der
            Bend,R.L.
  TITLE     Purification, cDNA-cloning and expression of human diacylglycerol
            kinase
  JOURNAL   FEBS Lett 275 (1-2), 151-158 (1990)
   PUBMED   2175712
COMMENT     PROVISIONAL REFSEQ: This record has not yet been subject to final
            NCBI review. The reference sequence was derived from S49760.1.
            
            ##Evidence-Data-START##
            Transcript exon combination :: S49760.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMD00132261,
                                           SAMD00132262 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
FEATURES             Location/Qualifiers
     source          1..3043
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /db_xref="taxon:10116"
                     /chromosome="7"
                     /map="7q11"
     gene            1..3043
                     /gene="Dgka"
                     /gene_synonym="Dagk1"
                     /note="diacylglycerol kinase, alpha"
                     /db_xref="GeneID:140866"
                     /db_xref="RGD:70904"
     exon            1..451
                     /gene="Dgka"
                     /gene_synonym="Dagk1"
                     /inference="alignment:Splign:2.1.0"
     exon            452..593
                     /gene="Dgka"
                     /gene_synonym="Dagk1"
                     /inference="alignment:Splign:2.1.0"
     CDS             530..2713
                     /gene="Dgka"
                     /gene_synonym="Dagk1"
                     /EC_number="2.7.1.107"
                     /EC_number="2.7.1.93"
                     /note="DGK-alpha; DAG kinase alpha; diglyceride kinase
                     alpha; 80 kDa diacylglycerol kinase"
                     /codon_start=1
                     /product="diacylglycerol kinase alpha"
                     /protein_id="NP_542965.1"
                     /db_xref="GeneID:140866"
                     /db_xref="RGD:70904"
                     /translation="MAKDKGLISPEDFAQLQKYIDYSTKSVSDVLKVFEMNKYCQGDE
                     IGYLGFEQFLKMYLEVEEVPHHLCWTLFWSFHSSQDLDEETESKANVICLSDVYCYFT
                     LLEGGSPEDKLEFTFKLYDMDRNGILDSTEVEKIILQMMRVAEYLDWDVSELRPILQE
                     MMKEMDRDGSGCVSLAEWVRAGATTVPLLVLLGIDMTMKDDGHHIWRPKRFSRPVYCN
                     LCELSIGLGKQGLSCNLCKYIVHDHCAMKAQPCEVSTYAKSRKDIGVQPHVWVRGGCH
                     SGRCDRCQKKIRTYHSLTGLHCVWCHLEIHDDCLQAVGPECDCGLLRDHILPPCSIYP
                     RVLVSGQECKQKTTDVTSLCTPEAFRIEPVSNTHPLLVFINPKSGGKQGQSVLWKFQY
                     ILNPRQVFNLKDGPEPGLRFFKDVPQFRVLVCGGDGTVGWILETIDKANFPIVPPVAV
                     LPLGTGNDLARCLRWGRGYEGENLRKILKDIEISKVVYLDRWLLEVIPQQNGEKSDPV
                     PSQIINNYFSIGVDASIAHRFHLMREKYPEKFNSRMKNKLWYLEFATSESIFSTCKKL
                     EESVTVEICGKLLDLSDLSLEGIAVLNIPSMHGGSNLWGDTKRPHGDTCGINQALGSV
                     AKIITDPDILKTCVPDMSDKRLEVVGIEGVIEMGQIYTRLKSAGHRLAKCSEITFQTT
                     KTLPMQVDGEPWMQAPCTIKITHKNQMPMLMGPAPSSSNFFGFWS"
     exon            594..658
                     /gene="Dgka"
                     /gene_synonym="Dagk1"
                     /inference="alignment:Splign:2.1.0"
     exon            659..794
                     /gene="Dgka"
                     /gene_synonym="Dagk1"
                     /inference="alignment:Splign:2.1.0"
     exon            795..872
                     /gene="Dgka"
                     /gene_synonym="Dagk1"
                     /inference="alignment:Splign:2.1.0"
     exon            873..922
                     /gene="Dgka"
                     /gene_synonym="Dagk1"
                     /inference="alignment:Splign:2.1.0"
     exon            923..997
                     /gene="Dgka"
                     /gene_synonym="Dagk1"
                     /inference="alignment:Splign:2.1.0"
     exon            998..1117
                     /gene="Dgka"
                     /gene_synonym="Dagk1"
                     /inference="alignment:Splign:2.1.0"
     exon            1118..1232
                     /gene="Dgka"
                     /gene_synonym="Dagk1"
                     /inference="alignment:Splign:2.1.0"
     exon            1233..1321
                     /gene="Dgka"
                     /gene_synonym="Dagk1"
                     /inference="alignment:Splign:2.1.0"
     exon            1322..1441
                     /gene="Dgka"
                     /gene_synonym="Dagk1"
                     /inference="alignment:Splign:2.1.0"
     exon            1442..1540
                     /gene="Dgka"
                     /gene_synonym="Dagk1"
                     /inference="alignment:Splign:2.1.0"
     exon            1541..1609
                     /gene="Dgka"
                     /gene_synonym="Dagk1"
                     /inference="alignment:Splign:2.1.0"
     exon            1610..1683
                     /gene="Dgka"
                     /gene_synonym="Dagk1"
                     /inference="alignment:Splign:2.1.0"
     exon            1684..1755
                     /gene="Dgka"
                     /gene_synonym="Dagk1"
                     /inference="alignment:Splign:2.1.0"
     exon            1756..1841
                     /gene="Dgka"
                     /gene_synonym="Dagk1"
                     /inference="alignment:Splign:2.1.0"
     exon            1842..1931
                     /gene="Dgka"
                     /gene_synonym="Dagk1"
                     /inference="alignment:Splign:2.1.0"
     exon            1932..2092
                     /gene="Dgka"
                     /gene_synonym="Dagk1"
                     /inference="alignment:Splign:2.1.0"
     exon            2093..2157
                     /gene="Dgka"
                     /gene_synonym="Dagk1"
                     /inference="alignment:Splign:2.1.0"
     exon            2158..2248
                     /gene="Dgka"
                     /gene_synonym="Dagk1"
                     /inference="alignment:Splign:2.1.0"
     exon            2249..2447
                     /gene="Dgka"
                     /gene_synonym="Dagk1"
                     /inference="alignment:Splign:2.1.0"
     exon            2448..2568
                     /gene="Dgka"
                     /gene_synonym="Dagk1"
                     /inference="alignment:Splign:2.1.0"
     exon            2569..2629
                     /gene="Dgka"
                     /gene_synonym="Dagk1"
                     /inference="alignment:Splign:2.1.0"
     exon            2630..3037
                     /gene="Dgka"
                     /gene_synonym="Dagk1"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 ggaagacaga agcgtgggag tggagaaagt ggcacaaacc agagccctgg agatactctg
       61 gaaacgcgga gcagattgtg ttggaaagct ggcaacagcg gtggtctcgg gcgcggcggg
      121 agggcaggct gggtcggggg cgggcagtgt gtcgtcagga gctgggcggc cccgcggcgg
      181 ccgcgtcttc ctagcccatc ccctcaagcc cagctcgggc tccggcgccc cagctccgac
      241 cgtgcgctct cctgggtcaa gttagagccc accgagagcc ccgcgccact cgcgcactct
      301 cgcacccggg cagaaggact gcggaagggg ccgccccacg ggctgggtga gggctgcagc
      361 gtccagagaa ggaagcgttg acagctggag ccggctgctg gaagggtttg cgccgggacg
      421 cgggggttgc cgtagcgcta cgcaagacgg ggcttaccct ctgaaaagca aacataagag
      481 gctgtacaac caggttatct ctgcgatcag cctcaagcag agaagaaaga tggccaaaga
      541 caagggcctc atcagcccag aagactttgc ccagctgcaa aagtacatag actactccac
      601 caaaagtgtc agcgatgtgc tgaaggtctt tgagatgaac aaatattgcc aaggagatga
      661 gattgggtac ctgggatttg aacagttcct gaaaatgtat ctggaagtgg aggaggttcc
      721 ccatcaccta tgctggactc tgttttggtc cttccatagc agtcaagact tggatgagga
      781 gactgagtca aaagccaatg tgatctgtct cagtgacgtc tactgctact tcaccctcct
      841 ggaaggcggc agtccggaag acaagctaga gttcaccttc aagctgtacg acatggacag
      901 aaatgggatc ctagacagca cagaagtaga aaaaatcatc ctgcaaatga tgagagtggc
      961 tgaatatctg gactgggatg tgtctgagct gagaccgatc cttcaggaga tgatgaaaga
     1021 gatggaccgg gatggcagtg gctgtgtctc cctagctgag tgggtccggg ctggggctac
     1081 cactgtgccc ctgcttgtcc ttctggggat agacatgact atgaaagatg atggacacca
     1141 tatatggaga cccaagagat tctccagacc ggtctactgc aacctgtgcg agttgagcat
     1201 tggcctcggc aaacaaggcc tgagctgtaa cctctgtaag tacattgttc atgaccactg
     1261 tgccatgaag gcccagcctt gtgaagtcag cacctatgcc aagtctcgga aagacattgg
     1321 tgtccagcca cacgtatggg ttcgaggagg ctgtcattct ggacgctgcg accgctgtca
     1381 gaaaaagatc cggacctacc acagcctaac gggactgcac tgcgtgtggt gccacctgga
     1441 gatccacgat gactgtctgc aggctgttgg tcccgagtgt gactgtggac tgctccgtga
     1501 tcatatcctg cctccgtgtt ctatctaccc cagggtcctg gtatctggac aggagtgcaa
     1561 acagaagacc acagatgtta cgagcctgtg cacccctgag gcttttcgga ttgaacctgt
     1621 ttctaacacc cacccccttc tggtcttcat caatcctaag agcggaggca agcaggggca
     1681 gagcgtgctt tggaagttcc agtacattct gaaccctcgg caggtgttta acctgaagga
     1741 tggtccggag ccagggctca ggtttttcaa agacgttcct cagttccggg tgttggtgtg
     1801 tggtggagac ggcaccgtag gctggattct agagaccatt gacaaagcca actttcccat
     1861 tgtgcctcca gtcgctgtgt tgcccctggg cactggaaat gacctggctc ggtgcctaag
     1921 atggggaaga ggttatgaag gtgagaactt gagaaagatt ctcaaggata tagagataag
     1981 taaggtggta tatctcgatc gatggctcct ggaagtgata ccccaacaaa acggagaaaa
     2041 gagtgatcca gttccctctc aaatcatcaa taactacttc tccattggtg tggatgcttc
     2101 cattgctcac cggttccatc tcatgagaga gaaataccct gagaagttca atagcagaat
     2161 gaagaacaag ctttggtact tggagtttgc cacatcggag tccatcttct caacgtgcaa
     2221 aaagctggaa gagtcagtga ccgtcgagat atgtgggaag ctgctggatc tgagtgacct
     2281 gtccctcgaa ggcattgcgg tattgaatat cccgagcatg catggtggct ccaatctctg
     2341 gggtgacacc aagagacctc acggggatac gtgtgggatc aaccaggcac tgggcagtgt
     2401 ggccaaaata atcacagacc ccgatattct caaaacctgt gtgccagaca tgagtgacaa
     2461 gcggctggaa gtcgtaggaa tagagggtgt gattgagatg ggtcagatct ataccaggct
     2521 caagagtgct ggacaccggc tggccaagtg ctccgagatc acgttccaga ccacaaaaac
     2581 cctccctatg caagttgatg gagaaccctg gatgcaagca ccctgtacaa tcaagatcac
     2641 ccataagaac cagatgccta tgctaatggg tccggctccc agttcctcca atttctttgg
     2701 cttttggagc tgaggatgga tgccatctgc cttgagccca cctccctgtt cctggagatt
     2761 tcccactatc tagatgctgc cacaccttcc tgccagccca gaaggatgtt ccatcacctt
     2821 cacagtattt attatcctcc gccacctcac tgctcccaca cagacatcct tacacaacca
     2881 gcgatgcgta accttgaaaa tgcctcatct aataaagtga ctttttccat cactgggata
     2941 tctgttaaaa tgagcgacac atctcttttt acaccttcac ccctctacag acagatttaa
     3001 aagcagacag aacaaataaa tgaacaaaga aagcctaaaa aaa
//
