LOCUS       NM_001318416            2161 bp    mRNA    linear   ROD 24-JUN-2024
DEFINITION  Rattus norvegicus DDHD domain containing 2 (Ddhd2), mRNA.
ACCESSION   NM_001318416
VERSION     NM_001318416.1
KEYWORDS    RefSeq.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 2161)
  AUTHORS   Araki,M., Ohshima,N., Aso,C., Konishi,A., Obinata,H., Tatei,K. and
            Izumi,T.
  TITLE     Enzymatic characterization of recombinant rat DDHD2: a soluble
            diacylglycerol lipase
  JOURNAL   J Biochem 160 (5), 269-279 (2016)
   PUBMED   27198176
  REMARK    GeneRIF: These results therefore support the idea that DDHD2
            functions as a DG lipase in vivo and produces 2-AG.
REFERENCE   2  (bases 1 to 2161)
  AUTHORS   Aso,C., Araki,M., Ohshima,N., Tatei,K., Hirano,T., Obinata,H.,
            Kishi,M., Kishimoto,K., Konishi,A., Goto,F., Sugimoto,H. and
            Izumi,T.
  TITLE     Protein purification and cloning of diacylglycerol lipase from rat
            brain
  JOURNAL   J Biochem 159 (6), 585-597 (2016)
   PUBMED   26790472
  REMARK    GeneRIF: DG lipase activity in the soluble fraction of rat brain is
            derived from DDHD2. DDHD2 is distributed widely in the rat brain.
            Immunohistochemical analysis revealed that DDHD2 is expressed in
            hippocampal neurons, but not in glia.
REFERENCE   3  (bases 1 to 2161)
  AUTHORS   Inloes,J.M., Hsu,K.L., Dix,M.M., Viader,A., Masuda,K., Takei,T.,
            Wood,M.R. and Cravatt,B.F.
  TITLE     The hereditary spastic paraplegia-related enzyme DDHD2 is a
            principal brain triglyceride lipase
  JOURNAL   Proc Natl Acad Sci U S A 111 (41), 14924-14929 (2014)
   PUBMED   25267624
REFERENCE   4  (bases 1 to 2161)
  AUTHORS   Baba,T., Kashiwagi,Y., Arimitsu,N., Kogure,T., Edo,A., Maruyama,T.,
            Nakao,K., Nakanishi,H., Kinoshita,M., Frohman,M.A., Yamamoto,A. and
            Tani,K.
  TITLE     Phosphatidic acid (PA)-preferring phospholipase A1 regulates
            mitochondrial dynamics
  JOURNAL   J Biol Chem 289 (16), 11497-11511 (2014)
   PUBMED   24599962
COMMENT     PROVISIONAL REFSEQ: This record has not yet been subject to final
            NCBI review. The reference sequence was derived from AB914679.1.
            
            [WARNING] On Mar 18, 2025 this sequence was replaced by
            NM_001318416.2.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AB914679.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMD00132261, SAMD00132262
                                           [ECO:0000348]
            ##Evidence-Data-END##
FEATURES             Location/Qualifiers
     source          1..2161
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="W"
                     /db_xref="taxon:10116"
                     /chromosome="16"
                     /map="16q12.4"
     gene            1..2161
                     /gene="Ddhd2"
                     /note="DDHD domain containing 2"
                     /db_xref="GeneID:680971"
                     /db_xref="RGD:1585818"
     exon            1..55
                     /gene="Ddhd2"
                     /inference="alignment:Splign:2.1.0"
     exon            56..281
                     /gene="Ddhd2"
                     /inference="alignment:Splign:2.1.0"
     CDS             62..2161
                     /gene="Ddhd2"
                     /function="lipase"
                     /codon_start=1
                     /product="phospholipase DDHD2"
                     /protein_id="NP_001305345.1"
                     /db_xref="GeneID:680971"
                     /db_xref="RGD:1585818"
                     /translation="MSSGESYQEQLSQSDPSPSPNSSSSFELLDMDVGSSYEPVSPHW
                     FYCKVIDSKELWIPFNSEDSQQLEEAHGSGKDCNERVVPTDGGRYDVHLGERMRYAVY
                     WDELPSEVRRCTWFYKGDKDNKYVPYSESFSQVLEDTYMLAVTLDEWKKKLESPNREI
                     IVLHNPKLMVHYQPITGSDEWGSTSTEQGRPRSVKRGVESIPVDIHCGEPLQIDHLVF
                     VVHGIGPACDLRFRSIVQCVNDFRSVSLNLLQTHFKKAQENQQIGRVEFLPVNWHSPL
                     HSTGVDIDLQRITLPSINRLRHFTNDTILDVFFYNSPTYCQTIVDTVASEMNRIYTLF
                     LQRNPDFKGGVSIAGHSLGSLILFDILTNQKDSIGDIDSEKGSLSTAEDLGDVSTLEE
                     ELKKLQLSEFVTVFEKEKVDREALALCTDRDLQEMGIPLGPRKKILNHFSATKSSVGI
                     NRPTMSASEVNIPKEKGDYLDVGIGQVSVKYPRLNYKPEIFFAFGSPIGMFLTVRGLR
                     RIDPNYKFPTCKGFFNIYHPFDPVAYRIEPMVVPGVEFEPMLIPHHKGRKRMHLELRE
                     GLTRMSMDLKNNLLGSLRMAWKSFTRAPYPALQASEMAEDTEAESEPSSEKPSDANTE
                     EPPVEVKEEAPINVGMLNGGQRIDYVLQEKPIESFNEYLFALQSHLCYWESEDTVLLV
                     LKEIYQTQGIFLDQPLQ"
     exon            282..472
                     /gene="Ddhd2"
                     /inference="alignment:Splign:2.1.0"
     exon            473..562
                     /gene="Ddhd2"
                     /inference="alignment:Splign:2.1.0"
     exon            563..683
                     /gene="Ddhd2"
                     /inference="alignment:Splign:2.1.0"
     exon            684..773
                     /gene="Ddhd2"
                     /inference="alignment:Splign:2.1.0"
     exon            774..909
                     /gene="Ddhd2"
                     /inference="alignment:Splign:2.1.0"
     exon            910..1118
                     /gene="Ddhd2"
                     /inference="alignment:Splign:2.1.0"
     exon            1119..1186
                     /gene="Ddhd2"
                     /inference="alignment:Splign:2.1.0"
     exon            1187..1309
                     /gene="Ddhd2"
                     /inference="alignment:Splign:2.1.0"
     exon            1310..1405
                     /gene="Ddhd2"
                     /inference="alignment:Splign:2.1.0"
     exon            1406..1489
                     /gene="Ddhd2"
                     /inference="alignment:Splign:2.1.0"
     exon            1490..1645
                     /gene="Ddhd2"
                     /inference="alignment:Splign:2.1.0"
     exon            1646..1748
                     /gene="Ddhd2"
                     /inference="alignment:Splign:2.1.0"
     exon            1749..1919
                     /gene="Ddhd2"
                     /inference="alignment:Splign:2.1.0"
     exon            1920..2079
                     /gene="Ddhd2"
                     /inference="alignment:Splign:2.1.0"
     exon            2080..2161
                     /gene="Ddhd2"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 ctcggctccc tccgcaaggg ctgagagcgc gagcggcgcg gacaagcgaa gccagagcga
       61 aatgtcatca ggggaatcgt accaggagca gctgtcacag tcagatccat ccccctcccc
      121 aaactccagt agttccttcg agctgctaga catggatgtt ggcagttcct acgaacccgt
      181 ttctccacat tggttttatt gtaaggtaat agactctaag gaactctgga ttcctttcaa
      241 ctctgaagat tcacagcagc tggaagaagc acatggctct ggaaaggatt gtaatgagag
      301 agttgtcccc actgatgggg gcagatatga tgtccatctc ggggagagga tgcggtatgc
      361 cgtgtactgg gatgaactac catcagaagt gagacggtgc acgtggtttt acaagggaga
      421 caaagacaat aagtacgtcc cctactccga gagcttcagc caagttttgg aggatacata
      481 tatgcttgcg gtaactctgg atgaatggaa aaaaaaacta gagtctccaa acagagaaat
      541 tattgtatta cacaacccaa agctcatggt gcattaccag ccaatcacag ggtctgatga
      601 atggggttca acatccactg agcaaggtcg gccaagatca gtaaaaagag gcgttgagag
      661 catccctgtt gatattcact gtggggaacc tttacaaata gatcacttag tgtttgtagt
      721 ccatgggatt ggaccagctt gtgatctccg ttttcgaagc attgttcaat gtgttaatga
      781 ttttcgaagt gtttccttga acctgctaca aacacatttt aagaaagccc aagaaaacca
      841 gcagattgga agggtagagt tccttccggt caactggcac agccctttgc attctactgg
      901 agtagatata gatctgcagc ggataaccct acccagcatc aatcgcctta gacacttcac
      961 taacgataca attctggatg tcttcttcta caatagcccc acctactgtc agactattgt
     1021 ggacacagta gcttctgaaa tgaaccgaat atatacactt tttctgcaga ggaaccctga
     1081 tttcaaaggg ggtgtatcca ttgctggtca tagtttaggt tcgcttatat tgtttgatat
     1141 cctaacaaat cagaaagatt ctattgggga tattgacagt gaaaagggtt cattgagtac
     1201 tgctgaggat ctaggagatg tatcaacatt agaagaagaa ctgaagaagc ttcagctctc
     1261 tgagtttgtc actgtctttg agaaggagaa agttgacaga gaagctctgg ctttatgtac
     1321 agacagagat cttcaggaaa tggggattcc cttaggaccc agaaagaaga tactgaacca
     1381 cttcagtgcc acaaagagtt cagtgggtat taatagacca accatgtcag cttctgaggt
     1441 aaatatcccc aaggaaaaag gtgactattt ggatgttgga attgggcagg tgtctgtaaa
     1501 atacccccgg cttaactaca aaccagaaat attctttgcc tttggatctc ccattggaat
     1561 gtttcttact gtccgaggat taagaagaat tgatccaaat tataaatttc ccacatgcaa
     1621 aggtttcttc aatatttacc atccttttga tcctgtggca tataggattg aaccaatggt
     1681 ggtcccagga gtagaatttg agccaatgct gattccacat cataaaggca ggaagcggat
     1741 gcacttagaa ctgagagagg gcttgaccag gatgagtatg gacctgaaga acaacttgct
     1801 gggttcactg aggatggcct ggaagtcttt caccagagct ccataccctg ccttacaagc
     1861 ttcagaaatg gcagaagaca ctgaggcaga atccgaacca agttcagaaa agcccagtga
     1921 cgccaacaca gaagagcctc ctgtggaggt caaagaggag gctcccatca atgtgggaat
     1981 gctgaatgga ggccagcgca ttgactatgt gttacaggag aagcccattg agagttttaa
     2041 tgaatattta tttgctctac aaagccatct atgctactgg gaatctgaag atacagtact
     2101 gcttgtcctc aaagagatct accaaaccca ggggatcttc cttgatcagc ctttacagta
     2161 a
//
