LOCUS       NM_031630               1630 bp    mRNA    linear   ROD 02-JUN-2024
DEFINITION  Rattus norvegicus DEAD-box helicase 25 (Ddx25), mRNA.
ACCESSION   NM_031630
VERSION     NM_031630.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 1630)
  AUTHORS   Onohara,Y. and Yokota,S.
  TITLE     Expression of DDX25 in nuage components of mammalian spermatogenic
            cells: immunofluorescence and immunoelectron microscopic study
  JOURNAL   Histochem Cell Biol 137 (1), 37-51 (2012)
   PUBMED   22038044
  REMARK    GeneRIF: The results suggest that DDX25 functions in meiotic
            cells,intermitochondrial cement,loose aggregates of
            particles,chromatoid bodies,late chromatoid bodies,satellite
            bodies,granulated bodies,mitochondria-associated granules and
            reticulated bodies.
REFERENCE   2  (bases 1 to 1630)
  AUTHORS   Dufau,M.L., Sato,H., Gutti,R. and Tsai-Morris,C.H.
  TITLE     Gonadotropin-regulated testicular helicase (GRTH/DDX25): a master
            post-transcriptional regulator of spermatogenesis
  JOURNAL   Adv Exp Med Biol 707, 23-29 (2011)
   PUBMED   21691948
  REMARK    GeneRIF: studies on GRTH [proide insights into the intrinsic
            requirements of spermatogenesis and open new avenues for studies on
            regulating spermatogenesis, infertility and male contraception
REFERENCE   3  (bases 1 to 1630)
  AUTHORS   Sheng,Y., Tsai-Morris,C.H., Gutti,R., Maeda,Y. and Dufau,M.L.
  TITLE     Gonadotropin-regulated testicular RNA helicase (GRTH/Ddx25) is a
            transport protein involved in gene-specific mRNA export and protein
            translation during spermatogenesis
  JOURNAL   J Biol Chem 281 (46), 35048-35056 (2006)
   PUBMED   16968703
  REMARK    GeneRIF: The results of this study indicate that the mammalian
            GRTH/Ddx25 gene is a multifunctional RNA helicase that is an
            essential regulator of sperm maturation.
REFERENCE   4  (bases 1 to 1630)
  AUTHORS   Tsai-Morris,C.H., Sheng,Y., Lee,E., Lei,K.J. and Dufau,M.L.
  TITLE     Gonadotropin-regulated testicular RNA helicase (GRTH/Ddx25) is
            essential for spermatid development and completion of
            spermatogenesis
  JOURNAL   Proc Natl Acad Sci U S A 101 (17), 6373-6378 (2004)
   PUBMED   15096601
REFERENCE   5  (bases 1 to 1630)
  AUTHORS   Sheng,Y., Tsai-Morris,C.H. and Dufau,M.L.
  TITLE     Cell-specific and hormone-regulated expression of
            gonadotropin-regulated testicular RNA helicase gene (GRTH/Ddx25)
            resulting from alternative utilization of translation initiation
            codons in the rat testis
  JOURNAL   J Biol Chem 278 (30), 27796-27803 (2003)
   PUBMED   12734186
  REMARK    GeneRIF: expression is regulated by gonadotropin/androgen at the
            translational level
REFERENCE   6  (bases 1 to 1630)
  AUTHORS   Tang,P.Z., Tsai-Morris,C.H. and Dufau,M.L.
  TITLE     A novel gonadotropin-regulated testicular RNA helicase. A new
            member of the dead-box family
  JOURNAL   J Biol Chem 274 (53), 37932-37940 (1999)
   PUBMED   10608860
COMMENT     PROVISIONAL REFSEQ: This record has not yet been subject to final
            NCBI review. The reference sequence was derived from AF142629.2.
            
            On Jul 24, 2003 this sequence version replaced NM_031630.1.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AF142629.2, BC078791.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA5760383, SAMEA5760389
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
FEATURES             Location/Qualifiers
     source          1..1630
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="Sprague-Dawley"
                     /db_xref="taxon:10116"
                     /chromosome="8"
                     /map="8q21"
     gene            1..1630
                     /gene="Ddx25"
                     /gene_synonym="GRTH"
                     /note="DEAD-box helicase 25"
                     /db_xref="GeneID:58856"
                     /db_xref="RGD:68381"
     exon            1..88
                     /gene="Ddx25"
                     /gene_synonym="GRTH"
                     /inference="alignment:Splign:2.1.0"
     CDS             26..1477
                     /gene="Ddx25"
                     /gene_synonym="GRTH"
                     /EC_number="3.6.4.13"
                     /note="DEAD box protein 25; gonadotropin-regulated
                     testicular RNA helicase; DEAD
                     (aspartate-glutamate-alanine-aspartate) box polypeptide
                     25; DEAD (Asp-Glu-Ala-Asp) box polypeptide 25; DEAD
                     (Asp-Glu-Ala-Asp) box helicase 25"
                     /codon_start=1
                     /product="ATP-dependent RNA helicase DDX25"
                     /protein_id="NP_113818.2"
                     /db_xref="GeneID:58856"
                     /db_xref="RGD:68381"
                     /translation="MASLLWGGDAGAAESERLNSHFSNLVHPRKNLRGIRSTTVPNID
                     GSLNTEEDDDEDDVVDLAANSLLNKLIRQSLVESSHRVEVLQKDPSSPLYSVKTFEEL
                     RLKEELLKGIYAMGFNRPSKIQEMALPMMLAHPPQNLIAQSQSGTGKTAAFVLAMLNR
                     VNALELFPQCLCLAPTYELALQTGRVVERMGKFCVDVEVMYAIRGNRIPRGTDVTKQI
                     VIGTPGTVLDWCFKRKLIDLTKIRVFVLDEADVMIDTQGFSDQSIRIQRALPSECQML
                     LFSATFEDSVWQFAERIIPDPNVIKLRKEELTLNNIRQYYVLCENRKDKYQALCNIYG
                     GITIGQAIIFCQTRRNAKWLTVEMMQDGHQVSLLSGELTVEQRASIIQRFRDGKEKVL
                     ITTNVCARGIDVKQVTIVVNFDLPVNQSEEPDYETYLHRIGRTGRFGKKGLAFNMIEV
                     DKLPLLMKIQDHFNSSIKQLDPEDMDEIEKIEY"
     exon            89..155
                     /gene="Ddx25"
                     /gene_synonym="GRTH"
                     /inference="alignment:Splign:2.1.0"
     exon            156..200
                     /gene="Ddx25"
                     /gene_synonym="GRTH"
                     /inference="alignment:Splign:2.1.0"
     exon            201..336
                     /gene="Ddx25"
                     /gene_synonym="GRTH"
                     /inference="alignment:Splign:2.1.0"
     exon            337..429
                     /gene="Ddx25"
                     /gene_synonym="GRTH"
                     /inference="alignment:Splign:2.1.0"
     exon            430..532
                     /gene="Ddx25"
                     /gene_synonym="GRTH"
                     /inference="alignment:Splign:2.1.0"
     exon            533..647
                     /gene="Ddx25"
                     /gene_synonym="GRTH"
                     /inference="alignment:Splign:2.1.0"
     exon            648..825
                     /gene="Ddx25"
                     /gene_synonym="GRTH"
                     /inference="alignment:Splign:2.1.0"
     exon            826..1063
                     /gene="Ddx25"
                     /gene_synonym="GRTH"
                     /inference="alignment:Splign:2.1.0"
     exon            1064..1226
                     /gene="Ddx25"
                     /gene_synonym="GRTH"
                     /inference="alignment:Splign:2.1.0"
     exon            1227..1415
                     /gene="Ddx25"
                     /gene_synonym="GRTH"
                     /inference="alignment:Splign:2.1.0"
     exon            1416..1618
                     /gene="Ddx25"
                     /gene_synonym="GRTH"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 gggggcgaga gcagagaccg cagctatggc gtcgttactt tggggaggcg acgctggggc
       61 ggcggagagc gagcggctga acagccactt ttcaaatctc gtccacccca ggaagaacct
      121 tcgtggtatt aggagtacca cagtcccaaa catagacggc tctcttaaca cggaagaaga
      181 tgatgacgaa gacgatgtag tggatttggc agccaattca ctcttaaaca agttaatccg
      241 ccagtcctta gtagaatcca gccaccgagt ggaagtctta caaaaggatc ccagctctcc
      301 actctactcg gtgaaaacat ttgaagagct acgactaaag gaagagttac taaaagggat
      361 ttatgcaatg ggatttaacc gaccatccaa aatccaagag atggccctcc ccatgatgct
      421 cgcacatcct ccccagaacc tcatagcaca gagccagtcc ggcacaggaa aaaccgctgc
      481 ctttgtctta gcgatgctaa acagagtgaa tgccttggaa ttgtttccac agtgcctctg
      541 cctggctccc acctatgaac tggctctgca gaccggccgc gtggttgaaa gaatggggaa
      601 attctgtgtg gatgttgaag tgatgtacgc cattcgagga aaccgaattc ctaggggcac
      661 cgacgtcacc aaacagattg tcatcggcac tcctgggact gtcctagact ggtgcttcaa
      721 gcggaaactg attgacttga caaagatccg tgtgtttgtc ctggatgaag cagatgtgat
      781 gattgacacc caaggattct ccgatcagag catccgtatc caaagagctt taccctctga
      841 atgccagatg ctcctctttt cagcaacctt tgaggactct gtgtggcagt ttgctgaacg
      901 aatcattcct gaccccaatg ttatcaagtt gcgaaaagag gaactgaccc tgaacaacat
      961 ccggcagtat tacgtgttgt gtgagaacag gaaagacaaa taccaagccc tctgtaatat
     1021 ctacggtggc atcaccattg gccaagccat catcttctgc cagacgcgtc ggaatgccaa
     1081 gtggctgaca gtggagatga tgcaggatgg ccaccaggtg tccctgctaa gtggagaact
     1141 cacggtggag cagcgggctt ccatcattca gaggttccga gatggcaaag agaaggttct
     1201 cataacaacc aatgtctgtg ctcgaggaat cgatgtgaag caggtcacca tcgttgtgaa
     1261 ctttgacctc cctgtaaacc agtcagaaga gccagactat gagacctacc tccaccgtat
     1321 agggcggacg ggacgctttg ggaaaaaagg tctcgccttc aacatgattg aagtggataa
     1381 gctgcccctg ctcatgaaaa tacaagacca cttcaatagc agtattaagc agctggaccc
     1441 agaagacatg gatgagattg aaaagatcga atattgaaga gagagaggcc tttgttgttt
     1501 gtgggctgtc ctagttgatg tgagaatgtt tgagcagagt ttgaagataa tggtctatgt
     1561 tgaggctctg tcacgtgaaa ctgtcacaga taacaaataa atgccatggc tctcctcaaa
     1621 aaaaaaaaaa
//
