LOCUS       NP_001071115             713 aa            linear   ROD 29-MAR-2024
DEFINITION  probable ATP-dependent RNA helicase DDX4 [Rattus norvegicus].
ACCESSION   NP_001071115 XP_001067709 XP_001074861
VERSION     NP_001071115.2
DBSOURCE    REFSEQ: accession NM_001077647.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (residues 1 to 713)
  AUTHORS   Ozcan,P., Takmaz,T., Tok,O.E., Islek,S., Yigit,E.N. and
            Ficicioglu,C.
  TITLE     The protective effect of platelet-rich plasma administrated on
            ovarian function in female rats with Cy-induced ovarian damage
  JOURNAL   J Assist Reprod Genet 37 (4), 865-873 (2020)
   PUBMED   32020412
  REMARK    GeneRIF: The protective effect of platelet-rich plasma
            administrated on ovarian function in female rats with Cy-induced
            ovarian damage.
REFERENCE   2  (residues 1 to 713)
  AUTHORS   Spade,D.J., Hall,S.J., Wortzel,J.D., Reyes,G. and Boekelheide,K.
  TITLE     All-trans Retinoic Acid Disrupts Development in Ex Vivo Cultured
            Fetal Rat Testes. II: Modulation of Mono-(2-ethylhexyl) Phthalate
            Toxicity
  JOURNAL   Toxicol Sci 168 (1), 149-159 (2019)
   PUBMED   30476341
REFERENCE   3  (residues 1 to 713)
  AUTHORS   Spade,D.J., Dere,E., Hall,S.J., Schorl,C., Freiman,R.N. and
            Boekelheide,K.
  TITLE     All-Trans Retinoic Acid Disrupts Development in Ex Vivo Cultured
            Fetal Rat Testes. I: Altered Seminiferous Cord Maturation and
            Testicular Cell Fate
  JOURNAL   Toxicol Sci 167 (2), 546-558 (2019)
   PUBMED   30329139
REFERENCE   4  (residues 1 to 713)
  AUTHORS   Wenda,J.M., Homolka,D., Yang,Z., Spinelli,P., Sachidanandam,R.,
            Pandey,R.R. and Pillai,R.S.
  TITLE     Distinct Roles of RNA Helicases MVH and TDRD9 in PIWI
            Slicing-Triggered Mammalian piRNA Biogenesis and Function
  JOURNAL   Dev Cell 41 (6), 623-637 (2017)
   PUBMED   28633017
REFERENCE   5  (residues 1 to 713)
  AUTHORS   Kawahara,C., Yokota,S. and Fujita,H.
  TITLE     DDX6 localizes to nuage structures and the annulus of mammalian
            spermatogenic cells
  JOURNAL   Histochem Cell Biol 141 (1), 111-121 (2014)
   PUBMED   24141902
REFERENCE   6  (residues 1 to 713)
  AUTHORS   Kuramochi-Miyagawa,S., Kimura,T., Ijiri,T.W., Isobe,T., Asada,N.,
            Fujita,Y., Ikawa,M., Iwai,N., Okabe,M., Deng,W., Lin,H., Matsuda,Y.
            and Nakano,T.
  TITLE     Mili, a mammalian member of piwi family gene, is essential for
            spermatogenesis
  JOURNAL   Development 131 (4), 839-849 (2004)
   PUBMED   14736746
REFERENCE   7  (residues 1 to 713)
  AUTHORS   McClellan,K.A., Gosden,R. and Taketo,T.
  TITLE     Continuous loss of oocytes throughout meiotic prophase in the
            normal mouse ovary
  JOURNAL   Dev Biol 258 (2), 334-348 (2003)
   PUBMED   12798292
REFERENCE   8  (residues 1 to 713)
  AUTHORS   Toyooka,Y., Tsunekawa,N., Takahashi,Y., Matsui,Y., Satoh,M. and
            Noce,T.
  TITLE     Expression and intracellular localization of mouse Vasa-homologue
            protein during germ cell development
  JOURNAL   Mech Dev 93 (1-2), 139-149 (2000)
   PUBMED   10781947
REFERENCE   9  (residues 1 to 713)
  AUTHORS   Tanaka,S.S., Toyooka,Y., Akasu,R., Katoh-Fukui,Y., Nakahara,Y.,
            Suzuki,R., Yokoyama,M. and Noce,T.
  TITLE     The mouse homolog of Drosophila Vasa is required for the
            development of male germ cells
  JOURNAL   Genes Dev 14 (7), 841-853 (2000)
   PUBMED   10766740
REFERENCE   10 (residues 1 to 713)
  AUTHORS   Komiya,T. and Tanigawa,Y.
  TITLE     Cloning of a gene of the DEAD box protein family which is
            specifically expressed in germ cells in rats
  JOURNAL   Biochem Biophys Res Commun 207 (1), 405-410 (1995)
   PUBMED   7857296
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            JAXUCZ010000002.1.
            
            On Feb 18, 2021 this sequence version replaced NP_001071115.1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR26643293.10427.1,
                                           SRR26360181.3185612.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA5760383,
                                           SAMEA5760389 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation
            ##RefSeq-Attributes-END##
FEATURES             Location/Qualifiers
     source          1..713
                     /organism="Rattus norvegicus"
                     /strain="BN"
                     /db_xref="taxon:10116"
                     /chromosome="2"
                     /map="2q14"
     Protein         1..713
                     /product="probable ATP-dependent RNA helicase DDX4"
                     /EC_number="3.6.4.13"
                     /note="vasa-like gene protein; vasa homolog; DEAD box
                     protein 4; putative ATP-dependent RNA helicase DDX4; DEAD
                     (Asp-Glu-Ala-Asp) box polypeptide 4"
                     /calculated_mol_wt=77789
     Region          23..240
                     /region_name="Disordered.
                     /evidence=ECO:0000256|SAM:MobiDB-lite"
                     /note="propagated from UniProtKB/Swiss-Prot (Q64060.1)"
     Site            207
                     /site_type="phosphorylation"
                     /note="Phosphoserine.
                     /evidence=ECO:0007744|PubMed:22673903; propagated from
                     UniProtKB/Swiss-Prot (Q64060.1)"
     Site            211
                     /site_type="phosphorylation"
                     /note="Phosphoserine.
                     /evidence=ECO:0000250|UniProtKB:Q61496; propagated from
                     UniProtKB/Swiss-Prot (Q64060.1)"
     Region          213..232
                     /region_name="Interaction with RANBP9.
                     /evidence=ECO:0000250|UniProtKB:Q9NQI0"
                     /note="propagated from UniProtKB/Swiss-Prot (Q64060.1)"
     Region          242..494
                     /region_name="DEADc_DDX4"
                     /note="DEAD-box helicase domain of DEAD box protein 4;
                     cd18052"
                     /db_xref="CDD:350810"
     Site            order(253,263,275,293,295..297,300,318..324,431..432,467)
                     /site_type="other"
                     /note="ATP binding site [chemical binding]"
                     /db_xref="CDD:350810"
     Region          273..301
                     /region_name="Q motif"
                     /note="propagated from UniProtKB/Swiss-Prot (Q64060.1)"
     Site            order(358..360,386..387,407..410,413,435,441)
                     /site_type="other"
                     /note="RNA binding site [nucleotide binding]"
                     /db_xref="CDD:350810"
     Region          431..434
                     /region_name="DEAD box.
                     /evidence=ECO:0000250|UniProtKB:Q61496"
                     /note="propagated from UniProtKB/Swiss-Prot (Q64060.1)"
     Region          500..629
                     /region_name="SF2_C_DEAD"
                     /note="C-terminal helicase domain of the DEAD box
                     helicases; cd18787"
                     /db_xref="CDD:350174"
     Site            order(588,590,615,618..619)
                     /site_type="other"
                     /note="ATP binding site [chemical binding]"
                     /db_xref="CDD:350174"
     Region          689..713
                     /region_name="Disordered.
                     /evidence=ECO:0000256|SAM:MobiDB-lite"
                     /note="propagated from UniProtKB/Swiss-Prot (Q64060.1)"
     Site            711
                     /site_type="phosphorylation"
                     /note="Phosphoserine.
                     /evidence=ECO:0000250|UniProtKB:Q61496; propagated from
                     UniProtKB/Swiss-Prot (Q64060.1)"
     CDS             1..713
                     /gene="Ddx4"
                     /gene_synonym="RVLG"
                     /coded_by="NM_001077647.2:273..2414"
                     /db_xref="GeneID:310090"
                     /db_xref="RGD:1308793"
ORIGIN      
        1 mgdedweaei lkphvssyvp vfekdkyssg angdtfnrts asssemedgp sgrdhfmrsg
       61 fssgrnlgnr digesskret tsttggfgrg kgfgnrgfln nkfeegdssg fwkestndce
      121 dtqtrsrgfs krggypdgnd seasgpfrrg grdseydqdq gsqrggglfg srkpaasdsg
      181 sgdtfqsrsg ngrggykgln eevvtgsgkn swkseaegge ssdiqgpkvt yipppppede
      241 dsifahyqtg infdkydtil vevsghdapp ailtfeeanl cqtlnnniak agytkltpvq
      301 kysipivlag rdlmacaqtg sgktaafllp ilahmmrdgi tasrfkelqe peciivaptr
      361 elinqiylea rkfsfgtcvr avviyggtqf ghsirqivqg cnilcatpgr lmdiigkeki
      421 glkqvkylvl deadrmldmg fgpemkklis cpgmpskeqr qtllfsatfp eeiqrlagef
      481 lksnylfvav gqvggacrdv qqsilqvgqy skreklveil rnigdertmv fvetkkkadf
      541 iatflcqeki sttsihgdre qrereqalgd frcgkcpvlv atsvaargld ienvqhvinf
      601 dlpstideyv hrigrtgrcg ntgraisffd tesdnhlaqp lvkvlsdaqq dvpawleeia
      661 fssyappsfs nstrgavfas vdtrknfqgk ntlntagiss aqapnpvdde swd
//
