LOCUS       NM_053362               1324 bp    mRNA    linear   ROD 03-APR-2024
DEFINITION  Rattus norvegicus DNA fragmentation factor subunit beta (Dffb),
            mRNA.
ACCESSION   NM_053362
VERSION     NM_053362.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 1324)
  AUTHORS   Iglesias-Guimarais,V., Gil-Guinon,E., Gabernet,G.,
            Garcia-Belinchon,M., Sanchez-Osuna,M., Casanelles,E., Comella,J.X.
            and Yuste,V.J.
  TITLE     Apoptotic DNA degradation into oligonucleosomal fragments, but not
            apoptotic nuclear morphology, relies on a cytosolic pool of
            DFF40/CAD endonuclease
  JOURNAL   J Biol Chem 287 (10), 7766-7779 (2012)
   PUBMED   22253444
REFERENCE   2  (bases 1 to 1324)
  AUTHORS   Ninios,Y.P., Sekeri-Pataryas,K.E. and Sourlingas,T.G.
  TITLE     Histone H1 subtype preferences of DFF40 and possible nuclear
            localization of DFF40/45 in normal and trichostatin A-treated NB4
            leukemic cells
  JOURNAL   Apoptosis 15 (2), 128-138 (2010)
   PUBMED   19882353
REFERENCE   3  (bases 1 to 1324)
  AUTHORS   Park,H.H.
  TITLE     Fifty C-terminal amino acid residues are necessary for the
            chaperone activity of DFF45 but not for the inhibition of DFF40
  JOURNAL   BMB Rep 42 (11), 713-718 (2009)
   PUBMED   19944011
REFERENCE   4  (bases 1 to 1324)
  AUTHORS   Ahn,J.Y., Liu,X., Liu,Z., Pereira,L., Cheng,D., Peng,J., Wade,P.A.,
            Hamburger,A.W. and Ye,K.
  TITLE     Nuclear Akt associates with PKC-phosphorylated Ebp1, preventing DNA
            fragmentation by inhibition of caspase-activated DNase
  JOURNAL   EMBO J 25 (10), 2083-2095 (2006)
   PUBMED   16642037
  REMARK    GeneRIF: Results suggest that Ebp1 contributes to inhibition of DNA
            fragmentation by caspase-activated DNase, and that nuclear Akt
            might contribute to suppressing apoptosis through interaction with
            Ebp1.
REFERENCE   5  (bases 1 to 1324)
  AUTHORS   Henne,W.M., Oomman,S., Attridge,J., Finckbone,V., Coates,P.,
            Bliss,R., Strahlendorf,H. and Strahlendorf,J.
  TITLE     AMPA-induced excitotoxicity increases nuclear levels of CAD,
            endonuclease G, and acinus and induces chromatin condensation in
            rat hippocampal pyramidal neurons
  JOURNAL   Cell Mol Neurobiol 26 (3), 321-339 (2006)
   PUBMED   16767516
  REMARK    GeneRIF: Data indicate increases in the nuclear levels of
            caspase-activated acinus and DNase and Endonuclease G (a
            caspase-independent endonuclease) in CA1 and CA3 pyramidal cell
            nuclei with different temporal patterns following an AMPA-insult.
REFERENCE   6  (bases 1 to 1324)
  AUTHORS   Shiokawa,D. and Tanuma,S.
  TITLE     Differential DNases are selectively used in neuronal apoptosis
            depending on the differentiation state
  JOURNAL   Cell Death Differ 11 (10), 1112-1120 (2004)
   PUBMED   15167901
  REMARK    GeneRIF: DNA fragmentation in neuronal apoptosis is catalyzed by
            either CAD or DNase gamma depending on the differentiation state
REFERENCE   7  (bases 1 to 1324)
  AUTHORS   Cao,G., Pei,W., Lan,J., Stetler,R.A., Luo,Y., Nagayama,T.,
            Graham,S.H., Yin,X.M., Simon,R.P. and Chen,J.
  TITLE     Caspase-activated DNase/DNA fragmentation factor 40 mediates
            apoptotic DNA fragmentation in transient cerebral ischemia and in
            neuronal cultures
  JOURNAL   J Neurosci 21 (13), 4678-4690 (2001)
   PUBMED   11425895
REFERENCE   8  (bases 1 to 1324)
  AUTHORS   Zhou,P., Lugovskoy,A.A., McCarty,J.S., Li,P. and Wagner,G.
  TITLE     Solution structure of DFF40 and DFF45 N-terminal domain complex and
            mutual chaperone activity of DFF40 and DFF45
  JOURNAL   Proc Natl Acad Sci U S A 98 (11), 6051-6055 (2001)
   PUBMED   11371636
REFERENCE   9  (bases 1 to 1324)
  AUTHORS   Durrieu,F., Samejima,K., Fortune,J.M., Kandels-Lewis,S.,
            Osheroff,N. and Earnshaw,W.C.
  TITLE     DNA topoisomerase IIalpha interacts with CAD nuclease and is
            involved in chromatin condensation during apoptotic execution
  JOURNAL   Curr Biol 10 (15), 923-926 (2000)
   PUBMED   10959840
REFERENCE   10 (bases 1 to 1324)
  AUTHORS   Liu,X., Zou,H., Slaughter,C. and Wang,X.
  TITLE     DFF, a heterodimeric protein that functions downstream of caspase-3
            to trigger DNA fragmentation during apoptosis
  JOURNAL   Cell 89 (2), 175-184 (1997)
   PUBMED   9108473
COMMENT     PROVISIONAL REFSEQ: This record has not yet been subject to final
            NCBI review. The reference sequence was derived from
            JAXUCZ010000005.1.
            
            On Nov 26, 2020 this sequence version replaced NM_053362.1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AF136598.1, SRR26643292.24492.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN12840120 [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-137               JAXUCZ010000005.1  169816844-169816980 c
            138-264             JAXUCZ010000005.1  169816546-169816672 c
            265-453             JAXUCZ010000005.1  169814959-169815147 c
            454-533             JAXUCZ010000005.1  169813040-169813119 c
            534-704             JAXUCZ010000005.1  169811075-169811245 c
            705-805             JAXUCZ010000005.1  169810043-169810143 c
            806-1324            JAXUCZ010000005.1  169805664-169806182 c
FEATURES             Location/Qualifiers
     source          1..1324
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="BN"
                     /db_xref="taxon:10116"
                     /chromosome="5"
                     /map="5q36"
     gene            1..1324
                     /gene="Dffb"
                     /gene_synonym="Cad"
                     /note="DNA fragmentation factor subunit beta"
                     /db_xref="GeneID:84359"
                     /db_xref="RGD:620335"
     exon            1..137
                     /gene="Dffb"
                     /gene_synonym="Cad"
                     /inference="alignment:Splign:2.1.0"
     CDS             15..1064
                     /gene="Dffb"
                     /gene_synonym="Cad"
                     /note="DFF-40; caspase-activated deoxyribonuclease; DNA
                     fragmentation factor 40 kDa subunit; caspase-activated
                     DNase; DNA fragmentation factor, 40 kD, beta polypeptide
                     (caspase-activated DNase); DNA fragmentation factor, beta
                     subunit; DNA fragmentation factor, 40kDa, beta polypeptide
                     (caspase-activated DNase); DNA fragmentation factor, beta
                     polypeptide (caspase-activated DNase)"
                     /codon_start=1
                     /product="DNA fragmentation factor subunit beta"
                     /protein_id="NP_445814.1"
                     /db_xref="GeneID:84359"
                     /db_xref="RGD:620335"
                     /translation="MCAVLPQPKCVKLRALHSSCKFGVAARSCQELLRKGCIRFQLPV
                     PGSRLCMYEDGTEVTDDCFPSLPNDSELLLLTAGETWHGYVSDITRLLSVFNEPHAGV
                     IQAARQLLSDEQAPLRQKLLADLLHHVSQNITAETREQDPSWFEGLESRFRNKSGYLR
                     YSCESRIRGYLREVSAYISMVDAAAREEYLRVLSSMCHKLKSVQYNGSYFDRGAEASS
                     RLCTPEGWFSCQGPFDLESCLSKHSINPYGNRESRILFSTWNLDHIIEKKRTVVPTLA
                     EAIQDGREVNWEYFYSLLFTAENLKLVHIACHKKTTHKLQCDRSRIYRPQTGSRRKQP
                     PRKQPPRKRPPRKRQ"
     exon            138..264
                     /gene="Dffb"
                     /gene_synonym="Cad"
                     /inference="alignment:Splign:2.1.0"
     exon            265..453
                     /gene="Dffb"
                     /gene_synonym="Cad"
                     /inference="alignment:Splign:2.1.0"
     exon            454..533
                     /gene="Dffb"
                     /gene_synonym="Cad"
                     /inference="alignment:Splign:2.1.0"
     exon            534..704
                     /gene="Dffb"
                     /gene_synonym="Cad"
                     /inference="alignment:Splign:2.1.0"
     exon            705..805
                     /gene="Dffb"
                     /gene_synonym="Cad"
                     /inference="alignment:Splign:2.1.0"
     exon            806..1324
                     /gene="Dffb"
                     /gene_synonym="Cad"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 acggcagctc tgcgatgtgc gcggtgctcc cccaacccaa atgcgtcaag ttgcgagctc
       61 tacacagctc gtgcaagttc ggcgtggcgg cccggagctg ccaggagctg ctgcgcaagg
      121 gttgtatccg cttccagctc ccggtgcccg gctcccggct gtgcatgtac gaagatggca
      181 cggaggtgac cgacgactgc ttcccgagcc ttcccaacga ctctgagctc ctattgctca
      241 ccgctggaga gacctggcac ggctatgtga gtgacatcac acgtctcctc agtgtgttta
      301 acgagccaca tgccggggtc atccaggctg cgcggcaact gctgtcagat gagcaggccc
      361 cactgaggca gaagctgctg gccgaccttc tgcatcatgt gagccagaat attactgcag
      421 agacccggga acaggaccca tcctggtttg aaggtttgga gtctcgattc aggaataagt
      481 ctggctacct gagatacagc tgtgagagcc ggatccgggg ttacctgaga gaggtgagcg
      541 cttacatctc aatggtggat gcagcagccc gggaagagta cctgcgggtc ctcagctcca
      601 tgtgccacaa gctcaagtcc gtgcagtaca acggcagcta tttcgacaga ggtgcagagg
      661 ccagcagccg cctttgtacc ccagaaggct ggttctcctg ccagggcccc tttgacttgg
      721 agagctgtct ctccaaacac tccatcaacc cctatggcaa cagagagagc cggatcctct
      781 tcagtacctg gaacctggac catataatag agaagaagcg caccgtggtg cccacgctgg
      841 ctgaagcaat ccaggacggg agggaagtga actgggagta cttctacagc ctgctcttca
      901 ctgccgagaa cctgaagcta gtgcacatcg cctgccacaa gaagaccaca cacaagctgc
      961 agtgcgaccg cagcaggatc tatcggcctc agacaggatc caggaggaag cagccccctc
     1021 ggaagcagcc ccctcggaag cgcccccctc ggaaacgcca gtgacacgtg ccatgccctc
     1081 tttgaggggg ctgcagtggt aatgggggcc attatgcttc atgctttgtc actccagaag
     1141 attcaaggaa agtctttagg aatctttcca aaggatctgg tttgtgtgcc tcctgtttgc
     1201 ttccccagcc tctgacagcc cagtgctcac tgtgttccca tggcagggtc ttcatgctca
     1261 tggccccagg gctgaactct gccactgtga tatgagtggg agctgctctg tgctgagctt
     1321 ttat
//
