LOCUS       NM_053437               1880 bp    mRNA    linear   ROD 03-APR-2024
DEFINITION  Rattus norvegicus diacylglycerol O-acyltransferase 1 (Dgat1), mRNA.
ACCESSION   NM_053437 XM_346804
VERSION     NM_053437.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 1880)
  AUTHORS   Ma,Z., Onorato,J.M., Chen,L., Nelson,D.W., Yen,C.E. and Cheng,D.
  TITLE     Synthesis of neutral ether lipid monoalkyl-diacylglycerol by lipid
            acyltransferases
  JOURNAL   J Lipid Res 58 (6), 1091-1099 (2017)
   PUBMED   28420705
REFERENCE   2  (bases 1 to 1880)
  AUTHORS   Leamy,A.K., Hasenour,C.M., Egnatchik,R.A., Trenary,I.A., Yao,C.H.,
            Patti,G.J., Shiota,M. and Young,J.D.
  TITLE     Knockdown of triglyceride synthesis does not enhance palmitate
            lipotoxicity or prevent oleate-mediated rescue in rat hepatocytes
  JOURNAL   Biochim Biophys Acta 1861 (9 Pt A), 1005-1014 (2016)
   PUBMED   27249207
  REMARK    GeneRIF: In both wild-type and DGAT-knockdown hepatocytes, oleate
            co-treatment significantly reduced PA lipid incorporation and
            overall phospholipid saturation compared to PA-treated hepatocytes
REFERENCE   3  (bases 1 to 1880)
  AUTHORS   Tuohetahuntila,M., Molenaar,M.R., Spee,B., Brouwers,J.F.,
            Houweling,M., Vaandrager,A.B. and Helms,J.B.
  TITLE     ATGL and DGAT1 are involved in the turnover of newly synthesized
            triacylglycerols in hepatic stellate cells
  JOURNAL   J Lipid Res 57 (7), 1162-1174 (2016)
   PUBMED   27179362
  REMARK    GeneRIF: TAG synthesis and levels of PUFA-TAGs were lowered by the
            diacylglycerol acyltransferase (DGAT)1 inhibitor, T863. The lipase
            inhibitor, Atglistatin, increased the levels of TAG in both WT and
            ATGL-deficient mouse Hepatic stellate cell (HSC). Both Atglistatin
            and T863 inhibited the induction of activation marker, alpha-smooth
            muscle actin, in rat HSCs, but not in mouse HSCs.
REFERENCE   4  (bases 1 to 1880)
  AUTHORS   Schober,G., Arnold,M., Birtles,S., Buckett,L.K., Pacheco-Lopez,G.,
            Turnbull,A.V., Langhans,W. and Mansouri,A.
  TITLE     Diacylglycerol acyltransferase-1 inhibition enhances intestinal
            fatty acid oxidation and reduces energy intake in rats
  JOURNAL   J Lipid Res 54 (5), 1369-1384 (2013)
   PUBMED   23449193
  REMARK    GeneRIF: pharmacological DGAT-1 inhibition leads to an increase in
            intestinal FAO and ketogenesis when dietary fat is available.
REFERENCE   5  (bases 1 to 1880)
  AUTHORS   Jin,K., Norris,K. and Vaziri,N.D.
  TITLE     Dysregulation of hepatic fatty acid metabolism in chronic kidney
            disease
  JOURNAL   Nephrol Dial Transplant 28 (2), 313-320 (2013)
   PUBMED   23045433
REFERENCE   6  (bases 1 to 1880)
  AUTHORS   Kelpe,C.L., Johnson,L.M. and Poitout,V.
  TITLE     Increasing triglyceride synthesis inhibits glucose-induced insulin
            secretion in isolated rat islets of langerhans: a study using
            adenoviral expression of diacylglycerol acyltransferase
  JOURNAL   Endocrinology 143 (9), 3326-3332 (2002)
   PUBMED   12193544
REFERENCE   7  (bases 1 to 1880)
  AUTHORS   Yen,C.L., Stone,S.J., Cases,S., Zhou,P. and Farese,R.V. Jr.
  TITLE     Identification of a gene encoding MGAT1, a monoacylglycerol
            acyltransferase
  JOURNAL   Proc Natl Acad Sci U S A 99 (13), 8512-8517 (2002)
   PUBMED   12077311
REFERENCE   8  (bases 1 to 1880)
  AUTHORS   Cheng,D., Meegalla,R.L., He,B., Cromley,D.A., Billheimer,J.T. and
            Young,P.R.
  TITLE     Human acyl-CoA:diacylglycerol acyltransferase is a tetrameric
            protein
  JOURNAL   Biochem J 359 (Pt 3), 707-714 (2001)
   PUBMED   11672446
REFERENCE   9  (bases 1 to 1880)
  AUTHORS   Cases,S., Stone,S.J., Zhou,P., Yen,E., Tow,B., Lardizabal,K.D.,
            Voelker,T. and Farese,R.V. Jr.
  TITLE     Cloning of DGAT2, a second mammalian diacylglycerol
            acyltransferase, and related family members
  JOURNAL   J Biol Chem 276 (42), 38870-38876 (2001)
   PUBMED   11481335
REFERENCE   10 (bases 1 to 1880)
  AUTHORS   Smith,S.J., Cases,S., Jensen,D.R., Chen,H.C., Sande,E., Tow,B.,
            Sanan,D.A., Raber,J., Eckel,R.H. and Farese,R.V. Jr.
  TITLE     Obesity resistance and multiple mechanisms of triglyceride
            synthesis in mice lacking Dgat
  JOURNAL   Nat Genet 25 (1), 87-90 (2000)
   PUBMED   10802663
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            JAXUCZ010000007.1.
            
            On Dec 5, 2020 this sequence version replaced NM_053437.1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AB062762.1, AB062761.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA5760383,
                                           SAMEA5760386 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-359               JAXUCZ010000007.1  110114658-110115016 c
            360-447             JAXUCZ010000007.1  110109160-110109247 c
            448-488             JAXUCZ010000007.1  110107186-110107226 c
            489-574             JAXUCZ010000007.1  110107008-110107093 c
            575-627             JAXUCZ010000007.1  110106871-110106923 c
            628-733             JAXUCZ010000007.1  110106641-110106746 c
            734-841             JAXUCZ010000007.1  110106394-110106501 c
            842-916             JAXUCZ010000007.1  110106235-110106309 c
            917-1020            JAXUCZ010000007.1  110106041-110106144 c
            1021-1059           JAXUCZ010000007.1  110105919-110105957 c
            1060-1101           JAXUCZ010000007.1  110105802-110105843 c
            1102-1146           JAXUCZ010000007.1  110105682-110105726 c
            1147-1259           JAXUCZ010000007.1  110105485-110105597 c
            1260-1325           JAXUCZ010000007.1  110105342-110105407 c
            1326-1413           JAXUCZ010000007.1  110105167-110105254 c
            1414-1476           JAXUCZ010000007.1  110105020-110105082 c
            1477-1880           JAXUCZ010000007.1  110104514-110104917 c
FEATURES             Location/Qualifiers
     source          1..1880
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="BN"
                     /db_xref="taxon:10116"
                     /chromosome="7"
                     /map="7q34"
     gene            1..1880
                     /gene="Dgat1"
                     /gene_synonym="ARAT; Dgat"
                     /note="diacylglycerol O-acyltransferase 1"
                     /db_xref="GeneID:84497"
                     /db_xref="RGD:628673"
     exon            1..359
                     /gene="Dgat1"
                     /gene_synonym="ARAT; Dgat"
                     /inference="alignment:Splign:2.1.0"
     CDS             127..1629
                     /gene="Dgat1"
                     /gene_synonym="ARAT; Dgat"
                     /EC_number="2.3.1.20"
                     /EC_number="2.3.1.76"
                     /note="diglyceride acyltransferase; diacylglycerol
                     acyltransferase; acyl-coenzyme A:diacylglycerol
                     acyltransferase 1; retinol O-fatty-acyltransferase;
                     acyl-CoA retinol O-fatty-acyltransferase"
                     /codon_start=1
                     /product="diacylglycerol O-acyltransferase 1"
                     /protein_id="NP_445889.2"
                     /db_xref="GeneID:84497"
                     /db_xref="RGD:628673"
                     /translation="MGDRGGAGSSRRRRTGSRVSIQGGSGPMVDEEEVRDAAVGPDLG
                     AGGDAPAPAPVPAPAHTRDKDRQTSVGDGHWELRCHRLQDSLFSSDSGFSNYRGILNW
                     CVVMLILSNARLFLENLIKYGILVDPIQVVSLFLKDPYSWPAPCLIIASNIFIVATFQ
                     IEKRLSVGALTEQMGLLLHVVNLATIICFPAAVALLVESITPVGSLFALASYSIIFLK
                     LFSYRDVNLWCRQRRVKAKAVSAGKKVSGAAAQNTVSYPDNLTYRDLYYFIFAPTLCY
                     ELNFPRSPRIRKRFLLRRVLEMLFFTQLQVGLIQQWMVPTIQNSMKPFKDMDYSRIIE
                     RLLKLAVPNHLIWLIFFYWLFHSCLNAVAELLQFGDREFYRDWWNAESVTYFWQNWNI
                     PVHKWCIRHFYKPMLRLGSNKWMARTGVFLASAFFHEYLVSIPLRMFRLWAFTAMMAQ
                     VPLAWIVNRFFQGNYGNAAVWVTLIIGQPVAVLMYVHDYYVLNYDAPVGA"
     exon            360..447
                     /gene="Dgat1"
                     /gene_synonym="ARAT; Dgat"
                     /inference="alignment:Splign:2.1.0"
     exon            448..488
                     /gene="Dgat1"
                     /gene_synonym="ARAT; Dgat"
                     /inference="alignment:Splign:2.1.0"
     exon            489..574
                     /gene="Dgat1"
                     /gene_synonym="ARAT; Dgat"
                     /inference="alignment:Splign:2.1.0"
     exon            575..627
                     /gene="Dgat1"
                     /gene_synonym="ARAT; Dgat"
                     /inference="alignment:Splign:2.1.0"
     exon            628..733
                     /gene="Dgat1"
                     /gene_synonym="ARAT; Dgat"
                     /inference="alignment:Splign:2.1.0"
     exon            734..841
                     /gene="Dgat1"
                     /gene_synonym="ARAT; Dgat"
                     /inference="alignment:Splign:2.1.0"
     exon            842..916
                     /gene="Dgat1"
                     /gene_synonym="ARAT; Dgat"
                     /inference="alignment:Splign:2.1.0"
     exon            917..1020
                     /gene="Dgat1"
                     /gene_synonym="ARAT; Dgat"
                     /inference="alignment:Splign:2.1.0"
     exon            1021..1059
                     /gene="Dgat1"
                     /gene_synonym="ARAT; Dgat"
                     /inference="alignment:Splign:2.1.0"
     exon            1060..1101
                     /gene="Dgat1"
                     /gene_synonym="ARAT; Dgat"
                     /inference="alignment:Splign:2.1.0"
     exon            1102..1146
                     /gene="Dgat1"
                     /gene_synonym="ARAT; Dgat"
                     /inference="alignment:Splign:2.1.0"
     exon            1147..1259
                     /gene="Dgat1"
                     /gene_synonym="ARAT; Dgat"
                     /inference="alignment:Splign:2.1.0"
     exon            1260..1325
                     /gene="Dgat1"
                     /gene_synonym="ARAT; Dgat"
                     /inference="alignment:Splign:2.1.0"
     exon            1326..1413
                     /gene="Dgat1"
                     /gene_synonym="ARAT; Dgat"
                     /inference="alignment:Splign:2.1.0"
     exon            1414..1476
                     /gene="Dgat1"
                     /gene_synonym="ARAT; Dgat"
                     /inference="alignment:Splign:2.1.0"
     exon            1477..1880
                     /gene="Dgat1"
                     /gene_synonym="ARAT; Dgat"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 ggcagggggc ggcggcggag gcggaggccg aggccgttgc tcagggtcca gaggcggggc
       61 cgcgggctcc aggcgctgtg agccggcgga gcacgactgg gccgcgacgt ggtgcgggcc
      121 gaagccatgg gcgaccgcgg aggcgcggga agctctcggc gtcggaggac cggctcgcgg
      181 gtttccatcc agggtggtag tgggcccatg gtagacgaag aggaggtgcg agacgccgct
      241 gtgggccccg acttgggcgc cgggggtgac gctccggctc cggctccggt tccggctcca
      301 gcccacaccc gggacaaaga ccggcagacc agcgtgggcg acggccactg ggagctgagg
      361 tgccatcgtc tgcaagactc tttgttcagc tcagacagcg gtttcagcaa ttaccgtggt
      421 atcctgaatt ggtgcgtggt gatgctgatc ctgagtaatg caaggttatt tttagagaat
      481 cttatcaagt atggcatcct ggtggatccc atccaggtgg tgtctctgtt tctgaaggac
      541 ccctacagct ggcctgcccc atgcttgatc attgcatcca atatctttat tgtggctaca
      601 tttcagattg agaagcgcct gtcagtgggt gccctgacag agcagatggg gctgctgcta
      661 catgtggtta acctggccac aattatctgc ttcccagcag ctgtggcctt actggttgag
      721 tctatcactc cagtgggttc cctgtttgct ctggcatcat actccatcat cttcctcaag
      781 cttttctcct accgggatgt caatctgtgg tgccgccagc gaagggtcaa ggccaaagct
      841 gtgtctgcag ggaagaaggt cagtggggct gctgcccaga acactgtaag ctatccggac
      901 aacctgacct accgagatct ctattacttc atctttgctc ctactttgtg ttatgaactc
      961 aactttcctc gatccccccg aatacgaaag cgctttctgc tacggcgggt tcttgagatg
     1021 ctctttttca cccagcttca agtggggctg atccagcagt ggatggtccc tactatccag
     1081 aactccatga agcccttcaa ggacatggac tattcacgaa tcattgagcg tctcttaaag
     1141 ctggcggtcc ccaaccatct gatatggctc atcttcttct attggctttt ccactcatgt
     1201 ctcaatgctg tggcagagct cctgcagttt ggagaccgcg agttctacag ggactggtgg
     1261 aatgctgagt ctgtcaccta cttttggcag aactggaata tccccgtgca caagtggtgc
     1321 atcagacact tctacaagcc tatgctcaga ctgggcagca acaaatggat ggccaggact
     1381 ggggtctttt tggcgtcagc cttcttccat gagtacctag tgagcattcc cctgaggatg
     1441 ttccgcctct gggcattcac agccatgatg gctcaggtcc cactggcctg gattgtgaac
     1501 cgcttcttcc aagggaacta tggcaatgca gctgtgtggg tgacactcat cattgggcaa
     1561 ccggtggctg tgctcatgta tgtccacgac tactacgtgc tcaactatga tgccccagta
     1621 ggggcctgag ctactgccaa aggccagccc tccctaacct gggcctggag ttctggaggg
     1681 cttcctggct gcctgcacac tcctcctagt ctgggaggcc tctctgcccc tatggggcct
     1741 actcctgctc ttggggatgg cacctgagtc cagctggtat gagccagtgc tgggagtctg
     1801 tgctgaccag gggctgagga tatcaataaa gagctatcta aaaaccttcc tacaggggtt
     1861 gtagaggctg ctgaggatga
//
