LOCUS       NM_001398977            2407 bp    mRNA    linear   ROD 27-MAR-2024
DEFINITION  Rattus norvegicus DNA fragmentation factor subunit alpha (Dffa),
            transcript variant 1, mRNA.
ACCESSION   NM_001398977 XM_006239362
VERSION     NM_001398977.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 2407)
  AUTHORS   Iglesias-Guimarais,V., Gil-Guinon,E., Gabernet,G.,
            Garcia-Belinchon,M., Sanchez-Osuna,M., Casanelles,E., Comella,J.X.
            and Yuste,V.J.
  TITLE     Apoptotic DNA degradation into oligonucleosomal fragments, but not
            apoptotic nuclear morphology, relies on a cytosolic pool of
            DFF40/CAD endonuclease
  JOURNAL   J Biol Chem 287 (10), 7766-7779 (2012)
   PUBMED   22253444
REFERENCE   2  (bases 1 to 2407)
  AUTHORS   Ninios,Y.P., Sekeri-Pataryas,K.E. and Sourlingas,T.G.
  TITLE     Histone H1 subtype preferences of DFF40 and possible nuclear
            localization of DFF40/45 in normal and trichostatin A-treated NB4
            leukemic cells
  JOURNAL   Apoptosis 15 (2), 128-138 (2010)
   PUBMED   19882353
REFERENCE   3  (bases 1 to 2407)
  AUTHORS   Park,H.H.
  TITLE     Fifty C-terminal amino acid residues are necessary for the
            chaperone activity of DFF45 but not for the inhibition of DFF40
  JOURNAL   BMB Rep 42 (11), 713-718 (2009)
   PUBMED   19944011
REFERENCE   4  (bases 1 to 2407)
  AUTHORS   Korn,C., Scholz,S.R., Gimadutdinow,O., Lurz,R., Pingoud,A. and
            Meiss,G.
  TITLE     Interaction of DNA fragmentation factor (DFF) with DNA reveals an
            unprecedented mechanism for nuclease inhibition and suggests that
            DFF can be activated in a DNA-bound state
  JOURNAL   J Biol Chem 280 (7), 6005-6015 (2005)
   PUBMED   15572351
REFERENCE   5  (bases 1 to 2407)
  AUTHORS   Zhang,J., Dong,M., Li,L., Fan,Y., Pathre,P., Dong,J., Lou,D.,
            Wells,J.M., Olivares-Villagomez,D., Van Kaer,L., Wang,X. and Xu,M.
  TITLE     Endonuclease G is required for early embryogenesis and normal
            apoptosis in mice
  JOURNAL   Proc Natl Acad Sci U S A 100 (26), 15782-15787 (2003)
   PUBMED   14663139
REFERENCE   6  (bases 1 to 2407)
  AUTHORS   Zhou,P., Lugovskoy,A.A., McCarty,J.S., Li,P. and Wagner,G.
  TITLE     Solution structure of DFF40 and DFF45 N-terminal domain complex and
            mutual chaperone activity of DFF40 and DFF45
  JOURNAL   Proc Natl Acad Sci U S A 98 (11), 6051-6055 (2001)
   PUBMED   11371636
REFERENCE   7  (bases 1 to 2407)
  AUTHORS   Chen,D., Stetler,R.A., Cao,G., Pei,W., O'Horo,C., Yin,X.M. and
            Chen,J.
  TITLE     Characterization of the rat DNA fragmentation factor 35/Inhibitor
            of caspase-activated DNase (Short form). The endogenous inhibitor
            of caspase-dependent DNA fragmentation in neuronal apoptosis
  JOURNAL   J Biol Chem 275 (49), 38508-38517 (2000)
   PUBMED   10984476
REFERENCE   8  (bases 1 to 2407)
  AUTHORS   Zhang,J., Wang,X., Bove,K.E. and Xu,M.
  TITLE     DNA fragmentation factor 45-deficient cells are more resistant to
            apoptosis and exhibit different dying morphology than wild-type
            control cells
  JOURNAL   J Biol Chem 274 (52), 37450-37454 (1999)
   PUBMED   10601318
REFERENCE   9  (bases 1 to 2407)
  AUTHORS   Inohara,N., Koseki,T., Chen,S., Wu,X. and Nunez,G.
  TITLE     CIDE, a novel family of cell death activators with homology to the
            45 kDa subunit of the DNA fragmentation factor
  JOURNAL   EMBO J 17 (9), 2526-2533 (1998)
   PUBMED   9564035
REFERENCE   10 (bases 1 to 2407)
  AUTHORS   Liu,X., Zou,H., Slaughter,C. and Wang,X.
  TITLE     DFF, a heterodimeric protein that functions downstream of caspase-3
            to trigger DNA fragmentation during apoptosis
  JOURNAL   Cell 89 (2), 175-184 (1997)
   PUBMED   9108473
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            JAXUCZ010000005.1.
            
            On Dec 29, 2021 this sequence version replaced XM_006239362.4.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR26643284.8337.1,
                                           SRR26643291.16772.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA5760383, SAMEA5760386
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-227               JAXUCZ010000005.1  164823807-164824033
            228-389             JAXUCZ010000005.1  164825647-164825808
            390-532             JAXUCZ010000005.1  164826940-164827082
            533-722             JAXUCZ010000005.1  164833729-164833918
            723-874             JAXUCZ010000005.1  164834100-164834251
            875-2407            JAXUCZ010000005.1  164835197-164836729
FEATURES             Location/Qualifiers
     source          1..2407
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="BN"
                     /db_xref="taxon:10116"
                     /chromosome="5"
                     /map="5q36"
     gene            1..2407
                     /gene="Dffa"
                     /gene_synonym="ICAD-S"
                     /note="DNA fragmentation factor subunit alpha"
                     /db_xref="GeneID:114214"
                     /db_xref="RGD:620334"
     exon            1..227
                     /gene="Dffa"
                     /gene_synonym="ICAD-S"
                     /inference="alignment:Splign:2.1.0"
     CDS             92..1078
                     /gene="Dffa"
                     /gene_synonym="ICAD-S"
                     /note="isoform 1 is encoded by transcript variant 1; DNA
                     fragmentation factor, alpha subunit; DNA fragmentation
                     factor, alpha polypeptide"
                     /codon_start=1
                     /product="DNA fragmentation factor subunit alpha isoform
                     1"
                     /protein_id="NP_001385906.1"
                     /db_xref="GeneID:114214"
                     /db_xref="RGD:620334"
                     /translation="MELSRGASAPDPDDVGPLKPCLLRRNHSREQHGVAASSLEELRS
                     KACELLAIDKSLTPVTLVLAEDGTIVDDEDYFLCLPSNTKFVALACNEKWAYNDSDGG
                     TAWLSQESFDTDQTDSGAGVKWKNVARQLKEDLSSIILLSEEELQALIDIPCAELAQE
                     LCQSCATVQGLQSTLQQVLDQREEARQSKQLLELYLQALEKEGSILSNQNESKAALGE
                     ELDAVDTGVGGEVASEVMLRSQILAALKEKPAPELSLSSQDLESVSKEDPKALAVALS
                     WDIKKAETVQQACTTELALRLQQVQSLHSLRNLSAKRKPLPGDPQSPKRAKV"
     exon            228..389
                     /gene="Dffa"
                     /gene_synonym="ICAD-S"
                     /inference="alignment:Splign:2.1.0"
     exon            390..532
                     /gene="Dffa"
                     /gene_synonym="ICAD-S"
                     /inference="alignment:Splign:2.1.0"
     exon            533..722
                     /gene="Dffa"
                     /gene_synonym="ICAD-S"
                     /inference="alignment:Splign:2.1.0"
     exon            723..874
                     /gene="Dffa"
                     /gene_synonym="ICAD-S"
                     /inference="alignment:Splign:2.1.0"
     exon            875..2407
                     /gene="Dffa"
                     /gene_synonym="ICAD-S"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 gtcaagccgc gagaggaggg agtggcaaag cttagggaag ccgctctggg ctacctggga
       61 tttcccagag tcgccaagtc ccactttgag gatggagcta tcccggggag ccagcgcccc
      121 ggacccggac gatgtcgggc ctctcaaacc gtgtctgctt cgccgcaacc acagccgtga
      181 gcagcacggc gtggcagcct ccagtctcga ggagctgagg agcaaagcct gtgaacttct
      241 ggccatcgat aagtccctga caccagtcac cctggtcctg gcagaggatg ggaccatagt
      301 ggacgatgag gactatttcc tctgtctccc ttccaacaca aagtttgtgg cactggcctg
      361 caacgagaag tgggcataca acgactcgga tggaggaaca gcttggcttt cccaagagtc
      421 ctttgacaca gatcaaacgg acagtggggc aggggtgaag tggaagaatg tggccaggca
      481 gctgaaagag gatctgtcca gcatcatcct gctgtcagaa gaggagctcc aagcactcat
      541 cgacatcccc tgtgcagagc tggcgcagga actctgccag agttgtgcca ctgtccaggg
      601 gctgcagagc acgctccagc aggtgcttga ccagagagag gaagcacgcc agtccaagca
      661 gctcctggaa ctttatctcc aggccttgga gaaagagggc agcatcttgt ccaaccagaa
      721 cgagtccaaa gctgcccttg gggaagagct ggatgcagtt gacaccggcg tcggcggaga
      781 ggtggcttca gaagtgatgc tgagaagcca gatcctcgca gccctgaagg agaagccggc
      841 tccagagctg agtctgtcta gtcaggattt agagtcggtc tccaaggagg accccaaagc
      901 cctggctgtc gctctgagct gggacataaa gaaggcagag acagtccagc aggcctgtac
      961 cacggagctc gccctgcgcc tgcagcaagt gcagagcttg cactcactca ggaatctttc
     1021 tgcaaagagg aagccactgc ctggggaccc gcagagtccc aaacgggcca aagtgtgaga
     1081 cttctcatag ccaaaccgga caagctttgc agaggagaac ctcggttctg aggtccgtaa
     1141 acacctgtct gtgaccttgt gacctcgtga cctcgttccc acccgcactc cctccacctc
     1201 ccgccctgcg gtgagctctc ctcgctccag aaaagctgtt ccctgtctgt gcatacccct
     1261 ctacctcctt tcaatgtcct gggttttcgt tctgttttct tacctgtgtg ggtgtgttgc
     1321 ctgcacgtat atctgtatcc ctcatgccca tgaaggccag aagagagtgt cgggtccctt
     1381 ggagctggag taacagacag ctctcagcag ccgtgtgggt gctgggaatc gactctgggt
     1441 cctctggaag tggggccggt gctcttagcc cctgagccat ctccagctcc cagaatgcac
     1501 ttatcgtgtg ctggccttag gttaagtgaa agtacccgtc tagggcaatg aaaacctgtc
     1561 atactttgga ctcgatgtcc ttactctaca acttgaacaa tagagcgtta gacacagtgg
     1621 tagaaaaaag gcacccaaca gcagcagtgg tggccatgaa cttccttctc aagttaatga
     1681 atgcagttgc tgtccgctta aaggcacata ctgagtctgc agaccccagg gacatggagt
     1741 ctaagccccc atttctaata tctgactcag tgttgtgaag ctgttaccgc tgtgagactc
     1801 cagtccattt tctaatatgt tttgccagca gggggtagaa aggatcattg agtcccatgc
     1861 tctgcgactc aggtttctct ctgcagatca ggtcacttcc agcttttctc tgctgtttct
     1921 gctcagagtt ctacaccttc tgcaatcgac agtgctgtct gccttcaggg aacggacacc
     1981 tagttacggg tcatctgact ttcatgcgtg ctgggaagaa gaagagggtc tctagctctc
     2041 gtcttttttt ttcgtgtgtg tgtgtgtgtg tgtgtgtgtg tgtgtgtgtg tgtgtgtgtg
     2101 cgtgtgtttt ctgggtactg ggaattgaac taagatttta ttcatagaca ctgagctcgt
     2161 gccccctaat gaaccacacc ccagcctcac gttgacaaag aaatatagca ttaagtcttg
     2221 gggtaaaggt ggggtgcacg aagcaaataa aagtaattgt gtccttgcct acaggggggg
     2281 cctggtgcaa ctttcagatc ttgtagtgcc tgacagcacc agttccaaat gttggcatca
     2341 aagctcttcc cacatctttg tggtgcctct cagtccctga ataaaagcca ttgctgatca
     2401 tccatca
//
